Impact of Hedgehog signalling on the T cell receptor (TCR) repertoire
Lead Research Organisation:
UNIVERSITY COLLEGE LONDON
Department Name: Cell and Developmental Biology
Abstract
We have shown that the Hedgehog signalling pathway regulates T-cell development in the thymus1,2. Sonic hedgehog (Shh) from thymic epithelial cells (TEC) signals to developing thymocytes to regulate their differentiation at key pre-T-cell receptor (pre-TCR) and TCR-dependent check-points1,2. Analysis of TCR-transgenic mice showed that Hh signalling reduced TCR signal strength during pre-TCR and TCR-induced differentiation2,3. We now plan to test the impact of these changes in TCR-signal strength induced by Hedgehog signalling on TCR repertoire selection, by next generation sequencing the TCR and TCR-chain repertoires from facs-sorted thymocyte populations at different stages of their development.
Organisations
People |
ORCID iD |
Publications
Lau CI
(2021)
The pioneer transcription factors Foxa1 and Foxa2 regulate alternative RNA splicing during thymocyte positive selection.
in Development (Cambridge, England)
Mengrelis K
(2019)
Sonic Hedgehog Is a Determinant of ?d T-Cell Differentiation in the Thymus.
in Frontiers in immunology
Solanki A
(2020)
The transcriptional repressor Bcl6 promotes pre-TCR-induced thymocyte differentiation and attenuates Notch1 activation.
in Development (Cambridge, England)
Yánez DC
(2021)
Systemic Pharmacological Smoothened Inhibition Reduces Lung T-Cell Infiltration and Ameliorates Th2 Inflammation in a Mouse Model of Allergic Airway Disease.
in Frontiers in immunology
Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| BB/M009513/1 | 30/09/2015 | 31/03/2024 | |||
| 1903458 | Studentship | BB/M009513/1 | 30/09/2017 | 02/03/2022 |