603: Integrated project: Arabidopsis Systems Biology
Lead Research Organisation:
University of Warwick
Department Name: Warwick HRI
Abstract
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Technical Summary
The overall aim of this project is to develop a systems approach to the study of three aspects of Arabidopsis biology, pathogen interactions (Peronospora and Botrytis), flowering and senescence. A user group approach generates advanced genomic resources and standardised datasets. This allows the parallel study of key regulatory networks operating simultaneously in the same biological system. We expect this approach to reveal new regulatory genes and insights into how these networks cross-talk and interact. The key resources and expertise will be developed to support and integrate the research sub-projects. Genetic resources includes a high throughput plant transformation pipeline, a coordinated cloning and vector construction pipeline to feed the plant transformation, yeast 2 hybrid and yeast 1 hybrid techniques. Amplification of 20K Arabidopsis promoters to generate a promoter chip for ChIP analysis. Large microarray datasets are being generated using CATMA slides. Highly replicated time course experiments with many time points for leaf development in long days, in short days, following transfer from short to long, following infection with Botrytis and Peronospora, will be used to generate models for gene expression. These will pinpoint key regulatory genes common or unique to the different treatments.
Planned Impact
unavailable
Organisations
People |
ORCID iD |
| Vicky Buchanan-Wollaston (Principal Investigator) |
| Description | The overall aim of this project was to develop a systems approach to the study of three aspects of Arabidopsis biology, pathogen interactions (Peronospora and Botrytis), flowering and senescence. The concept of the programme was to take a user group approach to generate advanced genomic resources and standardised datasets. Large microarray datasets were generated using CATMA slides. Highly replicated time course experiments with many time points for leaf development in long days, in short days, following transfer from short to long, following infection with Botrytis and Peronospora, were all used to generate time courses and models of gene expression. In the consequent PRESTA project we used these data sets to pinpoint key regulatory genes common or unique to the different treatments. |
| Exploitation Route | Understanding how plants regulate senescence and/or pathogen responses is potentially of great value to improve crop plants, to make them more tolerant to stress and hence improve yield and quality in less favourable growing environments. The methods and data generated in this project have been applicable in other experiments and will be useful in other plant species. We have since identified a core network of stress response genes that overlap responses to different stresses and may be good candidates for improving crop resilience to stress. |
| Sectors | Agriculture Food and Drink |
| Description | PRESTA: Plant Responses to Environmental Stress in Arabidopsis |
| Amount | £4,409,109 (GBP) |
| Funding ID | BB/F005806/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2008 |
| End | 04/2014 |