Cereal seed composition and end use quality
Lead Research Organisation:
Rothamsted Research
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
Cereals are the major arable crops in the UK and provide food, feed and raw materials for industry. Different end uses require different composition in terms of the amounts and compositions of the major grain components: starch, protein and cell wall polysaccharides. This project therefore uses a range of approaches (including metabolomics, transcriptomics and transgenesis) to dissect the pathways and mechanisms that determine cereal grain composition and to exploit this information to tailor the composition for traditional and novel end use requirements.
Specific targets are:-
1. Manipulating the amounts and composition of wheat endosperm cell walls for health (dietary fibre), feed and processing (distilling, biofuels).
2. Understanding the mechanisms by which proteins determine grain mechanical and functional properties.
3. Manipulating the patterns of gene expression and pathways of protein trafficking and deposition to develop novel wheats with improved processing properties.
Specific targets are:-
1. Manipulating the amounts and composition of wheat endosperm cell walls for health (dietary fibre), feed and processing (distilling, biofuels).
2. Understanding the mechanisms by which proteins determine grain mechanical and functional properties.
3. Manipulating the patterns of gene expression and pathways of protein trafficking and deposition to develop novel wheats with improved processing properties.
Planned Impact
unavailable
Organisations
People |
ORCID iD |
| Peter Shewry (Principal Investigator) |
Publications
Ang S
(2010)
Structure and heterogeneity of gliadin: a hydrodynamic evaluation.
in European biophysics journal : EBJ
Baudo* M
(2009)
Transgenic Wheat, Barley and Oats
Bechtel D
(2009)
WHEAT: Chemistry and Technology
Berecz B
(2010)
Structural stability and surface activity of sunflower 2S albumins and nonspecific lipid transfer protein.
in Journal of agricultural and food chemistry
Brijs K
(2009)
WHEAT: Chemistry and Technology
Chen P
(2010)
Heterologous expression and dough mixing studies of a novel cysteine-rich avenin-like protein
in Cereal Research Communications
Chen P
(2008)
Cloning, expression and characterization of novel avenin-like genes in wheat and related species
in Journal of Cereal Science
Fang J
(2009)
Cloning and molecular characterization of a novel x-type glutenin gene Glu-D(t)1 from Aegilops tauschii.
in Molecular biology reports
Field J
(2008)
Introgression of transgenes into a commercial cultivar confirms differential effects of HMW subunits 1Ax1 and 1Dx5 on gluten properties
in Journal of Cereal Science
Freeman J
(2011)
Temporal and spatial control of transgene expression using a heat-inducible promoter in transgenic wheat.
in Plant biotechnology journal
Funatsuki W
(2010)
Dough properties of transgenic wheat transformed with Cys-mutated LMW glutenin genes
in Proceedings of 10th International Gluten Workshop
Garino C
(2010)
Isolation, cloning, and characterization of the 2S albumin: a new allergen from hazelnut.
in Molecular nutrition & food research
Gil-Humanes J
(2011)
Suppression of gliadins results in altered protein body morphology in wheat.
in Journal of experimental botany
Gil-Humanes J
(2008)
Silencing of ?-gliadins by RNA interference (RNAi) in bread wheat
in Journal of Cereal Science
Godfrey D
(2010)
Effects of crop nutrition on wheat grain composition and end use quality.
in Journal of agricultural and food chemistry
Haward SJ
(2010)
Direct real-time imaging of protein adsorption onto hydrophilic and hydrophobic surfaces.
in Biopolymers
Haward SJ
(2011)
Force spectroscopy of an elastic peptide: effect of D2O and temperature on persistence length.
in Microscopy research and technique
Holopainen UR
(2012)
Day-length effects on protein localisation affect water absorption in barley (Hordeum vulgare) grains.
in Journal of the science of food and agriculture
Hughes SA
(2008)
In vitro fermentation of oat and barley derived beta-glucans by human faecal microbiota.
in FEMS microbiology ecology
Jones H
(2009)
WHEAT: Chemistry and Technology
Konarev A
(2008)
Serine proteinase inhibitors in seeds of Cycas siamensis and other gymnosperms
in Phytochemistry
Konarev AV
(2011)
Characterization of a glutenin-specific serine proteinase of Sunn bug Eurygaster integricepts Put.
in Journal of agricultural and food chemistry
Kuktaite R
(2011)
Structure and morphology of wheat gluten films: from polymeric protein aggregates toward superstructure arrangements.
in Biomacromolecules
Lambourne J
(2010)
Characterisation of an s-type low molecular weight glutenin subunit of wheat and its proline and glutamine-rich repetitive domain
in Journal of Cereal Science
Le Gall G
(2011)
Profiling of intact wheat arabinoxylans by spectroscopy
in New Food
Leon E
(2010)
Pasting properties of transgenic lines of a commercial bread wheat expressing combinations of HMW glutenin subunit genes
in Journal of Cereal Science
León E
(2010)
Stacking HMW-GS transgenes in bread wheat: Combining subunit 1Dy10 gives improved mixing properties and dough functionality
in Journal of Cereal Science
León E
(2009)
Mixing properties and dough functionality of transgenic lines of a commercial wheat cultivar expressing the 1Ax1, 1Dx5 and 1Dy10 HMW glutenin subunit genes
in Journal of Cereal Science
Lovegrove A
(2009)
Establishing substantial equivalence: proteomics.
in Methods in molecular biology (Clifton, N.J.)
Moore K
(2012)
Localisation of iron in wheat grain using high resolution secondary ion mass spectrometry
in Journal of Cereal Science
Moore KL
(2010)
NanoSIMS analysis of arsenic and selenium in cereal grain.
in The New phytologist
Peter Shewry (Author)
(2012)
Exploiting genetic variation to increase the dietary fibre content of wheat grain and flour
Quraishi UM
(2011)
Combined meta-genomics analyses unravel candidate genes for the grain dietary fiber content in bread wheat (Triticum aestivum L.).
in Functional & integrative genomics
Rakszegi M
(2009)
Puroindoline genes and proteins in tetraploid and hexaploid species of Triticum
in Journal of Cereal Science
Rosén LA
(2011)
Postprandial glycemia, insulinemia, and satiety responses in healthy subjects after whole grain rye bread made from different rye varieties. 1.
in Journal of agricultural and food chemistry
Sancho AI
(2010)
Authentication of food allergen quality by physicochemical and immunological methods.
in Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology
Saumonneau A
(2011)
Expression of a new chimeric protein with a highly repeated sequence in tobacco cells.
in Plant cell reports
Semenov MA
(2011)
Modelling predicts that heat stress, not drought, will increase vulnerability of wheat in Europe.
in Scientific reports
Shewry P
(2010)
Energy Crops
Shewry P
(2012)
An integrated study of grain development of wheat (cv. Hereward)
in Journal of Cereal Science
Shewry P
(2012)
Breadmaking
Shewry P
(2008)
Technology of Functional Cereal Products
Shewry P
(2012)
Preface to special issue on grain development
in Journal of Cereal Science
Shewry P
(2012)
Improving the nutritional quality and health benefits of wheat Abstract
in Quality Assurance and Safety of Crops & Foods
Shewry P
(2012)
Developing new types of wheat with enhanced health benefits
in Trends in Food Science & Technology
Shewry P
(2012)
Exploiting genetic variation to improve wheat composition for the prevention of chronic diseases
in Food and Energy Security
Shewry P
(2009)
Storage product synthesis and accumulation in developing grains of wheat
in Journal of Cereal Science
| Description | Identified key genes encoding enzymes of arabinoxylan synthesis in wheat and demonstrated their functions and effects on AX structure and amount by transgenesis Identified beta-glucan synthase genes in wheat and demonstrated effects on glucan structure and size by transgenesis Identified genetic sources of high arabinoxylan fibre in wheat for exploitation in breeding |
| Exploitation Route | Projects have been developed with plant breeders to develop high and low fibre wheat for human health and biofuels/distilling, respectively |
| Sectors | Agriculture Food and Drink |