Investigating the druggability of the motif-mediated interactome with peptide PROTACs
Lead Research Organisation:
Institute of Cancer Research
Department Name: Division of Cancer Biology
Abstract
Short, Linear Motif (SLiMs) are ubiquitous compact interaction interfaces that direct key cellular processes. PROTACs targeting SLiM-binding pockets can specifically promote ubiquitin ligase-dependent degradation of regulatory proteins in critical cellular pathways. We will apply a scalable method using genetically encoded peptide PROTACs to provide proof of principle for the therapeutic relevance of targeted degradation of essential proteins. Peptide PROTACs provide a fast and cost-effective approach to mirror the mode of action of a small molecule PROTAC, providing evidence for the spatiotemporal compatibility of the E3 and target, and the phenotype of target protein degradation.
People |
ORCID iD |
Norman Davey (Primary Supervisor) | |
Maria Portillo Malumbres (Student) |
Studentship Projects
Project Reference | Relationship | Related To | Start | End | Student Name |
---|---|---|---|---|---|
MR/R01583X/1 | 30/09/2018 | 29/09/2025 | |||
2440757 | Studentship | MR/R01583X/1 | 04/10/2020 | 03/10/2024 | Maria Portillo Malumbres |
Title | RPE1_AAVS1_TRE3GS_FRT cell line |
Description | hTERT RPE-1 stable cell line with a TetOn3G transactivator - FRT insertion site - Blasticidin resistance construct integrated at the AAVS1 locus . Engineered using CRISPR/Cas(D10A) nickase nuclease. |
Type Of Material | Cell line |
Year Produced | 2022 |
Provided To Others? | No |
Impact | RPE1_AAVS1_TRE3GS_FRT cell line allows inducible and dose-dependent expression of a gene of interest in a reproducible manner while ensuring minimal alteration of the genome |