Immunology: Understanding the molecular and cellular basis of Immunity: Somatic Hypermutation and Affinity Maturation
Lead Research Organisation:
Babraham Institute
Department Name: UNLISTED
Abstract
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Technical Summary
The objectives address the molecular mechanisms that facilitate antibody diversification through the somatic mutation of antibody encoding genes in B cells.
Planned Impact
unavailable
Organisations
Publications
| Description | Two studies have been submitted and are available as preprint. Each is undergoing an extended period of peer review We validated our novel mouse line where an auxin-induced degron is tagged to the N-terminus of endogenous Ddx3x. We found that Ddx3x protein levels could be halved in just 30 mins upon addition of auxin, in primary B-cell cultures. We confirmed a reduction in protein synthesis activity in these cells, which we are now investigating by profiling ribosome binding using Ribo-Seq. Further to our immediate research progress, our new tool was instrumental for the demonstration that in transgenic mice the effect of tag fusions on protein expression is cell- and tissue-context dependent (manuscript submitted). This work was done in collaboration with the Degron Tagging Cluster of the MRC National Mouse Genetics Network led by Dr. Andrew Wood (University of Edinburgh). |
| Exploitation Route | too early to say |
| Sectors | Manufacturing including Industrial Biotechology |