High-field NMR Spectroscopy for Biomolecular Research
Lead Research Organisation:
University of Bristol
Department Name: Chemistry
Abstract
NMR spectroscopy is the most information-rich analytical technique available to molecular research, but requires a high level of instrumentation to maximise the benefits of such analyses. In particular, its' application to materials of biological interest requires high quality instrumentation, with powerful superconducting magnets for signal resolution (500MHz or greater) and maximum sensitivity for proton nuclei. The Bristol NMR Facility currently supports a substantial number of researchers in the Biomolecular field and current NMR hardware is no longer sufficient to fulfil the demands of the cutting edge research projects conducted - which covers areas such as biosynthesis, protein structure and dynamics, potential disease treatments (Alzheimers and Cystic Fybrosis), bionanotechnology and membrane protein-ligand interactions. To this end, a modern 500MHz automated NMR spectrometer is required enabling these research fields to take advantage of the latest hardware and experimental developments required to achieve their aims.
Technical Summary
Biomolecular research at Bristol has a high demand for high resolution, high-sensitivity 1H-based NMR spectroscopy which cannot be fulfilled with current hardware. Over 60 biomolecular researchers require routine access to >400MHz spectroscopy in a range of projects spanning biosynthesis, protein structure and dynamics, bionanotechnology, membrane protein-ligand interactions, pharmaceutical development and ion transport in cell membranes. A 1H-sensitive, triple resonance 500MHz automated spectrometer will allow these research workers routine access to modern experimental methods (HSQC, field-gradient NOE, etc) at sufficiently high resolution for their needs. The rapid access to molecular structure elucidation, dynamics measurements and binding studies will allow more efficient and effective research programmes to be progressed and new projects to be developed at Bristol.
Publications
Adam C
(2018)
The Role of Terminal Functionality in the Membrane and Antibacterial Activity of Peptaibol-Mimetic Aib Foldamers.
in Chemistry (Weinheim an der Bergstrasse, Germany)
Dias CM
(2018)
Anthracene Bisureas as Powerful and Accessible Anion Carriers.
in Chemistry (Weinheim an der Bergstrasse, Germany)
Tassoti S
(2018)
High Resolution for Chemical Shifts and Scalar Coupling Constants: The 2D Real-Time J-Upscaled PSYCHE-DIAG.
in Chemphyschem : a European journal of chemical physics and physical chemistry
Hazelden IR
(2018)
Pyrrolidines and Piperidines by Ligand-Enabled Aza-Heck Cyclizations and Cascades of N-(Pentafluorobenzoyloxy)carbamates.
in Angewandte Chemie (International ed. in English)
Niwetmarin W
(2018)
(-)-Cytisine: Access to a stereochemically defined and functionally flexible piperidine scaffold.
in Organic & biomolecular chemistry
De Mattos-Shipley KMJ
(2018)
The cycloaspeptides: uncovering a new model for methylated nonribosomal peptide biosynthesis.
in Chemical science
Costil R
(2018)
a-Methyl phenylglycines by asymmetric a-arylation of alanine and their effect on the conformational preference of helical Aib foldamers.
in Organic & biomolecular chemistry
Mas-Roselló J
(2018)
Enantioselectively functionalised phenytoin derivatives by auxiliary-directed N to C aryl migration in lithiated a-amino nitriles.
in Chemical communications (Cambridge, England)
Dalling AG
(2018)
Synthesis of Nitrogen Heterocycles via Directed Carbonylative C-C Bond Activation of Cyclopropanes.
in Chimia
Harper MJ
(2018)
Oxidative Addition, Transmetalation, and Reductive Elimination at a 2,2'-Bipyridyl-Ligated Gold Center.
in Journal of the American Chemical Society
Farndon JJ
(2018)
Stereospecific Alkene Aziridination Using a Bifunctional Amino-Reagent: An Aza-Prilezhaev Reaction.
in Journal of the American Chemical Society
Shu C
(2018)
Synthesis of Functionalized Cyclopropanes from Carboxylic Acids by a Radical Addition-Polar Cyclization Cascade.
in Angewandte Chemie (International ed. in English)
Wang GW
(2018)
Modular Access to Azepines by Directed Carbonylative C-C Bond Activation of Aminocyclopropanes.
in Journal of the American Chemical Society
Ganesh V
(2018)
Chiral Aniline Synthesis via Stereospecific C(sp3)-C(sp2) Coupling of Boronic Esters with Aryl Hydrazines.
in Organic letters
Leonard D
(2018)
Asymmetric a-arylation of amino acids
in Nature
Amer M
(2018)
Substituted Dihydroisoquinolinones by Iodide-Promoted Cyclocarbonylation of Aromatic a-Amino Acids
in Organic Letters
Rubial B
(2018)
Enantiospecific Synthesis of ortho -Substituted 1,1-Diarylalkanes by a 1,2-Metalate Rearrangement/ anti -S N 2' Elimination/Rearomatizing Allylic Suzuki-Miyaura Reaction Sequence
in Angewandte Chemie International Edition
Nofiani R
(2018)
Strobilurin biosynthesis in Basidiomycete fungi.
in Nature communications
Lister FGA
(2018)
Bis-pyrene probes of foldamer conformation in solution and in phospholipid bilayers.
in Chemical science
Wu J
(2019)
Catalyst-Free Deaminative Functionalizations of Primary Amines by Photoinduced Single-Electron Transfer.
in Angewandte Chemie (International ed. in English)
Shu C
(2019)
Photoredox-Catalyzed Cyclobutane Synthesis by a Deboronative Radical Addition-Polar Cyclization Cascade.
in Angewandte Chemie (International ed. in English)
Dalling AG
(2019)
Carbonylative C-C Bond Activation of Electron-Poor Cyclopropanes: Rhodium-Catalyzed (3+1+2) Cycloadditions of Cyclopropylamides.
in Angewandte Chemie (International ed. in English)
Szwalbe AJ
(2019)
Characterisation of the biosynthetic pathway to agnestins A and B reveals the reductive route to chrysophanol in fungi.
in Chemical science
Fawcett A
(2019)
Strain-Release-Driven Homologation of Boronic Esters: Application to the Modular Synthesis of Azetidines.
in Journal of the American Chemical Society