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JIC-CAS Joint Centre

Lead Research Organisation: John Innes Centre
Department Name: UNLISTED

Abstract

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Technical Summary

This project will leverage the complementary strengths of world-leading plant science institutes in the UK and China to address sustainable agricultural development challenges of the developing world. In particular to:
• develop new varieties of wheat with improved yield potential;
• improve the resistance of cereal crops to drought and salt-stress;
• exploit high value medicinal bioactives from Chinese plants in African agriculture.

Planned Impact

unavailable
 
Description 1. We are studying the functions of a member of the DA1 family of growth regulators that confers resistance to the oomycete pathogen Albugo. This is done in collaboration with Jonathan Jones in The Sainsbury Laboratory. We have shown that this family member, DAR4, is an integrated decoy molecule using domain swaps with other family members. Mutations that influence the growth-regulating activities of DA1, the type member of the family, trigger HR when introduced into DAR4. The guiding hypothesis is that an Albugo effector molecule(s) influences DA1 family function, and such influences are detected by DAR4 to trigger immunity. Currently we are assessed if mutations in DA1 and DAR1 influence pathogen growth and if pathogens influence plant growth. 2. The characterisation of the biosynthetic pathway for bioactive flavones in Scutellaria baicalensis continues. We have discovered two genes encoding P450 enzymes that catalyse a 6-hydroxylation and an 8-hydroxylation. This work was published in Molecular Plant in July 2017 to accompany the Science Advances paper we had published in 2016. We have characterised a family of 12 O-methyl transferases which are involved in the decoration of 4' deoxyflavones, and identified 3 which can singly or in combination account for the synthesis of wogonin from norwogonin and for the synthesis of other methylated 4'deoxyflavones which are minor metabolites in S.baicalensis roots.
We have completed the genome sequence for S.baicalensis and identified a number of candidate transcription factors regulating the 4'deoxyflavone biosynthetic pathway in roots. We undertook a collecting trip for new Scutellaria species in Yunan province in May 2017 and added 3 new species to the living collection in Chenshan Botanical Garden.
3. Early in the project a paper reported the role of these transporter genes and vacuolar nitrate storage energised by the tonoplast ATPase and PPase pumps in nitrogen use efficiency (NUE) in Arabidopsis and Brassica napus [Han et al. 2016 Plant Physiology 170:1684-1698]. This work demonstrated that the key hypotheses we had proposed are correct and we therefore revised the work plan to focus specifically on the role of the vacuolar H+-PPase (AVP1). Splice variants of AVP1 have been identified and these are being functionally characterized in yeast and their role in nitrogen storage and stress responses is being assessed in plants.
Exploitation Route Primarily by publication in high impact factor journals. There is also interest from a biotechnology park in China (Kotler Medical Park) with money to invest in trials of bioactives from Chinese Traditional Medicine. Discussions have started with Zhi Zhang of LifeArc a UK-based company interested in investing in human trials of 4' deoxyflavones as a therapy complementary to traditional therapies.
The work on the evolutionary pathway of 4' deoxyflavone production in the genus Scutellaria will form part of a closer link with Alan Paton at Kew Gardens.
Plants expressing the splice variants of AVP1 have been generated and these will be important for assessing the importance of the pumps in crops.
Sectors Agriculture

Food and Drink

Pharmaceuticals and Medical Biotechnology

 
Description Pathogens influence many aspects of plant growth and development. We aim to understand the role of DA1 family members in responding to pathogens and influencing growth
First Year Of Impact 2017
Sector Agriculture, Food and Drink
Impact Types Economic

 
Description CEPAMS Symposium 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Symposium reporting CEPAMS activities with International guest speakers as well
Year(s) Of Engagement Activity 2018
 
Description CEPAMS Symposium Shanghai 2016 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact CEPAMS Symposium Shanghai 2016 Friday 18th March
Editing Long Distance Transport and Storage to Optimize the Site of Nitrogen Assimilation in Drought and Salt-stress
Year(s) Of Engagement Activity 2016
 
Description I was keynote speaker at the Chenshan Meeting on specialised metabolism in plants 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was keynote speaker at the Chenshan Meeting on specialised metabolism in plants
Year(s) Of Engagement Activity 2018
 
Description I was keynote speaker at the Hainan first National Meeting on specialised Metabolomics 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact I was a keynote speaker at the Hainan Meeting on metabolomics
Year(s) Of Engagement Activity 2018
 
Description International Symposium on Plant Membrane Biology, Annapolis MD, USA 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact International Symposium on Plant Membrane Biology, Annapolis MD, 2016
Thursday 9th June 2016 S16 Future Perspectives
Year(s) Of Engagement Activity 2016
 
Description Invited Seminar at Bristol University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited seminar speaker at Bristol University - title 'The Plant Lytic Vacuole: Space-Filler, Garbage Bag, or Something More Interesting?'
Year(s) Of Engagement Activity 2017
 
Description Invited Seminar at SIPPE Shanghai Institute of Plant Physiology and Ecology 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact 4th November 2016 in Shanghai 15:00
Invited Seminar at SIPPE Shanghai Institute of Plant Physiology and Ecology
Year(s) Of Engagement Activity 2016
 
Description Invited seminar speaker Padua Italy (12th January 2016) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Presentation on the role of the plant lytic vacuole.
Title: Lytic Vacuole: Space-Filler, Garbage Bag, or Something More Interesting?
Year(s) Of Engagement Activity 2016
 
Description Invited seminar speaker Plant Sciences Univiersity of Oxford, UK (January 28 th 2016) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited Departmental seminar speaker. Topic was the role of the lytic vacuole.
Title: The Plant Lytic Vacuole: Space-Filler, Garbage Bag, or Something More Interesting?
Year(s) Of Engagement Activity 2016
 
Description Invited speaker at SEB Plant Transport Group Meeting Glasgow 2014 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited presentation on the functional role of the vacuole proton pumping pyrophosphatase.
Year(s) Of Engagement Activity 2014
 
Description Invited speaker at York University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Invited speaker to York University: Plant Biology Seminar - The Plant Lytic Vacuole: Space-Filler, Garbage Bag, or Something More Interesting?
Year(s) Of Engagement Activity 2017
 
Description Invited speaker: Gordon Research Conference on Organellar Channels and Transporters 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited speaker: Gordon Research Conference on Organellar Channels and Transporters Gordon Research Conference on Organellar Channels and Transporters
Year(s) Of Engagement Activity 2017
 
Description Keynote Speech: Plants and Human Health at the Wellcome Trust China-UK Life Science Summit 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Keynote Speech: Plants and Human Health at the Wellcome Trust China-UK Life Science Summit
Year(s) Of Engagement Activity 2017
 
Description Member of the Synthetic Biology Leadership Council 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Member of the Synthetic Biology Leadership Council
Year(s) Of Engagement Activity 2017
 
Description Member of the UK Science Partnership for Animal and Plant Health 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Member of the UK Science Partnership for Animal and Plant Health - quarterly meetings are held
Year(s) Of Engagement Activity 2017
 
Description Member of the interviewing panel for Herchel Smith Professorship of Biochemistry 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Member of the interviewing panel for Herchel Smith Professorship of Biochemistry position - University of Cambridge
Year(s) Of Engagement Activity 2017
 
Description New Phytologist Next Generation Scientists meeting - Open Address 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Opening Address at New Phytologist Next Generation Scientists meeting - Scientific meeting
Year(s) Of Engagement Activity 2017
 
Description Nourishing ten billion sustainably: resilient food production in a time of climate change 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact CLIMATE CHANGE : SCIENCE AND SOLUTIONS | BRIEFING 10
The global food system accounts for around one third of greenhouse gas (GHG) emissions generated by human activity. It therefore offers a major opportunity for progress towards net zero if emissions can be reduced at the same time as delivering food security and building resilience to the inevitable impacts of climate change. Research shows
how solutions can be found in diet change, respectfully approached, sustainable agricultural practices and harnessing the continuing wave of innovation in food biotechnology
Year(s) Of Engagement Activity 2021
URL https://royalsociety.org/-/media/policy/projects/climate-change-science-solutions/climate-science-so...
 
Description Opening address at Breeders Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Opening address at Breeders Day at the John Innes Centre
Year(s) Of Engagement Activity 2017
 
Description Panel Meeting of Severo Ochoa and Maria de Maeztu Committee - Madrid, Spain 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Panel Meeting of Severo Ochoa and Maria de Maeztu Committee - Madrid, Spain
Year(s) Of Engagement Activity 2017
 
Description Panel discussion for the Royal Society for Hay Festival 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Hay Festival - panel discussion for the Royal Society
exploring the public attitudes to the range of applications that genetic technologies might have, and the differences in such attitudes depending on context. This might look at the difference in public opinion in areas like food and medicine, for example, or explore concepts like 'naturalness'
Year(s) Of Engagement Activity 2017
 
Description Plenary Speaker American Society for Pharmacognasy Lexington Kentucky USA 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I presented work on the Chinese medicinal preparation, Huang Qin
Year(s) Of Engagement Activity 2018
 
Description Royal Society fact finding visit to Beijing 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Royal Society fact finding visit to Beijing, 22 to 26 October for Royal Society's genetic technologies programme
Year(s) Of Engagement Activity 2017
 
Description Salt & Water Stress in Plants - Gordon conference presentation Hong Kong 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Report research to the scientific community
Year(s) Of Engagement Activity 2012
URL http://www.grc.org/programs.aspx?year=2012&program=salt
 
Description Scientific Advisory Board Member for Max PIanck Institute of Molecular Plant Physiology, Golm, Germany 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Scientific Advisory Board Meeting for Max PIanck Institute of Molecular Plant Physiology
Year(s) Of Engagement Activity 2017
 
Description Speaker at MfN workshop joint with Rothamsted Research 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I was a Speaker at the ISP MfN workshop joint with Rothamsted Research
Year(s) Of Engagement Activity 2018
 
Description University of Geneva - PhD Retreat 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact University of Geneva - PhD Retreat, participation in a debate: " Technological advances - A new chance for changing public perception on GMOs". Also speaker title: 'The plant lytic vacuole: space-filler, garbage bag, or something more interesting'. A two-day retreat for PhD students in molecular plant sciences at universities in French-speaking Switzerland.
Year(s) Of Engagement Activity 2017
 
Description World Life Science Conference (Sustainable Agriculture Session), Beijing, 2016 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact World Life Science Conference (Sustainable Agriculture Session), Beijing, 2016
3rd November 2016, Session 48 Sustainable Agriculture - Membrane Transporters and Nutrition - from crops to people
Year(s) Of Engagement Activity 2016
 
Description World Life Science Conference (Sustainable Agriculture Session), Beijing, 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact China National Convention Center presentation: The Plant Vacuole: Roles in Plant and Human Nutrition, and Cellular Signaling 27th - 29th October 2018
Year(s) Of Engagement Activity 2018