TPM - Tailoring Plant Metabolism - Work package 1 (WP1) - High value lipids for health and industry
Lead Research Organisation:
Rothamsted Research
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
Plant seeds are a reservoir of energy-dense molecules, including oils enriched in triacylglycerols (TAGs) that serve as the primary storage form of fatty acids. TAGs and their component fatty acids not only provide a major dietary calorie source but also serve as a renewable source of hydrocarbons for biofuels and industrial applications. By understanding and exploiting the diversity of lipid biosynthetic pathways in nature, seeds can be used as a canvas for the design and tailoring of biochemical pathways to generate diverse nutritional and industrial oils not found in oilseed crops. WP1 will examine the spatiotemporal regulation of lipid synthesis, using a combination of transcriptomics, lipidomics, mass spec imaging, tracer studies and metabolic modelling. The interactions between endogenous metabolism and introduced biochemical pathways will be analysed using Camelina lines engineered to produce non-native, very long chain polyunsaturated fatty acids. The potential to exploit novel regulatory mechanisms (including nuclear RNA export and targeted protein degradation) will also be explored. Finally, genome editing and conventional genetic improvement will be employed to improve the Camelina germplasm used for engineering (the "chassis"). Together, these approaches will provide a knowledge base to facilitate predictive metabolic engineering of a range of high-value lipids, including polyunsaturated fatty acids with proven health and nutritional benefits, wax esters for the lubricant and cosmetics markets and structured TAGs with desirable properties.
Planned Impact
unavailable
Organisations
- Rothamsted Research (Lead Research Organisation)
- UNIVERSITY COLLEGE LONDON (Collaboration)
- Cargill (Collaboration)
- University of Nottingham (Collaboration)
- National Physical Laboratory (Collaboration)
- Protein Technologies Ltd (Collaboration)
- University of Cambridge (Collaboration)
- Instituto de la Grasa (Collaboration)
- Heidelberg University (Collaboration)
- Cargill Europe (Collaboration)
- University of York (Collaboration)
- French National Institute of Agricultural Research (Collaboration)
- Stockholm University (Collaboration)
- University of Bologna (Collaboration)
- JOHN INNES CENTRE (Collaboration)
- UNILEVER (Collaboration)
- Universidade de São Paulo (Collaboration)
- UNIVERSITY OF BATH (Collaboration)
- Swedish University of Agricultural Sciences (Collaboration)
- Austrian Institute of Technology (Collaboration)
- International Rice Research Institute (Collaboration)
- Elsoms Seeds (Collaboration)
- Julich Research Centre (Collaboration)
- UNIVERSITY OF LIVERPOOL (Collaboration)
- Ingenza Ltd (Collaboration)
- University of Nebraska-Lincoln (Collaboration)
- University of Southampton (Collaboration)
- Yield10 Bioscience (Collaboration)
- Commonwealth Scientific and Industrial Research Organisation (Collaboration)
- Camelina Company España SL (Collaboration)
- French Agricultural Research Centre for International Development (Collaboration)
- AAK AB (Collaboration)
- Punjab Aricultural University (Collaboration)
- University of Buenos Aires (Collaboration)
- University of Huelva (Collaboration)
- National Institute of Agronomy and Botany (NIAB) (Collaboration)
- Cardiff University (Collaboration)
- Pilot Pflanzenöltechnologie Magdeburg (Collaboration)
Publications
Haslam RP
(2017)
Green light for lipid fingerprinting.
in Biochimica et biophysica acta. Molecular and cell biology of lipids
Wintle BC
(2017)
A transatlantic perspective on 20 emerging issues in biological engineering.
in eLife
Luginbuehl LH
(2017)
Fatty acids in arbuscular mycorrhizal fungi are synthesized by the host plant.
in Science (New York, N.Y.)
Luginbuehl LH
(2017)
Fatty acids in arbuscular mycorrhizal fungi are synthesized by the host plant.
in Science (New York, N.Y.)
Sayanova O
(2017)
Modulation of lipid biosynthesis by stress in diatoms.
in Philosophical transactions of the Royal Society of London. Series B, Biological sciences
Min B
(2017)
Effects of Cultivar and Nitrogen Nutrition on the Lipid Composition of Wheat Flour.
in Journal of agricultural and food chemistry
Kong F
(2017)
Chlamydomonas carries out fatty acid ß-oxidation in ancestral peroxisomes using a bona fide acyl-CoA oxidase
in The Plant Journal
Vanhercke T
(2017)
Step changes in leaf oil accumulation via iterative metabolic engineering.
in Metabolic engineering
| Description | This ISPG WP aims to advance and exploit fundamental knowledge of plant metabolism, to expand the value chains of existing crops, predominantly focused on the niche crop, Camelina sativa. WP1.1 Understanding and manipulating the regulation of metabolism. Significant progress has been made with optimisation of spatiotemporal expression (WP1.1.1). To identify factors associated with accumulation of non-native omega-3 fatty acids (eicosapentaenoic and docosahexaenoic acid; EPA+DHA), tissue-specific metabolite imaging of Camelina sativa seeds was combined with spatial transcriptomic analysis, as previously reported. Novel distributions of metabolites in engineered omega-3 lines have now been confirmed by manual dissection and lipid analysis. Integrated 'omic data analysis identified novel candidate genes for manipulation of seed oil composition (WRI2, GPAT2, GPAT4 and PDAT), and seeds of multiple independent transgenic lines in wild type and transgenic backgrounds were screened (lipid profiling) to assess their effect on spatial and lipid class specific accumulation of novel Omega-3 fatty acids. Two additional regulatory mechanisms relevant to metabolic engineering were also explored. 1.1.2 RNA export and transgene stability: A panel of commonly used 3'-UTR sequences was assessed for efficacy in both single-gene and multi-gene constructs, as was the impact of multimerising terminators to restrict potential readthrough from adjacent expression units. Optimal promoter/5'-UTR and 3'-UTR/terminator combinations were identified by evaluating 20 different permutations in both the glasshouse and the field. In conjunction with the RNA-seq studies in 1.1.1, long-read and short-read whole genome sequence has been obtained for several benchmark transgenic lines (DHA1, EPA2015.8, ASX-2). Long-read de novo assemblies revealed complex T-DNA structures previously undetected by the Ion Torrent short-read technology. These data have significance not only for informing our understanding of transcript levels in these events but also for the pathway to regulatory approval as well as wider relevance to the plant sciences community. 1.1.3 Manipulating targeted protein degradation for crop improvement: two E3 ligases were demonstrated to have novel roles in plant immunity, the hypoxia response and control of suberin biosynthesis. In Year 6, the objective was (re)focused on understanding mechanisms controlling transgene-encoded protein expression, to align with 1.1.2 and 1.2.3. Label-free and isobaric labelling workflows for quantitative proteomic analysis of developing wild type and transgenic camelina seeds have been developed. Analysis of an elite line engineered to produce omega-3 fish oils revealed imperfect correlation between protein and transcript levels, indicating extensive post-transcriptional regulation of transgenes, and providing first insights into the impact of transgenesis on the seed proteome. The label-free datasets also enable design of absolute quantification approaches to be employed for future characterisation of transgenics and regulatory approval. WP1.2. Trials exploiting natural diversity in camelina to identify optimal germplasm for multi-gene engineering (1.2.1) were previously reported and this work is now being pursued further via uplift funding (H2020_UNTWIST). Current efforts are directed towards improving product delivery via crop engineering and mutagenesis with a focus on seed size/number (1.2.2) and oil yield (1.2.3). Ectopic expression in Arabidopsis of six brassinosteroid biosynthesis or signalling genes has now revealed the potential of each to increase seed yield (1.2.2). Further optimisation of the promoter driving this expression has been undertaken and is being tested in Arabidopsis. Homozygous lines in camelina for the original constructs are currently being obtained for rigorous assessment of the yield trait and may provide a promising target to introduce into our benchmark omega-3 lines. A first attempt at introducing one of these genes in oilseed rape has been completed and is being progressed to the next generation. Optimising oil yield (1.2.3) has comprised several new, complementary approaches to chassis improvement: (i) Endogenous camelina seed oil assembly pathways were targeted using a multiplex CRISPR approach in wild type and our benchmark camelina line (DHA1; collaboration with Prof Fauré, Paris AgroTech). Primary screening of multiple lines observed changes in seed fatty acid profiles, including increased EPA+DHA and total oil content. Screening of pooled T4 seed has confirmed the seed oil phenotype, and PacBio sequencing has allowed the correlation of acyltransferase gene edits with increased accumulation of novel fatty acids. (ii) Proof of concept has been obtained, and published in year 6, for using large CRISPR/Cas9 deletions to create gain of function phenotypes by bringing target genes under the control of the promoters of other upstream genes ("promoter pinching"). Putting Arabidopsis DGAT2 under the control of its upstream gene, which is ~10 times more strongly expressed, increased leaf oil content. (iii) The synthesis of non-native fatty acids in transgenic plants often has a negative impact on oil yield. Lipidomic analysis of developing seed from wild type and the benchmark DHA1 line indicated that this penalty is established early in seed/embryo development. Analysis by qPCR of the transgenes and a subset of endogenous lipid regulatory genes revealed insights into the temporal expression of these transgenes and their metabolic integration with endogenous metabolism. A series of time course RNA-seq studies sampled during seed development (oil accumulation phase) established a detailed transcriptome time series allowing the characterisation of the developmental programme of seed oil assembly in WT and benchmark omega-3 lines. Candidate genes have been identified from this work including some involved in fundamental lipid bio-synthesis. (iv) Finally, we have created multi-mutants in Medicago truncatula and Hordeum vulgare with elevated oil content in seeds and leaves. We have isolated dominant mutants in wheat with elevated oil content in seeds and leaves, in one case, identifying the causal mutation. This mutation could be used as a non-GM approach in grasses to increase livestock productivity and reduce enteric methane emissions of cattle and sheep. These discoveries will be pursued via uplift funding. Building on the significant outputs of WP1.1 and 1.2, iterative metabolic engineering has confirmed the utility of camelina for delivery of high-value polyunsaturated fatty acids (PUFAs; 1.3.1) Two sets of constructs were generated for the further enhancement of EPA+DHA levels and an oil profile specifically tailored to the needs of aquaculture. The first panel comprised seven iterations based around the successful DHA1 prototype which has been extensively field trialled both in UK and N. America. The aim of these constructs was to generate a range of oils with different levels of useful fatty acids. The second panel of 26 constructs was built with the objective of validating new activities for the synthesis of EPA+DHA in transgenic camelina, leading to new IP. Leads from over 1000 individual events were taken forward for field evaluation in 2021 and 2022. GM lines were released in these GM field trial, as were GM+GE stacks for enhanced accumulation of EPA+DHA (via gene editing of the competing FAE1 pathway), and a triple stack line containing transgenes for EPA+DHA, astaxanthin and herbicide tolerance. Collectively, these data guide rational design of improved contructs for optimal accumulation of target fatty acids. Further iterations for metabolic engineering of non-methylene-interrupted PUFAs with pharmaceutical potential have been conducted and T3 seed of camelina lines producing sciadonic acid (a fatty acid with anti-inflammatory, triglyceride-lowering and satiety-modulating properties) were sown in the 2021 field trail. In addition to fine-tuning the PUFA pathway, we have made significant progress in multigene metabolic engineering of two further groups of high-value lipids, particularly structured TAGs for better infant nutrition (1.3.2). Proof of concept in oilseeds has previously been reported and the technology refined to make the key target molecule 1,3-olein-2-palmitin. In response to industry feedback focus has transitioned to oleaginous yeast as a convenient platform for production by microbial fermentation. A patent application covering plant and microbial platforms has been filed. Camelina lines producing higher levels of wax esters as a source of renewable high value biolubricants and feedstocks (1.3.3) have been generated and will be tested in the future with material produced used to facilitate further refinement of industrial extraction methods. |
| Exploitation Route | Too early to say at this stage, though it is important to note that all objectives have so far been met and we are on track to deliver those associated with the final year of this programme. Since these outputs map onto our Impact Plan, there are good prospects for realizing tangible outcomes and impact from this research. |
| Sectors | Agriculture Food and Drink Healthcare Manufacturing including Industrial Biotechology Pharmaceuticals and Medical Biotechnology |
| Description | Contribution to the on-going debate as how to regulate GM and genome edited crops in Europe and post-Brexit Europe. This involves discussions with DEFRA representatives close to the Minister responsible for this area. Notably, the ISPG has played a leading role in pioneering increasingly sophisticated GM field trials, and in public dialogue around GM and GE. Examples from this project have been used as background briefing material in the recently announced DEFRA public consultation on the post-EU regulation of GE. Importantly, the precedent set by the GE field trial carried out as part of this ISPG in 2018 effectively defined the case-law for the newly announced classification in England of GE as being non-GMO. See opinion from ACRE: https://assets.publishing.service.gov.uk/government/uploads/system/uploads/attachment_data/file/800593/gmo-camelina-oleic-acre-advice.pdf.pdf |
| First Year Of Impact | 2017 |
| Sector | Agriculture, Food and Drink |
| Impact Types | Economic Policy & public services |
| Description | Presentation at House of Lord Select Committee |
| Geographic Reach | Local/Municipal/Regional |
| Policy Influence Type | Contribution to a national consultation/review |
| Description | Ted talk on plant biotechnology and crop genetic improvement: Department for International Development (DFID), Agriculture Research Team. |
| Geographic Reach | Multiple continents/international |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Description | 21ROMITIGATIONFUND Rothamsted |
| Amount | £924,000 (GBP) |
| Funding ID | BB/W510543/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 08/2021 |
| End | 03/2022 |
| Description | Application for a new Confocal Microscope |
| Amount | £721,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 06/2021 |
| End | 03/2022 |
| Description | Application for a new JEOL JEM-2100Plus Transmission Electron Microscope |
| Amount | £635,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 08/2019 |
| End | 03/2020 |
| Description | Application for a new Zeiss Axio Imager.Z2 High Resolution Light Microscope |
| Amount | £97,200 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 08/2019 |
| End | 03/2020 |
| Description | Application for new Quorum SEM cryo sample preparation systems |
| Amount | £306,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2020 |
| End | 03/2021 |
| Description | BBSRC sLoLa Brassica Rapeseed And Vegetable Optimisation |
| Amount | £3,514,227 (GBP) |
| Funding ID | BB/P003095/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2017 |
| Description | BR2CSFB - A toolkit for breeding resistance to adult and larval herbivory by the cabbage stem flea beetle |
| Amount | £408,513 (GBP) |
| Funding ID | BB/V015621/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2021 |
| End | 09/2025 |
| Description | Brassica Rapeseed And Vegetable Optimisation |
| Amount | £3,514,227 (GBP) |
| Funding ID | BB/P003095/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2017 |
| End | 12/2022 |
| Description | COVID-19 Grant Extension Allocation |
| Amount | £77,648 (GBP) |
| Organisation | United Kingdom Research and Innovation |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2021 |
| End | 09/2021 |
| Description | Development of non-glaucous Brassica napus lines by genome editing for enhancing pathogen resistance and reducing chemical sprays. |
| Amount | 4,119,484 kr (SEK) |
| Organisation | Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning (FORMAS) |
| Sector | Private |
| Country | Sweden |
| Start | 03/2018 |
| End | 02/2022 |
| Description | FTMA4: Training and mobility to support multi-omic analysis |
| Amount | £27,392 (GBP) |
| Funding ID | BB/X017877/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2023 |
| End | 03/2023 |
| Description | Functional Food and Pharma Ingredients from the Physical Dismantling of Oilseed Rape Oleosomes |
| Amount | £98,212 (GBP) |
| Funding ID | BB/R506278/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2018 |
| End | 02/2022 |
| Description | GCRF Agri Tech Seeding Award |
| Amount | £15,546 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2021 |
| End | 09/2021 |
| Description | GCRF AgriFood Africa Innovation Awards Round 3 (New genomic resources for breeding better direct seeded rice in Ghana) |
| Amount | £29,828 (GBP) |
| Organisation | Innovate UK |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2022 |
| End | 04/2023 |
| Description | Genetic improvement of rice seed vigour for dry direct-seeded conditions |
| Amount | £520,445 (GBP) |
| Funding ID | BB/P023428/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2017 |
| End | 04/2019 |
| Description | Genetic improvement of seed vigour in African rice for direct seeding |
| Amount | £19,771 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2021 |
| End | 09/2021 |
| Description | Genomics-led improvement of biotic and abiotic stress tolerance in mustard rape for economic and environmental sustainability |
| Amount | £1,764,019 (GBP) |
| Funding ID | BB/R019819/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 08/2018 |
| End | 08/2022 |
| Description | Global Challenges Research Fund - Impact Acceleration Award Extension 2017 - 'Development and validation of new open-source phenotyping platforms for analysis of rice seedling vigour' |
| Amount | £5,740 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 12/2017 |
| End | 02/2018 |
| Description | International Exchanges 2018 Cost Share (Argentina, France and Russia only) |
| Amount | £10,670 (GBP) |
| Funding ID | IEC\R2\181074 |
| Organisation | The Royal Society |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 12/2018 |
| End | 11/2020 |
| Description | International Science Fund 2 Developing a North American partnership for bigger and better Brassicaceae. |
| Amount | £46,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 11/2022 |
| End | 03/2023 |
| Description | LC-MS system for proteomics |
| Amount | £2,082,587 (GBP) |
| Funding ID | IGP22-035 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2022 |
| End | 09/2024 |
| Description | Mass Spectrometry Based Lipidomics and Metabolomics to Drive Bioscience Discovery |
| Amount | £750,000 (GBP) |
| Funding ID | BB/S019588/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | |
| End | 04/2022 |
| Description | Mass Spectrometry Based Lipidomics and Metabolomics to Drive Bioscience Discovery |
| Amount | £750,000 (GBP) |
| Funding ID | BB/S019588/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | |
| End | 03/2022 |
| Description | New varieties of direct seeded rice for farmers in Lower Middle Income Countries |
| Amount | £602,712 (GBP) |
| Funding ID | EP/T015551/1 |
| Organisation | Engineering and Physical Sciences Research Council (EPSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2019 |
| End | 03/2021 |
| Description | Novel Omega-3 Sources in Feeds and Impacts on Salmon Health |
| Amount | £240,067 (GBP) |
| Funding ID | BB/S006400/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2019 |
| End | 12/2022 |
| Description | Nutrition and abiotic stress tolerance in Tomato |
| Amount | £25,000 (GBP) |
| Organisation | Innovate UK |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2018 |
| End | 03/2019 |
| Description | Oilseed Rape Genetic Improvement Network (OREGIN) phase 5 |
| Amount | £850,000 (GBP) |
| Organisation | Department For Environment, Food And Rural Affairs (DEFRA) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2018 |
| End | 03/2023 |
| Description | Production of the lipid-soluble antioxidant Canolol in cruciferous oilseeds - BBSRC HVCfP NIBB PoC |
| Amount | £39,026 (GBP) |
| Organisation | University of York |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 03/2017 |
| End | 01/2018 |
| Description | Provision of a replacement automated TD-NMR system for seed oil and moisture measurements |
| Amount | £246,363 (GBP) |
| Funding ID | IGP22-038 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 12/2022 |
| End | 04/2023 |
| Description | Provision of a replacement ultracentrifuge and rotors for fundamental biochemical studies at Rothamsted |
| Amount | £144,284 (GBP) |
| Funding ID | IGP22-015 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2022 |
| End | 03/2023 |
| Description | RCUK-CIAT Newton Fund: Exploiting biodiversity in Brachiaria/Panicum tropical forage grasses using genetics to improve livelihoods and sustainability |
| Amount | £150,692 (GBP) |
| Funding ID | BB/R022828/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2018 |
| End | 03/2019 |
| Description | Rutherford Fund |
| Amount | £12,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 06/2018 |
| End | 12/2018 |
| Description | Rutherford fellowship - flexible talent mobility award |
| Amount | £11,000 (GBP) |
| Funding ID | BB/R506503/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 06/2018 |
| End | 12/2018 |
| Description | SW Bio DTP |
| Amount | £100,000 (GBP) |
| Organisation | University of Bath |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 08/2022 |
| End | 09/2026 |
| Description | Science Catalyst Innovation Award Understanding plant-pathogen interactions in wheat under elevated temperatures. |
| Amount | £75,000 (GBP) |
| Organisation | Rothamsted Research |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 08/2021 |
| End | 09/2022 |
| Description | Seeding Catalyst Awards |
| Amount | £10,000 (GBP) |
| Funding ID | BB/SCA/Rothamsted/17 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 11/2017 |
| End | 02/2018 |
| Description | Sustainable sources of omega-3 fish oils |
| Amount | £150,000 (GBP) |
| Organisation | AstraZeneca |
| Sector | Private |
| Country | United Kingdom |
| Start | 03/2019 |
| End | 12/2021 |
| Description | UK-Brazil partnership BBSRC/FAPESP. Validation of Arabidopsis thaliana as a suitable model system for investigating the dual protein-lipid antigenic response required for sensitisation to Brazil Nut Ber e 1 |
| Amount | £18,000 (GBP) |
| Funding ID | BBS/OS/NW/000001 |
| Organisation | Newton Fund |
| Sector | Public |
| Country | United Kingdom |
| Start | 06/2018 |
| End | 12/2018 |
| Description | UK-China Centre for the Sustainable Intensification of Agriculture (CSIA) |
| Amount | £500,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2018 |
| End | 03/2019 |
| Description | UNTWIST (Uncover and promote tolerance to temperature and water stress in Camelina sativa) |
| Amount | £5,000,000 (GBP) |
| Funding ID | SFS-30-2018-2019-2020 Agri |
| Organisation | European Union |
| Sector | Public |
| Country | European Union (EU) |
| Start | 08/2020 |
| End | 09/2025 |
| Description | Utility of a plant hydrolase as an industrial biocatalyst - |
| Amount | £15,929 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2018 |
| End | 09/2018 |
| Description | Utility of a plant hydrolase as an industrial biocatalyst - BBSRC HVCfP NIBB PoC |
| Amount | £15,929 (GBP) |
| Organisation | University of York |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 03/2018 |
| End | 09/2018 |
| Description | What determines protein abundance in plants? |
| Amount | £3,354,456 (GBP) |
| Funding ID | BB/T002182/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2020 |
| End | 10/2025 |
| Description | iCURE - UltraPUFAs |
| Amount | £30,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 05/2022 |
| End | 09/2022 |
| Description | iCURE-LLP |
| Amount | £4,500 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 02/2022 |
| End | 05/2022 |
| Title | A bioinformatics pipeline for the analysis of camelina multiplex CRISPR/Cas9 lines |
| Description | An online tool for the analysis of gene sequencing (PacBio) to identify the location multiplex CRISPR/Cas9 edits in camelina gene homologs. Developed in collaboration with Prof. Jean-Denis Faure (INRAe, France). |
| Type Of Material | Improvements to research infrastructure |
| Year Produced | 2022 |
| Provided To Others? | No |
| Impact | The identification of 'gain of function' camelina lines with increased novel omega-3 fatty acid content and total seed oil. Specifically lines edited in acyltransferase (seed oil production) activity. |
| Title | A live imaging system of measuring etiolated seedling growth |
| Description | A live imaging system of measuring etiolated seedling growth |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | We have developed a time-lapse image capture system for measuring the rate of rice seed germination and seedling growth in the dark. The purpose of this technology is to quantify phenotypic variation in seed/seedling vigour in order to assist in genetic improvement for rice for direct seeding. The system can also be applied to other crops in which early vigour is important. |
| Title | A live imaging system to measure germination |
| Description | We have refined a time-lapse image capture system for measuring the rate of rice seed germination in the dark. The system can also be applied to other crops in which early vigour is important. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2020 |
| Provided To Others? | No |
| Impact | We are liaising with collaborators to test the system with various crop species. |
| Title | Click-chemistry for in vivo labelling of membrane lipids |
| Description | Click-chemistry for in vivo labelling of membrane lipids |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2019 |
| Provided To Others? | No |
| Impact | Uptake of propargylcholine, followed by click chemistry with fluorescein or Alexa Fluor 594 azide was used to visualize choline phospholipids in cells of root, leaf, stem, silique and seed tissues from Camelina. PC tunover and accumulation can be further determine by mass spectrometry. |
| Title | GWAS and gene discovery pipeline |
| Description | GWAS and gene discovery pipeline |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | Facilitate rapid and accurate discovery of gene of interest controlling various agronomic traits |
| Title | Germplasm Multiplex CRISPR/Cas9 Gene Edited Camelina |
| Description | Multiplex CRISPR/Cas9 Gene Edited Camelina |
| Type Of Material | Cell line |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | Gene edited germplasm provides a resource for engineering novel seed fatty acid traits |
| Title | High resolution accurate mass lipidomics for plants |
| Description | Investment by Rothamsted in a accurate mass Thermo Q Exactive Plus MS has updated and extended analytical capacity. A lipidomics workflow for the Q Exactive has been developed, incorporating an MTBE optimised sample extraction and UPLC method, the Q Exactive Instrument Method, LipidSearch software workflow and lipidomics quality control. We have optimised for identification, alignment and quantitation of all plant lipid species. We have deployed the new instrument to investigate tissue-specific novel fatty acid assembly into seed oil. See: Haslam, R.P. and Feussner, I. (2017) Green light for lipid fingerprinting. BBA Molecular and Cell Biology of Lipids 1862(8):782-785. Commissioning the new instrumentation has provided the ISPG with increased analytical capacity, efficiency, responsiveness and flexibility. Moreover, it has enabled the development of a new workflow, lipid fingerprinting, that integrates the advantages of shotgun lipidomics (quantitative data) with LC-MS-based strategies (higher resolution and/or coverage). |
| Type Of Material | Improvements to research infrastructure |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | The enhanced analytical capability provide by this instrumentation has enabled the development of a new workflow, lipid fingerprinting, that integrates the advantages of shotgun lipidomics (quantitative data) with LC-MS-based strategies (higher resolution and/or coverage). Collectively, the instrumentation and associated workflows enable the development of new knowledge-based strategies to redesign lipid pathways for the sustainable production of high value products. |
| Title | High throughput HPLC-ESI-MS/MS profilling method for analysis of free and esterified sterols in biological samples |
| Description | We have developed a high throughput HPLC/ELSD-ESI-MS/MS method for the compositional and content analysis of seed sterols and steryl esters in a B. napus diversity collection (over 400 accessions representing the ASSYST panel). 1600 samples analysed. This methods expanded the analytical capability of the state of the art lipidomic platform available at Rothamsted Research. |
| Type Of Material | Improvements to research infrastructure |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | This methods has been extented to include detectection of wax esters as well as sterol esters in seed oil samples. This allowed the accurate compositional analysis of the ester fraction in commercial sunflower oil as well as in mutant varieties. This work may have implications for vegetable oil treatment and authentication allowing both the reduction of oil turbidity at room temperature (wax esters) and the detection of fraudulent mixture of high value oils with cheaper and lower quality oils (steryl esters markers). This work was submitted to food Chemistry and is curently under review: New insights in the composition of Wax and Sterol Esters in common and mutant sunflower oils revealed by ESI-MS/MS. Richard Broughton, Noemi Ruíz-Lopez, Enrique Martínez-Force, Rafael Garcés, Fred Beaudoin* and Joaquín J. Salas* (2018). |
| Title | Tandem fluorescent timers for protein lifetime measurement in plants |
| Description | A tandem fluorescent protein timer (tFT) is a fusion of two different fluorescent proteins with distinct fluorophore maturation kinetics, which enable protein age to be estimated from the ratio of fluorescence intensities of the two fluorescent proteins. We have adopted this technology (originally developed in yeast) for use in plants. tFTs expressed in transient systems enable efficient screens for relative protein lifetime, useful for testing the effects of mutations and different genetic backgrounds on protein stability. Stably expressed tFTs can be used to study native protein dynamics with high temporal resolution in response to exogenous or endogenous stimuli. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | The method has only just been published so it is too early to judge impact but conference presentations have led to other researchers adopting the method and we are aware of at least one study submitted for publication. |
| Title | Additional file 2 of Beyond the one-way ANOVA for â omics data |
| Description | Lipidomics model assessment. Table containing the overall assessment of statistical significance and the model fitted for each lipid under both RFM and MRF. (PDF 8 kb) |
| Type Of Material | Database/Collection of data |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| URL | https://springernature.figshare.com/articles/Additional_file_2_of_Beyond_the_one-way_ANOVA_for_omics... |
| Title | Additional file 2 of Beyond the one-way ANOVA for â omics data |
| Description | Lipidomics model assessment. Table containing the overall assessment of statistical significance and the model fitted for each lipid under both RFM and MRF. (PDF 8 kb) |
| Type Of Material | Database/Collection of data |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| URL | https://springernature.figshare.com/articles/Additional_file_2_of_Beyond_the_one-way_ANOVA_for_omics... |
| Title | Analysis of SNPs |
| Description | We created a semi automatic way of generating boxplots for SNPs highlighted by associative transcriptome analysis to take into account the sequence of the genes and how the difference in the nucleotide was affecting the trait. |
| Type Of Material | Data analysis technique |
| Year Produced | 2020 |
| Provided To Others? | No |
| Impact | Will be shared with collaborator. |
| Title | Annotated patches of whole oilseed rape (Brassica napus) plant images created using the MapReader pipeline |
| Description | Background and Dataset Creation: Patches derived from whole images of oilseed rape (Brassica napus) plants from the 'Collection of side view and top view RGB images of Brassica napus from a large scale, high throughput experiment' dataset and their associated annotations, which were used to train, validate and test patch classification models as described in the following paper: Corcoran, E., Hosseini, K., Siles, L., Kurup, S., and Ahnert, S. 2024. 'Automated dynamic phenotyping of whole oilseed rape (Brassica napus) plants from images collected under controlled conditions', Frontiers in Plant Science (under review). Patches were created and annotated using the MapReader pipeline. Please see: Kasra Hosseini, Daniel C. S. Wilson, Kaspar Beelen, and Katherine McDonough. 2022. MapReader: a computer vision pipeline for the semantic exploration of maps at scale. In Proceedings of the 6th ACM SIGSPATIAL International Workshop on Geospatial Humanities (GeoHumanities '22). Association for Computing Machinery, New York, NY, USA, 8-19. https://doi.org/10.1145/3557919.3565812 Kasra Hosseini, Rosie Wood, Andy Smith, Katie McDonough, Daniel C.S. Wilson, Christina Last, Kalle Westerling, and Evangeline Mae Corcoran. "Living-with-machines/mapreader: End of Lwm". Zenodo, July 27, 2023. https://doi.org/10.5281/zenodo.8189653. File structure: Annotations The 'annotations_six_label_sv_5.zip' folder contains annotations for the entire patch dataset in .csv format, these files have two columns 'image_id', 'label' in which: 'image_id' = the path to each image patch 'label' = the label assigned to each patch by the annotator indicated which part of the plant the patch primarily contained, or if it was part of the background. Labels: '0' = non-plant background, '1' = open flower, '2' = flower bud, '3' = leaf, '4' = greed pod containing seed, '5' = branch. Patches The 'b_napus_patch_data.zip' folder contains all patches in csv format. Each file is named in a consistent format e.g. "patch-1580-330-1590-340-#2018-07-06_00_VIS_sv_000-0-0-0.png#.PNG" where '1580-330-1590-340' are the x and y coordinates of the patch boundary and '#2018-07-06_00_VIS_sv_000-0-0-0.png#' indicates the image in the 'Collection of side view and top view RGB images of Brassica napus from a large scale, high throughput experiment' from which the patch was derived. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2024 |
| Provided To Others? | Yes |
| URL | https://zenodo.org/doi/10.5281/zenodo.12721800 |
| Title | Annotated patches of whole oilseed rape (Brassica napus) plant images created using the MapReader pipeline |
| Description | Background and Dataset Creation: Patches derived from whole images of oilseed rape (Brassica napus) plants from the 'Collection of side view and top view RGB images of Brassica napus from a large scale, high throughput experiment' dataset and their associated annotations, which were used to train, validate and test patch classification models as described in the following paper: Corcoran, E., Hosseini, K., Siles, L., Kurup, S., and Ahnert, S. 2024. 'Automated dynamic phenotyping of whole oilseed rape (Brassica napus) plants from images collected under controlled conditions', Frontiers in Plant Science (under review). Patches were created and annotated using the MapReader pipeline. Please see: Kasra Hosseini, Daniel C. S. Wilson, Kaspar Beelen, and Katherine McDonough. 2022. MapReader: a computer vision pipeline for the semantic exploration of maps at scale. In Proceedings of the 6th ACM SIGSPATIAL International Workshop on Geospatial Humanities (GeoHumanities '22). Association for Computing Machinery, New York, NY, USA, 8-19. https://doi.org/10.1145/3557919.3565812 Kasra Hosseini, Rosie Wood, Andy Smith, Katie McDonough, Daniel C.S. Wilson, Christina Last, Kalle Westerling, and Evangeline Mae Corcoran. "Living-with-machines/mapreader: End of Lwm". Zenodo, July 27, 2023. https://doi.org/10.5281/zenodo.8189653. File structure: Annotations The 'annotations_six_label_sv_5.zip' folder contains annotations for the entire patch dataset in .csv format, these files have two columns 'image_id', 'label' in which: 'image_id' = the path to each image patch 'label' = the label assigned to each patch by the annotator indicated which part of the plant the patch primarily contained, or if it was part of the background. Labels: '0' = non-plant background, '1' = open flower, '2' = flower bud, '3' = leaf, '4' = greed pod containing seed, '5' = branch. Patches The 'b_napus_patch_data.zip' folder contains all patches in csv format. Each file is named in a consistent format e.g. "patch-1580-330-1590-340-#2018-07-06_00_VIS_sv_000-0-0-0.png#.PNG" where '1580-330-1590-340' are the x and y coordinates of the patch boundary and '#2018-07-06_00_VIS_sv_000-0-0-0.png#' indicates the image in the 'Collection of side view and top view RGB images of Brassica napus from a large scale, high throughput experiment' from which the patch was derived. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2024 |
| Provided To Others? | Yes |
| URL | https://zenodo.org/doi/10.5281/zenodo.12721801 |
| Title | Macro for automatic tracing of seed/ovule size |
| Description | A Image J macro was created will allows for automatic analyses of image data to extract seed/ovule size data. |
| Type Of Material | Data analysis technique |
| Year Produced | 2020 |
| Provided To Others? | No |
| Impact | Shared with collaborators. |
| Title | N-terminal peptide dataset |
| Description | Dataset of N-terminal peptides from Arabidopsis seedlings, with relative quantitation |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | This dataset underpins a publication and has also been mined by colleagues for other purposes (for example, the identification of N-terminally acetylated proteins). The data has also been used in a major NSF funded project, which aims to re-process a large number of Arabidopsis PRIDE submissions deposited in ProteomeXchange. |
| URL | http://proteomecentral.proteomexchange.org/cgi/GetDataset?ID=PXD006450 |
| Title | Quantitative proteome analysis of the Arabidopsis thaliana N-degron pathway E3 ligase mutant, prt1-1. |
| Description | Mass spectrometry proteomics data set with relative quantification. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | This dataset underpins a publication and has also been mined by colleagues for other purposes (for example, comparison with data from other tissues and different N-degron pathway mutants). The data has also been used in a major NSF funded project, which aims to re-process a large number of Arabidopsis PRIDE submissions deposited in ProteomeXchange. |
| URL | https://www.ebi.ac.uk/pride/archive/projects/PXD014238 |
| Title | Quantitative proteomics analysis of Arabidopsis roots treated with ethylene. |
| Description | Quantitative proteomics analysis of Arabidopsis roots treated with ethylene. MS proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PXD 031138 |
| Type Of Material | Database/Collection of data |
| Year Produced | 2020 |
| Provided To Others? | No |
| Impact | The data are used in paper currently at the pre-print stage |
| Title | Quantitative proteomics dataset for WT and GM Camelina seeds |
| Description | Quantitative proteomics analysis of WT and GM Camelina sativa seeds at 25 days after pollination. Proteins were quantified using TMT isobaric labelling and tandem MS. The data have been deposited in the PRIDE database, accession number, PXD028383 and are embargoed until publication. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2021 |
| Provided To Others? | No |
| Impact | In combination with transcriptome analysis, this data set reveals a lack of correlation between transcript and protein levels for transgene-encoded proteins, indicating post-transcriptional regulation. It also reveals the impact of transgenesis on the seed proteome. |
| Title | Transcriptome dataset |
| Description | We have generated a Brassica tissue specific transcriptome data set across 5 genotypes, 18 distinct tissues and several developmental stage. |
| Type Of Material | Data analysis technique |
| Year Produced | 2020 |
| Provided To Others? | No |
| Impact | Will allow the identification of key regulators for yield traits. Will be available once published. |
| Description | Analysis of the Wax and Steryl Ester content in vegetable oils. Implications for oil processing, authentification and commercialisation. |
| Organisation | Instituto de la Grasa |
| Country | Spain |
| Sector | Private |
| PI Contribution | Lipidomic profiling of oil minor components (wax and lipid soluble bioactives) GC-FID, GC-MS, ESI-MS/MS |
| Collaborator Contribution | Oil crops cultivation and harvesting Oil extraction, testing of biophysical properties |
| Impact | one research article in Food sciences |
| Start Year | 2017 |
| Description | Collaboration with Camelina Company |
| Organisation | Camelina Company España SL |
| Country | Spain |
| Sector | Private |
| PI Contribution | Camelina abiotic stress tolerance |
| Collaborator Contribution | Germplasm, field trials |
| Impact | On-going work |
| Start Year | 2020 |
| Description | Collaboration with Christophe PERIN |
| Organisation | French Agricultural Research Centre for International Development |
| Country | France |
| Sector | Private |
| PI Contribution | discussion regarding research, possible collaborations |
| Collaborator Contribution | new tools for analysing transcriptome data |
| Impact | on-going work |
| Start Year | 2021 |
| Description | Collaboration with DOE (USA) funded Bigger and Better Brassicaceae consortium |
| Organisation | University of Nebraska-Lincoln |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Collaborative discussions on the application of biotechnology for camelina crop improvement. |
| Collaborator Contribution | Collaborative discussions on the application of biotechnology for camelina crop improvement. |
| Impact | A memorandum of understanding is currently being negotiated between RREs and the University of Nebraska (USA). |
| Start Year | 2022 |
| Description | Collaboration with DR Tara Hessa |
| Organisation | Stockholm University |
| Country | Sweden |
| Sector | Academic/University |
| PI Contribution | Lipidomic profiling of mouse retina |
| Collaborator Contribution | Visting worker for lipid extraction |
| Impact | Ongoing work |
| Start Year | 2019 |
| Description | Collaboration with Dr Arvind Kumar |
| Organisation | International Rice Research Institute |
| Country | Philippines |
| Sector | Charity/Non Profit |
| PI Contribution | Rice seedlings phenotyping, GWAS for direct seeding |
| Collaborator Contribution | To be entered |
| Impact | Ongoing work |
| Start Year | 2017 |
| Description | Collaboration with Dr Bjorn Usadel |
| Organisation | Julich Research Centre |
| Department | Institute of Bio and Geosciences 1 (IBG-1) |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | Camelina abiotic Stress tolerance |
| Collaborator Contribution | Genome sequencing |
| Impact | On-going work |
| Start Year | 2020 |
| Description | Collaboration with Dr Claudia Jonack |
| Organisation | Austrian Institute of Technology |
| Country | Austria |
| Sector | Private |
| PI Contribution | Camelina abiotic Stress tolerance |
| Collaborator Contribution | Germplasm, antioxidant analysis |
| Impact | On-going work |
| Start Year | 2020 |
| Description | Collaboration with Dr Federica Zanetti |
| Organisation | University of Bologna |
| Country | Italy |
| Sector | Academic/University |
| PI Contribution | Camelina abiotic stress tolerance |
| Collaborator Contribution | Germplasm, field trials |
| Impact | on-going work |
| Start Year | 2020 |
| Description | Collaboration with Dr Graham Burdge |
| Organisation | University of Southampton |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Lipidomic profiling of human serum lipids |
| Collaborator Contribution | Serum lipid extracts from human trial testing fish oil and O3 camelina oil |
| Impact | Ongoing work |
| Start Year | 2019 |
| Description | Collaboration with Dr Kenneth Tong |
| Organisation | University College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Re-engineering the biomechanics of seedling emergence to enhance crop performance (RICE+) |
| Collaborator Contribution | To be entered |
| Impact | Ongoing work |
| Start Year | 2018 |
| Description | Collaboration with Dr Kim Olofsson |
| Organisation | AAK AB |
| Country | Sweden |
| Sector | Private |
| PI Contribution | Sample provision |
| Collaborator Contribution | Lipidomic analysis of samples |
| Impact | on-going work |
| Start Year | 2020 |
| Description | Collaboration with Dr Michiel Matthijs |
| Organisation | Cargill Europe |
| Country | Belgium |
| Sector | Private |
| PI Contribution | Strain provision |
| Collaborator Contribution | Fermentation testing |
| Impact | On-going work |
| Start Year | 2020 |
| Description | Collaboration with Dr Nitika Sandhu |
| Organisation | Punjab Aricultural University |
| Country | India |
| Sector | Academic/University |
| PI Contribution | Rice seedlings phenotyping, GWAS for direct seeding |
| Collaborator Contribution | To be entered |
| Impact | Ongoing work |
| Start Year | 2017 |
| Description | Collaboration with Josh Bilton |
| Organisation | University of Southampton |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Lipidomics |
| Collaborator Contribution | Biological samples |
| Impact | on-going work |
| Start Year | 2020 |
| Description | Collaboration with Prof Cathie Martin JIC, |
| Organisation | John Innes Centre |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Lipidomics, plant growth |
| Collaborator Contribution | Germplasm Analysis |
| Impact | On-going work |
| Start Year | 2020 |
| Description | Collaboration with Prof Ed Cahoon, UNL |
| Organisation | University of Nebraska-Lincoln |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Metabolic engineering of camelina for high value lipids and chemicals |
| Collaborator Contribution | Provision of US-based GM field trial capability |
| Impact | Still in progress |
| Start Year | 2017 |
| Description | Collaboration with Prof Fernando Carrari |
| Organisation | University of Buenos Aires |
| Country | Argentina |
| Sector | Academic/University |
| PI Contribution | Lipidomic profiling of Tomato variation in response to heat stress |
| Collaborator Contribution | To be entered |
| Impact | Ongoing work |
| Start Year | 2019 |
| Description | Collaboration with Prof John Harwood |
| Organisation | Cardiff University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Role of the lipidome on cold stress tolerance |
| Collaborator Contribution | Tracking expression of transcriptin factors |
| Impact | Ongoing work |
| Start Year | 2019 |
| Description | Collaboration with Prof Kathryn Lilley: label-free quantification of Camelina seed proteins |
| Organisation | University of Cambridge |
| Department | Cambridge Centre for Proteomics |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Our team provided the biological question, grew plant material and extracted proteins. Data were discussed and interpreted collaboratively. |
| Collaborator Contribution | Technical advice and provision of high mass accuracy mass spectrometry |
| Impact | The main output of this collaboration has been method development |
| Start Year | 2020 |
| Description | Collaboration with Prof Kathryn Lilley: label-free quantification of Camelina seed proteins |
| Organisation | University of Cambridge |
| Department | Cambridge Centre for Proteomics |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Our team provided the biological question, grew plant material and extracted proteins. Data were discussed and interpreted collaboratively. |
| Collaborator Contribution | Technical advice and provision of high mass accuracy mass spectrometry |
| Impact | The main output of this collaboration has been method development |
| Start Year | 2020 |
| Description | Collaboration with Prof Rob Beynon |
| Organisation | University of Liverpool |
| Department | Centre for Proteome Research |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Co-design of methods |
| Collaborator Contribution | Co-design of methods (QConCATs for protein quantitation in transgenic Camelina sativa) |
| Impact | This collaboration started only recently, and is still in progress. |
| Start Year | 2020 |
| Description | Collaboration with Prof jean-Dennis Faure |
| Organisation | French National Institute of Agricultural Research |
| Country | France |
| Sector | Academic/University |
| PI Contribution | Selection of candidate genes and screening of generations |
| Collaborator Contribution | production of muliplex CRISPR lines in TAG assembly - Wt and elite camelina germplasm |
| Impact | Ongoing work |
| Start Year | 2019 |
| Description | Collaboraton with Yield10 Biosceinces |
| Organisation | Yield10 Bioscience |
| Country | United States |
| Sector | Private |
| PI Contribution | We have entered into a collaboration with the US-based agricultural biotechnology company Yield10 Biosciences, to explore the genetic improvement of Camelina, including the option to commercialize our omega-3 technology |
| Collaborator Contribution | CBI |
| Impact | Not yet realsed |
| Start Year | 2020 |
| Description | Composition and properties of B napus oleosome ghosts for use as a structuring and functional food ingredient |
| Organisation | Cargill Europe |
| Country | Belgium |
| Sector | Private |
| PI Contribution | Students and PDRAs training in lipid biochemistry analytical methods. Lipidomic analyses |
| Collaborator Contribution | Extraction, purification of OSR oil bodies, biophysical properties testing. |
| Impact | joint RRes-UoN iCASE PhD studentship |
| Start Year | 2017 |
| Description | Composition and properties of B napus oleosome ghosts for use as a structuring and functional food ingredient |
| Organisation | University of Nottingham |
| Department | University of Nottingham Museum |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Students and PDRAs training in lipid biochemistry analytical methods. Lipidomic analyses |
| Collaborator Contribution | Extraction, purification of OSR oil bodies, biophysical properties testing. |
| Impact | joint RRes-UoN iCASE PhD studentship |
| Start Year | 2017 |
| Description | Development of non-glaucous Brassica napus lines by genome editing |
| Organisation | Swedish University of Agricultural Sciences |
| Country | Sweden |
| Sector | Academic/University |
| PI Contribution | Biochemical and physiological characterisation of genome edited B.napus lines |
| Collaborator Contribution | generation of genome edited B. napus lines |
| Impact | work in progress |
| Start Year | 2018 |
| Description | Development of tandem fluorescent protein timer methodology |
| Organisation | Heidelberg University |
| Department | Centre for Organismal Studies |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | Co-development of research methodology |
| Collaborator Contribution | Co-development of research methodology |
| Impact | A joint publication is reported in the appropriate section |
| Start Year | 2017 |
| Description | Dr James Doughty, studentship |
| Organisation | University of Bath |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Increasing seed yields by the targeted modulation of brassinosteroids |
| Collaborator Contribution | To be entered |
| Impact | Ongoing |
| Start Year | 2018 |
| Description | Engineering novel seed storage lipids - ketocarotenoids and esters |
| Organisation | University of Nebraska-Lincoln |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Synthesis of genes for assembly and expression in transgenic plants - reciprocal exchange of transgenic material for environmental release (GM field trials) |
| Collaborator Contribution | Synthesis of genes for assembly and expression in transgenic plants - reciprocal exchange of transgenic material for environmental release (GM field trials) |
| Impact | None in the public domain. |
| Start Year | 2017 |
| Description | Evaluation of a plant hydrolase as an industrial enzyme |
| Organisation | Protein Technologies Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | We produced and supplied a recombinant plant hydrolase with a novel activity for application testing by Dr Dietmar Lang at Unilever R&D. |
| Collaborator Contribution | Unilever R&D carried out application testing in-house. |
| Impact | Work was supported by BBSRC HVCfP NIBB PoC grant. Material still being tested. |
| Start Year | 2018 |
| Description | Evaluation of a plant hydrolase as an industrial enzyme |
| Organisation | Unilever |
| Department | Unilever UK R&D Centre Port Sunlight |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | We produced and supplied a recombinant plant hydrolase with a novel activity for application testing by Dr Dietmar Lang at Unilever R&D. |
| Collaborator Contribution | Unilever R&D carried out application testing in-house. |
| Impact | Work was supported by BBSRC HVCfP NIBB PoC grant. Material still being tested. |
| Start Year | 2018 |
| Description | Field evaluation of gene-edited crops |
| Organisation | French National Institute of Agricultural Research |
| Department | INRA Versailles |
| Country | France |
| Sector | Academic/University |
| PI Contribution | Obtaining regulatory approval to carry out environmental release (field trial) of gene-edited camelina - this was the first release of it's kind in Europe. |
| Collaborator Contribution | Provision of characterised, gene-edited material for release |
| Impact | A paper was published eLife. |
| Start Year | 2018 |
| Description | Functional and biochemical characterisation of VLC-PUFA biosynthetic gene/enzyme candidates in yeast and Algae |
| Organisation | University of Huelva |
| Country | Spain |
| Sector | Academic/University |
| PI Contribution | cloning and functional characterisation of Algal Elongase genes in yeast Lipid analyses, GC-FID, GC-MS, ESI-MS/MS |
| Collaborator Contribution | Student/PDRA visiting workers Cultivation and analysis of algal strains |
| Impact | Research article in preparation |
| Start Year | 2018 |
| Description | Genetic improvement of Brassica oilseed crops |
| Organisation | University of York |
| Department | Biological Sciences |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Extraction, purification and analysis of lipids (biomass surface lipids, seed oil and oil minor components (wax esters and lipid soluble bioactives) Training of students and PDRAs in lipid biochemistry analytical techniques |
| Collaborator Contribution | Establishment of Brassica crop genomic resources Generation of a gamma-ray mutagenized B. napus population Screening and selection of mutagenized lines |
| Impact | RIPR and BBSRC-DBT Newton-PORI research fish entries |
| Start Year | 2017 |
| Description | Identification and characterization of a plant lipid biosynthesis pathway that supplies arbuscular mycorrhizal fungi with lipids |
| Organisation | University of Cambridge |
| Department | The Sainsbury Laboratory |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We characterised the enzyme activity of the RAM2 protein from Medicago truncatula, hypothesised that it played a role supplying lipids to arbuscular mycorrhizal fungi and devised a series of experiments involving plant expression for marker fatty acids and radiolabeling of mutants to prove this hypothesis. |
| Collaborator Contribution | Our partner (Prof Giles Oldroyd) first identified the RAM2 gene through a genetic screen, along with RAM1, characterised the mutants and demonstrated that RAM1 induced RAM2, as well as a other genes putatively involved in fatty acid and lipid biosynthesis and transport. Our partner also generated constructs and transgenic lines to allow us to carry out experiments to show that arbuscular mycorrhizal fungi receive lipids from their host plant. |
| Impact | We published our work in 2017 (doi: 10.1126/science.aan0081). |
| Start Year | 2015 |
| Description | Investigating the dual protein-lipid antigenic response required for sensitisation to Brazil Nut Ber e 1 |
| Organisation | Universidade de São Paulo |
| Country | Brazil |
| Sector | Academic/University |
| PI Contribution | Synthesis and cloning of WT and mutant form of the Ber-e-1 gene. Camelina transformation Brazil nut lipid extraction and purification |
| Collaborator Contribution | In vitro assays with T-cells (UoN). Purified protein with and without lipid fractions Sensitization assays in vivo (in rats) |
| Impact | research manuscript in preparation |
| Start Year | 2018 |
| Description | Investigating the dual protein-lipid antigenic response required for sensitisation to Brazil Nut Ber e 1 |
| Organisation | University of Nottingham |
| Department | School of Biosciences |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Synthesis and cloning of WT and mutant form of the Ber-e-1 gene. Camelina transformation Brazil nut lipid extraction and purification |
| Collaborator Contribution | In vitro assays with T-cells (UoN). Purified protein with and without lipid fractions Sensitization assays in vivo (in rats) |
| Impact | research manuscript in preparation |
| Start Year | 2018 |
| Description | Investigating the role of rice receptor kinases in lipid supply to arbuscular mycorrhizal fungi. |
| Organisation | University of Cambridge |
| Department | Department of Plant Sciences |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We are analysing lipids and metabolism in the mutants to determine if they are defective in lipid supply to the fungus. |
| Collaborator Contribution | Our partner (Dr Uta Paszkowski) has identified receptor kinase mutants in rice that are weakly impaired in arbuscular mycorrhization but have a dramatic reduction in the formation of vesicles that store lipids. |
| Impact | Not yet. Work in progress. |
| Start Year | 2018 |
| Description | MIC of transgenic microalgae expressing antimicrobial peptides |
| Organisation | National Physical Laboratory |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Production of transgenic starins of microalgae expressing antimicrobial peptides |
| Collaborator Contribution | Measurement of inhibition activities of the transgenic strains |
| Impact | Ongoing work |
| Start Year | 2019 |
| Description | Metabolic and biochemical characterisation of cytosolic lipid droplets that appear to be associated with chronic kidney disease in domestic cat |
| Organisation | University of Nottingham |
| Department | School of Veterinary Medicine and Science Nottingham |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Lipidomic profiling of lipid droplets from wild and domestic cat kidneys GC-FID, GC-MS, HPLC,ESI-MS/MS Training of PhD student in lipid biochemistry analytical methods. |
| Collaborator Contribution | Subcellular fractionation of animal tissue extraction and purification of lipid droplets intervention studies in domestic cat (analysis of correlation between nutrition and chronic diseases) |
| Impact | on-going work |
| Start Year | 2018 |
| Description | Microcin expression |
| Organisation | Ingenza Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Production of microcin in host strains |
| Collaborator Contribution | To be entered |
| Impact | Ongoing work |
| Start Year | 2019 |
| Description | Optimising the processing of novel oils and wax esters |
| Organisation | Pilot Pflanzenöltechnologie Magdeburg |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | Provision of novel (GM) material containing non-native wax esters |
| Collaborator Contribution | Extraction and evaluation of novel wax esters from GM plant seeds |
| Impact | . |
| Start Year | 2017 |
| Description | Plant wax esters as biolubricants |
| Organisation | Cargill |
| Country | United States |
| Sector | Private |
| PI Contribution | Provision of novel non-GM germplasm for evaluation and extraction |
| Collaborator Contribution | Deployment of proprietary know-how and capabilities to assess potential new product leads |
| Impact | None yet |
| Start Year | 2017 |
| Description | Production of human milk fat substitute in oilseed rape |
| Organisation | Cargill |
| Country | United States |
| Sector | Private |
| PI Contribution | We generated gene constricts and genetically transformed proprietary oilseed rape material provided by Kristin Gray at Cargill in order to produce seeds containing oil with the same sterioisomeric structure as human milk fat. |
| Collaborator Contribution | Cargill provided their proprietary oilseed rape material and genetic markers for their HOLL trait. |
| Impact | None yet. Material still to be fully characterised. |
| Start Year | 2018 |
| Description | Production of human milk fat substitute in oilseed rape |
| Organisation | National Institute of Agronomy and Botany (NIAB) |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We generated gene constricts and genetically transformed proprietary oilseed rape material provided by Kristin Gray at Cargill in order to produce seeds containing oil with the same sterioisomeric structure as human milk fat. |
| Collaborator Contribution | Cargill provided their proprietary oilseed rape material and genetic markers for their HOLL trait. |
| Impact | None yet. Material still to be fully characterised. |
| Start Year | 2018 |
| Description | Quantitative proteomics for analysis of targeted protein degradation |
| Organisation | University of Cambridge |
| Department | Cambridge Centre for Proteomics |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Provision of plant biology and protein labelling expertise |
| Collaborator Contribution | Provision of mass spectrometry expertise |
| Impact | Joint publications are listed the relevant section. |
| Start Year | 2013 |
| Description | RES ISF call |
| Organisation | French Agricultural Research Centre for International Development |
| Country | France |
| Sector | Private |
| PI Contribution | Generation and analyses of high resolution image data for around 650 rice lines. |
| Collaborator Contribution | Developing new image analyses tools specifically for rice roots |
| Impact | New analysis pipelines are being tested. The collaboration uses a combination of expertise in seedling vigour, phenotyping, and image analyses. |
| Start Year | 2021 |
| Description | Rothamsted Research- CSIRO Linkage proposals, Collaboration with Dr Matthew Nelson |
| Organisation | Commonwealth Scientific and Industrial Research Organisation |
| Country | Australia |
| Sector | Public |
| PI Contribution | To be entered |
| Collaborator Contribution | To be entered |
| Impact | Ongoing work |
| Start Year | 2019 |
| Description | SWBio-DTP studentship |
| Organisation | University of Bath |
| Department | Department of Biology and Biochemistry |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | A new PhD project on 'Increasing seed yields by the targeted modulation of brassinosteroids (BR)' has been initiated with the University of Bath (Dr James Doughty). Recent preliminary research in the Kurup lab identified novel BR related loci that play a role in increased ovule primordia. We have generated transgenic lines in Arabidopsis that have increased ovule number in their gynoecia compared to control plants. This collaboration is to further investigate these findings. |
| Collaborator Contribution | . |
| Impact | This project will include a variety of disciplines- molecular biology, genetics, developmental cell biology, imaging, and bioinformatics. The collaboration outlined in this proposal is designed to benefit from the complimentary expertise and experimental resources within each institution. |
| Start Year | 2022 |
| Description | Seed Vigour in OSR |
| Organisation | Elsoms Seeds |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Optimise the high throughput seed vigour phenotyping platform previously develop for monocot crop species for OSR. |
| Collaborator Contribution | Access to elite varieties and input re desirable traits |
| Impact | analyses of seed vigour in Brassica diversity set. |
| Start Year | 2021 |
| Description | Synthetic Biology - Foundry validation |
| Organisation | University of Liverpool |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Design, synthesis and conceptualisation of modular multi-component plant transformation vectors for advanced metabolic engineering |
| Collaborator Contribution | Optimise and validate the combinatorial assembly of multi-gene constructs for plant transformation. |
| Impact | None in the public domain yet. |
| Start Year | 2018 |
| Description | new collaboration |
| Organisation | University of Bath |
| Department | Department of Biology and Biochemistry |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | A new PhD project on 'Increasing seed yields by the targeted modulation of brassinosteroids (BR)' has been initiated with the University of Bath (Dr James Doughty). Recent preliminary research in the Kurup lab identified novel BR related loci that play a role in increased ovule primordia. We have generated transgenic lines in Arabidopsis that have increased ovule number in their gynoecia compared to control plants. This collaboration is to further investigate these findings. |
| Collaborator Contribution | The Bath partners will help with the characterisation of the above phenotypes, in particular investigations on the development of post zygotic barriers, if any in these lines. They will also help with semi-restricted seed yield experiments. |
| Impact | This project will include a variety of disciplines- molecular biology, genetics, developmental cell biology, imaging, and bioinformatics. The collaboration outlined in this proposal is designed to benefit from the complimentary expertise and experimental resources within each institution. |
| Start Year | 2017 |
| Title | DESATURASE AND METHOD FOR THE PRODUCTION OF POLYUNSATURATED FATTY ACIDS IN TRANSGENIC ORGANISMS |
| Description | The present invention relates to a polynucleotide from Emiliana huxleyi which codes for a desaturase and which can be employed for the recombinant production of polyun- saturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotide according to the invention, and to the polypeptides encoded by the polynucleotide. The invention furthermore relates to antibodies against the polypeptide according to the invention. Finally, the invention also relates to production methods for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements. |
| IP Reference | CA2723072 |
| Protection | Patent granted |
| Year Protection Granted | 2009 |
| Licensed | Commercial In Confidence |
| Impact | . |
| Title | Genetically modified plants with enhanced drought tolerance |
| Description | This invention relates to genetically modified plants with improved drought tolerance and to genetically modified cells thereof. |
| IP Reference | |
| Protection | Patent / Patent application |
| Year Protection Granted | 2023 |
| Licensed | No |
| Title | Genetically modified plants with enhanced drought tolerance |
| Description | This invention relates to plants with improved drought tolerance, leading to improved yields of crop plants under variable field conditions. The plants are genetically modified plants |
| IP Reference | P239758GB/RAW |
| Protection | Patent application published |
| Year Protection Granted | 2019 |
| Licensed | Commercial In Confidence |
| Impact | The modified plant materials and germplasm have potential application in the generation and breeding of new more drought resistant plants, including modification of existing plant varieties to improve field performance. |
| Title | Improved methods from the production of EPA and DHA |
| Description | Improved traits in transgenic camelina |
| IP Reference | |
| Protection | Patent application published |
| Year Protection Granted | 2021 |
| Licensed | Commercial In Confidence |
| Impact | This technology is under evaluation by a third party |
| Title | NON-HUMAN ORGANISM FOR PRODUCING TRIACYLGLYCEROL |
| Description | Described herein are non-human organisms for producing triacylglycerol wherein the non- human organism is genetically modified to express a lysophosphatidic acid acyltransferase specific for C16:0-Coenzyme A (C16:0 LPAT) and wherein, once expressed, the C16:0 LPAT is localised in the endoplasmic reticulum. Also described are non-human organisms for producing triacylglycerol in which (a) a lysophosphatidic acid acyltransferase specific for C16:0-Coenzyme A (C16:0 LPAT) is localised in the endoplasmic reticulum; and (b) activity of native endoplasmic reticulum lysophosphatidic acid acyltransferase (ER LPAT) is suppressed or prevented. Further described are non-human organisms in which a chloroplast lysophosphatidic acid acyltransferase (LPAT) is expressed, wherein said chloroplast LPAT lacks a functional chloroplast targeting signal. The disclosure also relates to methods for extracting triacylglycerol from the organisms and the use thereof in infant formula. |
| IP Reference | WO2021023991 |
| Protection | Patent application published |
| Year Protection Granted | 2021 |
| Licensed | No |
| Impact | Industrial partner supporting a BBSRC follow on fund application to develop technology. |
| Title | Novel fatty acid desaturases and elongases and uses thereof |
| Description | The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturases and elongases from the organism Emiliana huxleyi. The invention also provides recombinant expression vectors containing desaturase or elongase nucleic 5 acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g. arachidonic acid (ARA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA). 3068284_1 (GHMatters) P89302.AU |
| IP Reference | AU2018282290 |
| Protection | Patent application published |
| Year Protection Granted | 2019 |
| Licensed | Commercial In Confidence |
| Impact | This patent protects the discover of an acyl-CoA dependent D5-desaturase, a key step in the production of EPA in transgenic plants and algae. The patent covers the use of this gene in both transgenic systems. |
| Title | Process for producing a polyunsatured fatty acid comprising arachidonic acid |
| Description | The invention relates to nucleic acid derived from Perkinsus marinus which encodes a 9-elongase, a 8-desaturase and a 5-desaturase enzyme. All of the coding sequences can be transcribed as a single transcript. |
| IP Reference | EP2380984 |
| Protection | Patent granted |
| Year Protection Granted | 2011 |
| Licensed | Commercial In Confidence |
| Impact | Part of the IP portfolio around which commercialisation is focused |
| Title | Production of Omega-3 Long Chain Polyunsaturated Fatty Acids |
| Description | A recombinant camelina plant or cell comprising one or more polynucleotides encoding a A6-desaturase, a A6-elongase and a A5-desaturase operably linked with one or more regulatory sequences. |
| IP Reference | |
| Protection | Patent application published |
| Year Protection Granted | 2021 |
| Licensed | Commercial In Confidence |
| Impact | Under evaluation as part of an option agreement with a third party. |
| Title | Production of omega-3 long chain polyunsaturated fatty acids |
| Description | A recombinant camelina plant or cell comprising one or more polynucleotides encoding a &Dgr;6-desaturase, a &Dgr;6-elongase and a &Dgr;5-desaturase operably linked with one or more regulatory sequences. |
| IP Reference | AU2013246661 |
| Protection | Patent granted |
| Year Protection Granted | 2014 |
| Licensed | Commercial In Confidence |
| Impact | Foundation of an IP portfolio with which to commercialise the omega-3 technology |
| Title | Production of omega-3 long chain polyunsaturated fatty acids |
| Description | Generation of EPA and DHA in transgenic camelina, suitable for use as a feed or food ingredient. Patent provides broad protection of our key technology till 2033 |
| IP Reference | AU 2019201875 B2 |
| Protection | Patent granted |
| Year Protection Granted | 2021 |
| Licensed | Commercial In Confidence |
| Impact | This patent is part of of a portfolio of IP under commercial evaluation by a third party |
| Title | Recombinant camelina plant or cell |
| Description | Broad protection of our camelina platform for the production of EPA+DHA in transgenic plants. A divisional has been filled to extend protection further (see separate entry). The USA is the primary location for the commercialization of this technology so having a strong IP position based on granted patents is highly desirable. |
| IP Reference | |
| Protection | Patent granted |
| Year Protection Granted | 2021 |
| Licensed | Commercial In Confidence |
| Impact | Evaluation of this patent is being carried out as part of an options-agreement with a third party. |
| Title | Recombinant camelina plant or cell |
| Description | Granted broad claims in Canada protecting our GM camelina platform for the production of EPA and DHA |
| IP Reference | 2869738 Canada |
| Protection | Patent granted |
| Year Protection Granted | 2021 |
| Licensed | Commercial In Confidence |
| Impact | This patent is part of a portfolio of IP being commercially evaluated by a third party |
| Title | Recombinant camelina plant or cell (US Divisional) |
| Description | Divisional application of recent grant 10881631B2, extending the scope of our applications and claims. |
| IP Reference | |
| Protection | Patent application published |
| Year Protection Granted | 2022 |
| Licensed | Commercial In Confidence |
| Impact | This application is part of a portfolio of IP being evaluated by a third party |
| Title | TRANSGENIC MICROALGAE WITH INCREASED PRODUCTION OF AT LEAST ONE OMEGA-3 LONG CHAIN POLYUNSATURATED FATTY ACID |
| Description | The invention relates to genetically modified organisms with enhanced production of omega-3 long chain polyunsaturated fatty acids. |
| IP Reference | US2018312888 |
| Protection | Patent application published |
| Year Protection Granted | 2018 |
| Licensed | Commercial In Confidence |
| Impact | Establishing and validating novel platforms for the production of omega-3 LC-PUFAs |
| Description | 22nd International Symposium on Plant Lipids, Goettingen |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Poster presentation Development of an ESI-MS/MS method for high throughput profiling of free and esterified sterols and Associative Transcriptomic analysis of seed phytosterol content and composition using a B. napus diversity panel Richard Broughton1, Lenka Havlickova2, Andrea Harper2, Ian Bancroft2 and Frédéric Beaudoin1 1Biological Chemistry and Crop Protection Department, Rothamsted Research, Harpenden, AL5 2JQ, UK. 2 Department of Biology, University of York, Heslington, YO10 5DD, UK. Oilseed rape (Brassica napus) is a commercially viable crop owing partly to the seed oil co-products which can be exploited commercially, adding value to the crop. One emerging "bio-refining" opportunity in rapeseed involves the extraction and purification of phytosterols from the oil. Phytosterols are industrially important due to their increasing use as both a functional food component and as nutraceuticals (role in lowering LDL cholesterol). Phytosterols exist in two predominant forms in Brassica napus seeds, free sterols and steryl esters, and it is the latter that is of greater industrial use because of their increased solubility in fat and oil based food products. Although the biosynthetic pathways involved in the synthesis of plant sterols are known to some extent from studies conducted in species such as Arabidopsis thaliana, the quantitative control of their accumulation (key to yield of these compounds) is not understood. In addition, as seed phytosterol content has not been the foci for crop improvement by breeders, there is no improved germplasm or molecular markers available to aid breeding. We have developed a high throughput ESI-MS/MS method for the compositional and content analysis of seed sterols and steryl esters in a B. napus diversity collection (over 400 accessions representing the ASSYST panel). Here we report the quantitative variations observed and the identification of sequence (SNPs) and gene expression markers (GEMs) associated with these traits in both the A and the C genomes. The strong correlation observed between SNPs and GEMs will greatly facilitate the identification and cloning of the underlying causal genes. Interestingly, we found that very long chain fatty acids are completely excluded from the steryl ester fraction, regardless of the seed fatty acid composition, and that steryl ester content strongly correlates with the seed total sterol content. |
| Year(s) Of Engagement Activity | 2016 |
| URL | http://www.eurofedlipid.org/meetings/archive/goettingen2016/ |
| Description | 8th European Symposium on Plant Lipids |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Scientific presentation, Monday 3rd July 2017: High-throughput Profiling of Free and Esterified Sterols and Associative Transcriptomics Analysis of Seed Phytosterol Content and Composition in a B. napus diversity Panel (ASSYST) R. Broughton, Harpenden/GB , L. Havlickova, York/GB, A. Harper, York/GB, I. Bancroft, York/GB, F. Beaudoin, Harpenden/GB |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://www.eurofedlipid.org/meetings/malmoe2017/index.php#programme |
| Description | 8th European Symposium on Plant Lipids, Malmö, Sweden |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I presented research findings regarding our discovery that plants supply arbuscular mycorrhizal fungi with lipids as a major carbon source. |
| Year(s) Of Engagement Activity | 2017 |
| Description | AHDB Farmer visit |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Visit by interested farmers associated with AHDB, with a view to learning more about innovative approaches to food security |
| Year(s) Of Engagement Activity | 2021 |
| Description | BBC Countryfile coverage of GE field trials at RRes |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | An interview with BBC Countryfile presenter Tom Heap, carried out at the RRes GM and GE field trial site at Harpenden. Discussing the potential of GE and the future regulation of this technology post-EU |
| Year(s) Of Engagement Activity | 2020 |
| Description | BBC Radio 4 |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Presentation of our work on BBC Radio 4 in January 2021 (BBC Radio 4 - 39 Ways to Save the Planet, Super Rice). This was a great opportunity for the team to discuss the ongoing research and elaborate on a successful international collaboration which will initially benefit rice farmers in low and middle income countries but in the longer run should also help mitigate greenhouse gas emissions produced by the puddled transplanting method of growing rice. |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://www.bbc.co.uk/programmes/m000qwt1 |
| Description | BBSRC UK Brassica strategy meetings |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Other audiences |
| Results and Impact | Represented Rothamsted a strategic meetings organised by BBSRC to discuss future research and funding relevant to the UK Brassica research community. Presentation of RRes past and current research involving Brassica crops in front of academic and industrial stakeholders and representatives of funding bodies (BBSRC, DEFRA, ADAS, AHBD). |
| Year(s) Of Engagement Activity | 2017,2018 |
| Description | BBSRC workshop on Engineering Biology |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | An online workshop to define and refine the UK focus in the area of Engineering Biology (aka applied synthetic biology) |
| Year(s) Of Engagement Activity | 2020 |
| Description | British Science Week 2021 poster trail involvement |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Participated in Rothamsted Research's British Science Week poster trail by creating a 'Meet the scientists' poster with a QR code link to a short video I made about GM crops. Posters will be displayed for 5th-14th March on a poster trail around Harpenden for school children to view. Scanning the QR code on the poster will take the audience to the GM crop video on YouTube. |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://twitter.com/LangdonMollie/status/1369315061626511374 |
| Description | Cafe Scientifique public outreach scientific talks 2019-2020 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | Cafe Scientifique is a monthly event run by PhD students which hosts scientific talks for the general public held in a pub. I was involved in organising and hosting the events (liaising with venue holder, advertising events, chairing talks and Q&A sessions). 10-30 members of the public were regularly attending the monthly talks and were engaging in interesting discussion during the Q&A sessions. Current events are postponed due to COVID-19 restrictions. |
| Year(s) Of Engagement Activity | 2019,2020 |
| URL | https://twitter.com/csharpenden?lang=en |
| Description | DEFRA short video on GE |
| Form Of Engagement Activity | Engagement focused website, blog or social media channel |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | I was filmed by the Defra Comms unit, explaining the opportunities associated with gene editing |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://twitter.com/i/status/1443127318390575108 |
| Description | Engagement with industry |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | We are using the phenotyping platform generated as part of the project to test on crop species of interest to Elsoms. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Engagement with industry/stakeholder |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Presented by Olga Sayanova outputs from metabolic engineering of microalgae |
| Year(s) Of Engagement Activity | 2017 |
| Description | Engagement with industry/stakeholder |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Visit and presentation of phenotyping technolgies (by S Kurup) to Elsoms |
| Year(s) Of Engagement Activity | 2019 |
| Description | Engagement with industry/stakeholder |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Meeting with Elsoms on several aspects of seed/reproductive research as avenues for future collaborations. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Engagement with industry/stakeholder |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | 2nd Annual BRAVO sLoLa stakeholder meetings where members of the breeding/growing industry discuss progress on the BRAVO project. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Engagement with industry/stakeholder |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Visit and presentation of phenotyping technolgies (by S Kurup) to Plant Impact |
| Year(s) Of Engagement Activity | 2019 |
| Description | Engagement with industry/stakeholder |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | The second annual 'Brassica Rapeseed And Vegetable Optimisation' stakeholder meeting was organised on the 19the of February 2019 at Rothamsted Research. Besides project partners and members of the supervisory board, over 20 stakeholder/companies were invited to the meeting. Along with project presentation, vegetable and oilseed breeders presented challenges faced by them and this was followed by several feedback and discussion sessions. |
| Year(s) Of Engagement Activity | 2019 |
| Description | First Annual Stakeholder Meeting |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | The first Annual BRAVO stakeholder meeting was organised on the 1st of February 2018 at Rothamsted Research. Besides project partners and members of the supervisory board, over 21 stakeholder/companies were invited to the meeting. Along with project presentation, vegetable and oilseed breeders presented challenges faced by them and this was followed by several feedback and discussion sessions. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Fish Farmer Article |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Fish Farmer Magain - Volume 41 Number 01 January 2018. Article 'Man on a Mission' pg 30-33 |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://issuu.com/fishfarmermagazine/docs/fish_farmer_january_2018 |
| Description | Freddie Theodoulou presentation "Plant Proteostasis; controlling protein stability for sustainable agriculture" |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Freddie Theodoulou presented "Plant Proteostasis; controlling protein stability for sustainable agriculture" at New Phytologist Workshop, University of Durham, UK |
| Year(s) Of Engagement Activity | 2018 |
| Description | Front-cover feature in BBSRC Business magazine |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | This was a co-ordinated media campaign, working with BBSRC and the BBC to run the story about our GM camelina oil being used in a salmon feeding study in a sea loch. The story was run on multiple platforms, BBC TV, BBC radio and website |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.bbc.co.uk/news/science-environment-44743003 |
| Description | Global Omega-3 Forum |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Attendance at GoEd Exchange 2018 - contribute to panel discussion on GM Omega-3 |
| Year(s) Of Engagement Activity | 2018 |
| Description | Grand Challenges Meeting, Washington, DC |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I was invited to attend the BMG-funded Grand Challenges Meeting, where I participated in the crops session workshop and presented flash talks on our GCRF BBSRC-funded work on improving seed vigour for direct-seeded rice and our work on nutrient exchange in the plant arbuscular mycorrhizal association. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Hidden Hunger - an online expert discussion organised by Merck and Nature Food |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I was part of a small panel of experts discussing the contribution the GM & GE could make to nutritional enhancement of feed and food. |
| Year(s) Of Engagement Activity | 2021 |
| Description | Hosted pupils from 2 secondary schools from Harpenden in Bioimaging |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Organised by S Kurup, Bioimaging staff introducing imaging technologies (light, confocal, EM) and insights into our research |
| Year(s) Of Engagement Activity | 2017 |
| Description | Hosted pupils from 2 secondary schools from Harpenden in Bioimaging |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Organised by S Kurup, Bioimaging staff introducing imaging technologies (light, confocal, EM) and insights into our research |
| Year(s) Of Engagement Activity | 2018 |
| Description | Hosted two secondary school pupils from St Albans Girls' School for work shadowing |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Freddie Theodoulou and Chelsea Rundle hosted two secondary school pupils from St Albans Girls' School for work shadowing |
| Year(s) Of Engagement Activity | 2019 |
| Description | Hosted visit by local school children |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | I hosted a visit by local 6th form school children to investigate Rothamsted Research and the work we do. This involved describing the TPM and other ISPG programs, observing and learning about wheat transformation, EM and light microscopy, field phenotyping facilities, the insect survey and the GM field site and long term experiments. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Hosted visit by local school children |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | I hosted a visit by local 6th form school children to investigate Rothamsted Research and the work we do. This involved describing the TPM and other ISPG programs, observing and learning about wheat transformation, EM and light microscopy, field phenotyping facilities, the insect survey and the GM field site and long term experiments. |
| Year(s) Of Engagement Activity | 2018 |
| Description | International Plant Systems Biology Meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Poster presentation at the virtual International Plant Systems Biology meeting on 'Gene regulatory networks for polyploid and diploid Brassica sp. during reproductive transitions'. |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://meetings.embo.org/event/20-plant-systems |
| Description | Interview with SCIENCE magazine regarding gene editing and ECJ ruling |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | I was interviewed by Science magazine for my response to the ECJ ruling on gene editing, based on the fact that I was running the only overt GE field trial in Europe |
| Year(s) Of Engagement Activity | 2018 |
| Description | Interview with WIRED magazine on gene editing |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | I was interviewed to provide my view on the recent ECJ court case regarding legal status of gene editing in Europe. Interview was part of a larger item on the potential of gene editing. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.wired.co.uk/article/cavendish-banana-extinction-gene-editing |
| Description | Invited Departmental presentation University of Buenos Aires, Argentina |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation on Engineering plants for nutrition |
| Year(s) Of Engagement Activity | 2017,2019 |
| Description | Invited Departmental presentation University of Buenos Aires, Argentina |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Plant membrane remodelling in response to abiotic stress |
| Year(s) Of Engagement Activity | 2019 |
| Description | Invited Lecture, Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Lecture title: Oilseed crops as integrated production platforms for food, feed, fuel and renewable industrial feedstock |
| Year(s) Of Engagement Activity | 2016 |
| Description | Invited Talk ISFNF aquaculture congress |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Johnathan Napier gave an invited talk at the ISFNF aquaculture congress in Gran Canaria |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited lecture at Plant Proteostasis 2019, Freiburg, Germany |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Freddie Theodoulou gave a presentation on tandem timer technology |
| Year(s) Of Engagement Activity | 2019 |
| Description | Invited lecture at University of York |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Freddie Theodoulou gave a presentation of roles of proteostasis in plants |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited lecture at Utrecht University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Freddie Theodoulou gave a presentation of roles of proteostasis in plants |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited lecture, Institute of Life Sciences Jiangsu University, Zhenjiang, China |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | Lecture title: Oilseed crops as integrated production platforms for food, feed, fuel and renewable industrial feedstock. 1 h lecture Including a presentation of ther power of Associative transcriptomics for improving the value of co-products in oilseed rape (B. napus). |
| Year(s) Of Engagement Activity | 2016 |
| Description | Invited lecture, Intituto de la Grasa, Seville |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Plant biotechnology and biorefinning lecture: Oleaginous crops as integrated production platforms for sustainable production of food, feed, fuel and renewable industrial feedstock Initiated discussions about future collaboration on transaltional research projects and students exchange/training. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited lecture, Workshop on Integrative Plant Biology Wuhan, China |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Huazhong (HZAU) - University of Nebraska Lincoln (UNL) Joint workshop on Integrative Plant Biology: Innovations in Crop Improvement to Meet the Global Grand Challenges of the 21st Century. September 2016 Lecture title: Associative transcriptomic approach to improving the value of co-products in oilseed rape (B. napus). |
| Year(s) Of Engagement Activity | 2016 |
| Description | Invited presentation at "Plant Proteostasis at the N-terminus", Halle, Germany |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Freddie Theodoulou gave a presentation of techniques for studying roles of the N-degron pathway in plant proteostasis |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited seminar, Department of Plant Biochemistry, Georg-August-Universität, Göttingen |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | I presented our recent findings that plants supply arbuscular mycorrhizal fungi with lipids as their major carbon source. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited seminar/lecture on Plant Biotechnology and biorefining - UNICAMP Sao Paulo. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Study participants or study members |
| Results and Impact | Lecture on Plant biotechnology and Biorefining illustrated by examples of successful research project carried out at Rothamsted research. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Keynote lecture - 17th EuroFed Lipid Congress in Seville |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited lecture: New insights in the composition of wax and sterol esters in seed oils revealed by ESI-MS/MS |
| Year(s) Of Engagement Activity | 2019 |
| URL | http://www.eurofedlipid.org/pages/sevilla |
| Description | Keynote speaker at Future Foods symposium |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Johnathan Napier was Keynote Speaker at Future Foods symposium, Koln (5-7 September 2018) |
| Year(s) Of Engagement Activity | 2018 |
| Description | Lecture plus careers Q & A. Plants for the Future. Plant Biotechnology, University of Cardiff. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Postgraduate students |
| Results and Impact | Lecture to post graduate students on the use of biotechnology approaches for crop improvement. Followed by Q & A on career options and pathways in plant sciences. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Lecture to undergraduate students |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Undergraduate students |
| Results and Impact | Lecture given to Plant Science module undergraduate students |
| Year(s) Of Engagement Activity | 2021 |
| Description | Lecture to undergraduate students |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Undergraduate students |
| Results and Impact | A lecture on this project and related research and its impact in general terms was presented to 2nd year undergraduate students specialising in Plant Sciences module from Oxford Brookes University. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Lecture to undergraduate students |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Undergraduate students |
| Results and Impact | Lecture to 2nd year undergraduate students |
| Year(s) Of Engagement Activity | 2022 |
| Description | Lecture to undergraduate students |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Undergraduate students |
| Results and Impact | A lecture on this project and related research and its impact in general terms was presented to 2nd year undergraduate students specialising in Plant Sciences module from Oxford Brookes University. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Mentoring Crest Students |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Mentored students completing their gold crest award in plant biology through the STEM ambassadors scheme. |
| Year(s) Of Engagement Activity | 2022 |
| Description | New Scientist Live virtual 'Future of Food and Agriculture' 2020 event participation |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Participated by creating a short video on GM crops that was broadcasted on Rothamsted Research's virtual channel during the New Scientist Live virtual 'Future of Food and Agriculture' event. The video was watched by a wide range of audience members (general public, scientific, industry etc.) and the video was later uploaded to Rothamsted Research's YouTube channel and received further views. |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://www.youtube.com/watch?v=d5Qm5q5sY1A |
| Description | Olga Sayanova invited speaker, Conference on Algal Biomass and Bioproducts |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Olga Sayanova was an invited speaker at Conference on Algal Biomass and Bioproducts, Seattle, USA |
| Year(s) Of Engagement Activity | 2018 |
| Description | Omega-3s from transgenic plants and its role in aquaculture |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | . |
| Year(s) Of Engagement Activity | 2019 |
| Description | Online event (Stevenage, Hertfordshire) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | LV Michaelson gave a short presentation on plant molecular biology and biochemistry to GCSE students, 11/2020. Approx.60 students attended from Thomas Alleyne School, Stevenage at an event targeted at girls. The teachers felt it had gone well and that students were more interested in science as a result. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Participated in virtual 2021 'Work Experience Meet a Scientist' session |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Participated in Rothamsted Research's 2021 virtual 'Work Experience Meet a Scientist' session (held online) - where I spoke to ten 15-16 year old school children who were on their 'virtual' work experience about what it is like to work in science and do a PhD. |
| Year(s) Of Engagement Activity | 2021 |
| Description | Peter Eastmond Presented work to industry (Inbiose) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Letter of support |
| Year(s) Of Engagement Activity | 2020 |
| Description | Peter Eastmond invited seminar speaker at University of Edinburgh |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Peter Eastmond invited seminar speaker at University of Edinburgh |
| Year(s) Of Engagement Activity | 2020 |
| Description | Peter Eastmond invited seminar speaker at Washington State University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Peter Eastmond invited seminar speaker at Washington State University |
| Year(s) Of Engagement Activity | 2020 |
| Description | Peter Eastmond presented at UKBRC2020 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Peter Eastmond presented at UKBRC2020 |
| Year(s) Of Engagement Activity | 2020 |
| Description | Peter Eastmond presented work to industry (AAK) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Material testing and partnership in funding application |
| Year(s) Of Engagement Activity | 2020 |
| Description | Peter Eastmond presented work to industry (BASF) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Industry funding application |
| Year(s) Of Engagement Activity | 2020 |
| Description | Peter Eastmond presented work to industry (Barenbrug) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Presentation of new finding and discussion over collaboration |
| Year(s) Of Engagement Activity | 2022 |
| Description | Peter Eastmond presented work to industry (Barenbrug) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Industry funding application |
| Year(s) Of Engagement Activity | 2020 |
| Description | Peter Eastmond presented work to industry (Cargill) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Material testing and letter of support |
| Year(s) Of Engagement Activity | 2020 |
| Description | Peter Eastmond presented work to industry (Germinal) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Disclose invention and discuss collaboration. |
| Year(s) Of Engagement Activity | 2023 |
| Description | Peter Eastmond presented work to industry (Nestle) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Material testing |
| Year(s) Of Engagement Activity | 2020 |
| Description | Peter Eastmond presented work to industry (Xylome) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Letter of support |
| Year(s) Of Engagement Activity | 2020 |
| Description | Peter Eastmond presented work to venture capital firm (GPV) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Peter Eastmond presented work to venture capital firm (GPV) |
| Year(s) Of Engagement Activity | 2020 |
| Description | Peter Eastmond presented work to venture capital firm (PoV) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Peter Eastmond presented work to venture capital firm (PoV) Letter of support |
| Year(s) Of Engagement Activity | 2020 |
| Description | Plant Biology 2021 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Oral presentation at Plant Biology 2021 Worldwide Summit organised by the American Society of Plant Biologists. |
| Year(s) Of Engagement Activity | 2021 |
| Description | Plant Lipids session at EuroFed Lipid Annual Congress, Belfast |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Johnathan Napier Organized and chaired Plant Lipids session at EuroFed Lipid Annual Congress, Belfast (16-19 September 2018) |
| Year(s) Of Engagement Activity | 2018 |
| Description | Plenary lecture at the GIFS "Emerging Technologies for Global Food Security" Conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Johnathan Napier gave a plenary lecture at the GIFS "Emerging Technologies for Global Food Security" Conference in Saskatoon |
| Year(s) Of Engagement Activity | 2018 |
| Description | Poster presentation at 2019 'European Plant Sciences Retreat' conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Presented a poster on my PhD project at the 2019 'European Plant Sciences Retreat' conference hosted in Nottingham (UK). |
| Year(s) Of Engagement Activity | 2019 |
| Description | Poster presentation at 2021 'Plant Science for Climate Emergency' virtual conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented a poster outlining my PhD project at the 2021 'Plant Science for Climate Emergency' virtual conference (held online). |
| Year(s) Of Engagement Activity | 2021 |
| Description | Poster presentation at 2021 'SWBio DTP Virtual Annual Student Conference' conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Postgraduate students |
| Results and Impact | Presented a poster outlining my PhD project at the 2021 'SWBio DTP Virtual Annual Student Conference' (held online). |
| Year(s) Of Engagement Activity | 2021 |
| Description | Presentation and collaborative discussions with DOE (USA) funded Bigger and Better Brassicaceae consortium |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation of RRes Camelina activities to members of the Bigger and better Brassicaceae DOE consortium. Further discussins on areas of collaboration. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Presentation at 2019 'SWBio DTP Annual Student Conference' |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Postgraduate students |
| Results and Impact | Presented a flash talk on my PhD project at the 2019 'SWBio DTP Annual Student Conference' hosted in Exeter (UK). |
| Year(s) Of Engagement Activity | 2019 |
| Description | Presentation at 2021 'SWBio DTP Annual Student Virtual Conference' |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Postgraduate students |
| Results and Impact | Presented a talk outlining my PhD project at the 2021 'SWBio DTP Annual Virtual Student Conference' (held online). |
| Year(s) Of Engagement Activity | 2021 |
| Description | Presentation at 2021 UK-Brassica Research Community |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented my PhD project at the Annual UK-Brassica Research Community (UK-BRC) conference at Rothamsted Research, Harpenden (UK). |
| Year(s) Of Engagement Activity | 2021 |
| Description | Presentation at 3rd International Conference on Plant Synthetic Biology, Bioengineering, and Biotechnology |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Peter Eastmond gave a presentation titled "Engineering the Stereoisomeric Structure of Plant Triacylglycerol to Mimic Human Milk Fat" |
| Year(s) Of Engagement Activity | 2019 |
| Description | Presentation at 9th European Symposium on Plant Lipids |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Peter Eastmond gave a presentation titled "Rewriting Triacylglycerol Biosynthesis to Tailor Fatty Acyl Composition" |
| Year(s) Of Engagement Activity | 2016,2019 |
| Description | Presentation at ABBA conference, Seattle |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented results of our work on production of phytase in microalgae |
| Year(s) Of Engagement Activity | 2018 |
| Description | Presentation at ELM |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The identification and tissue-specific localisation of Long-Chain Omega-3 Fatty Acid-Containing Triacylglycerols in Camelina sativa Embryos using MSI. 10th to 12th April, European Lipidomics Meeting, Toulouse, France. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Presentation at ELM |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | High resolution accurate mass (HR/AM) lipidomic approaches to compare the incorporation of EPA and DHA from transgenic Camelina or fish oil into the blood lipids of healthy humans. European Plant Lipid Meeting, 7th - 10th July, Marseille, France |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | Presentation at ISPL, Japan, 2018 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented outputs from research on EPA biosynthesis in diatoms |
| Year(s) Of Engagement Activity | 2018 |
| Description | Presentation at New Scientist Live event |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | A live Q+A session at New Scientist Live event in November 2020 to discuss science and how imaging can help advance science. |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://live.newscientist.com/exhibitors/rothamsted-research#/ |
| Description | Presentation at UK Rice Consortium meeting for Early Career Researchers |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Postgraduate students |
| Results and Impact | This short meeting was focussed on providing a forum for all postdocs and PhD students involved in rice research projects to come together and exchange ideas and experience on working with rice in the UK. The meeting was very much led by the researchers themselves, providing them the opportunity to both network (find out about the range of rice research being performed across the country) but with a focus on the nuts-and-bolts of rice research in the lab and field. |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://www.dur.ac.uk/dccit/events/?eventno=46060 |
| Description | Presentation at UK-BRC 2020 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | New findings presented at the Annual UK-BRC conference held virtually in Nov 2020. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Presentation at international Conference: Exploiting tomato Andean landraces to improve heat stress |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation at an international meeting on plant physiology covering the work of the collaboration. Exploiting phenotypic plasticity from tomato Andean landraces to improve heat stress tolerance in the face of global warming de Haro LA, De Luca MB, Bianchetti R, Bermudez L, Cernadas A, Lira BS, Michaelson LV, Haslam RP, Rossi M, Carrari F. 2018. Fisiologia Vegetal Cordoba Argentina. 11 - 15th Nov. |
| Year(s) Of Engagement Activity | 2018 |
| URL | http://www.rafv-clafv2018.org/en |
| Description | Presentation at international Conference: Phytochrome-dependent temperature perception modulates isoprenoid metabolism in tomato. |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation at an international meeting on plant genetics covering the work of the collaboration. Phytochrome-dependent temperature perception modulates isoprenoid metabolism in tomato. Bianchetti R, De Luca MB, de Haro LA, Rosado D Bermudez L, Michaelson LV, Haslam RP, Rossi M, Carrari F. 2019. Simposio Brasileiro de Gentetica Molecular de Plantas. 29th Apr 3rd May. Sau Paulo, Brazil. |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://www.sbg.org.br/pt-br/eventos/vii-simposio-brasileiro-de-genetica-molecular-de-plantas/bem-vi... |
| Description | Presentation at international Conference: Phytochromes and temperature dependent perception in Tomato |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation at an international meeting on plant physiology covering the work of the collaboration. Phytochrome-dependent temperature perception modulates isoprenoid metabolism in tomato. Bianchetti R, De Luca MB, de HaroLA, Rosado D, Bermudez L, Michaelson LV, Haslam RP, Rossi M, Carrari F. 2018. Fisiologia Vegetal Cordoba Argentina. 11 - 15th Nov. |
| Year(s) Of Engagement Activity | 2018 |
| URL | http://www.rafv-clafv2018.org/en |
| Description | Presentation at international Conference: Plant Biology Europe |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation at an international meeting on plant physiology covering the work of the collaboration. Epigenetics basis of light-dependent phytochrome perception in tomato fruits |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://europlantbiology2020.org |
| Description | Presentation at international Conference: Temperature and isopreniod metabolism in Tomato |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation at an international meeting on plant physiology covering the work of the collaboration. Temperature modulates lipid and isoprenoids metabolism in tomato fruits through phytochrome-dependent regulation. Bianchetti R, De Luca MB, de Haro LA, Rosado D Bermudez L, Michaelson LV, Haslam RP, Rossi M, Carrari F. 2018. Fisiologia Vegetal Cordoba Argentina. 11 - 15th Nov. |
| Year(s) Of Engagement Activity | 2018 |
| URL | http://www.rafv-clafv2018.org/en |
| Description | Presentation at international Conference: Tomato fruit and leaf lipidomics |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Poster presentation at an international meeting on Lipidomics covering the work of the collaboration. Developing a high-throughput HR/AM method for the analysis of the lipidome in the tissues from tomato. Michaelson LV, de Haro LA, De Luca MB, Bianchetti R, Bermúdez L, Cernadas A, Conte M, Lira BS, Peralta IE, Rossi M, Carrari F, Haslam RP. 2018. 7th European Lipidomics Meeting, 26-29 September Leipzig Germany. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://home.uni-leipzig.de/elm2018/ |
| Description | Presentation to potential new collaborators at CIRAD |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Discussions with Dr Christophe PERIN at CIRAD, Montepelier (attended by Pete, Smita and Gui). Presentation from both sides regarding research, possible collaborations, new tools for analysing transcriptome data |
| Year(s) Of Engagement Activity | 2021 |
| Description | Presentation to the parliamentary all-party group on agriculture |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Johnathan Napier presented to the parliamentary all-party group on agriculture re: regulation of gene editing post-ECJ (10 October 2018) |
| Year(s) Of Engagement Activity | 2018 |
| Description | Presented talk at NFU Barnet & St Albans Branch Meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Talk presented on oilseed engineering |
| Year(s) Of Engagement Activity | 2022 |
| Description | Provided evidence to the Parliamentary Office of Science and Technology on recent advances in gene editing in agriculture |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | This is a POSTnote from the The Parliamentary Office of Science and Technology, synthesizing the views of UK experts on the potential of gene editing to contribute to food security. I contributed to the document and reviewed/edited it. |
| Year(s) Of Engagement Activity | 2022 |
| URL | https://post.parliament.uk/research-briefings/post-pn-0663/ |
| Description | Rothamsted Research 'Festival of Ideas' Open Weekend |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | showcasing oilseeds and camelina at Open Weekend |
| Year(s) Of Engagement Activity | 2018 |
| Description | Rothamsted/UNL seminar series |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Richard Haslam co ordinates the RRes/UNL Translational Plant Science Webinar Seminar Series, establishing a monthly virtual seminar series to discuss translational plant science |
| Year(s) Of Engagement Activity | 2020 |
| Description | Royal Society Public Dialogue on Genetic Techologies |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Public Dialogue re: genetic technologies -. a news briefing about the findings of the public dialogue will take place at the Royal Society on Wednesday 7 March 2018 @ 10:45am. |
| Year(s) Of Engagement Activity | 2018 |
| Description | School Visit (Hitchin, Hertfordshire) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Presentation on plant molecular biology and biochemistry to GCSE and A level students form the Hitchin consortium held at Hitchin Girls School, 18/10/2018. Approx. 200 students attended and I spoke to approximately thirty. Many students talked about genetic engineering of plants and one or two mentioned gene editing. There was interest in careers in science including work experience and the Nuffield scheme. |
| Year(s) Of Engagement Activity | 2018 |
| Description | School Visit (Stevenage, Hertfordshire) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Presentation on plant molecular biology and biochemistry to GCSE students, 16/11/2018. Approx.60 students attended from Thomas Alleyne School, Stevenage at an event targeted at girls. Students talked about genetic engineering of plants and protecting the environment. There was interest in careers in science including work experience and the Nuffield scheme. The teachers felt it had gone well and that students were more interested in science as a result. |
| Year(s) Of Engagement Activity | 2018 |
| Description | School visit (St Albans, Herts) |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Organised a visit of 6th form students from a local secondary school to lean about the institute and our research. |
| Year(s) Of Engagement Activity | 2023 |
| Description | School visit (Stevenage, Herts) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | LV Michaelson Presentation on plant molecular biology and biochemistry to GCSE students, 15/11/2019. Approx.60 students attended from Thomas Alleyne School, Stevenage at an event targeted at girls. Students talked about genetic engineering of plants and protecting the environment. There was interest in careers in science including work experience and the Nuffield scheme. The teachers felt it had gone well and that students were more interested in science as a result. |
| Year(s) Of Engagement Activity | 2019 |
| Description | School visit in Hertfordshire |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Visited a sixth form centre to give a 30 pupils a talk about GM and careers in research. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Seminar to Food Standards Agency on gene editing in plants |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Part of a series of talks organized by the FSA to increase awareness regarding gene editing in plants |
| Year(s) Of Engagement Activity | 2020 |
| Description | Smita Kurup participated in New Scientist Live event with a focus on ' How Bioimaging can help research' |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | focus on ' How Bioimaging can help research' |
| Year(s) Of Engagement Activity | 2020 |
| Description | Spain-UK Plant Biotechnology forum |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | A 2 day workshop organised by SRUK/CERU and the Embassy of Spain to highlight the importance of plant biotechnology to face future challenges, specifically to enhance collaboration between Spanish and British companies. |
| Year(s) Of Engagement Activity | 2022 |
| URL | https://sruk.org.uk/ceru-events/spain-uk-plant-biotechnology-event/ |
| Description | Sustainable St Albans festival |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | To inform the public regarding the research undertaken within Rothamsted and specifically in BRAVO. |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://sustainablestalbans.org/get-started/sust-festival/ |
| Description | Talk at Annual Stakeholder meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Annual BRAVO sLoLa stakeholder meetings where members of the breeding/growing industry discuss progress on the BRAVO project. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Talk at Farm Manager conference |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Other audiences |
| Results and Impact | The Director, Dr Achim Dobermann presented the work being carried out in BRAVO, and WP4 to the attendees at the Velcourt farm Manager's Conference on 12th January 2018. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Talk at the 16th Eurofed Lipid Congress - Belfast |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Presented our recently published work: "New insights in the composition of wax and sterol esters in common and mutant sunflower oils revealed by ESI-MS/MS" Demonstration of the impact of this work for oil authentication and detection of fraudulent mixture to an audience of academic and industrial professionals. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://veranstaltungen.gdch.de/tms/frontend/index.cfm?l=7660&modus= |
| Description | To provide case studies for Defra, relevant to Precision Breeding Bill |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | To provide case studies to highlight the potential benefits of gene editing technologies. |
| Year(s) Of Engagement Activity | 2022 |
| Description | UK Brassica Research Community meeting |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Other audiences |
| Results and Impact | Frederic Beaudoin organised and chaired the UK-Brassica Research Community (UK-BRC) meeting at Rothamsted on 9th May 2018 |
| Year(s) Of Engagement Activity | 2017,2018 |
| Description | UK Brassica Research Community meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Other audiences |
| Results and Impact | Presented outputs from research on yield traits |
| Year(s) Of Engagement Activity | 2019 |
| Description | Undergraduate Lecturing at the University of Nottingham |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Undergraduate students |
| Results and Impact | Biotechnology BSc -Molecular Pharming module Frederic Beaudoin Lecturing plant biotechnology and molecular pharming at the University of Nottingham. Particularly, the metabolic engineering of plant lipid composition for food and non-food industrial applications using research projects carried out at RRes as successful examples. |
| Year(s) Of Engagement Activity | 2016,2017,2018,2019 |
| Description | Undergraduate Lecturing at the University of Sheffield |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Undergraduate students |
| Results and Impact | Freddie Theodoulou gave lectures on flood toelrance in plants and on plant metabolic engineering: from basic research to commercialisation |
| Year(s) Of Engagement Activity | 2019 |
| Description | Visit with high school students |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | 'Meet the Scientist' virtual chat hosted with high school students to disseminate information about our research and generally encourage them regarding science. |
| Year(s) Of Engagement Activity | 2021 |
| Description | Workshop Global Bioeconomy Summit |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Johnathan Napier attended the Global Bioeconomy Summit in Berlin and presented at workshop |
| Year(s) Of Engagement Activity | 2018 |
| Description | Workshop presentation at the 30th International Conference on Arabidopsis Research, Wuhan, China |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Hongtao Zhang gave a presentation on tandem timer technology |
| Year(s) Of Engagement Activity | 2019 |
| Description | World AgriTech Panel discussion on gene-editing |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | I chaired the panel discussion on the future applications on gene editing in agriculture at World Agri-Tech Innovation Summit in London, Oct 2018. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://worldagritechinnovation.com/ |
| Description | panel discussion on potential of gene editing in agriculture at World AgriTecb summit |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Johnathan Napier chaired panel discussion on potential of gene editing in agriculture at World AgriTec B summit, London (16 October 2018) |
| Year(s) Of Engagement Activity | 2018 |
| Description | presentation by Laura Siles at UK-BRC Nov 2020 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Oilseed seed traits trade-offs' by Laura Siles at UK-BRC Nov 2020 |
| Year(s) Of Engagement Activity | 2021 |
| Description | visit by Stuart Agnew (UKIP MP) |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Policymakers/politicians |
| Results and Impact | 16th Aug 2018 - Johnathan Napier hosted a visit from Stuart Agnew (UKIP MP) |
| Year(s) Of Engagement Activity | 2018 |