Cereal seed composition and end use quality
Lead Research Organisation:
Rothamsted Research
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
Cereals are the major arable crops in the UK and provide food, feed and raw materials for industry. Different end uses require different composition in terms of the amounts and compositions of the major grain components: starch, protein and cell wall polysaccharides. This project therefore uses a range of approaches (including metabolomics, transcriptomics and transgenesis) to dissect the pathways and mechanisms that determine cereal grain composition and to exploit this information to tailor the composition for traditional and novel end use requirements.
Specific targets are:-
1. Manipulating the amounts and composition of wheat endosperm cell walls for health (dietary fibre), feed and processing (distilling, biofuels).
2. Understanding the mechanisms by which proteins determine grain mechanical and functional properties.
3. Manipulating the patterns of gene expression and pathways of protein trafficking and deposition to develop novel wheats with improved processing properties.
Specific targets are:-
1. Manipulating the amounts and composition of wheat endosperm cell walls for health (dietary fibre), feed and processing (distilling, biofuels).
2. Understanding the mechanisms by which proteins determine grain mechanical and functional properties.
3. Manipulating the patterns of gene expression and pathways of protein trafficking and deposition to develop novel wheats with improved processing properties.
Planned Impact
unavailable
Organisations
People |
ORCID iD |
| Peter Shewry (Principal Investigator) |
Publications
Konarev A
(2008)
Serine proteinase inhibitors in seeds of Cycas siamensis and other gymnosperms
in Phytochemistry
Konarev AV
(2011)
Characterization of a glutenin-specific serine proteinase of Sunn bug Eurygaster integricepts Put.
in Journal of agricultural and food chemistry
Kuktaite R
(2011)
Structure and morphology of wheat gluten films: from polymeric protein aggregates toward superstructure arrangements.
in Biomacromolecules
Lambourne J
(2010)
Characterisation of an s-type low molecular weight glutenin subunit of wheat and its proline and glutamine-rich repetitive domain
in Journal of Cereal Science
Le Gall G
(2011)
Profiling of intact wheat arabinoxylans by spectroscopy
in New Food
Leon E
(2010)
Pasting properties of transgenic lines of a commercial bread wheat expressing combinations of HMW glutenin subunit genes
in Journal of Cereal Science
León E
(2010)
Stacking HMW-GS transgenes in bread wheat: Combining subunit 1Dy10 gives improved mixing properties and dough functionality
in Journal of Cereal Science
León E
(2009)
Mixing properties and dough functionality of transgenic lines of a commercial wheat cultivar expressing the 1Ax1, 1Dx5 and 1Dy10 HMW glutenin subunit genes
in Journal of Cereal Science
Lovegrove A
(2009)
Establishing substantial equivalence: proteomics.
in Methods in molecular biology (Clifton, N.J.)
| Description | Identified key genes encoding enzymes of arabinoxylan synthesis in wheat and demonstrated their functions and effects on AX structure and amount by transgenesis Identified beta-glucan synthase genes in wheat and demonstrated effects on glucan structure and size by transgenesis Identified genetic sources of high arabinoxylan fibre in wheat for exploitation in breeding |
| Exploitation Route | Projects have been developed with plant breeders to develop high and low fibre wheat for human health and biofuels/distilling, respectively |
| Sectors | Agriculture Food and Drink |