Understanding the molecular determination of quality traits in citrus fruit (MARTIN_J17ICASE)
Lead Research Organisation:
University of East Anglia
Department Name: Graduate Office
Abstract
Citrus fruit has been cultivated in an ever-widening area since ancient times; the best-known examples are oranges, lemons, grapefruit, and limes. Most commercial citrus varieties are interspecific hybrids which are propagated vegetatively, meaning that breeding and crop improvement are very difficult. One of the most important traits in citrus fruit is acidity, which is a positive attribute in varieties such as lemon, but a negative trait in the true species, mandarin.
To understand the genetic determinants of fruit acidity at the molecular level, and to facilitate improvement through breeding where possible, we are investigating the genetic determinants of fruit acidity in citrus. This studentship will extend our understanding of how this trait is determined, by developing a functional bioassay for acidity, and using this to explore the activity of genes from different citrus germplasm accessions in determining fruit acidity. We aim to create new low acidity lines of commercial citrus varieties by genome editing, in co-operation with our industrial partners. These low acidity varieties could be used to produce low acidity, low added sugar citrus juices.
To understand the genetic determinants of fruit acidity at the molecular level, and to facilitate improvement through breeding where possible, we are investigating the genetic determinants of fruit acidity in citrus. This studentship will extend our understanding of how this trait is determined, by developing a functional bioassay for acidity, and using this to explore the activity of genes from different citrus germplasm accessions in determining fruit acidity. We aim to create new low acidity lines of commercial citrus varieties by genome editing, in co-operation with our industrial partners. These low acidity varieties could be used to produce low acidity, low added sugar citrus juices.
People |
ORCID iD |
Cathie Martin (Primary Supervisor) | |
Elliott Atkins (Student) |
Studentship Projects
Project Reference | Relationship | Related To | Start | End | Student Name |
---|---|---|---|---|---|
BB/M011216/1 | 30/09/2015 | 31/03/2024 | |||
1940561 | Studentship | BB/M011216/1 | 30/09/2017 | 30/03/2022 | Elliott Atkins |
NE/W503034/1 | 31/03/2021 | 30/03/2022 | |||
1940561 | Studentship | NE/W503034/1 | 30/09/2017 | 30/03/2022 | Elliott Atkins |
Description | British Science Association stand - Norwich Science Treasure Hunt 2018 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | Engaged the general public, particularly young children, in stall activities surrounding sustainable living, including food and energy production. |
Year(s) Of Engagement Activity | 2018 |
Description | DNA Necklace Workshop - East Norfolk Triple Science Network day at JIC 2018 |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Schools |
Results and Impact | Supported a DNA necklace workshop at the John Innes Centre where school children were taught DNA basics through an engaging activity. The children extract their own DNA from cheek cells, and store their precipitated DNA in vials, which they can take home on necklaces. |
Year(s) Of Engagement Activity | 2018 |
Description | DNA Necklace Workshop - Sewell Park School visit 2018 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Schools |
Results and Impact | Personally led a DNA necklace workshop at the John Innes Centre where school children were taught DNA basics through an engaging activity. The children extract their own DNA from cheek cells, and store their precipitated DNA in vials, which they can take home on necklaces. |
Year(s) Of Engagement Activity | 2018 |
Description | DNA fingerprinting and forensics workshop - Sixth Form open day at UEA 2018 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Schools |
Results and Impact | Supported a workshop at a Sixth Form open day at the University of East Anglia 2018. Students were guided to prepare and perform a DNA restriction digestion assay and gel electrophoresis to determine which "suspect" was present at the scene of a crime. Water samples from various possible "crime scenes" were also compared by viewing micro-flora and fauna communities using microscopes. |
Year(s) Of Engagement Activity | 2018 |
Description | Food facts workshop - School visit in Lowestoft 2018 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Schools |
Results and Impact | Designed and led a workshop to inform students about healthy eating habits with engaging science activities. These included crudely purifying gluten in different types of dough and comparing the relative elasticity, and also using a coloured pH scale and litmus paper to compare acidity in various drinks and liquid products. |
Year(s) Of Engagement Activity | 2018 |
Description | Ocean Plankton workshop - Bringing Scientists To You event at Thetford Academy 2018 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Schools |
Results and Impact | Supported a workshop regarding the importance of our ocean life, particularly plankton, and how this relates to our dependence on ocean health. |
Year(s) Of Engagement Activity | 2018 |
Description | Question Time session - East Norfolk Triple Science Network day at JIC 2018 |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Schools |
Results and Impact | Attended a scientist "question time" session, as a member of the panel, where school children could ask questions regarding life inside and outside of the lab. |
Year(s) Of Engagement Activity | 2018 |
Description | SAW Trust stand at Boomtown Festival 2019 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | Engaged the general public, particularly young children attending the Boomtown Festival 2019, in activities surrounding micro-plastic pollution, biodegradable plastic-alternatives and sustainable energy production. |
Year(s) Of Engagement Activity | 2019 |
Description | SAW Trust/OpenPlant stand - Norwich Science Festival 2018 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | Volunteered to help an OpenPlant stand at the 2018 Norwich Science Festival. We primarily engaged younger children in activities surrounding synthetic and molecular biology, such as the pathway from DNA to protein. |
Year(s) Of Engagement Activity | 2018 |