Regulation of nutrient exchange in the Rhizobium-legume symbiosis
Lead Research Organisation:
John Innes Centre
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
Nitrogen fixation by the legume-Rhizobium symbiosis is driven by a supply of carbon from the plant, so by understanding this process we can actually determine which factors regulate the total amount of nitrogen fixed. The regulation of nutrient exchange between a legume and Rhizobium is largely determined by two membrane systems, one is plant derived and the other is bacterial. Our approach to understanding the interactions within and between these membranes is to mutate and clone the bacterial transport systems important for nutrient exchange. The genetics governing regulation of expression of these transport systems is being investigated and their effects on nitrogen fixation assessed.
Planned Impact
unavailable
Organisations
People |
ORCID iD |
| Philip Poole (Principal Investigator) |
Publications
Oldroyd GE
(2011)
The rules of engagement in the legume-rhizobial symbiosis.
in Annual review of genetics
Zhang Y
(2012)
NAD(P)+-malic enzyme mutants of Sinorhizobium sp. strain NGR234, but not Azorhizobium caulinodans ORS571, maintain symbiotic N2 fixation capabilities.
in Applied and environmental microbiology
Terpolilli JJ
(2012)
What determines the efficiency of N(2)-fixing Rhizobium-legume symbioses?
in Advances in microbial physiology
Karunakaran R
(2013)
Malonate catabolism does not drive N2 fixation in legume nodules.
in Applied and environmental microbiology
Udvardi M
(2013)
Transport and metabolism in legume-rhizobia symbioses.
in Annual review of plant biology
Poole P
(2017)
Shining a light on the dark world of plant root-microbe interactions.
in Proceedings of the National Academy of Sciences of the United States of America
| Description | Botanic Gardens seminar |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | I talked to the Botanic Gardens about nitrogen and humanity Lots of questions and feedback. This is widely advertised to the public |
| Year(s) Of Engagement Activity | 2014 |
| Description | Give peas a chance |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | We made a film with Oxford sparks on nitrogen fixation Lots os hits on the web site |
| Year(s) Of Engagement Activity | 2014 |
| Description | Meeting minds Oxford Alumni |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | I gave a talk to the Oxford biological sciences Alumni Enthusiastic response from the audience |
| Year(s) Of Engagement Activity | 2014 |
| Description | Schools one week Intensive course |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | Intensive one week course for A level students in practical plant and microbial science Students all keen to apply for science based university courses |
| Year(s) Of Engagement Activity | 2014 |
| Description | Wymondham academy school visit |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Talk to 6th form students to encourage them to consider science as a career Students keen to apply for university places |
| Year(s) Of Engagement Activity | 2014 |