Photoelectron spectroscopy in a liquid microjet: unravelling the excited state dynamics of photoactive proteins
Lead Research Organisation:
UNIVERSITY COLLEGE LONDON
Department Name: Chemistry
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Publications
Beekmeyer R
(2017)
Unravelling the electronic structure and dynamics of an isolated molecular rotary motor in the gas-phase.
in Chemical science
Bochenkova A
(2017)
Mechanism of resonant electron emission from the deprotonated GFP chromophore and its biomimetics
in Chemical Science
Fielding HH
(2018)
Using time-resolved photoelectron spectroscopy to unravel the electronic relaxation dynamics of photoexcited molecules.
in Chemical Society reviews
Greenwood JB
(2014)
Resonantly Enhanced Multiphoton Ionization Spectrum of the Neutral Green Fluorescent Protein Chromophore.
in The journal of physical chemistry letters
Henley A
(2018)
Role of Photoisomerization on the Photodetachment of the Photoactive Yellow Protein Chromophore.
in The journal of physical chemistry. A
Henley A
(2019)
An experimental and computational study of the effect of aqueous solution on the multiphoton ionisation photoelectron spectrum of phenol.
in Faraday discussions
Henley A
(2017)
Electronic structure and dynamics of torsion-locked photoactive yellow protein chromophores.
in Physical chemistry chemical physics : PCCP
Kirkby O
(2017)
Non-radiative relaxation dynamics of pyrrole following excitation in the range 249.5-200 nm
in Chemical Physics Letters
McLaughlin C
(2017)
ortho and para chromophores of green fluorescent protein: controlling electron emission and internal conversion.
in Chemical science
Mooney C
(2015)
Controlling Radical Formation in the Photoactive Yellow Protein Chromophore
in Angewandte Chemie
Mooney CR
(2015)
Controlling radical formation in the photoactive yellow protein chromophore.
in Angewandte Chemie (International ed. in English)
Mooney CR
(2014)
Competition between photodetachment and autodetachment of the 2(1)pp* state of the green fluorescent protein chromophore anion.
in The Journal of chemical physics
Parkes M
(2019)
A photoelectron imaging and quantum chemistry study of the deprotonated cyan fluorescent protein chromophore anion
in Molecular Physics
Parkes MA
(2016)
Controlling electron emission from the photoactive yellow protein chromophore by substitution at the coumaric acid group.
in Physical chemistry chemical physics : PCCP
Parkes MA
(2018)
A photoelectron imaging and quantum chemistry study of the deprotonated indole anion.
in Physical chemistry chemical physics : PCCP
Patel AM
(2020)
Shining light on the electronic structure and relaxation dynamics of the isolated oxyluciferin anion.
in Physical chemistry chemical physics : PCCP
Riley JW
(2018)
Unravelling the Role of an Aqueous Environment on the Electronic Structure and Ionization of Phenol Using Photoelectron Spectroscopy.
in The journal of physical chemistry letters
Riley JW
(2019)
Design and characterization of a recirculating liquid-microjet photoelectron spectrometer for multiphoton ultraviolet photoelectron spectroscopy.
in The Review of scientific instruments
Tau O
(2022)
Liquid-microjet photoelectron spectroscopy of the green fluorescent protein chromophore.
in Nature communications
Tay J
(2017)
The Effect of Conjugation on the Competition between Internal Conversion and Electron Detachment: A Comparison between Green Fluorescent and Red Kaede Protein Chromophores.
in The journal of physical chemistry letters
Woodhouse JL
(2017)
Photoelectron spectroscopy of isolated luciferin and infraluciferin anions in vacuo: competing photodetachment, photofragmentation and internal conversion.
in Physical chemistry chemical physics : PCCP
Woodhouse JL
(2019)
Photoelectron Imaging and Quantum Chemistry Study of Phenolate, Difluorophenolate, and Dimethoxyphenolate Anions.
in The journal of physical chemistry. A
Woodhouse JL
(2021)
A photoelectron imaging study of the deprotonated GFP chromophore anion and RNA fluorescent tags.
in Physical chemistry chemical physics : PCCP
| Description | We have developed a new instrument for uv photoelectron spectroscopy and time-resolved photoelectron spectroscopy in liquid microjets. We have published our first paper using this instrument and have data for other chromophores that is currently being analysed. |
| Exploitation Route | Our instrument is versatile so can be used for probing the photochemistry of a wide range of materials. |
| Sectors | Chemicals Healthcare |
| Title | Retrieval of true ultraviolet photoelectron spectra of aqueous solutions |
| Description | Combination of Monte Carlo simulations and spectral retrieval to convert UV photoelectron spectra of liquids to true spectra that are no longer distorted by the effect of inelastic scattering. |
| Type Of Material | Data analysis technique |
| Year Produced | 2022 |
| Provided To Others? | Yes |
| Impact | It has led us to collaborate with Dr Iain Wilkinson in Berlin, Professor Ruth Signorell in Zurich, and to us further developing this software. |
| Description | Collaboration with Berlin |
| Organisation | Helmholtz Association of German Research Centres |
| Department | Helmholtz-Zentrum Berlin for Materials and Energy |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | As a result of our work trying to understand the effect of inelastic scattering of low energy electrons in ultraviolet liquid-jet photoelectron spectroscopy, that arose from this grant, we have been awarded funding from the Royal Society for an exchange of PhD students between our group and the group of Iain Wilkinson in Berlin who works, primarily, on X-ray and extreme ultraviolet liquid-jet photoelectron spectroscopy. |
| Collaborator Contribution | We co-wrote the grant application. |
| Impact | None yet. |
| Start Year | 2023 |
| Description | EPSRC fellowship panel discussion |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | To encourage early career EPSRC fellows |
| Year(s) Of Engagement Activity | 2017 |
| Description | Equality and diversity lunchtime discussion, Newcastle University |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | To encourage women who wish to have an academic career in chemistry |
| Year(s) Of Engagement Activity | 2017 |
| Description | London institute for advanced light technologies, International Day of Light public science lecture |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | The event brought art and science together, and there was a general discussion about light and form. |
| Year(s) Of Engagement Activity | 2022 |
| Description | RSC careers evening |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | RSC careers evening |
| Year(s) Of Engagement Activity | 2017 |
| Description | Schools lecture |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Demonstration lecture - Light, Molecules, Action! |
| Year(s) Of Engagement Activity | 2017 |
| Description | UCL Chemical Engineering Athena SWAN PhD-PDRA-academic progression workshop |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | 20-30 PhD students attended the workshop, which sparked discussions about academic careers |
| Year(s) Of Engagement Activity | 2019 |