Structured Emulsions and Interfaces
Lead Research Organisation:
QUADRAM INSTITUTE BIOSCIENCE
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
The rising incidence of obesity demands that rational strategies be developed to reduce dietary fat and energy intake. We will facilitate the development of such strategies by investigating in vitro some key interfacial and colloidal mechanisms underlying the physicochemical behaviour of emulsified fats which may influence the perception of fats in emulsions and the digestion of fats in the GI tract. This will include the interactions with saliva in the mouth, interfacial mechanisms which control emulsion rheology (texture) and the interfacial processes which control lipid digestion. The new knowledge we will gain will enable new strategies to be developed to control dietary fat intake, for example by manipulation of interfacial behaviour to affect lipolysis which may then impact on satiety. We will achieve this by addressing the following objectives:
1. Describe the interfacial and molecular interaction processes responsible for the formation of the salivary mucous film, and identify structural changes resulting from interaction with food components.
2. Identify key colloidal and interfacial mechanisms in vitro that underpin the complex rheological properties of emulsions.
3. Define the key interfacial mechanisms that control the digestion of emulsions and subsequent transport of lipids and lipid soluble nutrients.
1. Describe the interfacial and molecular interaction processes responsible for the formation of the salivary mucous film, and identify structural changes resulting from interaction with food components.
2. Identify key colloidal and interfacial mechanisms in vitro that underpin the complex rheological properties of emulsions.
3. Define the key interfacial mechanisms that control the digestion of emulsions and subsequent transport of lipids and lipid soluble nutrients.
Planned Impact
unavailable
Organisations
- QUADRAM INSTITUTE BIOSCIENCE (Lead Research Organisation)
- Zhejiang University of Technology (Collaboration)
- QUALITY HEALTH LIMITED (Collaboration)
- Massey University (Collaboration)
- University of Warmia and Mazury in Olsztyn (Collaboration)
- University of Valencia (Collaboration)
- Teagasc (Collaboration)
- Swedish Oat Fiber AB (Collaboration)
- Autonomous University of Madrid (Collaboration)
- University of Leuven (Collaboration)
- Mondelez International (Collaboration)
- Marlow Foods (Collaboration)
- University of Leeds (Collaboration)
Publications
Husband F.A.
(2014)
The impact of the interfacial behaviour on emulsion rheology: A potential approach to reducing fat content in emulsified foods
in Gums and Stabilisers for the Food Industry 17 - The Changing Face of Food Manufacture: The Role of Hydrocolloids
Yablon, Dalia G.
(2014)
Scanning Probe Microscopy for Industrial Applications: Nanomechanical Characterization
Ash A
(2014)
Structural and compositional changes in the salivary pellicle induced upon exposure to SDS and STP
in Biofouling
Parker R
(2014)
The adsorption-desorption behaviour and structure function relationships of bile salts.
in Soft matter
Perez-Moral N
(2014)
Comparative study of the stability of multiple emulsions containing a gelled or aqueous internal phase
in Food Hydrocolloids
Ash A
(2014)
Structural characterisation of parotid and whole mouth salivary pellicles adsorbed onto DPI and QCMD hydroxyapatite sensors.
in Colloids and surfaces. B, Biointerfaces
Penfold R
(2015)
Enumerating and indexing many-body intramolecular interactions: a graph theoretic approach.
in Journal of mathematical chemistry
Torcello-Gomez Amelia
(2015)
Effect of substituent pattern and molecular weight of cellulose ethers on interactions with different bile salts
in FOOD & FUNCTION
| Description | The ability of bile salts to facilitate the digestion of fats was found to be dependent on the molecular structure of the bile salts. This affected the way they interacted with fats and solubilised the products of fat digestion and allow3d them to be adsorbed. Interfering with this process could allow us to develop foods that slowed down the digestion of fat in order to prolong appetite and thus allow individuals to manage food intake and weight gain. The structure and composition of bile salt micelles was found to be important for the digestion and absorption of fat soluble nutrients such as the flavanol quercetin, which is thought to have a range of health benefits. The interaction of quercetin with the micelles also controlled the uptake of quercetin into intestinal epithelial cells, and thus could affect bioavailability. Since then the research has moved onto more complex systems to understand the influence of other components such as phytosterols and dietary fibre on lipid digestion and bile salt handling in the gut. We have shown that the soluble dietary fibre can induce flocculation in emulsions and that more complex forms of the whole oat tissue which includes both soluble and insoluble fibre can slow down lipid digestion (Grundy et al. (2017) J. Funct. Food 38, 378-388). This highlights the contribution of the structural complexity of these ingredients to functionality, and further emphasises the importance of further research to understand the mechanisms underpinning the synergies between the different components and health impact. This is helping us to understand the mechanistic basis by which such foods can reduce hyperlipidaemia, prolong appetite and reduce serum cholesterol levels |
| Exploitation Route | The results will be taken forward into future research to understand the poor bioavailability of other lipophilic nutrients, like phytosterols, which are known to reduce cholesterol. We will also look at how other food components such as dietary fibre affect fat digestion, which could also explain their ability to reduce cholesterol levels and control appetite. The research has led to numerous grant proposals and projects, particularly with industry, where the research has generated interest in how to design foods with appetite supressing properties. |
| Sectors | Agriculture Food and Drink Healthcare Pharmaceuticals and Medical Biotechnology |
| Description | This work has led onto other grant applications looking into more general aspects of food structure digestion, and broader, fundamental studies of bile salt structure/function. The work on saliva has led to a successful; studentship, and two industrial funded projects worth ca £200k. Further grants have also been obtained from BBSRC through the DRINC and the Priming Food Partnerships initiatives in collaboration with academic partners (Imperial and Kings College London, Nottingham University) and involving food companies (Nestle, PepsiCo, Unilever, Iglo). Total research grants resulting from this project have totalled approximately £1m. The grant has also fed into recent GCRF applications, helping to develop links with Thailand, Mexico and Kenya. Research from this grant has also led to two further PhD studentships, one funded entirely by industry and the other through a BBSRC industry CTP. Recently, the work on bile salt binding to oat fibre has generated further interest. It has formed part of a recent studentship project (BBSRC CTP) which has revealed some of the mechanisms underpinning the functionality, which underpinned a recent BBSRC response more grant application. The work has led to a secondment to Kasetsart University in Thailand where we are discussing applications of the research with industrial partners to develop a mechanistic understanding of commercial food ingredients. The work has also led to several commercial projects through QIB Extra on protein and lipid digestion worth in excess of £100k |
| First Year Of Impact | 2018 |
| Sector | Agriculture, Food and Drink,Healthcare,Pharmaceuticals and Medical Biotechnology |
| Impact Types | Societal Economic |
| Description | BBSRC DRINC |
| Amount | £675,219 (GBP) |
| Funding ID | BB/N02110X/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2017 |
| End | 06/2019 |
| Description | BBSRC Priming Food Partnerships |
| Amount | £278,060 (GBP) |
| Funding ID | BB/P023924/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 06/2017 |
| End | 12/2018 |
| Description | Industry |
| Amount | £180,000 (GBP) |
| Organisation | Nestlé (Global) |
| Department | Société des Produits Nestlé SA |
| Sector | Private |
| Country | Switzerland |
| Start | 05/2015 |
| End | 05/2017 |
| Description | Mondelez CTP |
| Amount | £105,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2018 |
| End | 03/2022 |
| Description | Quorn Studentship |
| Amount | £105,000 (GBP) |
| Organisation | Marlow Foods |
| Sector | Private |
| Country | United Kingdom |
| Start | 01/2018 |
| End | 12/2021 |
| Description | Alan Mackie - University of Leeds |
| Organisation | University of Leeds |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Jointly supervised PhD studentship and joint contributions to papers |
| Collaborator Contribution | Jointly supervised PhD studentship and joint contributions to papers |
| Impact | Mulet-Cabero, A. I., A. R. Mackie, P. J. Wilde, M. A. Fenelon and A. Brodkorb (2019). "Structural mechanism and kinetics of in vitro gastric digestion are affected by process-induced changes in bovine milk." Food Hydrocolloids 86: 172-183. Ana-Isabel Mulet-Cabero: PhD student completed successfully, University of East Anglia, December 2018. Thesis title: Effect of Dairy Structures on Gastric Behaviour and Nutrient Digestion Kinetics using a Semi-Dynamic Model |
| Start Year | 2017 |
| Description | CIAL Madrid |
| Organisation | Autonomous University of Madrid |
| Country | Spain |
| Sector | Academic/University |
| PI Contribution | Hosted a visiting PhD student studying the digestibility and functional properties of pectin extracted from sunflower heads. Trained and supervised the student during a 3 month visit to QIB, contributed to submission of manuscript. |
| Collaborator Contribution | The student performed in vitro digestions and rheological measurements on samples of sunflower head pectin to understand the role of processing on functionality. |
| Impact | Munoz-Almagro, N., F. Rico-Rodriguez, P. J. Wilde, A. Montilla and M. Villamiel (2018). "Structural and technological characterization of pectin extracted with sodium citrate and nitric acid from sunflower heads." Electrophoresis 39(15): 1984-1992. Plus one more paper in preparation |
| Start Year | 2017 |
| Description | Dr Frederic Prothon |
| Organisation | Swedish Oat Fiber AB |
| Country | Sweden |
| Sector | Private |
| PI Contribution | Our research gave some mechanistic explanations on the positive effect of some oat products on lipid metabolism. |
| Collaborator Contribution | He provide some oat oils and oat bran. |
| Impact | We presented our data to various conferences and a manuscript is in preparation. |
| Start Year | 2017 |
| Description | Lipase inhibition |
| Organisation | Quality Health Limited |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Currently negotiating contract research on in vitro lipase inhibition |
| Collaborator Contribution | Bringing existing product and technical knowledge to the project |
| Impact | This partnership is ongoing, and no key outputs have been generated yet |
| Start Year | 2020 |
| Description | Mondelez |
| Organisation | Mondelez International |
| Country | United States |
| Sector | Private |
| PI Contribution | The research capabilities were discussed with Mondelez and potential projects for their CTP allocation were discussed. A project was developed and ultimately funded in 2017. The PhD student will start in April 2018 |
| Collaborator Contribution | The hold a BBSRC CTP award, and they helped develop the studentship project with us on determining the mechanisms underpinning the health benefits of fibre. |
| Impact | A PhD studentship was funded through the Mondelez CTP award, due to start in April 2018 |
| Start Year | 2016 |
| Description | Quorn |
| Organisation | Marlow Foods |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | The research was discussed in the context of the business of Marlow Foods (Quorn) to understand the mechanisms underpinning the digestion and bioavailability of proteins, fibre and other nutrients from Quorn products. After negotiations, Marlow Foods agreed to fund a PhD studentship to study these aspects. |
| Collaborator Contribution | Marlow Foods brought the problem in terms of a lack of a mechanistic understanding of the health benefits of Quorn. They contributed financially to the project by fully funding a PhD student (plus an additional student with Imperial College London). |
| Impact | The studentship only started in January 2018, so it is too early to say. |
| Start Year | 2017 |
| Description | Riddet Institute |
| Organisation | Massey University |
| Department | Riddet Institute |
| Country | New Zealand |
| Sector | Academic/University |
| PI Contribution | Co-supervising a student (Sewuese Okubanjo) based at the Riddet in New Zealand. Have provided advice and guidance on the course and outcomes of the project. |
| Collaborator Contribution | The work has been based in New Zealand and publications arising from the project will be initiated by the Riddet team, with my input. |
| Impact | Droplet-Stabilized Oil-in-Water Emulsions Protect Unsaturated Lipids from Oxidation. Sewuese S. Okubanjo, Simon M. Loveday, Aiqian Ye, Peter J. Wilde and Harjinder Singh. Journal of Agricultural and Food Chemistry. In press DOI: 10.1021/acs.jafc.8b02871 |
| Start Year | 2015 |
| Description | Tara Grauwet KU Leuven |
| Organisation | University of Leuven |
| Country | Belgium |
| Sector | Academic/University |
| PI Contribution | Technical expertise on in vitro lipid digestion and confocal microscopy |
| Collaborator Contribution | Funding from fellowship to visit and conduct collaborative research on the role pf pectin on lipid digestion. |
| Impact | Visits have been postponed due to the Covid-19 pandemic. Currently discussion activities and timelines for future visit. |
| Start Year | 2020 |
| Description | Teagasc |
| Organisation | Teagasc |
| Department | Teagasc Food Research Centre |
| Country | Ireland |
| Sector | Academic/University |
| PI Contribution | Joint supervision of 2 Walsh Fellowship PhD studentships, funded by Teagasc, and hosted partly at QIB and partly at Teagasc Food research Centres (1). Miss Ana-Isabel Mulet-Cabero supervised by Dr Andre Brodkorb at the Food Research Centre, Moorepark, Fermoy, Ireland (2). Mr Rares-Ionut Birsan, supervised by Dr Dilip Rai at The Ashtown Food Research Centre, Dublin, Ireland |
| Collaborator Contribution | Joint supervision of PhD students, hosted student while placed in Teagasc for 3.5 years in total between the two students |
| Impact | Mulet-Cabero, A. I., A. R. Mackie, P. J. Wilde, M. A. Fenelon and A. Brodkorb (2019). "Structural mechanism and kinetics of in vitro gastric digestion are affected by process-induced changes in bovine milk." Food Hydrocolloids 86: 172-183. Ana-Isabel Mulet-Cabero: PhD student completed successfully, University of East Anglia, December 2018. Thesis title: Effect of Dairy Structures on Gastric Behaviour and Nutrient Digestion Kinetics using a Semi-Dynamic Model |
| Start Year | 2014 |
| Description | University of Valencia - Prof M.J. Lagarda and Dr G. Garcia-Llatas |
| Organisation | University of Valencia |
| Department | Faculty of Pharmacy |
| Country | Spain |
| Sector | Academic/University |
| PI Contribution | We have a manuscript in preparation and presented the work at 2 conferences. |
| Collaborator Contribution | The group in Valencia taught us how to perform phytosterol analysis using GC. |
| Impact | We have a manuscript in preparation and presented the work at 2 conferences. |
| Start Year | 2017 |
| Description | University of Warmia and Mazury |
| Organisation | University of Warmia and Mazury in Olsztyn |
| Country | Poland |
| Sector | Academic/University |
| PI Contribution | Hosted visiting scientist Dr Marek Aljewicz for 3 months to perform experimental study on the interaction of beta glucans and milk proteins |
| Collaborator Contribution | They brought previous experimental results and data to aid the design of the experiments at QIB to support the submission of a manuscript (in preparation), and to contribute to the studies of the visitor's PhD student at UWM |
| Impact | The collaboration is still active. We expect the results will contribute to a joint paper. This is a multidisciplinary collaboration which includes mineral nutrition (bioavailability), microbiology and food physical chemistry. |
| Start Year | 2019 |
| Description | Zhejiang University of Technology, China |
| Organisation | Zhejiang University of Technology |
| Country | China |
| Sector | Academic/University |
| PI Contribution | We hosted Dr Jianhua Liu as a visiting scientist in my group for one year between August 2016 and August 2017, he received training in colloidal techniques and in vitro digestion methodologies. |
| Collaborator Contribution | Jianhua investigated the functionality of fish oil emulsions during his visit and wrote up two papers, one of which has been recently published |
| Impact | Liu, J. H., W. L. Liu, L. J. Salt, M. J. Ridout, Y. T. Ding and P. J. Wilde (2019). "Fish Oil Emulsions Stabilized with Caseinate Glycated by Dextran: Physicochemical Stability and Gastrointestinal Fate." Journal of Agricultural and Food Chemistry 67(1): 452-462. |
| Start Year | 2016 |
| Title | Foodstuffs having improved digestion properties |
| Description | Methods to increase the resistant starch content of peas through combined genomics and processing |
| IP Reference | GB2014104.0 |
| Protection | Patent application published |
| Year Protection Granted | 2020 |
| Licensed | No |
| Impact | The discovery has led to several industrial interactions to further develop the discovery in terms of aiding the development of new food ingredients. This has led to a UKRI super follow on fund application to accelerate exploitation, and specific confidential discussions with individual companies to potentially license the IP towards ingredient development. |
| Description | IFST Lecture Sept 2022 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited to give a public lecture to IFST (Institute of Food Science & Technology) on food structure, appetite and energy intake |
| Year(s) Of Engagement Activity | 2022 |
| Description | Invited member of Irish Department of Agriculture Food and the Marine grant evaluation panel |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited to sit as an international expert on an Irish Department of Agriculture Food and the Marine grant evaluation panel. Call: Food Institutional Research Measure |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://www.gov.ie/en/publication/ce553-research/#:~:text=DAFM%20Funded%20Research&text=Food%20Insti... |
| Description | Judging panel RSC Emerging Technologies Competition |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Member of Judging Panel for the Royal Society of Chemistry Emerging Technologies competition. Annual competition run by RSC to support start up companies. Food and Beverage panel. |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://www.rsc.org/competitions/emerging-technologies/previous-winners/ |
| Description | Judging panel RSC Emerging Technologies Competition 2021 |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Invited expert judge for the Royal Society of Chemistry Emerging Technologies competition - Food and Beverage panel |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://www.rsc.org/competitions/emerging-technologies/previous-winners |
| Description | Mail on Sunday article on fake foods |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Commented on a range of food myths that the article was trying to get a more balanced scientific view of, including the issue of coconut oil and health. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.dailymail.co.uk/health/article-6450123/How-fake-food-news-putting-risk.html |
| Description | Mycoprotein blog |
| Form Of Engagement Activity | Engagement focused website, blog or social media channel |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Educational blog focussed on recent publications about how mycoprotein influences digestion and promotes health effects |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://quadram.ac.uk/how-mycoprotein-influences-digestion-and-promotes-health-effects/ |
| Description | Norwich Science Festival |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Participated in Heroes of Food Research, highlighting the important role of human volunteers in dietary intervention studies. Discussed with the public the role and their contributions. Particularly our research involving saliva. |
| Year(s) Of Engagement Activity | 2016 |
| Description | Panel member of the EU JPI PREVNUT Call |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Invited evaluator and panel member of the EU JPI PREVNUT Call. Joint Programming Initiative 'A Healthy Diet for a Healthy Life' Evaluated and ranked proposals in the above call and prioritized the top proposals for funding. |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://www.healthydietforhealthylife.eu/index.php/call-activities/calls/98-calls-site-restyling/583... |
| Description | Presentation to Norwich Research Park Diet and Health Tea Club |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation of the food structure research to the Norwich Research Park Diet and Health Tea Club. This group is formed of researchers and clinicians from the Norwich Research Park, to disseminate and communicate developments in diet and health research and to foster collaborations. |
| Year(s) Of Engagement Activity | 2016 |
| Description | Public lecture to local members of the University of the Third Age |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | Public lecture organised for members of the University of the third age held at Quadram Institute, Norwich to disseminate and inform of the research activities and outputs of the Institute in the area of food structure and health. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Public lecture to the North Norfolk National Trust Society |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | A public lecture to the North Norfolk National Trust society to communicate some of the research activities of the Institute as part of our outreach activities. |
| Year(s) Of Engagement Activity | 2016 |
| Description | Summer Science Day, Halesworth |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Doleman J. F., Armah C. N., Kroon P., Wilde P., Mackie A., Vallins A.(2015) Heroes of Food Research: Summer Science Day at The Cut, Halesworth |
| Year(s) Of Engagement Activity | 2015 |
| Description | You tube video Food structure, colloid and digestion |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | You tube video describing the research done in the group to inform the wider public about the scope and impacts of our research and also to provide information to industry and other stakeholders who may want to collaborate |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.youtube.com/watch?v=e7aMpnps4-Q |