RNA virus Evolution
Lead Research Organisation:
The Pirbright Institute
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
RNA viruses are genetically very diverse. They quickly emerge and evolve with high mutation rates, large population sizes, and short generation times. This fellowship aims to increase understanding of genetic and phenotypic characteristics that impact upon RNA virus evolvability. Focusing in the first instance on Foot-and-mouth disease virus, the project will investigate the limits of genetic variability at a consensus level. The evolution of the consensus sequence shall also be investigated using the power of next generation sequencing technologies. Complementary to this, in vitro experiments will be undertaken in order to understand how genetic diversity is generated within a cell, and how this impacts upon virus fitness.
Planned Impact
unavailable
Organisations
- The Pirbright Institute (Lead Research Organisation)
- Botswana Vaccine Institute (Botswana) (Collaboration)
- FGBI Federal Centre for Animal Health (Collaboration)
- French Agency for Food, Environmental and Occupational Health & Safety (ANSES) (Collaboration)
- National Agri-Food Quality and Health Service (SENASA) (Collaboration)
- Lombardy and Emilia Romagna Experimental Zootechnic Institute (IZSLER) (Collaboration)
- Onderstepoort Veterinary Institute (Collaboration)
- Project Directorate on Foot and Mouth Disease (Collaboration)
- Lanzhou Veterinary Research Institute (Collaboration)
- Department of Livestock Development (Collaboration)
- U.S. Department of Agriculture USDA (Collaboration)
- Veterinary and Agrochemical Research Centre (Collaboration)
- National Centre for Foreign Animal Diseases (NCFAD) (Collaboration)
- Pan American Foot-and-Mouth Disease Center (Panaftosa) (Collaboration)
People |
ORCID iD |
| Donald King (Principal Investigator) |
Publications
Logan G
(2018)
Deep Sequencing of Foot-and-Mouth Disease Virus Reveals RNA Sequences Involved in Genome Packaging.
in Journal of virology
Maier HJ
(2013)
Visualizing the autophagy pathway in avian cells and its application to studying infectious bronchitis virus.
in Autophagy
Maier HJ
(2013)
Infectious bronchitis virus generates spherules from zippered endoplasmic reticulum membranes.
in mBio
| Description | RNA viruses are highly adept at evolving (through genetic mutation and selection) to take advantage of new environmental niches and to escape host immunity and antiviral activity. This fellowship post investigated technical and analytical approaches that can be applied to address questions relating the RNA virus evolution. |
| Exploitation Route | This fellowship is now completed |
| Sectors | Education |
| Description | OIE/FAO Laboratory Network for FMD |
| Organisation | Botswana Vaccine Institute |
| Country | Botswana |
| Sector | Public |
| PI Contribution | The Pirbright Institute currently coordinates a global network of fourteen International Reference Laboratories for FMD. |
| Collaborator Contribution | The Network of OIE/FAO FMD Reference Laboratories has been established with two principal goals: 1) To understand global virus distribution patterns and use these data to inform vaccine recommendations and 2) To harmonise and improve the quality of laboratory testing carried out by international and national reference laboratories. These activities require sharing and joint evaluation of surveillance information from laboratory diagnosis, serotyping, genetic characterisation and vaccine matching tests and harmonisation of standards for diagnostic procedures. |
| Impact | Outputs from the network provide vital information to international organisations involved in the control of FMD (such as OIE and FAO), as well as specific regional and national programmes to control FMD |
| Start Year | 2006 |
| Description | OIE/FAO Laboratory Network for FMD |
| Organisation | Department of Livestock Development |
| Country | Thailand |
| Sector | Public |
| PI Contribution | The Pirbright Institute currently coordinates a global network of fourteen International Reference Laboratories for FMD. |
| Collaborator Contribution | The Network of OIE/FAO FMD Reference Laboratories has been established with two principal goals: 1) To understand global virus distribution patterns and use these data to inform vaccine recommendations and 2) To harmonise and improve the quality of laboratory testing carried out by international and national reference laboratories. These activities require sharing and joint evaluation of surveillance information from laboratory diagnosis, serotyping, genetic characterisation and vaccine matching tests and harmonisation of standards for diagnostic procedures. |
| Impact | Outputs from the network provide vital information to international organisations involved in the control of FMD (such as OIE and FAO), as well as specific regional and national programmes to control FMD |
| Start Year | 2006 |
| Description | OIE/FAO Laboratory Network for FMD |
| Organisation | FGBI Federal Centre for Animal Health |
| Country | Russian Federation |
| Sector | Public |
| PI Contribution | The Pirbright Institute currently coordinates a global network of fourteen International Reference Laboratories for FMD. |
| Collaborator Contribution | The Network of OIE/FAO FMD Reference Laboratories has been established with two principal goals: 1) To understand global virus distribution patterns and use these data to inform vaccine recommendations and 2) To harmonise and improve the quality of laboratory testing carried out by international and national reference laboratories. These activities require sharing and joint evaluation of surveillance information from laboratory diagnosis, serotyping, genetic characterisation and vaccine matching tests and harmonisation of standards for diagnostic procedures. |
| Impact | Outputs from the network provide vital information to international organisations involved in the control of FMD (such as OIE and FAO), as well as specific regional and national programmes to control FMD |
| Start Year | 2006 |
| Description | OIE/FAO Laboratory Network for FMD |
| Organisation | French Agency for Food, Environmental and Occupational Health & Safety (ANSES) |
| Country | France |
| Sector | Public |
| PI Contribution | The Pirbright Institute currently coordinates a global network of fourteen International Reference Laboratories for FMD. |
| Collaborator Contribution | The Network of OIE/FAO FMD Reference Laboratories has been established with two principal goals: 1) To understand global virus distribution patterns and use these data to inform vaccine recommendations and 2) To harmonise and improve the quality of laboratory testing carried out by international and national reference laboratories. These activities require sharing and joint evaluation of surveillance information from laboratory diagnosis, serotyping, genetic characterisation and vaccine matching tests and harmonisation of standards for diagnostic procedures. |
| Impact | Outputs from the network provide vital information to international organisations involved in the control of FMD (such as OIE and FAO), as well as specific regional and national programmes to control FMD |
| Start Year | 2006 |
| Description | OIE/FAO Laboratory Network for FMD |
| Organisation | Lanzhou Veterinary Research Institute |
| Country | China |
| Sector | Learned Society |
| PI Contribution | The Pirbright Institute currently coordinates a global network of fourteen International Reference Laboratories for FMD. |
| Collaborator Contribution | The Network of OIE/FAO FMD Reference Laboratories has been established with two principal goals: 1) To understand global virus distribution patterns and use these data to inform vaccine recommendations and 2) To harmonise and improve the quality of laboratory testing carried out by international and national reference laboratories. These activities require sharing and joint evaluation of surveillance information from laboratory diagnosis, serotyping, genetic characterisation and vaccine matching tests and harmonisation of standards for diagnostic procedures. |
| Impact | Outputs from the network provide vital information to international organisations involved in the control of FMD (such as OIE and FAO), as well as specific regional and national programmes to control FMD |
| Start Year | 2006 |
| Description | OIE/FAO Laboratory Network for FMD |
| Organisation | Lombardy and Emilia Romagna Experimental Zootechnic Institute (IZSLER) |
| Country | Italy |
| Sector | Public |
| PI Contribution | The Pirbright Institute currently coordinates a global network of fourteen International Reference Laboratories for FMD. |
| Collaborator Contribution | The Network of OIE/FAO FMD Reference Laboratories has been established with two principal goals: 1) To understand global virus distribution patterns and use these data to inform vaccine recommendations and 2) To harmonise and improve the quality of laboratory testing carried out by international and national reference laboratories. These activities require sharing and joint evaluation of surveillance information from laboratory diagnosis, serotyping, genetic characterisation and vaccine matching tests and harmonisation of standards for diagnostic procedures. |
| Impact | Outputs from the network provide vital information to international organisations involved in the control of FMD (such as OIE and FAO), as well as specific regional and national programmes to control FMD |
| Start Year | 2006 |
| Description | OIE/FAO Laboratory Network for FMD |
| Organisation | National Agri-Food Quality and Health Service (SENASA) |
| Country | Argentina |
| Sector | Public |
| PI Contribution | The Pirbright Institute currently coordinates a global network of fourteen International Reference Laboratories for FMD. |
| Collaborator Contribution | The Network of OIE/FAO FMD Reference Laboratories has been established with two principal goals: 1) To understand global virus distribution patterns and use these data to inform vaccine recommendations and 2) To harmonise and improve the quality of laboratory testing carried out by international and national reference laboratories. These activities require sharing and joint evaluation of surveillance information from laboratory diagnosis, serotyping, genetic characterisation and vaccine matching tests and harmonisation of standards for diagnostic procedures. |
| Impact | Outputs from the network provide vital information to international organisations involved in the control of FMD (such as OIE and FAO), as well as specific regional and national programmes to control FMD |
| Start Year | 2006 |
| Description | OIE/FAO Laboratory Network for FMD |
| Organisation | National Centre for Foreign Animal Diseases (NCFAD) |
| Country | Canada |
| Sector | Public |
| PI Contribution | The Pirbright Institute currently coordinates a global network of fourteen International Reference Laboratories for FMD. |
| Collaborator Contribution | The Network of OIE/FAO FMD Reference Laboratories has been established with two principal goals: 1) To understand global virus distribution patterns and use these data to inform vaccine recommendations and 2) To harmonise and improve the quality of laboratory testing carried out by international and national reference laboratories. These activities require sharing and joint evaluation of surveillance information from laboratory diagnosis, serotyping, genetic characterisation and vaccine matching tests and harmonisation of standards for diagnostic procedures. |
| Impact | Outputs from the network provide vital information to international organisations involved in the control of FMD (such as OIE and FAO), as well as specific regional and national programmes to control FMD |
| Start Year | 2006 |
| Description | OIE/FAO Laboratory Network for FMD |
| Organisation | Onderstepoort Veterinary Institute |
| Country | South Africa |
| Sector | Academic/University |
| PI Contribution | The Pirbright Institute currently coordinates a global network of fourteen International Reference Laboratories for FMD. |
| Collaborator Contribution | The Network of OIE/FAO FMD Reference Laboratories has been established with two principal goals: 1) To understand global virus distribution patterns and use these data to inform vaccine recommendations and 2) To harmonise and improve the quality of laboratory testing carried out by international and national reference laboratories. These activities require sharing and joint evaluation of surveillance information from laboratory diagnosis, serotyping, genetic characterisation and vaccine matching tests and harmonisation of standards for diagnostic procedures. |
| Impact | Outputs from the network provide vital information to international organisations involved in the control of FMD (such as OIE and FAO), as well as specific regional and national programmes to control FMD |
| Start Year | 2006 |
| Description | OIE/FAO Laboratory Network for FMD |
| Organisation | Pan American Foot-and-Mouth Disease Center (Panaftosa) |
| Country | Brazil |
| Sector | Charity/Non Profit |
| PI Contribution | The Pirbright Institute currently coordinates a global network of fourteen International Reference Laboratories for FMD. |
| Collaborator Contribution | The Network of OIE/FAO FMD Reference Laboratories has been established with two principal goals: 1) To understand global virus distribution patterns and use these data to inform vaccine recommendations and 2) To harmonise and improve the quality of laboratory testing carried out by international and national reference laboratories. These activities require sharing and joint evaluation of surveillance information from laboratory diagnosis, serotyping, genetic characterisation and vaccine matching tests and harmonisation of standards for diagnostic procedures. |
| Impact | Outputs from the network provide vital information to international organisations involved in the control of FMD (such as OIE and FAO), as well as specific regional and national programmes to control FMD |
| Start Year | 2006 |
| Description | OIE/FAO Laboratory Network for FMD |
| Organisation | Project Directorate on Foot and Mouth Disease |
| Country | India |
| Sector | Public |
| PI Contribution | The Pirbright Institute currently coordinates a global network of fourteen International Reference Laboratories for FMD. |
| Collaborator Contribution | The Network of OIE/FAO FMD Reference Laboratories has been established with two principal goals: 1) To understand global virus distribution patterns and use these data to inform vaccine recommendations and 2) To harmonise and improve the quality of laboratory testing carried out by international and national reference laboratories. These activities require sharing and joint evaluation of surveillance information from laboratory diagnosis, serotyping, genetic characterisation and vaccine matching tests and harmonisation of standards for diagnostic procedures. |
| Impact | Outputs from the network provide vital information to international organisations involved in the control of FMD (such as OIE and FAO), as well as specific regional and national programmes to control FMD |
| Start Year | 2006 |
| Description | OIE/FAO Laboratory Network for FMD |
| Organisation | U.S. Department of Agriculture USDA |
| Department | Agricultural Research Service |
| Country | United States |
| Sector | Public |
| PI Contribution | The Pirbright Institute currently coordinates a global network of fourteen International Reference Laboratories for FMD. |
| Collaborator Contribution | The Network of OIE/FAO FMD Reference Laboratories has been established with two principal goals: 1) To understand global virus distribution patterns and use these data to inform vaccine recommendations and 2) To harmonise and improve the quality of laboratory testing carried out by international and national reference laboratories. These activities require sharing and joint evaluation of surveillance information from laboratory diagnosis, serotyping, genetic characterisation and vaccine matching tests and harmonisation of standards for diagnostic procedures. |
| Impact | Outputs from the network provide vital information to international organisations involved in the control of FMD (such as OIE and FAO), as well as specific regional and national programmes to control FMD |
| Start Year | 2006 |
| Description | OIE/FAO Laboratory Network for FMD |
| Organisation | Veterinary and Agrochemical Research Centre |
| Country | Belgium |
| Sector | Public |
| PI Contribution | The Pirbright Institute currently coordinates a global network of fourteen International Reference Laboratories for FMD. |
| Collaborator Contribution | The Network of OIE/FAO FMD Reference Laboratories has been established with two principal goals: 1) To understand global virus distribution patterns and use these data to inform vaccine recommendations and 2) To harmonise and improve the quality of laboratory testing carried out by international and national reference laboratories. These activities require sharing and joint evaluation of surveillance information from laboratory diagnosis, serotyping, genetic characterisation and vaccine matching tests and harmonisation of standards for diagnostic procedures. |
| Impact | Outputs from the network provide vital information to international organisations involved in the control of FMD (such as OIE and FAO), as well as specific regional and national programmes to control FMD |
| Start Year | 2006 |
| Description | CEEZAD BSL-3 Training/Transboundary Animal Disease Summer Program - Manhattan, Kansas, USA |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | CEEZAD BSL-3 Training/Transboundary Animal Disease Summer Program Provides a Unique Opportunity for Future Veterinary Professionals Ten future veterinary professionals with an interest in transboundary disease research took part in a two-week training program conducted by the Center of Excellence for Emerging Zoonotic and Animal Diseases (CEEZAD) at Kansas State University in coordination with the Kansas State University Biosecurity Research Institute (BRI). The program involved one week of exposure to operations, safety techniques and lab principles of high-containment BSL-3 work at the Biosecurity Research Institute at Kansas State University followed by a second week of visits to institutions involved in the animal health industry and lectures. Students, representing 10 universities from around the USA, heard from prominent professionals in the area of zoonotic and transboundary disease research. The participants include students in veterinary medicine, doctoral students and post-DVM residents |
| Year(s) Of Engagement Activity | 2016 |
| URL | http://ceezad.org/news_events/ |
| Description | Invited Seminar - Transboundary Seminar Series |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Contributed a talk to the transboundary seminar series hosted by the Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD) at Kansas State University, USA. The title of the talk was "Who infected who? - the use of sequence data to reconstruct transmission pathways in FMD outbreaks" - and described insights from our work on the evolutionary biology of FMD. |
| Year(s) Of Engagement Activity | 2016 |
| Description | Invited Talk - 17th International Symposium of the World Association of Veterinary Laboratory Diagnosticians, Saskatoon, Canada, June 2015 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited Talk: King D. P., Valdazo-González B., Freimanis G., King D. J., Wright C. F., Di Nardo A., Wadsworth J., Soubeyrand S., Knowles N. J., Kim J. and Haydon D. T. The challenges of linking genetic and epidemiological datasets to reconstruct transmission trees for livestock viral diseases. OIE Symposium at the 17th International Symposium of the World Association of Veterinary Laboratory Diagnosticians, Saskatoon, Canada, June 2015. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Keynote presentation - 7th International Symposium on Emerging and re-emerging Pig Diseases, Kyoto, Japan |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented a talk outlining our research findings - entitled: "King D.P., Logan G., Freimanis G. L., Wright C. F., King D. J., Knowles N. J., Wadsworth J., Lasecka L., Bachanek-Bankowska K., Di Nardo A., Orton R. and Haydon D. T. Using sequence data to understand the epidemiology of foot-and-mouth disease" at the 7th International Symposium on Emerging and re-emerging Pig Diseases, Kyoto, Japan, June 2015. |
| Year(s) Of Engagement Activity | 2015 |
| URL | http://emerging2015.com |
| Description | Webinar - FMD in Indonesia |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited speaker: Molecular analyses and diagnosis of FMD. Webinar of foot-and-mouth disease: research and control. Organized by the National Research and Innovation Agency (BRIN), Indonesia, May 2022. |
| Year(s) Of Engagement Activity | 2022 |
| URL | https://www.youtube.com/watch?v=mAj4FPeT02Q&t=8626s |