14TSB_ESAP: Non-invasive photonic sensors for detection of latent fungal infections and internal rot or disorders in fruit

Lead Research Organisation: East Malling Research (United Kingdom)
Department Name: Science

Abstract

This project aims to develop a new form of non-invasive, real-time quality control system to improve production efficiency in agri-food industries. A new type of optical based analsyer which will be able to differentiate between healthy fruit, and fruit with internal disorders such as rot or latent infection, will be developed. The proposed instrument will be easily integrated into existing packaging line systems and aims to provide early detection of infected or spoiled fruit, such that this fruit can be marketed early or discarded to prevent the spread of further infection. Overall, this type of quality control with result in a new method of crop management, increasing production efficiency and yields, and therefore benefiting the UK and worldwide markets.

Technical Summary

This proposal will use a photonics-based approach for detection of volatile biomarkers for fruit quality monitoring, enabling appropriate action to be taken by producers to minimise losses. This includes (a) the early detection of latent fungal infection pre-harvest to enable earlier marketing of fruit to reduce losses in apples, cherries and plums and (b) post harvest detection of internal fungal rots and physiological disorders prior to packaging and marketing. This will be achieved by developing an integrated photonics based QA detection system which can be used in the packing line prior to storage, or after storage, to minimise economic losses. Furthermore, it may also prevent fruit with high concentration of mycotoxins infected by a Fusarium species from entering the food chain. Overall this non-invasive monitoring approach will result in a reduction of crop wastage, improved value for money in post-harvest treatments, reduction in grading cost after post-harvest storage and before marketing, reduction in environmental impacts and will maximise yield and profit.

Planned Impact

This project will generate results that will contribute to:
(a) maximising marketable yield and profit potential. This proposal will develop an photonics-based solution for the early detection and quantification of latent fungal infection before post-harvest storage;
(b) reducing crop losses, hence increasing profits;
(c) improving value for money in post-harvest treatments;
(d) reducing grading cost after post-harvest storage before marketing;
(e) minimising potentially negative environmental impacts associated with food production;
(f) developing innovative engineering tools to optimise market value of UK perishable food chains;
(g) offer practical devices which can be utilised in packing lines and post-harvest prior to packaging for improved QA and reduction in losses.
The photonics-based system developed in this proposal may be applicable to other fruit and vegetables although further research is necessary to derive classification criteria based on volatile profiles.
The social benefits of this project also include the potential increase in quality of marketed fruit and even reduced cost to the consumer through greater efficiency in production, both of these benefits contributing to overall benefits to public health.

Publications

10 25 50
 
Description Initial research on sampling schemes showed the importance of the relative size and variability of volatiles that can differentiate healthy from fruit with latent fungal infection in affecting detection/sampling protocols. We have determined several key biomarkers which are produced in differential contractions between healthy fruit and those plum, cherry and strawberry fruit with latent fungal infection (we are not able to reveal the identities of these biomarkers because of confidentiality at this moment). Using this information, the industry partner built a prototype instrument to detect these biomarkers. We have carried out a series of evaluation trials, demonstrating that this instrument can operate in the commercial packing houses, and can detect these biomarkers without the need for actively sampling air. However, further development and evaluation is necessary in order to test against fruit produced during the main season.
Exploitation Route The results have already been used by the lead industry partner to develop a prototype instrument. We have already carried a number of evaluation trials. The two industry partners are currently discussing the way forward. Depending on the discussion outcome, we may publish the results in peer-reviewed journals.
Sectors Agriculture

Food and Drink

 
Description Three biomarkers were used by Cascade to develop a prototype instrument for detecting fruit with latent fungal infection. Our evaluation results will inform Cascade how to take this knowledge forward.
First Year Of Impact 2018
Sector Agriculture, Food and Drink
Impact Types Economic

 
Description An optical detector for latent fungal infection in produce
Amount £183,272 (GBP)
Funding ID BB/X003744/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2022 
End 10/2023
 
Description Horticulture: predicting strawberry fruit infection by Mucor and Rhizopus using climatic conditions and pathogen inoculum levels
Amount £50,401 (GBP)
Funding ID BB/X01181X/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 05/2023 
End 11/2023
 
Description Use of volatile to detect fruit with latent fungal infection 
Organisation Berry Gardens Growers Limited
Country United Kingdom 
Sector Private 
PI Contribution We collected volatile from many samples for fruit: cherry, plum and strawberry; Cranfield University analysed the samples in GCMS; we identified biomarkers for fruit with latent fungal infection. Together with BGG, we carried out numerous trials to evaluate a prototype instrument.
Collaborator Contribution Cascade built a prototype instrument that detected the biomarkers. BGG provided fruit and carried out fruit rotting assessment during the evaluation phase
Impact We identified the biomarkers and Cascade built a prototype instrument. Initial evaluation trials suggested that further development is needed before it can be used commercially. Currently, Cascade and BGG are discussing whether they could this forward.
Start Year 2014
 
Description Use of volatile to detect fruit with latent fungal infection 
Organisation Cascade Technologies
Country United Kingdom 
Sector Private 
PI Contribution We collected volatile from many samples for fruit: cherry, plum and strawberry; Cranfield University analysed the samples in GCMS; we identified biomarkers for fruit with latent fungal infection. Together with BGG, we carried out numerous trials to evaluate a prototype instrument.
Collaborator Contribution Cascade built a prototype instrument that detected the biomarkers. BGG provided fruit and carried out fruit rotting assessment during the evaluation phase
Impact We identified the biomarkers and Cascade built a prototype instrument. Initial evaluation trials suggested that further development is needed before it can be used commercially. Currently, Cascade and BGG are discussing whether they could this forward.
Start Year 2014
 
Description International Workshop on stone fruit brown rot 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact This is an international group specialized in biology and management of brown rot on stone fruit, involving both researchers, advisers and key producer organisations. The meeting is to exchange research outcomes, review future research needs, and how to translate the research into practice
Year(s) Of Engagement Activity 2015