Initiation and maintenance of vector arthropod colonies, elucidation of vector-virus interactions and the inheritance of competence and refractoriness
Lead Research Organisation:
THE PIRBRIGHT INSTITUTE
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
Unique colonies of several species of arthropod vectors are already maintained, with facilities available to expand into new arthropod groups or species as appropriate scientific opportunities arise. The current Culicoides colonies are vectors of important notifiable viruses including bluetongue virus and African horse sickness virus. The tick colonies are vectors of African swine fever virus. These resources enable the continuous provision of large numbers of individual vectors of known physiological state, thereby facilitating year-round research on the infection, replication and transmission of the study viruses by the vectors, and all aspects of virus-vector interactions. Also, the production by selective breeding of daughter colonies of individual vector species, each with a defined rate of oral susceptibility and transmission, and the sequencing of the genomes of individuals from such colonies, will provide unrivalled tools to investigate the inheritance and the genetic basis of susceptibility and refractoriness. These unique resources provide outstanding opportunities to promote multidisciplinary investigations (molecular, entomological, virological, genetic, modelling) and collaborations (both within and beyond IAH), and also facilitate training of colleagues at a range of levels from student to post-doctoral fellow.
Planned Impact
unavailable
Organisations
People |
ORCID iD |
| Simon Carpenter (Principal Investigator) |
Publications
Carpenter S
(2009)
Culicoides and the emergence of bluetongue virus in northern Europe.
in Trends in microbiology
Carpenter S
(2009)
Communication of bluetongue mitigation techniques.
in The Veterinary record
Papadopoulos E
(2010)
Treatment of horses with cypermethrin against the biting flies Culicoides nubeculosus, Aedes aegypti and Culex quinquefasciatus.
in Veterinary parasitology
Baylis M
(2010)
Evaluation of housing as a means to protect cattle from Culicoides biting midges, the vectors of bluetongue virus.
in Medical and veterinary entomology
Carpenter S
(2011)
Culicoides and the spread of bluetongue: finding the needle in the haystack.
in The Veterinary record
Darpel K
(2011)
Saliva Proteins of Vector Culicoides Modify Structure and Infectivity of Bluetongue Virus Particles
in PLoS ONE
Carpenter S
(2011)
Temperature dependence of the extrinsic incubation period of orbiviruses in Culicoides biting midges.
in PloS one
Kaufmann C
(2011)
Evaluation of matrix-assisted laser desorption/ionization time of flight mass spectrometry for characterization of Culicoides nubeculosus biting midges.
in Medical and veterinary entomology
Venail R
(2011)
Laboratory and field-based tests of deltamethrin insecticides against adult Culicoides biting midges.
in Journal of medical entomology
Peeters LM
(2012)
Evaluation of IgE levels against Culicoides nubeculosus allergens in Belgian warmblood horses.
in Communications in agricultural and applied biological sciences
Van Der Meide NM
(2012)
Culicoides obsoletus extract relevant for diagnostics of insect bite hypersensitivity in horses.
in Veterinary immunology and immunopathology
Veronesi E
(2013)
Implicating Culicoides biting midges as vectors of Schmallenberg virus using semi-quantitative RT-PCR.
in PloS one
Peeters LM
(2013)
Evaluation of an IgE ELISA with Culicoides spp. extracts and recombinant salivary antigens for diagnosis of insect bite hypersensitivity in Warmblood horses.
in Veterinary journal (London, England : 1997)
Nayduch D
(2014)
Studying Culicoides vectors of BTV in the post-genomic era: resources, bottlenecks to progress and future directions.
in Virus research
Seblova V
(2015)
The Biting Midge Culicoides sonorensis (Diptera: Ceratopogonidae) Is Capable of Developing Late Stage Infections of Leishmania enriettii.
in PLoS neglected tropical diseases
Manley R
(2015)
Testing of UK Populations of Culex pipiens L. for Schmallenberg Virus Vector Competence and Their Colonization.
in PloS one
Baker T
(2015)
Can insecticide-treated netting provide protection for Equids from Culicoides biting midges in the United Kingdom?
in Parasites & vectors
Rádrová J
(2016)
Hyaluronidase Activity in Saliva of European Culicoides (Diptera: Ceratopogonidae).
in Journal of medical entomology
Darpel KE
(2016)
Using shared needles for subcutaneous inoculation can transmit bluetongue virus mechanically between ruminant hosts.
in Scientific reports
| Description | The grant has enabled a wide range of studies to be conducted on insecticidal efficacy of products for Culicoides biting midges. In addition, the replication of arboviruses such as BTV has been characterized and translated to policy tools. Culicoides have also been used in experimental studies to infect animals with a variety of pathogens as part of vaccine trials and studies of pathogenesis. |
| Exploitation Route | Findings are being integrated into a wide range of outputs by both internal and external users (see publications). |
| Sectors | Agriculture Food and Drink Environment |
| Description | This is a service grant to supply arthropods (primarily Culicoides biting midges) to workers within the Pirbright Institute and also to external partners for a variety of projects. During the course of the project several hundred thousand midges were supplied of two species: C. sonorensis and C. nubeculosus. The former were used in areas as diverse as virus infection studies and saliva collection while the latter were largely used for insecticide testing. |
| Sector | Agriculture, Food and Drink,Environment |
| Impact Types | Economic |
| Description | European Biosecurity Talk, Ghent. Arthropod containment 15th May 2014. |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Formal presentation about arthropod security measures. Follow up with advice given to other institutes and HSE. |
| Year(s) Of Engagement Activity | 2014 |
| Description | International Congress of Entomology (Florida) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Chaired session and gave presentation on progress in Culicoides research. Largest ever meeting involving the Culicoides community with 100+ attendees. |
| Year(s) Of Engagement Activity | 2016 |
| URL | http://ice2016orlando.org/ |