Response
Lead Research Organisation:
John Innes Centre
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
Genetic dissection of plant-microbe/pest interactions has led to the discovery of many key players in plant signal transduction. The challenge now is to link these components together, providing the detailed understanding of signal transduction mechanisms. A newly emergent area that we will focus on in the next 5 years is the cell-to-cell communication that allows the coordination of plant responses to pathogens, pests and symbionts. The technologies and understandings that we and others have developed over the last 5 years allow us to discriminate between the signal transduction mechanisms happening in different regions of the cell, in different cell types and at local versus systemic sites. Mathematical modelling will be incorporated with experimentation to explore these mechanisms of local and systemic signal propagation. There are several parallels between PAMP and symbiotic signaling and the work in this theme will allow us to compare and contrast these processes and to explore the mechanisms for differential activation of downstream responses.
PAMP-triggered immunity is associated with the production of a ROS burst, a calcium flux across the plasma membrane and the activation of a cytoplasmic kinase cascade. In contrast, symbiosis signaling involves oscillations of calcium in and around the nucleus that act as the primary mechanism of signal transduction. It is clear that ROS and calcium play important roles in intracellular signaling, but they also function in intercellular signaling. Differentiating between these local versus systemic responses is possible with better tools for measuring calcium and ROS changes.
PAMP-triggered immunity is associated with the production of a ROS burst, a calcium flux across the plasma membrane and the activation of a cytoplasmic kinase cascade. In contrast, symbiosis signaling involves oscillations of calcium in and around the nucleus that act as the primary mechanism of signal transduction. It is clear that ROS and calcium play important roles in intracellular signaling, but they also function in intercellular signaling. Differentiating between these local versus systemic responses is possible with better tools for measuring calcium and ROS changes.
Planned Impact
unavailable
Organisations
- John Innes Centre (Lead Research Organisation)
- Eberhard Karls University of Tübingen (Collaboration)
- SESVanderhave (Collaboration)
- University of Hertfordshire (Collaboration)
- University of North Carolina at Chapel Hill (Collaboration)
- Agriculture and Forestry University (Collaboration)
- French National Institute of Agricultural Research (Collaboration)
- UNIVERSITY OF EAST ANGLIA (Collaboration)
- The Sainsbury Laboratory (Collaboration)
- Ohio State University (Collaboration)
- University of Zurich (Collaboration)
- École normale supérieure de Lyon (ENS Lyon) (Collaboration)
- JOHN INNES CENTRE (Collaboration)
- Sichuan Agricultural University (Collaboration)
- G'S FRESH LIMITED (Collaboration)
- Center for Excellence in Molecular Plant Sciences (Collaboration)
- Plant Response Biotech S.L. (Collaboration)
- University of Amsterdam (Collaboration)
- ARLABION LTD (Collaboration)
- University of Adelaide (Collaboration)
- University of Geneva (Collaboration)
- KWS Saat (Collaboration)
Publications
Kamoun S
(2023)
Embracing the journey - Avoid being an energy vampire
Kamoun S
(2022)
When to preprint?
Kehr J
(2022)
Long-Distance Transported RNAs: From Identity to Function.
in Annual review of plant biology
Kelner A
(2018)
Dual Color Sensors for Simultaneous Analysis of Calcium Signal Dynamics in the Nuclear and Cytoplasmic Compartments of Plant Cells.
in Frontiers in plant science
Kunwar S.
(2017)
Managing bacterial wilt disease of tomato in open field conditions by improving host resistance through transgenic approach
in PHYTOPATHOLOGY
Lammertz M
(2019)
Widely Conserved Attenuation of Plant MAMP-Induced Calcium Influx by Bacteria Depends on Multiple Virulence Factors and May Involve Desensitization of Host Pattern Recognition Receptors.
in Molecular plant-microbe interactions : MPMI
Latorre SM
(2023)
Genomic surveillance uncovers a pandemic clonal lineage of the wheat blast fungus.
in PLoS biology
Leitão N
(2019)
Nuclear calcium signatures are associated with root development.
in Nature communications
| Title | Video showing how aphids sense aphid attack |
| Description | We launched a short video showing the method by which we can monitor the calcium elevations that occur in a leaf during aphid probing. |
| Type Of Art | Film/Video/Animation |
| Year Produced | 2018 |
| Impact | The video will be understood by a wide audience. |
| URL | https://www.youtube.com/watch?v=CK2iIPfcnvo |
| Description | Objective 2.1: We identified key components of the calcium signal machinery in response to aphid feeding. We demonstrated the interplay of the key components: the co-receptor BAK1, the plasma membrane ion channels GLR3.3 and GLR3.6, and the vacuolar calcium channel TPC1, thus linking signal recognition with response and signal transduction. In identifying core elements common to responses to a range of organisms, this study opens opportunities for developing an integrated understanding of plant responses to biotic stress. We have further narrowed this down to a critical reliance of wound-triggered responses on GLR3.3. We also developed a mathematical model for long-distance electrical signalling that occurs after wounding of a plant, eg during herbivory. This model predicts a wound-induced flow within the xylem that carries a wound substance that can initiate a calcium wave. We have experimentally confirmed this model (see Obj 2.2). We have developed a computational framework for simulating, analysing and quantifying how different calcium signals (during plant-microbe interactions) can differentially active different sets of calcium decoders. Calcium signalling is key to establishing symbiotic interactions and we generated a novel calcium reporter to visualise the calcium signal repartition between the cytoplasm and the nucleoplasm during this process. Using this new calcium reporter, we observed that symbiosis-induced nuclear calcium is released first into the nucleoplasm before diffusing to the cytoplasm identifying spatiotemporal details of this response. We found that the calcium channels CNGC15s are regulated by the calcium-bound form of the calmodulin 2 (holo-CaM2), which, upon release of calcium, provides negative feedback to close the CNGC15s. Combining structural and evolutionary analyses of CaM residues with bioinformatic analysis, we engineered a holo-CaM2 with an increased affinity for CNGC15s. This accelerates the calcium oscillation frequency and enhances root nodule symbiosis. During symbiotic relationships with plants, fungi channel inorganic and micronutrients from soil to the plant, and the plant supplies the fungi with organic nutrients. We demonstrated that as part of this exchange, the plant supplies lipids to its symbiotic fungi, thus providing the fungi with a robust source of carbon for their metabolic needs. This work shows that in addition to sugars, lipids are a major source of organic carbon delivered to the fungus, and this is necessary for production of fungal lipids. Plant responses to microbes involve an array of gene expression changes. We have studied the role of chromatin remodelling in defense gene induction and found that the SWR1 chromatin remodelling complex and H2A.Z are essential for mounting full transcriptional response and for conferring resistance. To explore spatio-temporal aspects of tissue responses, we further optimized low-input RNA-seq (LORIS) methods to characterize the temporal and spatial dynamics of transcriptome changes that occur in and around aphid stylet penetration sites of Arabidopsis leaves. In the context of chitin responses, we have established that whether plasmodesmata are closed or not regulates the amplitude and duration of the transcriptional response. With respect to long distance mRNA mobility, we demonstrated that a key transport protein involved in the movement of mRNA undergoes liquid-liquid phase separation in the phloem. We have determined that there is interaction between immune responses mediated by cell-surface receptors and those mediated by intracellular receptor such that these processes mutually potentiate each other. We have further characterised that cell-surface triggered signalling involves phosphorylation of the calcium channel OSCA1.3 by the cytoplasmic kinase BIK1. This signalling event is required for stomatal closure in response to bacterial pathogen perception. Similarly, we also investigated the specific signalling cascades associated with stomatal responses to chitin and have characterised specific phosphorylation signatures of the SLAH3 anion channel that mediate defence against the necrotroph Botrytis cinerea. We have determined several new components of the signalling processes that are triggered when plants perceive microbes. We determined that there are common elements to the accommodation of pathogenic and symbiotic fungi, specifically that Mildew Resistance locus O (MLO) is required not only for powdery mildew resistance but also for symbiotic associations with arbuscular mycorrhiza. We have used phosphoproteomics to define targets of the Pmk1 MAP kinase-dependent cell invasion pathway necessary for plasmodesmatal manipulation and tissue invasion by the rice blast fungus Magnaporthe oryzae. Further, using a forward genetic screen, we have identified regulators of effector gene expression in the rice blast fungus Magnaporthe oryzae. We have characterised one of these regulators, Rgs1, in detail. It is a known regulator of appressorium development but we have revealed that is also represses effector gene expression during the pre-penetration stage of development, prior to leaf tissue invasion. Objective 2.2: We identified that a calcium-responsive calmodulin-like protein (CML41) mediates flagellin-induced plasmodesmata closure. This protein is located at plasmodesmata, is calcium-responsive and positively regulates callose deposition. It links the regulation of cell-to-cell communication in response to bacteria to calcium signalling at plasmodesmata and presents a new tool from which to explore the role of cell-to-cell connectivity in immune responses. Our investigations into the mechanisms of control of cell-to-cell communication during immune responses revealed that chitin-triggered plasmodesmal closure is executed by a specific receptor complex involving the LysM receptors LYM2 and LYK4. The plasma membrane located receptor LYK5 is genetically required for plasmodesmal responses and acts upstream of plasmodesmal signalling. Plasmodesmata-specific signalling components that respond to both chitin and flg22 transduce a specific calcium dependent protein kinase-mediated, phosphorylation signature of the NADPH oxidase RBOHD, independent of the signature that executes plasma membrane chitin responses. We have identified two membrane domain proteins that regulate sorting of specific receptors to plasmodesmata, suggesting a mechanism for how plasmodesmal signalling is discrete from that in the plasma membrane. Linking specification of plasmodesmal membranes with the highly localised responses in them, we have identified members of two membrane domain families that are required for immune signalling at plasmodesmata and interact with plasmodesmal chitin receptors. We have also determined that chitin, flg22 and salicylic acid plasmodesmal signalling converges on a PDLP1-PDLP5-NHL3 complex that activates CALLOSE SYNTHASE1, identifying NHL3 as a novel immune regulator of plasmodesmal function. Further, we have identified that PAMP-triggered plasmodesmal closure is leaf age-dependent. To identify core and novel components of plasmodesmal signaling cascades, we generated two novel plasmodesmal proteomes - one from mature Arabidopsis leaves and one from the moss Physcomitrium. We used these datasets, as well as published datasets, to perform comparative phyloproteomics, which allowed us to identify key protein families that are conserved in plasmodesmata across the green lineage and contain proteins that function in plasmodesmal immune responses. To probe why plasmodesmal regulation during immune signalling is important, we developed a mathematical model for cell-cell communication during infection based on diffusion of a chemical agent cell to cell through the symplast. We have also developed a computational framework for studying cell-to-cell communication based on analytical solutions to the narrow escape problem. This framework will allow us to compute cell-to-cell movement as a function of plasmodesmal aperture and density. We have also identified that plasmodesmal closure has no impact on MAPK signalling but alters a PAMP-triggered ROS burst in a development-dependent manner. We made progress with characterizing the genetic components involved in the cell-to-cell spread of calcium waves that occur upon aphid feeding, and the study of whether these genetic components play similar roles incompatible and incompatible aphid-plant interactions. We have also continued to explore the mechanisms involved in the cell-to-cell spread of calcium bursts that occur upon aphid feeding and wounding. Three different aphid species induce Ca and glutamate bursts. We showed that an aphid effector suppresses plant Ca2+ bursts induced upon PRR-mediated perception of aphid peptide elicitors and DAMPs. We recently used quantitative imaging coupled with genetics and modelling to establish that wound-induced calcium waves travel via the same biophysical processes as calcium waves induced by salt stress and touch, and that calcium wave transmission in all contexts can be explained by diffusion and bulk flow of chemical messengers, which in the case of wounding are amino acids. We further determined that calcium waves do not mediate the induction of jasmonic acid responses. We have used LORIS to characterise spatiotemporal changes to the transcriptome that occur around aphid penetration sites. This identified that immune responses are executed in a co-ordinated manner in time and space surround a feeding site. We further identified that regulation of plasmodesmal aperture is required for the execution of chitin responses in distal tissues. We developed an exact Bayesian inference pipeline to analyse RNA-Seq data to detect the movement of RNA molecules upon wounding. The new methodology takes both experimental and software alignment uncertainty into account to compute SNP specific error rates, which coupled with a binomial likelihood and beta prior distribution can be solved analytically to deliver the posterior distribution for transport. The approach was validated exhaustively using simulated data and then applied to existing data sets. This development allow for the robust identification of RNA molecules that move upon grafting/wounding. We also developed mathematical models and associated software tools for analysing mRNA transport across graft-junctions. The methodology uses Bayesian inference for which we could derive exact solutions. We have developed a robust statistical analysis tool of the analysis of data cell to cell mobility data. This approach is superior to flawed analyses common used in the field and will have application across many fields of biological research. Further, we have developed a computational framework for studying cell to cell communication as a function of plasmodesmata geometry and distributions. This will allow us to assess the impact of perturbations to cell wall permeability (plasmodesmal parameters) on transport processes. We have used discovery proteomics to define fungal effectors that act at pit field sites that are used for cell-to-cell movement by the blast fungus during tissue invasion. These contribute to fungal virulence. We have also identified effectors from Colletotrichum higginsianum that target host plasmodesmata and manipulate their function. One of these effectors targets a plasmodesmata-located HMA domain protein int eh Arabidopsis host. Likewise, we have identified a group of effector proteins in the rice blast fungus that localise at pit field sites, which the fungus targets for cell-to-cell movement. These effectors are spatially and temporally regulated during cell invasion by M.oryzae. We have used a chemical genetic approach to identify MAP kinase-regulated genes associated with cell-to-cell invasion by the rice blast fungus. These are regulated at the transcriptional level or post-translationally. Using a combination of transcriptional profiling and phosphoproteomics, we are characterising their function and regulation. Objective 2.3: Using association genetics, we established that genetic loci affecting resistance in Brassica napus were determined by whether pathogens had necrotrophic or hemibiotrophic lifestyles. The results form the basis of discussions about collaborative research on brassica pathology with industry. We also performed genome-wide association studies (GWAS) with NLP-induced ROS on a Brassica napus diversity panel, and identified novel QTL associated with quantitative and qualitative responses. We are currently in the process of identifying the underlying genes and are using Arabidopsis to determine whether they contribute to disease resistance. Three Mlo homologues induced by mycorrhiza in barley have been identified and functional tests to investigate their role in mycorrhization are in progress. The results will improve understanding of the trade-off between disease resistance and mycorrhization in sustainable agriculture. We have demonstrated that recognition of Necrosis and Ethylene-inducing Like Peptides (NLPs) contributes to quantitative disease resistance in Brassica napus and have defined the genetic locus for this. We have also identified and mapped a novel gene locus conferring resistance to multiple races of downy mildew in Brassica oleracea and are now undertaking expression profiling to identify the causative gene. We identified a susceptibility factor for Light Leaf Spot disease of oilseed rape and are currently evaluating the potential for this to improve resistance using gene editing in collaboration with the University of Hertfordshire. We demonstrated that drought induces immune responses and disease resistance and defined nine gene clusters associated with this. The work identifies features of stability for more resilient disease resistance under changing environmental conditions. |
| Exploitation Route | This Theme of BBSRC's Institute Strategic Programme on Plant Health aims to determine the components and mechanisms by which plants transmit intra- and intercellular information in biotic interactions (pest, pathogen, symbiont). Understanding how plants carry out information signalling and processing is key for dissecting the molecular dialogue between plants and microbes and (in combination with outputs from the other themes) a first step towards the rational design of crop improvement for durable resistance and enhanced nutrient acquisition. |
| Sectors | Agriculture Food and Drink |
| Description | Interactions with industry included presentations at a science innovation showcase (JIC), a workshop on conservation and organic agriculture, formal meetings, a crop walk with RMI analytics and the grain supply industry, a keynote presentation for RMI analytics symposium and a podcast with Dewing Grain Ltd . The findings were used to develop collaborations for exchange with industry, specifically with CN Seeds Ltd and Sarba Shrestha Seeds Pvt in Nepal funded by partners and International Flexible Interchange Project (BBSRC BB/S018972/1). A consultancy was established with Arlabion Ltd on priming of immune responses. ICASE PhD projects were established with CN Seeds Ltd, New Heritage Barley Ltd and Syngenta. We have also established interactions with Syngenta and SESVanderHave which have primarily involved regular exchange of knowledge and methods. We have submitted patent applications with SESVanderHave and further had staff/student exchanges in both directions. We have established a major new collaboration with G's Fresh, a major producer of fresh vegetables for supermarkets, to investigate disease resistance in lettuce and other crops. Our work with vegetable breeders in Nepal has been included in the portfolio of projects in the newly established Norwich Institute for Sustainable Development (NISD). We obtained BBSRC Follow-on-Funding to create gene edited lines for the Light Leaf Spot susceptibility factor, working in collaboration with the University of Hertfordshire and KWS. New tools and resources for the research community: we generated and published new statistical methods for heteroskedastic data which is applicable across all biological data. We have carried out further plant health clinics in Kenya and Tanzania and disseminated worksheets and mobile phone apps through KALRO for blast disease diagnosis, control and pathogen surveillance. We have trained a new set of Plant Doctors using the CABI protocols, and these extension worked are working across Kenya and Tanzania in diagnostic work and pathogen surveillance, coordinated by KALRO and becA-ILRI in Nairobi. The research on phytoplasma SAP05 has generated several patents. In collaboration with Sophien Kamoun and Nick Talbot at TSL, we secured funding to hire an entrepreneur in residence who will help us to launch a start-up. Our work on theme 2.1 has led to novel partnerships with Limagrain/RAGT/KWS with the aim to translate our discovery into UK crops of agronomical importance. |
| First Year Of Impact | 2019 |
| Sector | Agriculture, Food and Drink,Communities and Social Services/Policy,Digital/Communication/Information Technologies (including Software),Education |
| Impact Types | Economic |
| Description | Project Leader of BRIGIT, a UK-wide consortium to mitigate the risks of Xylella fastidiosa outbreaks in the UK |
| Geographic Reach | Europe |
| Policy Influence Type | Membership of a guideline committee |
| Impact | The BRIGIT consortium includes people from various layers of government, charities, research institutes and industry. The writing of the BRIGIT proposal and activities within BRIGIT so far increased the knowledge of the consortium members about the Xylella pathosystem and how Xylella fastidiosa may spread in the UK and harm the environment. This is likely to influence future regulations to maximize protection of the UK environment. |
| URL | https://www.jic.ac.uk/brigit/ |
| Description | DTP iCASE |
| Amount | £100,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2017 |
| End | 09/2021 |
| Description | H2020-MSCA-IF-2019 |
| Amount | € 212,933 (EUR) |
| Funding ID | 891144 |
| Organisation | European Commission |
| Sector | Public |
| Country | Belgium |
| Start | 05/2020 |
| End | 05/2022 |
| Description | NRP-DTP |
| Amount | £79,108 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2020 |
| End | 09/2024 |
| Description | NRP-DTP |
| Amount | £79,108 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2018 |
| End | 09/2022 |
| Description | NRP-DTP |
| Amount | £79,108 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2019 |
| End | 09/2023 |
| Description | PhD Student Fellowship |
| Amount | £23,977 (GBP) |
| Organisation | Gatsby Charitable Foundation |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 09/2018 |
| End | 09/2022 |
| Description | Plamorf |
| Amount | € 6,000,000 (EUR) |
| Organisation | European Research Council (ERC) |
| Sector | Public |
| Country | Belgium |
| Start | 03/2019 |
| End | 04/2025 |
| Description | Plasmodesmata as regulators of the growth-defence trade-off |
| Amount | £549,881 (GBP) |
| Funding ID | BB/X007685/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 05/2023 |
| End | 03/2026 |
| Description | Quality Seed for Agriculture and Nutrition in Nepal |
| Amount | £58,417 (GBP) |
| Funding ID | BB/S018972/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2019 |
| End | 03/2020 |
| Title | Dual Color Sensors for Simultaneous Analysis of Calcium Signal Dynamics in the Nuclear and Cytoplasmic Compartments of Plant Cells |
| Description | Spatiotemporal changes in cellular calcium (Ca2+) concentrations are essential for signal transduction in a wide range of plant cellular processes. In legumes, nuclear and perinuclear-localized Ca2+ oscillations have emerged as key signatures preceding downstream symbiotic signaling responses. Förster resonance energy transfer (FRET) yellow-based Ca2+ cameleon probes have been successfully exploited to measure the spatiotemporal dynamics of symbiotic Ca2+ signaling in legumes. Although providing cellular resolution, these sensors were restricted to measuring Ca2+ changes in single subcellular compartments. In this study, we have explored the potential of single fluorescent protein-based Ca2+ sensors, the GECOs, for multicolor and simultaneous imaging of the spatiotemporal dynamics of cytoplasmic and nuclear Ca2+ signaling in root cells. Single and dual fluorescence nuclear and cytoplasmic-localized GECOs expressed in transgenic Medicago truncatula roots and Arabidopsis thaliana were used to successfully monitor Ca2+ responses to microbial biotic and abiotic elicitors. In M. truncatula, we demonstrate that GECOs detect symbiosis-related Ca2+ spiking variations with higher sensitivity than the yellow FRET-based sensors previously used. Additionally, in both M. truncatula and A. thaliana, the dual sensor is now able to resolve in a single root cell the coordinated spatiotemporal dynamics of nuclear and cytoplasmic Ca2+ signaling in vivo. The GECO-based sensors presented here therefore represent powerful tools to monitor Ca2+ signaling dynamics in vivo in response to different stimuli in multi-subcellular compartments of plant cells. |
| Type Of Material | Physiological assessment or outcome measure |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | the cytoplasmic and nucleoplasmic dual sensors developed in this work are powerful tools to analyze the subcellular Ca2+ dynamics between the cytoplasm and nucleus in planta. These sensors will unequivocally be of interest in exploring Ca2+-mediated signaling in response to diverse stimuli in different plant species. |
| URL | https://www.frontiersin.org/articles/10.3389/fpls.2018.00245/full |
| Title | Quantitative cell to cell mobility assay |
| Description | We generated dual expression, GoldenGate vectors for plant transformation that all for marking cells that are transformed and the production and tracking of a candidate cell-to-cell mobile fluorophore. We have also generated a suite of size standards that allow quantitative analysis of cell to cell mobility. This quantitative approach allows for the identification of hypermobility |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2021 |
| Provided To Others? | Yes |
| Impact | This tool has been distributed to a US lab and used in a screen for Liberibacter effectors |
| URL | https://doi.org/10.1101/2021.01.13.426415 |
| Title | Comparative phyloproteomics identifies conserved plasmodesmal proteins - Project PXD038964 |
| Description | This dataset contains raw mass spec data obtained from analysis of proteins in purified plasmodesmal fractions of Arabidopsis leaves and moss. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2023 |
| Provided To Others? | Yes |
| Impact | none to date |
| URL | https://www.ebi.ac.uk/pride/archive/projects/PXD038964 |
| Title | Diffusion and bulk flow of amino acids mediate calcium waves in plants |
| Description | We present time-lapse images of whole leaves or plants, collected from plants (wild-type and a range of mutants) expressing calcium and glutamate reporters. We used these images to define the dynamics of calcium and glutamate waves that emanate from the sites at which different stimuli were triggered. These images are available as .czi and the scripts we used to analyse them are available via zenodo as described in the links. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2022 |
| Provided To Others? | Yes |
| Impact | This dataset has been used by researchers at Cornell University in their own research |
| URL | https://www.ebi.ac.uk/biostudies/BioImages/studies/S-BIAD464?query=S-BIAD464 |
| Title | Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape (Brassica napus) |
| Description | Supplemental datasets associated with publication: Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape (Brassica napus) Abstract Crops are affected by several pathogens, but these are rarely studied in parallel to identify common and unique genetic factors controlling diseases. Broad-spectrum quantitative disease resistance (QDR) is desirable for crop breeding as it confers resistance to several pathogen species. Here, we use associative transcriptomics (AT) to identify candidate gene loci associated with Brassica napus QDR to four contrasting fungal pathogens: Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum. We did not identify any loci associated with broad-spectrum QDR to fungal pathogens with contrasting lifestyles. Instead, we observed QDR dependent on the lifestyle of the pathogen-hemibiotrophic and necrotrophic pathogens had distinct QDR responses and associated loci, including some loci associated with early immunity. Furthermore, we identify a genomic deletion associated with resistance to V. longisporum and potentially broad-spectrum QDR. This is the first time AT has been used for several pathosystems simultaneously to identify host genetic loci involved in broad-spectrum QDR. We highlight candidate loci for broad-spectrum QDR with no antagonistic effects on susceptibility to the other pathogens studies as candidates for crop breeding. Summary of data files Table S1 Mean, normalized phenotype data for resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by PAMPS (chitin, flg22, and elf18). Table S2 Full list of single nucleotide polymorphism (SNP) markers and significance levels from genome-wide association (GWA) analyses for resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by PAMPS (chitin, flg22, and elf18). Table S3 Full list of gene expression markers (GEMs) and significance levels from GEM analyses for resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by PAMPS (chitin, flg22, and elf18). Table S4 184 gene expression markers (GEMs) associated with chitin-induced ROS compared with GEMs associated with resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by flg22, and elf18. Lists correspond to Venn diagrams in Fig. 2. Table S5 Enrichment analyses to determine if the number of gene expression markers (GEMs) shared between different lists is greater than the number of GEMs that would be expected by chance (e.g., lists of quantitative disease resistance (QDR) GEMs for two fungal pathogens). Table S6 Results from Weighted Co-expression Gene Network Analysis. Significant modules, significant GEM markers within modules, and GO terms associated with the magenta and black modules are indicated. Table S7 Shared gene expression markers (GEMs) associated with resistance to different pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum). Lists correspond to matrices and Venn diagrams in Fig. 1. Table S8 List of genes in linkage disequilibrium with the top marker for Verticillium longisporum resistance from genome-wide association (GWA) analysis on chromosome A09, the homoeologous region on C08, and their query coverage in Brassica napus reference genotypes Extended description of data files Table S1 Mean, normalized phenotype data for resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by PAMPS (chitin, flg22, and elf18). These data were used for association transcriptomic analysis. Table S2 Full list of single nucleotide polymorphism (SNP) markers and significance levels from genome-wide association (GWA) analyses for resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by PAMPS (chitin, flg22, and elf18). Each excel tab contains the analyses for a single trait. The best fit model for GWA analysis is indicated in the tab title. Manhattan plots showing marker-trait association are included for data visualization; x-axis indicates SNP location along the chromosome; the y-axis indicates the -log10(p) (P value). Qqplots are included to demonstrate model fit. Table S3 Full list of gene expression markers (GEMs) and significance levels from GEM analyses for resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by PAMPS (chitin, flg22, and elf18). Each excel tab contains the analyses for a single trait. Manhattan plots showing marker-trait association are included for data visualization; x-axis indicates GEM location along the chromosome; the y-axis indicates the -log10(p) (P value). Table S4 184 gene expression markers (GEMs) associated with chitin-induced ROS compared with GEMs associated with resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by flg22, and elf18. Lists correspond to Venn diagrams in Fig. 2. The first tab includes all 184 GEMs associated with chitin-induced ROS. The subsequent tabs include lists of shared GEMs associated with chitin-induced ROS response and each additional trait (quantitative disease resistance (QDR) to each fungal pathogen or additional PAMP-induced ROS responses). The title of each tab indicates the data included in each comparison and the number of shared GEMs. Predicted Arabidopsis thaliana orthologs and corresponding descriptions are shown where possible. Table S5 Enrichment analyses to determine if the number of gene expression markers (GEMs) shared between different lists is greater than the number of GEMs that would be expected by chance (e.g., lists of quantitative disease resistance (QDR) GEMs for two fungal pathogens). The representation factor is the number of overlapping GEMs divided by the expected number of overlapping GEMs drawn from two independent groups (traits), considering the total number of GEMs sequenced (53884). A representation factor > 1 indicates more overlap than expected of two groups, a representation factor < 1 indicates less overlap than expected, and a representation factor of 1 indicates that the two groups by the number of genes expected for independent groups of genes. Table S6 Results from Weighted Co-expression Gene Network Analysis (WGCNA). The first tab indicates significant modules from WGCNA analysis. Black and magenta modules are associated with antagonistic effects on resistance/susceptibility to all four pathogens. The second tab includes a full list of the GEM markers (Table S3), which are in significant WGCNA modules. The third, fourth and, fifth tabs indicate all significant GEMs in the black module, GO terms associated with GEMs in the black module, and all GO terms associated with the black module, respectively. The sixth, seventh and, eighth tabs indicate all significant GEMs in the magenta module, GO terms associated with GEMs in the magenta module, and all GO terms associated with the magenta module, respectively. Table S7 Shared gene expression markers (GEMs) associated with resistance to different pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum). Lists correspond to matrices and Venn diagrams in Fig. 3. The first tab includes all GEMs associated quantitative disease resistance (QDR) to the fungal pathogens. The subsequent tabs include lists of shared GEMs associated with QDR to two or more fungal pathogens. The title of each tab indicates the data included in each comparison and the number of shared GEMs. Predicted Arabidopsis thaliana orthologs and corresponding descriptions are shown where possible. Table S8 List of genes in linkage disequilibrium with the top marker for Verticillium longisporum resistance from genome-wide association (GWA) analysis on chromosome A09 (107 genes)(Tab 1) and the homoeologous region on C08 (Tab 2). Their percentage identity and query coverage in Brassica napus reference genotypes Quinta, Tapidor, Westar and Zhongshuang 11 compared to the B. napus pantranscriptome is indicated. Predicted Arabidopsis thaliana orthologs and corresponding descriptions are shown where possible. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2023 |
| Provided To Others? | Yes |
| URL | https://zenodo.org/record/8321694 |
| Title | Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape (Brassica napus) |
| Description | Supplemental datasets associated with publication: Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape (Brassica napus) Abstract Crops are affected by several pathogens, but these are rarely studied in parallel to identify common and unique genetic factors controlling diseases. Broad-spectrum quantitative disease resistance (QDR) is desirable for crop breeding as it confers resistance to several pathogen species. Here, we use associative transcriptomics (AT) to identify candidate gene loci associated with Brassica napus constitutive QDR to four contrasting fungal pathogens: Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum. We did not identify any loci associated with broad-spectrum QDR to fungal pathogens with contrasting lifestyles. Instead, we observed QDR dependent on the lifestyle of the pathogen-hemibiotrophic and necrotrophic pathogens had distinct QDR responses and associated loci, including some loci associated with early immunity. Furthermore, we identify a genomic deletion associated with resistance to V. longisporum and potentially broad-spectrum QDR. This is the first time AT has been used for several pathosystems simultaneously to identify host genetic loci involved in broad-spectrum QDR. We highlight constitutively expressed candidate loci for broad-spectrum QDR with no antagonistic effects on susceptibility to the other pathogens studies as candidates for crop breeding. In conclusion, this study represents and advancement in our understanding if broad-spectrum QDR in B. napus and is a significant resource for the scientific community. Description of data files Full dataset for input into AT analysis Full datasets (infection phenotypes for A. brassicicola, B. cinerea, or V.longisporum, ROS measurements for chitin, flg22, or elf18) and link to original P. brassicae dataset. These datasets were used for input into the Associative Transcriptomics pipeline (Nichols, 2022, https://github.com/bsnichols/GAGA. https://zenodo.org/badge/latestdoi/512807075). Table S1 Mean, normalized phenotype data for resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by PAMPS (chitin, flg22, and elf18). These data were used for association transcriptomic analysis. Table S2 Full list of single nucleotide polymorphism (SNP) markers and significance levels from genome-wide association (GWA) analyses for resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by PAMPS (chitin, flg22, and elf18). Each excel tab contains the analyses for a single trait. The best fit model for GWA analysis is indicated in the tab title. Manhattan plots showing marker-trait association are included for data visualization; x-axis indicates SNP location along the chromosome; the y-axis indicates the -log10(p) (P value). Qqplots are included to demonstrate model fit. Table S3 Full list of gene expression markers (GEMs) and significance levels from GEM analyses for resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by PAMPS (chitin, flg22, and elf18). Each excel tab contains the analyses for a single trait. Manhattan plots showing marker-trait association are included for data visualization; x-axis indicates GEM location along the chromosome; the y-axis indicates the -log10(p) (P value). Table S4 184 gene expression markers (GEMs) associated with chitin-induced ROS compared with GEMs associated with resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by flg22, and elf18. Lists correspond to Venn diagrams in Fig. 2. The first tab includes all 184 GEMs associated with chitin-induced ROS. The subsequent tabs include lists of shared GEMs associated with chitin-induced ROS response and each additional trait (quantitative disease resistance (QDR) to each fungal pathogen or additional PAMP-induced ROS responses). The title of each tab indicates the data included in each comparison and the number of shared GEMs. Predicted Arabidopsis thaliana orthologs and corresponding descriptions are shown where possible. Table S5 Enrichment analyses to determine if the number of gene expression markers (GEMs) shared between different lists is greater than the number of GEMs that would be expected by chance (e.g., lists of quantitative disease resistance (QDR) GEMs for two fungal pathogens). The representation factor is the number of overlapping GEMs divided by the expected number of overlapping GEMs drawn from two independent groups (traits), considering the total number of GEMs sequenced (53884). A representation factor > 1 indicates more overlap than expected of two groups, a representation factor < 1 indicates less overlap than expected, and a representation factor of 1 indicates that the two groups by the number of genes expected for independent groups of genes. Table S6 Results from Weighted Co-expression Gene Network Analysis (WGCNA). The first tab indicates significant modules from WGCNA analysis. Black and magenta modules are associated with antagonistic effects on resistance/susceptibility to all four pathogens. The second tab includes a full list of the GEM markers (Table S3), which are in significant WGCNA modules. The third, fourth and, fifth tabs indicate all significant GEMs in the black module, GO terms associated with GEMs in the black module, and all GO terms associated with the black module, respectively. The sixth, seventh and, eighth tabs indicate all significant GEMs in the magenta module, GO terms associated with GEMs in the magenta module, and all GO terms associated with the magenta module, respectively. Table S7 Shared gene expression markers (GEMs) associated with resistance to different pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum). Lists correspond to matrices and Venn diagrams in Fig. 3. The first tab includes all GEMs associated quantitative disease resistance (QDR) to the fungal pathogens. The subsequent tabs include lists of shared GEMs associated with QDR to two or more fungal pathogens. The title of each tab indicates the data included in each comparison and the number of shared GEMs. Predicted Arabidopsis thaliana orthologs and corresponding descriptions are shown where possible. Table S8 List of genes in linkage disequilibrium with the top marker for Verticillium longisporum resistance from genome-wide association (GWA) analysis on chromosome A09 (107 genes)(Tab 1) and the homoeologous region on C08 (Tab 2). Their percentage identity and query coverage in Brassica napus reference genotypes Quinta, Tapidor, Westar and Zhongshuang 11 compared to the B. napus pantranscriptome is indicated. Predicted Arabidopsis thaliana orthologs and corresponding descriptions are shown where possible. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2023 |
| Provided To Others? | Yes |
| URL | https://zenodo.org/doi/10.5281/zenodo.10500446 |
| Title | Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape (Brassica napus) |
| Description | Supplemental datasets associated with publication: Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape (Brassica napus) Abstract Crops are affected by several pathogens, but these are rarely studied in parallel to identify common and unique genetic factors controlling diseases. Broad-spectrum quantitative disease resistance (QDR) is desirable for crop breeding as it confers resistance to several pathogen species. Here, we use associative transcriptomics (AT) to identify candidate gene loci associated with Brassica napus constitutive QDR to four contrasting fungal pathogens: Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum. We did not identify any loci associated with broad-spectrum QDR to fungal pathogens with contrasting lifestyles. Instead, we observed QDR dependent on the lifestyle of the pathogen-hemibiotrophic and necrotrophic pathogens had distinct QDR responses and associated loci, including some loci associated with early immunity. Furthermore, we identify a genomic deletion associated with resistance to V. longisporum and potentially broad-spectrum QDR. This is the first time AT has been used for several pathosystems simultaneously to identify host genetic loci involved in broad-spectrum QDR. We highlight constitutively expressed candidate loci for broad-spectrum QDR with no antagonistic effects on susceptibility to the other pathogens studies as candidates for crop breeding. In conclusion, this study represents and advancement in our understanding if broad-spectrum QDR in B. napus and is a significant resource for the scientific community. Description of data files Full dataset for input into AT analysis Full datasets (infection phenotypes for A. brassicicola, B. cinerea, or V.longisporum, ROS measurements for chitin, flg22, or elf18) and link to original P. brassicae dataset. These datasets were used for input into the Associative Transcriptomics pipeline (Nichols, 2022, https://github.com/bsnichols/GAGA. https://zenodo.org/badge/latestdoi/512807075). Table S1 Mean, normalized phenotype data for resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by PAMPS (chitin, flg22, and elf18). These data were used for association transcriptomic analysis. Table S2 Full list of single nucleotide polymorphism (SNP) markers and significance levels from genome-wide association (GWA) analyses for resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by PAMPS (chitin, flg22, and elf18). Each excel tab contains the analyses for a single trait. The best fit model for GWA analysis is indicated in the tab title. Manhattan plots showing marker-trait association are included for data visualization; x-axis indicates SNP location along the chromosome; the y-axis indicates the -log10(p) (P value). Qqplots are included to demonstrate model fit. Table S3 Full list of gene expression markers (GEMs) and significance levels from GEM analyses for resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by PAMPS (chitin, flg22, and elf18). Each excel tab contains the analyses for a single trait. Manhattan plots showing marker-trait association are included for data visualization; x-axis indicates GEM location along the chromosome; the y-axis indicates the -log10(p) (P value). Table S4 184 gene expression markers (GEMs) associated with chitin-induced ROS compared with GEMs associated with resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by flg22, and elf18. Lists correspond to Venn diagrams in Fig. 2. The first tab includes all 184 GEMs associated with chitin-induced ROS. The subsequent tabs include lists of shared GEMs associated with chitin-induced ROS response and each additional trait (quantitative disease resistance (QDR) to each fungal pathogen or additional PAMP-induced ROS responses). The title of each tab indicates the data included in each comparison and the number of shared GEMs. Predicted Arabidopsis thaliana orthologs and corresponding descriptions are shown where possible. Table S5 Enrichment analyses to determine if the number of gene expression markers (GEMs) shared between different lists is greater than the number of GEMs that would be expected by chance (e.g., lists of quantitative disease resistance (QDR) GEMs for two fungal pathogens). The representation factor is the number of overlapping GEMs divided by the expected number of overlapping GEMs drawn from two independent groups (traits), considering the total number of GEMs sequenced (53884). A representation factor > 1 indicates more overlap than expected of two groups, a representation factor < 1 indicates less overlap than expected, and a representation factor of 1 indicates that the two groups by the number of genes expected for independent groups of genes. Table S6 Results from Weighted Co-expression Gene Network Analysis (WGCNA). The first tab indicates significant modules from WGCNA analysis. Black and magenta modules are associated with antagonistic effects on resistance/susceptibility to all four pathogens. The second tab includes a full list of the GEM markers (Table S3), which are in significant WGCNA modules. The third, fourth and, fifth tabs indicate all significant GEMs in the black module, GO terms associated with GEMs in the black module, and all GO terms associated with the black module, respectively. The sixth, seventh and, eighth tabs indicate all significant GEMs in the magenta module, GO terms associated with GEMs in the magenta module, and all GO terms associated with the magenta module, respectively. Table S7 Shared gene expression markers (GEMs) associated with resistance to different pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum). Lists correspond to matrices and Venn diagrams in Fig. 3. The first tab includes all GEMs associated quantitative disease resistance (QDR) to the fungal pathogens. The subsequent tabs include lists of shared GEMs associated with QDR to two or more fungal pathogens. The title of each tab indicates the data included in each comparison and the number of shared GEMs. Predicted Arabidopsis thaliana orthologs and corresponding descriptions are shown where possible. Table S8 List of genes in linkage disequilibrium with the top marker for Verticillium longisporum resistance from genome-wide association (GWA) analysis on chromosome A09 (107 genes)(Tab 1) and the homoeologous region on C08 (Tab 2). Their percentage identity and query coverage in Brassica napus reference genotypes Quinta, Tapidor, Westar and Zhongshuang 11 compared to the B. napus pantranscriptome is indicated. Predicted Arabidopsis thaliana orthologs and corresponding descriptions are shown where possible. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2023 |
| Provided To Others? | Yes |
| URL | https://zenodo.org/doi/10.5281/zenodo.8321693 |
| Title | Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape (Brassica napus) |
| Description | Supplemental datasets associated with publication: Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape (Brassica napus) Abstract Crops are affected by several pathogens, but these are rarely studied in parallel to identify common and unique genetic factors controlling diseases. Broad-spectrum quantitative disease resistance (QDR) is desirable for crop breeding as it confers resistance to several pathogen species. Here, we use associative transcriptomics (AT) to identify candidate gene loci associated with Brassica napus constitutive QDR to four contrasting fungal pathogens: Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum. We did not identify any loci associated with broad-spectrum QDR to fungal pathogens with contrasting lifestyles. Instead, we observed QDR dependent on the lifestyle of the pathogen-hemibiotrophic and necrotrophic pathogens had distinct QDR responses and associated loci, including some loci associated with early immunity. Furthermore, we identify a genomic deletion associated with resistance to V. longisporum and potentially broad-spectrum QDR. This is the first time AT has been used for several pathosystems simultaneously to identify host genetic loci involved in broad-spectrum QDR. We highlight constitutively expressed candidate loci for broad-spectrum QDR with no antagonistic effects on susceptibility to the other pathogens studies as candidates for crop breeding. In conclusion, this study represents and advancement in our understanding if broad-spectrum QDR in B. napus and is a significant resource for the scientific community. Description of data files Full dataset for input into AT analysis Full datasets (infection phenotypes for A. brassicicola, B. cinerea, or V.longisporum, ROS measurements for chitin, flg22, or elf18) and link to original P. brassicae dataset. These datasets were used for input into the Associative Transcriptomics pipeline (Nichols, 2022, https://github.com/bsnichols/GAGA. https://zenodo.org/badge/latestdoi/512807075). Table S1 Mean, normalized phenotype data for resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by PAMPS (chitin, flg22, and elf18). These data were used for association transcriptomic analysis. Table S2 Full list of single nucleotide polymorphism (SNP) markers and significance levels from genome-wide association (GWA) analyses for resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by PAMPS (chitin, flg22, and elf18). Each excel tab contains the analyses for a single trait. The best fit model for GWA analysis is indicated in the tab title. Manhattan plots showing marker-trait association are included for data visualization; x-axis indicates SNP location along the chromosome; the y-axis indicates the -log10(p) (P value). Qqplots are included to demonstrate model fit. Table S3 Full list of gene expression markers (GEMs) and significance levels from GEM analyses for resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by PAMPS (chitin, flg22, and elf18). Each excel tab contains the analyses for a single trait. Manhattan plots showing marker-trait association are included for data visualization; x-axis indicates GEM location along the chromosome; the y-axis indicates the -log10(p) (P value). Table S4 184 gene expression markers (GEMs) associated with chitin-induced ROS compared with GEMs associated with resistance to pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum) and ROS response induced by flg22, and elf18. Lists correspond to Venn diagrams in Fig. 2. The first tab includes all 184 GEMs associated with chitin-induced ROS. The subsequent tabs include lists of shared GEMs associated with chitin-induced ROS response and each additional trait (quantitative disease resistance (QDR) to each fungal pathogen or additional PAMP-induced ROS responses). The title of each tab indicates the data included in each comparison and the number of shared GEMs. Predicted Arabidopsis thaliana orthologs and corresponding descriptions are shown where possible. Table S5 Enrichment analyses to determine if the number of gene expression markers (GEMs) shared between different lists is greater than the number of GEMs that would be expected by chance (e.g., lists of quantitative disease resistance (QDR) GEMs for two fungal pathogens). The representation factor is the number of overlapping GEMs divided by the expected number of overlapping GEMs drawn from two independent groups (traits), considering the total number of GEMs sequenced (53884). A representation factor > 1 indicates more overlap than expected of two groups, a representation factor < 1 indicates less overlap than expected, and a representation factor of 1 indicates that the two groups by the number of genes expected for independent groups of genes. Table S6 Results from Weighted Co-expression Gene Network Analysis (WGCNA). The first tab indicates significant modules from WGCNA analysis. Black and magenta modules are associated with antagonistic effects on resistance/susceptibility to all four pathogens. The second tab includes a full list of the GEM markers (Table S3), which are in significant WGCNA modules. The third, fourth and, fifth tabs indicate all significant GEMs in the black module, GO terms associated with GEMs in the black module, and all GO terms associated with the black module, respectively. The sixth, seventh and, eighth tabs indicate all significant GEMs in the magenta module, GO terms associated with GEMs in the magenta module, and all GO terms associated with the magenta module, respectively. Table S7 Shared gene expression markers (GEMs) associated with resistance to different pathogens (Alternaria brassicicola, Botrytis cinerea, Pyrenopeziza brassicae and Verticillium longisporum). Lists correspond to matrices and Venn diagrams in Fig. 3. The first tab includes all GEMs associated quantitative disease resistance (QDR) to the fungal pathogens. The subsequent tabs include lists of shared GEMs associated with QDR to two or more fungal pathogens. The title of each tab indicates the data included in each comparison and the number of shared GEMs. Predicted Arabidopsis thaliana orthologs and corresponding descriptions are shown where possible. Table S8 List of genes in linkage disequilibrium with the top marker for Verticillium longisporum resistance from genome-wide association (GWA) analysis on chromosome A09 (107 genes)(Tab 1) and the homoeologous region on C08 (Tab 2). Their percentage identity and query coverage in Brassica napus reference genotypes Quinta, Tapidor, Westar and Zhongshuang 11 compared to the B. napus pantranscriptome is indicated. Predicted Arabidopsis thaliana orthologs and corresponding descriptions are shown where possible. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2023 |
| Provided To Others? | Yes |
| URL | https://zenodo.org/doi/10.5281/zenodo.10499917 |
| Description | Agricluture and Forestry University collaboration in Nepal |
| Organisation | Agriculture and Forestry University |
| Country | Nepal |
| Sector | Academic/University |
| PI Contribution | Collaboration initiated on Yellow rust control in wheat, cabbage stem flea beertle in Brassicas and the agricultural supply chain in Nepal |
| Collaborator Contribution | Expertise in these subject areas as well as facilities for collaborative research at AFU |
| Impact | Too early to say |
| Start Year | 2020 |
| Description | Cell-to-cell mobility of effectors of Fusarium - Takken lab |
| Organisation | University of Amsterdam |
| Country | Netherlands |
| Sector | Academic/University |
| PI Contribution | We performed callose staining to assay for plasmodesmal function in effector-expressing plant lines |
| Collaborator Contribution | They provided all expertise and resources that relate to the pathosystem |
| Impact | No outcomes to date |
| Start Year | 2015 |
| Description | Characterization of the role of clade IV MLO in arbuscular mycorrhization |
| Organisation | Center for Excellence in Molecular Plant Sciences |
| Country | China |
| Sector | Academic/University |
| PI Contribution | We provided our expertise in arbuscular mycorrhiza phenotyping, mutant characterization and phylogenetic analysis. |
| Collaborator Contribution | The Ridout lab provided expertise in barley and wheat genetics and the Murray lab in transcriptomics analyses. |
| Impact | Publication: Mildew Locus O facilitates colonization by arbuscular mycorrhizal fungi in angiosperms. Jacott CN, Charpentier M, Murray JD, Ridout CJ. New Phytol. 2020 Jul;227(2):343-351. doi: 10.1111/nph.16465. |
| Start Year | 2017 |
| Description | Characterization of the role of clade IV MLO in arbuscular mycorrhization |
| Organisation | John Innes Centre |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We provided our expertise in arbuscular mycorrhiza phenotyping, mutant characterization and phylogenetic analysis. |
| Collaborator Contribution | The Ridout lab provided expertise in barley and wheat genetics and the Murray lab in transcriptomics analyses. |
| Impact | Publication: Mildew Locus O facilitates colonization by arbuscular mycorrhizal fungi in angiosperms. Jacott CN, Charpentier M, Murray JD, Ridout CJ. New Phytol. 2020 Jul;227(2):343-351. doi: 10.1111/nph.16465. |
| Start Year | 2017 |
| Description | Collaboration with Professor Xuewei Chen and Dr Min He, Sichuan Agricultural University, Chengdu |
| Organisation | Sichuan Agricultural University |
| Country | China |
| Sector | Academic/University |
| PI Contribution | A collaboration was established to study the molecular basis of septin aggregation and interaction with plasma membranes during appressorium development by the rice blast fungus Magnaporthe oryzae. We discovered a requirement for very long chain fatty acid synthesis (VLCFA) in septin aggregation and identified a compund that would inhibit this process. this was tested as a fungicide against rice blast in field trials. |
| Collaborator Contribution | The partners carried out extensive VLCFA tests on septin aggregation and identified the fungicidal compound, which we tested rigorously in my laboratory in independent tests. |
| Impact | A publication was generated describing this work He M, Su J, Xu Y, Chen J, Chern M, Lei M, Qi T, Wang Z, Ryder LS, Tang B, Osés-Ruiz M, Zhu K, Cao Y, Yan X, Eisermann I, Luo Y, Li W, Wang J, Yin J, Lam SM, Peng G, Sun X, Zhu X, Ma B, Wang J, Liu J, Qing H, Song L, Wang L, Hou Q, Qin P, Li Y, Fan J, Li D, Wang Y, Wang X, Jiang L, Shui G, Xia Y, Gong G, Huang F, Wang W, Wu X, Li P, Zhu L, Li S, Talbot NJ, Chen X. (2020) Discovery of broad-spectrum fungicides that block septin-dependent infection processes of pathogenic fungi. Nature Microbiol. 5(12):1565-1575. |
| Start Year | 2019 |
| Description | Collaboration with The Sainsbury Laboratory, Norwich |
| Organisation | The Sainsbury Laboratory |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Provision of aphid samples, (HPLC-purified) aphid extracts and plant pull downs to analyze by mass spectrometry. Contribution of aphid, microbial and plant sequence data to analyze the mass spectrometry data against. |
| Collaborator Contribution | Sample analyses with mass spectrometry and help with interpretation of the mass spectrometry data output. |
| Impact | - An improved understanding of the physical properties of aphid elicitors that induce PTI-like plant defense responses. |
| Start Year | 2018 |
| Description | Defence induction by Vial 8 |
| Organisation | Arlabion Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | We performed analysis of a novel compound developed by the company to investigate its role in defence induction for disease control. We produced a report that they used for a patent application |
| Collaborator Contribution | The partners provided the compound and advice about the nature of the chemical and the kind of analyses they required |
| Impact | A report that was used for the patent application |
| Start Year | 2018 |
| Description | Disease control in salad and vegetable crops |
| Organisation | G's Fresh Limited |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | We had initial discussions to develop a research collaboration to imporve disease control in vegetable and salad crops, which a particular concern for the comapny |
| Collaborator Contribution | They informed us of the nature of investigtion that they were interested in |
| Impact | We have submitted a proposal for the company to consider as a basis for establishing a collaborative reserch partnership |
| Start Year | 2020 |
| Description | ENS Lyon |
| Organisation | École normale supérieure de Lyon (ENS Lyon) |
| Country | France |
| Sector | Academic/University |
| PI Contribution | exchange of published and unpublished biomaterials, sharing of unpublished data |
| Collaborator Contribution | exchange of published and unpublished biomaterials, sharing of unpublished data |
| Impact | no output yet |
| Start Year | 2019 |
| Description | Formal research collaboration with SESVanderHave |
| Organisation | Sesvanderhave |
| Country | Belgium |
| Sector | Private |
| PI Contribution | My research group has provided advise on strategies to obtain aphid resistant sugar beet, exchanged knowledge on research progress in plant-insect interactions of the lab, and wrote BBRSC-LINK award to fund research. |
| Collaborator Contribution | SESVanderHave provides access to sugar beet breeding lines, genome sequence resources for these lines and insecticide-free field sites for collection of aphid populations in UK and Europe. They also funded a postdoctoral researcher in my group for one year, contributed 50% in-kind funds for the BBSRC-LINK award and funds a iCASE studentship in my group. PhD student Roland Wouters was recruited for the iCASE project. Roland is making good progress. |
| Impact | Generated knowledge on plant-insect interactions. Organized visits of the SESVanderHave team to JIC (two times per year) and my group at JIC to SESVanderHave headquarters in Tienen, Belgium (two times per year). Organized regular Skype calls to discuss research progress and ideas for future research. |
| Start Year | 2013 |
| Description | Improving Vegetable Crops in Nepal |
| Organisation | University of East Anglia |
| Department | School of International Development UEA |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Including the Nepal Project in the portfolio of projects in the new Norwich Institute for Sustainble Development |
| Collaborator Contribution | Our partners at UEA are contributing expertise in international development and business studies to address the research topic of improving vegetabe production in Nepal |
| Impact | Too early |
| Start Year | 2021 |
| Description | Improving resistnce to Light Leaf Spot in Brassica napus |
| Organisation | KWS Saat |
| Country | Germany |
| Sector | Private |
| PI Contribution | We had discussions about a collaborative project and wrote a propoal that is currently being evaluated by UKRI/BBSRC |
| Collaborator Contribution | They contributed expertise and would provide resources if the project is funded |
| Impact | Too early for any outputs |
| Start Year | 2020 |
| Description | Improving resistnce to Light Leaf Spot in Brassica napus |
| Organisation | University of Hertfordshire |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We had discussions about a collaborative project and wrote a propoal that is currently being evaluated by UKRI/BBSRC |
| Collaborator Contribution | They contributed expertise and would provide resources if the project is funded |
| Impact | Too early for any outputs |
| Start Year | 2020 |
| Description | Medicago LINC complexes function in nuclear morphology, nuclear movement, and root nodule symbiosis |
| Organisation | Ohio State University |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Supervision of the study Symbioses experiments |
| Collaborator Contribution | Study of the LINC components |
| Impact | Newman-Griffis AH, Del Cerro P, Charpentier M, Meier I. Medicago LINC complexes function in nuclear morphology, nuclear movement, and root nodule symbiosis. Plant Physiology. 2019. 179(2):491-506 |
| Start Year | 2016 |
| Description | Plant Response Biotech |
| Organisation | Plant Response Biotech S.L. |
| Country | Spain |
| Sector | Private |
| PI Contribution | This is an iCASE studentship with Plant Response Biotech. We have generated transgenic tomato homozygous lines expressing individual Arabidopsis receptor kinases belonging to the sub-family XII of LRR-RLKs. We have generated single and multiple insertional Arabidopsis mutants for LRR-RLK sub-family XII members. |
| Collaborator Contribution | The lines generated above will be tested at the end of year at Plant Response Biotech by the student employed in this project for disease resistance. The partner will provide lab space, consumable costs, access to growth chambers, access to relevant pathogens, and access to proprietary plant-derived and microbial elicitors. |
| Impact | not applicable yet. |
| Start Year | 2015 |
| Description | Plant stimulants to prime plant immunity |
| Organisation | Arlabion Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | We provided our expertise to address a specific research question for the Company |
| Collaborator Contribution | They provided the background and some technical knowledge to the project |
| Impact | A report has been submitted to the company |
| Start Year | 2017 |
| Description | Simultaneous Analysis of Calcium Signal Dynamics in the Nuclear and Cytoplasmic Compartments of Plant Cells |
| Organisation | French National Institute of Agricultural Research |
| Department | INRA Toulouse |
| Country | France |
| Sector | Public |
| PI Contribution | We generated the Dual calcium sensor and the stably transformed plants (Arabidopsis thaliana and Medicago truncatula) We performed the research in Arabidopsis thaliana |
| Collaborator Contribution | The Carvalho-Niebel lab generated DUAL sensor and stably transformed Medicago truncatula Performed the experiment in Medicago truncatula |
| Impact | Kelner A, Leitao N, Chabaud M, Charpentier M*, De Carvalho-Niebel F*. Dual Color Sensors for Simultaneous Analysis of Calcium Signal Dynamics in the Nuclear and Cytoplasmic Compartments of Plant Cells. Frontiers in Plant Science. 2018. 9:245. |
| Start Year | 2017 |
| Description | The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity |
| Organisation | University of Zurich |
| Country | Switzerland |
| Sector | Academic/University |
| PI Contribution | We provided our expertise in ion channel, calcium recording and use of heterologous system to assess calcium permeability. |
| Collaborator Contribution | The Zipfel lab identified the OSCA1.3 ion channel as a target phosphorylated by BIK1 and characterized its role in plant stomatal immunity |
| Impact | Publication: The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity. Thor K, Jiang S, Michard E, George J, Scherzer S, Huang S, Dindas J, Derbyshire P, Leitão N, DeFalco TA, Köster P, Hunter K, Kimura S, Gronnier J, Stransfeld L, Kadota Y, Bücherl CA, Charpentier M, Wrzaczek M, MacLean D, Oldroyd GED, Menke FLH, Roelfsema MRG, Hedrich R, Feijó J, Zipfel C. Nature. 2020 Sep;585(7826):569-573. doi: 10.1038/s41586-020-2702-1. |
| Start Year | 2017 |
| Description | The role of CML41 in flagellin induced plasmodesmata closure |
| Organisation | University of Adelaide |
| Department | School of Agriculture, Food and Wine |
| Country | Australia |
| Sector | Academic/University |
| PI Contribution | We provide expertise and technologies on the analysis of plasmodesmata in response to pathogens, live cell imaging and image analysis |
| Collaborator Contribution | The Gilliham lab identified CML41 and performed gene expression analysis |
| Impact | Publication: Xu B, Cheval C, Laohavisit A, Hocking B, Chiasson D, Olsson TSG, Shirasu K, Faulkner C, Gilliham M A calmodulin-like protein regulates plasmodesmal closure during bacterial immune responses. New Phytol. 2017 Jul;215(1):77-84. doi: 10.1111/nph.14599. Epub 2017 May 17. |
| Start Year | 2015 |
| Description | UNC |
| Organisation | University of North Carolina at Chapel Hill |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | exchange of published and unpublished biomaterials; sharing of unpublished data |
| Collaborator Contribution | exchange of published and unpublished biomaterials; sharing of unpublished data |
| Impact | no output yet |
| Start Year | 2019 |
| Description | University of Geneva |
| Organisation | University of Geneva |
| Department | Faculty of Sciences |
| Country | Switzerland |
| Sector | Academic/University |
| PI Contribution | exchange of published and unpublished biomaterials, sharing of unpublished data |
| Collaborator Contribution | exchange of published and unpublished biomaterials, sharing of unpublished data |
| Impact | no outputs yet |
| Start Year | 2019 |
| Description | University of Tuebingen |
| Organisation | Eberhard Karls University of Tübingen |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | exchange of published and unpublished biomaterials, sharing of unpublished data |
| Collaborator Contribution | exchange of published and unpublished biomaterials, sharing of unpublished data |
| Impact | stil no outputs |
| Start Year | 2019 |
| Title | Bootstrap analysis tool for non-normal biological data |
| Description | Phytologists rely on experimentally perturbing plants and monitoring the responses. Frequentist statistics are used to ascertain the probability that an observed difference between conditions was due to chance (a p value). When data are not normal and have differing variances, we propose data sets are better analysed by a bootstrap method that tests the null hypothesis that means (or medians) are the same between two conditions, instead of the commonly used Mann-Whitney-Wilcoxon test. We illustrate this with data from the cell-to-cell movement of GFP through plasmodesmata. We found that that with hypothetical distributions similar to cell-to-cell movement data, the Mann-Whitney-Wilcoxon produces a false positive rate of 17% while the bootstrap method maintains a false positive at the set rate of 5% under the same circumstances. Here we present this finding, as well as our rationale, an explanation of the bootstrap method and an R script for easy use. We have further demonstrated its use on published datasets from independent laboratories. |
| Type Of Technology | Software |
| Year Produced | 2020 |
| Open Source License? | Yes |
| Impact | none to date |
| Title | PyEscape: A narrow escape problem simulator package for Python |
| Description | We provide routines for running simulations of cell to cell movement by diffusion through plasmodesmata. |
| Type Of Technology | Software |
| Year Produced | 2020 |
| Open Source License? | Yes |
| Impact | This software allows for different geometries with various plasmodesmal arrangements to be investigated for their impact on cell to cell communication. |
| URL | https://joss.theoj.org/papers/10.21105/joss.02072 |
| Title | Studying the spatial-temporal progression of a response to evaluate dependency on diffusion |
| Description | A script to study the spatial-temporal progression of a response to evaluate dependency on diffusion |
| Type Of Technology | Software |
| Year Produced | 2022 |
| Open Source License? | Yes |
| Impact | none to date |
| URL | https://doi.org/10.5281/zenodo.6585006 |
| Title | faulknerfalcons/360_radial_scan: Toolkit for radial scan of responses with radial symmetry around a 360 angle |
| Description | scanning a circularly symmetrical response around a 360 degree angle |
| Type Of Technology | Software |
| Year Produced | 2022 |
| Open Source License? | Yes |
| Impact | none to date |
| URL | https://zenodo.org/record/6584998 |
| Title | faulknerfalcons/CF_peaks_detection: Analysis of systemic CF spread in adult leaves |
| Description | Analysis of systemic CF spread in adult leaves of Arabidopsis via a peak and valleys detection mechanism |
| Type Of Technology | Software |
| Year Produced | 2022 |
| Open Source License? | Yes |
| Impact | none to date |
| URL | https://zenodo.org/record/6585010 |
| Title | faulknerfalcons/Cheval-2020-PNAS: Analysis Scripts for Cheval, Samwald, Johnston, et al., 2020, PNAS |
| Description | Chitin perception in plasmodesmata characterizes submembrane immune-signaling specificity in plants Cheval, Samwald, Johnston, et al., 2020, PNAS, DOI: 10.1073/pnas.1907799117 A collection of R scripts to exemplify how we analysed our bombardment and FRAP data in the manuscript. Contents Combinatorics statistics R script (.R) FRAP Example raw data (.csv) Example decay curves (.csv) Example sanity checking figures (.png) R scripts to analyse data (.R) Sample data provided by Samwald, S (https://orcid.org/0000-0003-4401-8862). Raw data, collated by figure, for use with the combinatorics script is available as a supplemental file at PNAS (distributed under Creative Commons Attribution License 4.0 (CC BY)). |
| Type Of Technology | Software |
| Year Produced | 2020 |
| Open Source License? | Yes |
| URL | https://zenodo.org/record/3727532 |
| Title | faulknerfalcons/Cheval-2020-PNAS: Analysis Scripts for Cheval, Samwald, Johnston, et al., 2020, PNAS |
| Description | Chitin perception in plasmodesmata characterizes submembrane immune-signaling specificity in plants Cheval, Samwald, Johnston, et al., 2020, PNAS, DOI: 10.1073/pnas.1907799117 A collection of R scripts to exemplify how we analysed our bombardment and FRAP data in the manuscript. Contents Combinatorics statistics R script (.R) FRAP Example raw data (.csv) Example decay curves (.csv) Example sanity checking figures (.png) R scripts to analyse data (.R) Sample data provided by Samwald, S (https://orcid.org/0000-0003-4401-8862). Raw data, collated by figure, for use with the combinatorics script is available as a supplemental file at PNAS (distributed under Creative Commons Attribution License 4.0 (CC BY)). |
| Type Of Technology | Software |
| Year Produced | 2020 |
| Open Source License? | Yes |
| URL | https://zenodo.org/record/3727531 |
| Title | faulknerfalcons/Costum_radial_scan_with-central-exclusion: Toolkit for radial scan of radial responses across a defined angle |
| Description | Toolkit for scanning and analysis of radial responses across a defined angle, allowing for exclusion of a cenral area |
| Type Of Technology | Software |
| Year Produced | 2022 |
| Open Source License? | Yes |
| Impact | none to date |
| URL | https://zenodo.org/record/6585004 |
| Title | faulknerfalcons/Johnston-2020-Bootstrap: Scripts and data for Johnston and Faulkner 'A bootstrap approach is a superior statistical method for the comparison of non-normal data with differing variances.' |
| Description | A bootstrap approach is a superior statistical method for the comparison of non-normal data with differing variances. Johnston and Faulkner, New Phytologist (2020) doi: 10.1111/nph.17159 Scripts for using our proposed bootstrap method: medianBootstrap.R contains functions to replace wilcox.test() with medianBootstrap() medianBootstrapToolbox.R contains an extended set of functions, allowing for the plotting of the null distribution, replacement of one-way ANOVAs, and a meanBootstrap(). A primer on how to use the medianBootstrap script is provided as a webpage (medianBootstrap.html) and R markdown (medianBootstrap.Rmd). All the scripts and data used within the manuscript and figures used under a CC BY 4.0 licence. |
| Type Of Technology | Software |
| Year Produced | 2020 |
| Open Source License? | Yes |
| URL | https://zenodo.org/record/4326967 |
| Title | faulknerfalcons/Johnston-2020-Bootstrap: Scripts and data for Johnston and Faulkner 'A bootstrap approach is a superior statistical method for the comparison of non-normal data with differing variances.' |
| Description | A bootstrap approach is a superior statistical method for the comparison of non-normal data with differing variances. Johnston and Faulkner, New Phytologist (2020) doi: 10.1111/nph.17159 Scripts for using our proposed bootstrap method: medianBootstrap.R contains functions to replace wilcox.test() with medianBootstrap() medianBootstrapToolbox.R contains an extended set of functions, allowing for the plotting of the null distribution, replacement of one-way ANOVAs, and a meanBootstrap(). A primer on how to use the medianBootstrap script is provided as a webpage (medianBootstrap.html) and R markdown (medianBootstrap.Rmd). All the scripts and data used within the manuscript and figures used under a CC BY 4.0 licence. |
| Type Of Technology | Software |
| Year Produced | 2020 |
| Open Source License? | Yes |
| URL | https://zenodo.org/record/4326968 |
| Title | faulknerfalcons/Linear_progression: Toolkit to study the progression of a response along a liner path |
| Description | No description provided. |
| Type Of Technology | Software |
| Year Produced | 2022 |
| Open Source License? | Yes |
| Impact | None to date |
| URL | https://zenodo.org/record/6585016 |
| Title | faulknerfalcons/Transects_Scan: Toolkit to study the progression of a response via scanning along perpendicular transects |
| Description | No description provided. |
| Type Of Technology | Software |
| Year Produced | 2022 |
| Open Source License? | Yes |
| Impact | none to date |
| URL | https://zenodo.org/record/6585014 |
| Description | A taste of Genetic Diversity: demonstration activity for the John Innes Centre open day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | A demonstration of our research was performed at an open day at the John Innes Centre, involving hands on activities and discussion on the theme of 'a taste of genetic diversity. The activities involved showing how brassicas were used in baby leaf salads, and how heritage barley varieties were used in brewing beer |
| Year(s) Of Engagement Activity | 2017 |
| Description | Advances - The john Innes Centre magazine |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | Getting to the root of agricultural pollution. |
| Year(s) Of Engagement Activity | 2021 |
| Description | Agri-tech: Science in Sustainable Agriculture |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | The talk describe the urgency to shift our agricultural practice and reduce chemical fertilizer use. The talk was well received and opened new connection with industries. |
| Year(s) Of Engagement Activity | 2021 |
| Description | Annals of Botany Lecturer - Australian Society of Plant Science Meeting |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | I was the Annals of Botany lecturer at the ASPS symposium. I presented a research talk. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Annual Science Meeting for general audience organised by the research and support Staff Voice |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | The talk was well attended, with 40 people attending in person and 172 registrations in total. Four local (Norfolk) schools attended online including Years 10 and 13 students from Wymondham College, Notre Dame, Sprowston High and Sheringham High. There were also 20 people from TSL and Youth STEMM and feedback from the schools was very positive. The hybrid format had been very successful. It is a great way to introduce school students to JIC science. |
| Year(s) Of Engagement Activity | 2021 |
| Description | Attendance and presentation at Sensors in Food and Agriculture 2018 meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Field testing and the research underpinning the soil sensor development was reported at the Sensors in Food and Agriculture 2018 meeting. At this meeting an industrial partner was identified who has relevant expertise to commercially develop the soil sensor technology. |
| Year(s) Of Engagement Activity | 2018 |
| URL | http://www.rsc.org/events/detail/33486/sensors-in-food-and-agriculture-2018 |
| Description | BBC Farming Today Interview 25th October 2018 |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Interview for BBC Farming Today on the CJEU ruling on Genome Editing as a form of genetic modification, subject to the same regulation as transgenic crops, and the likely effect of this ruling on agricultural innovation in Europe. The interview covered genome editing technologies, the potential for crop improvement, the details of the ruling, the contrast with other international jurisdications, and the potential effect on agriculture in Europe. |
| Year(s) Of Engagement Activity | 2018 |
| Description | BBC Look East Interview |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | I was interviewed about the potential impact of Brexit on scientific research at The Sainsbury Laboratory and the Norwich Research Park. The interview covered the type of research carried out across the NRP, the degree of international collaboration, and the importance of European Commission funding to research projects. |
| Year(s) Of Engagement Activity | 2018 |
| Description | BBC Radio Norfolk Interview |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | I was interviewed on the potential effects of Brexit on research at The Sainsbury Laboratory, John Innes Centre and across the Norwich Research Park. The interview covered the type of research underway across the institutes, the international nature of research at TSL and JIC and the importance of European Commission funding to research programmes in Norwich. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Barley to Beer: Science into Practise |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Invited presentation at the Moleculr Biology of Plant Pathogens meeting, Norwich, Uk. The primary audience is PhD students |
| Year(s) Of Engagement Activity | 2019 |
| Description | Breeders Day 2018 |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Breeders Day is an annual event to present current research on crop improvement at the John Innes Centre. The 2018 event was focussed on the supply chain, emphasising downstream processing of crops into products |
| Year(s) Of Engagement Activity | 2018 |
| Description | Chair of Insectary Platform Steering Committee |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Chair of committee that ensures that the JIC Insectary/Entomology Technology Platform is well managed, proactive, effective, state of the art and resourced to meet the Institutes' science needs. |
| Year(s) Of Engagement Activity | 2017,2018,2019 |
| Description | Chair of Technical Platform Oversight Committee (TPOC) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Chair the committee that ensures that all JIC Technology Platforms are collectively well managed, proactive, effective, state of the art and resourced to meet the Institutes' science needs. |
| Year(s) Of Engagement Activity | 2017,2018,2019 |
| Description | Co-chair of Molecular Communications Workshop |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Conference co-organisor, Keynote Chair, Ideation Session Chair Molecular Communications, Linz, Austria Workshop aimed at promoting interdisciplinary research (computer scientists, telecommunications, engineers, biologists). Joint activities such as breakout groups to develop research projects and discuss concepts and ideas. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Co-organiser of the satellite meeting on Signal Transduction Pathways in Nitrogen-Fixing Symbioses |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The meeting focused on molecular, genetic and in vivo dynamic aspects of plant symbiotic signaling, and offered the possibility to highlight the recent progress in Nitrogen-fixing signal transduction. This satellite meeting provided a privileged frame for presentations, discussions and exchanges around the latest scientific discoveries in plant signal transduction related to Nitrogen-fixing symbiosis, that were not covered during the 13rd European Nitrogen Fixation Conference (ENFC) in Stockholm, Sweden. |
| Year(s) Of Engagement Activity | 2018 |
| URL | http://enfc2018.org/ |
| Description | Co-organization of the plant symbioses virtual seminar |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | The virtual seminar series occur every two months since the start of the COVID19 pandemic. The virtual seminar series aim at gathering the students, postdoctoral scientist, scientists and journal editors to stay connected and have an overview of the latest discoveries in Plant symbioses. |
| Year(s) Of Engagement Activity | 2020,2021 |
| Description | Conference attendance |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Brassica Research commuity is an informal meeting where researchers can present their latest results to academia and industry |
| Year(s) Of Engagement Activity | 2020 |
| Description | Crop Doctors - New Scientist Live |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | I presented alongside Prof Nick Talbot and Prof Mark Banfield in the New Scientist Live event representing and talking about research in the Plant Health ISP. Following presetations, I stayed online and answered many questions regarding GM technologies and plant disease. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Departmental seminar speaker - University of Geneva |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | I presented a research seminar and had meetings with University of Geneva faculty and students. I advised on novel experimental strategies for the Barberon group |
| Year(s) Of Engagement Activity | 2022 |
| Description | Disease resistance and immunity in brassicas: presentation to the Chinese Academy of Agricultural Sciences |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation of our research on brassica pathology to the Chinese Academy of Agricultural Sciences. The meeting took place at the John Innes Centre |
| Year(s) Of Engagement Activity | 2017 |
| Description | Disease resistance and immunity in brassicas: presentation to the Chinese Academy of Agricultural Sciences 2018 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Study participants or study members |
| Results and Impact | The annual meeting held with scientists from the Chinese Academy of Agricultural Sciences to present research updates and potential for collaborative research |
| Year(s) Of Engagement Activity | 2018 |
| Description | Disease resistance research on brassicas |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Industry/Business |
| Results and Impact | Presentations were given to CN Seeds and Clover seeds about research on brassica disease resistance. The work has led to further development of collaborative research with CN Seeds on downy mildew in brassicas for the baby leaf salad market. We also made a successful joint application for GCRF-funded research to develop collaboration between CN Seeds and a seed company in Nepal. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Electrical Signaling Invited Lecture (Max Planck) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Presentation on the underlying physics of signaling in plants. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Engagement with BBSRC as part of the BRIGIT project |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Have weekly meetings with policy makers at BBSRC about new policies that may be developed to reduce the risk of Xylella fastidiosa outbreaks in the UK |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | Engagement with Defra, Forest Research and APHA as part of the BRIGIT project |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Have weekly meetings with policy makers at Defra about new policies that may be developed to reduce the risk of Xylella fastidiosa outbreaks in the UK |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | Engagement with Fera as part of the BRIGIT project |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Have weekly meetings with Fera about status of Xylella fastidiosa diagnostics that may influence new policies for reducing risk of X. fastidiosa outbreaks in the UK |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | Engagement with Industry - Visit from Corteva and Indigro |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Engagement with Industry - Visit from Corteva and Indigro |
| Year(s) Of Engagement Activity | 2022 |
| Description | Engagement with Oxitec |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Regular meetings with Neil Morrison for supervisory meetings and discussing research progress of the iCASE studentship. |
| Year(s) Of Engagement Activity | 2015,2016,2017,2018,2019 |
| Description | Engagement with Syngenta |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Regular meetings with colleagues at Syngenta, Jealott's Hill, UK, and Switserland and USA to discuss project proposals and research progress on aphids. |
| Year(s) Of Engagement Activity | 2010,2011,2012,2013,2014,2015,2016,2017,2018,2019 |
| Description | Engagement with the Royal Horticulticultural Society as part of the BRIGIT project |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Third sector organisations |
| Results and Impact | Have weekly meetings with staff member of RHS to develop a stakeholder engagement plan for BRIGIT |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | European Calcium society webinar ; Invited speaker |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The talk was well received and open a debate on the differences and similarities between plants and animal calcium signalling. |
| Year(s) Of Engagement Activity | 2021 |
| Description | Exhibition |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Artwork created by Henry Driver and commissioned by Essex County Council to promote that food and the natural elements which sustain its' production, such as ample water & healthy soil should be cherished and regenerated, not wasted and degraded. |
| Year(s) Of Engagement Activity | 2020 |
| URL | http://www.henrydriverartist.com/regenerate.html |
| Description | Expert panel on molecular communications |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Expert panel to discuss the challenges and opportunities for molecular communications |
| Year(s) Of Engagement Activity | 2018 |
| Description | Fast-Track to Quantitative Disease Resistance in Brassicas |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | This was a presentation it industry as part of the Science for Innovation Showcase at John Innes Centre. This was followed up by formal meetings with industry representative about potential future collaborative research |
| Year(s) Of Engagement Activity | 2018 |
| Description | GCRF visit with Nepalese Researchers |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | A fact-finding mission to Nepal was undertaken with the aim of developing new collaborations for potential GCRF projects |
| Year(s) Of Engagement Activity | 2017 |
| Description | Genetic analysis of immune responses in brassicas |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation of research results at the 21st Crucifer Genetics Conference, St Malo, France. The audience was comprised of scientific researchers with a wide range of interests in crucifer genetics |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://colloque.inra.fr/brassica2018/ |
| Description | Growing the drinks industry in Wales |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Visitors from the Food and Drink Wales Cluster visited the John Innes Centre and we discussed the value of diverse germplam collections in developing new flavourse and sensory attributes of drinks |
| Year(s) Of Engagement Activity | 2019 |
| Description | Hosted Alexander Stacey, BSc student Biological Sciences, University of East Anglia, UK. |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Undergraduate students |
| Results and Impact | Student worked on a research project in the summer of 2018 and his BSc research project from Jan-Mar '19. |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | Immunity in brassicas |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Industry/Business |
| Results and Impact | Presentation to Elsoms, a seed and breeding company interested in collaborative research on oilseed rape improvement, especially in disease resistance |
| Year(s) Of Engagement Activity | 2018 |
| Description | Improving disease control in brassicaas |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Industry/Business |
| Results and Impact | Meeting with Prime agriculture to present research and explore potential collaborations in the future. Prime Agriculture are consultants in agriculture, with particular focus on agronomy. The work has led to an application to assess research priorities for disease control research in brassicas |
| Year(s) Of Engagement Activity | 2018 |
| Description | Interviewed by Symiah Barnett from POST to feed into an upcoming POST Note on the impacts and costs of synthetic fertilisers. |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Interviewed by Symiah Barnett from POST on 5 October to feed into an upcoming POST Note on the impacts and costs of synthetic fertilisers. Parliamentary briefing (POST note) - later on January 2024 - The future of fertiliser - was published. |
| Year(s) Of Engagement Activity | 2023 |
| Description | Invited Seminar at Bristol University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited seminar speaker at Bristol University - title 'The Plant Lytic Vacuole: Space-Filler, Garbage Bag, or Something More Interesting?' |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited Seminar at the Max Planck Institute for Plant Breeding Research (online) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I gave a seminar to the scientists and postgraduate students at the MPI, Cologne. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Invited Seminar at the Saclay Plant Sciences Network, Paris, France (online) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I gave a seminar to the scientists and postgraduate students at the Saclay Plant Scineces Network, France. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Invited Seminar for the CiSLT Network, Germany |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I gave a seminar to the scientists and postgraduate students within the CiSLT Network. These are researchers across Biology, Chemistry and Physics with an interest in chitin. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Invited Speaker - IOBC-WPRS PR-IR 2022: Priming the Future for Healthy Plants, Sheffield, UK |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Undergraduate students |
| Results and Impact | I was an invited speaker at IOBC-WPRS PR-IR 2022: Priming the Future for Healthy Plants, Sheffield, UK. This was attended by about 200 academic researchers and industry representatives. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Invited Speaker - Plant Methods Conference Czechia |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | I introduced our methods for assaying cell-to-cell communication in a seminar. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Invited Speaker: Host Microbe Interactions- Norwich Research Park Group |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Discussion within the Norwich research park scientists working on Host-Microbe Interactions in plant and animal. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited Talk - EMBO Workshop on Intercellular communication and plasmodesmata in development and disease |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Over 100 people attended the workshop to share progress in research and establish new collaborations |
| Year(s) Of Engagement Activity | 2022 |
| Description | Invited Talk - ETH, Zurich, Switzerland |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I presented our work relating to plasmodesmal function during immunity and infection. I also spent time discussing research projects with postdocs and faculy members. Discussions with the Rodriguez-Villalon lab led to the development of new strategies for experiments in their project. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Invited Talk - Plant Vascular Biology, Asilomar, California, USA |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | My attendance at this conference lead to informal discussions about 2 projects in my lab that have since lead to collaborations |
| Year(s) Of Engagement Activity | 2019 |
| Description | Invited conference talk - Plant Vascular Meeting Berlin |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Over 150 researchers attended to hear about out latest research findings. Lots of follow-up discussion. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Invited plenary speaker: 13th European Nitrogen Fixation Conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Prolific discussions and interest in nitrogen fixation signaling, as well as in promoting equality and diversity in science |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited seminar at ENS de Lyon, France |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | I gave a seminar to the scientists and postgraduate students at the ENS de Lyon, France. I also spoke with number of researchers in 1-1 meetings to exchange information and identify potential collaborations and exchanges. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Invited seminar at the 3rd Adam Kondorosi Symposium: Frontiers in Beneficial Plant Microbe Interactions |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The objective of this Symposium was to create a scientific event that is at the forefront of fundamental research in beneficial plant-microbe interactions. The Symposium brought together about 150 participants in a rather informal atmosphere, facilitating exchanges. This promoted exciting discussion with researchers - in particular those at the early stage of their career. |
| Year(s) Of Engagement Activity | 2017 |
| URL | https://symposium.inra.fr/ak-ips2-2017_eng/ |
| Description | Invited speaker - 'When development meets stress' B-Debate, Barcelona, Spain |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I participated in, and presented our reserach, in this cross-field debate in which researchers from developmental and stress biology met to discuss trade-offs and other issues that effect both areas of biology. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited speaker - International Microscopy Congress 19, Sydney, Australia |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I presented our work and live-imaging technologies at this technology focussed congress. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited speaker - PDRA/PGR Symposium, University of Glasgow UK |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | I was an invited speaker at the University of Glasgow PDRA/postgraduate student research symposium. I presented my research and career path ot the audience. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited speaker - Plant Peptides and Receptor Meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | I presented a research talk on to a specialised audience |
| Year(s) Of Engagement Activity | 2022 |
| Description | Invited speaker - VISCEA Plant Biotic Stresses and Resistance Mechanisms III, Vienna, Austria |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I presented my research to, and engaged in discussions with, participants at this symposium. This lead to some fruitful discussions regarding the similarities between my system and those of other researchers (i.e. plasmodesmata v extracellular vesicles) |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited speaker and session chair - International Congress of Molecular Plant Microbe Interactions |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The International Congress of Molecular Plant Microbe Interactions serves to disseminate cutting edge research developments in the field and was attended . I communicated our work relating to receptor specialisation at plasmodesmata and several audience members expressed that this expanded their views and understanding of plant responses to chitin. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Invited speaker at York University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited speaker to York University: Plant Biology Seminar - The Plant Lytic Vacuole: Space-Filler, Garbage Bag, or Something More Interesting? |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited speaker at the GARNet workshop on Advances in Plant Imaging, Warwick |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | A lecture on Calcium imaging was given to a research group interested in Plant Imaging. The positive outcome was demonstarted by an increase number of scientists looking forward to collaborate or to gain further experience in this area. |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://garnetcommunity.org.uk/node/988 |
| Description | Invited speaker at the XIX International Botanical Congress, Shenzhen, China |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Undergraduate students |
| Results and Impact | Invited speaker at on Calcium transport and signaling. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited speaker at the XVIII International Plant Microbe Interaction conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | The presentation was well received and led to new collaborations. |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://www.ismpmi.org/Congress/2019/Pages/default.aspx |
| Description | Invited speaker: 18th International Workshop on Plant Membrane Biology - Glasgow, UK |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Our research outcome was well received, which sparked questions and discussion afterwards. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Invited speaker: Gordon Research Conference on Organellar Channels and Transporters |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited speaker: Gordon Research Conference on Organellar Channels and Transporters Gordon Research Conference on Organellar Channels and Transporters |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited speaker: Gordon Research Conference on Organellar Channels and Transporters, Mount Snow, USA |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | This dedicated conference provided a unique opportunity for animal and plant scientists working on different organelles to share data and ideas to help addressing current challenges of organelle biology, foster collaborations, and identify new ways to treat common and rare diseases based on better understanding of mechanisms of ion/metabolite transport in various organelles. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited speaker: International Plant Molecular Biology Conference, Montpellier, France |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | stimulated discussion promoting new collaboration. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited speaker: Max Planck Institute for Plant Breeding Research, Cologne, Germany |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Exciting discussion promoting new collaboration |
| Year(s) Of Engagement Activity | 2019 |
| Description | Invited speaker: Max Planck Institute of Molecular Plant Physiology, Golm, Germany |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Discussion with scientists to increase collaborative effort aiming at improving crop production |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited speaker: SEB Functional organisation of the nuclear periphery, Florence, Italy |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | This session included presentations on recent advances in knowledge and understanding molecular functions, dynamics and interactions of proteins of the nuclear envelope across kingdoms; topics will include the proteins of the linker of cytoskeleton and nucleoskeleton complex; the nuclear pore complex; the nucleoskeleton and lamina and the interactions of the nuclear envelope with the cytoskeleton. It included applications ranging from the role of nuclear envelope proteins in plant stress and disease responses to developmental disorders caused by laminopathies. The session generated very stimulated discussions. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited talk - University of Sheffield, UK |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | I presented our research and spent time with researchers and students in one to one meetings. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Issue of JIC Advances magazine (January) focussed on Plant Health (Intro. to UN International Year of Plant Health) |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Issue of JIC Advances magazine (January) focussed on Plant Health (Intro. to UN International Year of Plant Health) |
| Year(s) Of Engagement Activity | 2020 |
| Description | JIC50 public open day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Open Day at JIC celebrating 50 years on the Norwich site. Soil columns featuring the N sensors were displayed to the public. |
| Year(s) Of Engagement Activity | 2017 |
| URL | https://www.jic.ac.uk/news-and-events/blog-copy/2017/08/john-innes-celebrates-50-years-norwich-2017 |
| Description | Keynote Speaker - Dynamo Symposium, Copenhagen, Denmark |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Undergraduate students |
| Results and Impact | I was a keynote speaker at the Dynamo Symposium, Copenhagen, Denmark. This was attended by academics, postgraduate students, undergraduate students, and representatives of Danish funding councils. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Keynote Speaker - EPS Get2gether, PhD student symposium, Netherlands |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | I presented my research and career progression to postgraduate students in the EPG Graduate School. After my presentation I engaged in discussion with students and a number were very interested in my career path. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Keynote Speech: Plants and Human Health at the Wellcome Trust China-UK Life Science Summit |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Keynote Speech: Plants and Human Health at the Wellcome Trust China-UK Life Science Summit |
| Year(s) Of Engagement Activity | 2017 |
| Description | Main organiser - EMBO workshop on Intercellular Communication in Development and Disease, Berlin, Germany |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | As main organiser of this workshop I secured funding from EMBO and shaped the programme of this workshop. We invited speakers from broad geographical locations and topic areas. The workshop feedback was overwhelmingly positive with a number of participants describing how they had established new collaborations from attending this workshop. The workshop has been passed on to a new group of organisers to arrange for a repeat event in 2020. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Mechanistic Analysis of Quantitative Disease Resistance in Brassica by Associative Transcriptomics 2018 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Study participants or study members |
| Results and Impact | This was the final workshop of MAQBAT to discuss results and plans for publications |
| Year(s) Of Engagement Activity | 2018 |
| Description | Meeting with Dales Land Net company |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Industry/Business |
| Results and Impact | Visit by Neale Hall from Dales Land Net company, Discuss collaboration to integrate moisture and nitrate soil sensors. |
| Year(s) Of Engagement Activity | 2023 |
| Description | Meeting with Novozymes Industry representatives |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Discuss future collaborations with Novozymes representatives (led by Jeanne Kjaer). Skype call with contacts in USA and India. (11 March 2019) I presented my current research and we discussed opportunites for future collaboration. Meeting facilitated by Jon Clarke at JIC. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Member Scientific Resources Committee |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Member of a committee that decides on purchases of all types of scientific equipment and organization of JIC infrastructure. |
| Year(s) Of Engagement Activity | 2017,2018,2019 |
| Description | Member of the Synthetic Biology Leadership Council |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Member of the Synthetic Biology Leadership Council |
| Year(s) Of Engagement Activity | 2017 |
| Description | Member of the UK Science Partnership for Animal and Plant Health |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Member of the UK Science Partnership for Animal and Plant Health - quarterly meetings are held |
| Year(s) Of Engagement Activity | 2017 |
| Description | Member of the interviewing panel for Herchel Smith Professorship of Biochemistry |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Member of the interviewing panel for Herchel Smith Professorship of Biochemistry position - University of Cambridge |
| Year(s) Of Engagement Activity | 2017 |
| Description | Membership of Scientific Advisory Committee to the Plant Energy Biology ARC Centre of Excellence |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Membership of Scientific Advisory Committee to the Plant Energy Biology ARC Centre of Excellence. An Australian National Centre. |
| Year(s) Of Engagement Activity | 2017 |
| Description | New Phytologist Next Generation Scientists meeting - Open Address |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Opening Address at New Phytologist Next Generation Scientists meeting - Scientific meeting |
| Year(s) Of Engagement Activity | 2017 |
| Description | New scientist live food and agriculture event The crop doctors. |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | New scientist live food and agriculture event The crop doctors. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Nourishing ten billion sustainably: resilient food production in a time of climate change |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Professional Practitioners |
| Results and Impact | Talk to retired medical doctors. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Nourishing ten billion sustainably: resilient food production in a time of climate change |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Policymakers/politicians |
| Results and Impact | CLIMATE CHANGE : SCIENCE AND SOLUTIONS | BRIEFING 10 The global food system accounts for around one third of greenhouse gas (GHG) emissions generated by human activity. It therefore offers a major opportunity for progress towards net zero if emissions can be reduced at the same time as delivering food security and building resilience to the inevitable impacts of climate change. Research shows how solutions can be found in diet change, respectfully approached, sustainable agricultural practices and harnessing the continuing wave of innovation in food biotechnology |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://royalsociety.org/-/media/policy/projects/climate-change-science-solutions/climate-science-so... |
| Description | Novel Approaches to Durable Disease Resistance in Crop Improvement |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Postgraduate students |
| Results and Impact | I gave an lecture at Imperial College for the module on Symbiosis, Immunity and Breeding to undergraduate and postgraduate students |
| Year(s) Of Engagement Activity | 2021 |
| Description | Novel Approaches to Durable Disease Resistance in Crop Improvement |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Plenary lecture at the Eurobiotech 2019 conference, Krakow, Poland |
| Year(s) Of Engagement Activity | 2019 |
| Description | Opening address at Breeders Day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Opening address at Breeders Day at the John Innes Centre |
| Year(s) Of Engagement Activity | 2017 |
| Description | Opportunities for AI in plant biology and crop research |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Plenary lecture to a wide audience including industry and academics on the potential for AI in plant research. There was a huge interest in our work, many questions, and follow up emails. |
| Year(s) Of Engagement Activity | 2021 |
| Description | Organised session of accessible science talks for schools |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Leading scientists from JIC and TSL presented their work in a accessible manner for support staff and pupils from 6 regional schools. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Organiser - Botanical Microscopy Conference |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | I was an organiser of the Botanical Microscopy Conference, hosted in Norwich. I also gave a research talk. This event involved a tour of JIC Bioimaging for participants and facilitated international exchange of knowledge regarding scientific advances and new methods. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Organization of the International Plant calcium signalling conference 2017 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | This conference brought all the plant calcium community together. It triggered very stimulating and interesting discussions and new opportunities of collaboration for many attendees. |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://plantcalciumsignalling.com/ |
| Description | PAMP responses in the Triangle of U |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | presentation of results from the MAQBAT project to update the brassica community about progress with the work. The presentation has enable further discussions about potential future collaborations and project ideas |
| Year(s) Of Engagement Activity | 2018 |
| Description | Panel Meeting of Severo Ochoa and Maria de Maeztu Committee - Madrid, Spain |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Panel Meeting of Severo Ochoa and Maria de Maeztu Committee - Madrid, Spain |
| Year(s) Of Engagement Activity | 2017 |
| Description | Panel discussion for the Royal Society for Hay Festival |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Hay Festival - panel discussion for the Royal Society exploring the public attitudes to the range of applications that genetic technologies might have, and the differences in such attitudes depending on context. This might look at the difference in public opinion in areas like food and medicine, for example, or explore concepts like 'naturalness' |
| Year(s) Of Engagement Activity | 2017 |
| Description | Plant Calcium Signalling Meeting CHANNELS THAT REGULATE CALCIUM MOVEMENT |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Free Calcium: A Paradigm for Ca2+ induced Ca2+ Release in Plants? Presentation at Plant Calcium Signalling Meeting CHANNELS THAT REGULATE CALCIUM MOVEMENT |
| Year(s) Of Engagement Activity | 2017 |
| Description | Plant calcium signalling conference; Invited speakers |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Invited speaker at the international Plant calcium signalling conference organized via zoom due to covid19. The talk was well received and several student, post-doc and group leaders debated with me afterward. |
| Year(s) Of Engagement Activity | 2021 |
| Description | Presentation at Chinese University Lecturers Conference - Fujian Agricultural University |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Miller A. J.(2018) Nitrogen uptake and assimialtion in Crop Plants (2 lectures) Fujian Agriculture and Forestry University, Fuzhou, China. Chinese University Lecturers Conference |
| Year(s) Of Engagement Activity | 2019 |
| Description | Presentation at Mardlers dinner |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Industry/Business |
| Results and Impact | The Mardlers are a group of approximately 25-30 Norfolk farmers, which all have reach beyond the farm gate, either through processing and marketing of their crops or stock, or through membership of industry bodies, political, research or marketing. There are also members who are involved with banking, accountancy and land agency, and as such they have a very broad range of interests across the agricultural, food and business sectors, both regionally and nationally. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Presentation at UK-China Workshop |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | 15-17 October Workshop at UEA. Chinese representatives from Anhui Agricultural University (AAU) and Jiangsu Academy of Agricultural Sciences (JAAS). Meeting organized by Dr Yuelai Lu (SAIN, School of International Development, UEA). Title of presentation: Soil Nitrogen and Crops |
| Year(s) Of Engagement Activity | 2018 |
| Description | Presentation to Suffolk Organic Gardners, Bury St Edmunds |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | 'Soils: root/microbial relationships and nutrient cycling' - presentation to the Suffolk Organic Gardeners Association in Bury St Edmunds (11 th February 2020). |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://www.gardenorganic.org.uk/suffolk-organic-gardeners |
| Description | Presentation to a group if Industry Representatives (BAYER) |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Presentation to BAYER - visiting group of representatives. The effects of a biostimulant on crops and developing soil nutrient sensors. 13 Feb 2019 at Church Farm. |
| Year(s) Of Engagement Activity | 2019 |
| URL | http://www.primeag.co.uk/ |
| Description | Presented talk at JIC "Science for Innovation Showcase" event |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Presented a talk at the JIC "Science for Innovation Showcase" event, Norwich, UK, 7-8 Feb '18. I explored opportunities to collaborate with industry. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Press release |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Press release to inform general public |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://www.jic.ac.uk/news/key-role-for-calcium-release-in-root-development/ |
| Description | Press release to announce new appointment for the BRIGIT project |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Engaged with JIC Public Engagement Officer to launch press release 'New appointment for UK-wide Xylella pathogen consortium' for the BRIGIT project |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://www.jic.ac.uk/news/new-appointment-for-uk-wide-xylella-pathogen-consortium/ |
| Description | PrimeAg visit |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Eight visitors from PrimeAg visited JIC for half a day. Prime Agriculture LLP is a consultancy partnership of 11 BASIS and FACTS qualified agronomists, working with growers in in Essex, Suffolk, Norfolk, Cambridgeshire, Bedfordshire, Northamptonshire and Hertfordshire. http://www.primeag.co.uk/ |
| Year(s) Of Engagement Activity | 2018 |
| URL | http://www.primeag.co.uk/ |
| Description | Roundtable debate on Genome Editing for Crop Improvement with Rt Hon Michael Gove MP, Secretary of State for the Environment. |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Took part in round table debate with the Secretary of State for the Environment to discuss the CJEU ruling on Genome Editing and how this was a potential impediment to innovation in crop improvement. The debate was co-ordinated by Tom Allen-Stevens and the NFU on 11th February 2018. The meeting solicited views from the science community, the soil association, the organic farming movement, Beyond GM, plant breeders, the AgBiotech industry, and the broader farming community. There is likely to be a follow-up discussion and ongoing work to advise government on genome editing and its potential use in crop improvement. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Royal Society fact finding visit to Beijing |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Royal Society fact finding visit to Beijing, 22 to 26 October for Royal Society's genetic technologies programme |
| Year(s) Of Engagement Activity | 2017 |
| Description | Science Breaker - How plants breathe |
| Form Of Engagement Activity | Engagement focused website, blog or social media channel |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | Working with engagement specialists, we developed an online 'Science Breaker' which explains the science to the general public and acts simultaneously as a forum for questions and answers. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Scientific Advisory Board Member for Max PIanck Institute of Molecular Plant Physiology, Golm, Germany |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Scientific Advisory Board Meeting for Max PIanck Institute of Molecular Plant Physiology |
| Year(s) Of Engagement Activity | 2017 |
| Description | Session chair - IS-MPMI Congress |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Undergraduate students |
| Results and Impact | I organised a session at the IS-MPMI congress in Providence, RI, USA. I also delivered a research talk. The session seeded an exchange of ideas that led to the proposal for a collaborative publication that represents advances in the field. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Soil and Plant conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presentation was titled: Soil health and nutrient supply to plants. Soil-Plant-Atmosphere Continuum 2015 Conference |
| Year(s) Of Engagement Activity | 2015 |
| Description | Talk - invited speaker "Mechanisms of Plant Perception - from the endogenous to the exogenous", University of Lausanne, Switzerland |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | I attended this symposium as an invited speaker. Discussion related to future possibile research directions and career development with postdocs and students. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Training in Downy Mildew propagation |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | We hosted two visitors from the Crop Research Institute, Czech Republic, to provide training in the maintenance of downy mildew cultures and their use in screening for disease resistance in Brassica oleracea |
| Year(s) Of Engagement Activity | 2019 |
| Description | Transatlantic Sessions in Conservation and Organic Agriculture |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | We held a workshop to discuss current thinking in conservation and organic agriculture, with speakers from UK and USA. This was an opportunity to discuss potential collaborations, and future joint research. These discussions are ongoing, though no specific projects have yet materialised |
| Year(s) Of Engagement Activity | 2018 |
| Description | Twitter campaign highlighting the UN International Year of Plant Health. 366 tweet-a-day throughout 2020. |
| Form Of Engagement Activity | Engagement focused website, blog or social media channel |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Twitter campaign highlighting the UN International Year of Plant Health. 366 tweet-a-day throughout 2020. |
| Year(s) Of Engagement Activity | 2020 |
| Description | University of Geneva - PhD Retreat |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Postgraduate students |
| Results and Impact | University of Geneva - PhD Retreat, participation in a debate: " Technological advances - A new chance for changing public perception on GMOs". Also speaker title: 'The plant lytic vacuole: space-filler, garbage bag, or something more interesting'. A two-day retreat for PhD students in molecular plant sciences at universities in French-speaking Switzerland. |
| Year(s) Of Engagement Activity | 2017 |
| Description | World Life Science Conference (Sustainable Agriculture Session), Beijing, 2018 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | China National Convention Center presentation: The Plant Vacuole: Roles in Plant and Human Nutrition, and Cellular Signaling 27th - 29th October 2018 |
| Year(s) Of Engagement Activity | 2018 |
| Description | presentsation to the Flying Farmers |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Flying farmers are a group of farmers interested to learn about the latest trends in research. the presentation involved talking to them about our work, and engaging in a discussion with them |
| Year(s) Of Engagement Activity | 2017 |