Chemical glycobiology
Lead Research Organisation:
John Innes Centre
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
The Field group is diverse in its activities, which range from organic synthesis to mechanistic enzymology and chemical biology. The common theme that runs throughout is carbohydrates. Current research themes include: the development of novel chemical and enzymatic approaches for the preparation of sugar nucleotides, oligosaccharides and glycosylated natural products; structural and mechanistic studies on enzymes involved in bacterial lipopolysaccharide, plant cell wall, plant and microbial and secondary metabolite biosynthesis; carbohydrate-coated surfaces (carbohydrate microarrays, glyco-nanoparticles and glyco-quantum dots) for identifying and quantifying protein-carbohydrate and pathogen-carbohydrate interactions; chemical genomics and chemical proteomics tools for plant cell and developmental biology with a particular emphasis on glycobiology (starch and plant cell metabolism, glycosylated secondary metabolites).
Planned Impact
unavailable
People |
ORCID iD |
| Rob Field (Principal Investigator) |
Publications
Mikhailova LR
(2009)
[A simple method of synthesis of triterpene glycosides similar to glycyrrhizic acid and their hepatoprotective activity in vitro].
in Bioorganicheskaia khimiia
Monestier M
(2017)
Membrane-enclosed multienzyme (MEME) synthesis of 2,7-anhydro-sialic acid derivatives
in Carbohydrate Research
Morton D
(2006)
Direct synthesis of chiral aziridines from N-tert-butyl-sulfinylketimines
in Chemical Communications
Mukhopadhyay B
(2007)
Corrigendum to "Glycosylation reactions with 'disarmed' thioglycoside donors promoted by N-iodosuccinimide and HClO4-silica"
in Tetrahedron Letters
Mukhopadhyay B
(2006)
Convergent synthesis of a trisaccharide as its 2-(trimethylsilyl)ethyl glycoside related to the flavonoid triglycoside from Gymnema sylvestre.
in Carbohydrate research
Mukhopadhyay B
(2009)
Bacterial detection using carbohydrate-functionalised CdS quantum dots: a model study exploiting E. coli recognition of mannosides
in Tetrahedron Letters
Mukhopadhyay Balaram
(2007)
From solution phase to "on-column" chemistry: Trichloroacetimidate-based glycosylation promoted by perchloric acid-silica. (vol 70, pg 9059, 2005)
in JOURNAL OF ORGANIC CHEMISTRY
Møller S
(2017)
Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD
in Scientific Reports
Møller SR
(2017)
Corrigendum: Identification and evolution of a plant cell wall specific glycoprotein glycosyl transferase, ExAD.
in Scientific reports
Ndeh D
(2017)
Complex pectin metabolism by gut bacteria reveals novel catalytic functions.
in Nature
| Description | New information on inhibitors of enzymes in starch metabolism, in model plant Arabidopsis and in barley. A new carbohydrate-binding virus that infect and lyse algae - a key factor in releasing fish-killing toxins from algal blooms. New enzymatic approaches for the preparation of oligosaccharides, based on glycoside phosphorylases and glycan sucrases Protein X-ray crystallography studies on enzymes involved in starch metabolism New carbohydrate-coated surfaces (glyco-nanoparticles) for identifying pathogen-carbohydrate interactions |
| Exploitation Route | Our virus/algal blooms work impacts on policy for waterways management and mitigation strategies. This is being followed up with the Environment Agency and the Broads Authority. Our enzymatic synthesis work has been taken forward with Innovate UK support - via the Glycoenzymes and GelEnz consortia. |
| Sectors | Agriculture Food and Drink Chemicals Environment Healthcare Manufacturing including Industrial Biotechology Pharmaceuticals and Medical Biotechnology |
| Description | Our work on glycosylated natural product toxins has had a major impact on algal bloom management on the Norfolk Broads, from work with the Environment Agency and the Broads Authority. Our enzymatic synthesis work has been taken forward with Innovate UK support - via the Glycoenzymes and GelEnz consortia. It has also been central to training ECRs from Thailand, with four returning to their homeland to take up academic positions (Tantanarat, Kuaudomlarp, Panpetch, Charoenwongpaiboon), all of whom continue to work in carbohydrate biotechnology. |
| First Year Of Impact | 2019 |
| Sector | Agriculture, Food and Drink,Environment,Pharmaceuticals and Medical Biotechnology |
| Impact Types | Societal Economic Policy & public services |
| Title | Enzymatic synthesis of HMO analogies |
| Description | Through detailed kinetic analysis, we have been able to understand how to use cellodextrin phosphorylase to transfer non-natural substrates to generate new to nature human milk oligosaccharide analogues |
| Type Of Material | Biological samples |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | Further studies ongoing to explore HMO analogue bioactivity. |
| Description | CDs |
| Organisation | Newcastle University |
| Country | United Kingdom |
| PI Contribution | Evaluation of a glycosynthase for novel cyclodextrin production |
| Collaborator Contribution | Provision of clone of wild type enzyme and active site mutant |
| Impact | Too early to judge |
| Start Year | 2017 |
| Description | Glycan synthesis |
| Organisation | Iceni Diagnostics |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Exchange of ideas and staff with regards the chemical synthesis of glycans and sugar phosphates. |
| Collaborator Contribution | Exchange of ideas and staff |
| Impact | Improved methods for the chemical synthesis of glycans and sugar phosphates. |
| Start Year | 2017 |
| Description | Glycoarrays |
| Organisation | Newcastle University |
| Country | United Kingdom |
| PI Contribution | Collaboration on glycoprofiling using microarray approaches to define the carbohydrate content of commercial prebiotics from Bangladesh. |
| Collaborator Contribution | Access to facilities and expertise to print carbohydrate microarrays. |
| Impact | Information for inclusion in publication in due course. |
| Start Year | 2017 |
| Description | MD starch |
| Organisation | University of Manchester |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Experimental evaluation of glycan structure and enzyme sensitivityt |
| Collaborator Contribution | Microsecond molecular dynamics simulation to assess glycan-glycan interactions |
| Impact | Too early |
| Start Year | 2017 |
| Description | PPase |
| Organisation | University of York |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Work up of scalable synthesis of N-glycan analogues |
| Collaborator Contribution | Clone of N-glycan phosphorylase |
| Impact | Too early |
| Start Year | 2017 |
| Company Name | Iceni Glycoscience |
| Description | Iceni Glycoscience develops carbohydrate-based therapeutics and point-of-care diagnostics for infectious diseases using their own research in glycoscience, nanoparticle technology, and bioconjugation chemistry. |
| Year Established | 2014 |
| Impact | Too early |
| Website | http://www.icenidiagnostics.com |
| Description | Algae on the Norfolk Broads |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Third sector organisations |
| Results and Impact | A series of stakeholder engagement and Pint of Science events were held to communicate to the public and relevant GROs and NGOs |
| Year(s) Of Engagement Activity | 2015,2016,2017,2018,2020 |