Light entrainment of the circadian clock: identifying natural molecular adaptations

Lead Research Organisation: University of Leicester
Department Name: Genetics

Abstract

Circadian clocks are molecular pacemakers that drive daily rhythms in physiology, metabolism, behaviour and other process, and are present in diverse range of organism, from cyanobacteria to human. When detached from ambient cues, circadian clock cycle ('freerun') at periods slightly different from 24 hr. This endogenous rhythm is adjusted to the 24 hr solar day by entrainment to various stimuli, primarily light. Modern life introduces situations, such as trans-atlantic flights and shift work, where the circadian rhythm and the external light cycle are too dissimilar to reconcile; accumulating evidence suggests that people exposed repeatedly to such disruptions suffer from wide range of health problems, including 'jetlag', sleep disorders, seasonal depression, and cancer. In the last few decades, a great deal has been learned about the molecular details of the clock. Drosophila has been instrumental in identifying circadian clock genes, which are well conserved in mammals, both in sequence and function. By inducing mutagenesis and screening for Drosophila mutants that show aberrant light response, two proteins were identified to be involved in light transduction: TIMELESS (TIM), which is a circadian light-sensitive core-clock protein, and CRYPTOCHROME (CRY), a dedicated blue-light photoreceptor of the circadian system. These two proteins interact with each other, and light-activated CRY attaches itself to TIM, degrading it rapidly. Our research at the University of Leicester focuses on natural genetic variation related to circadian photo-responsiveness. Rather than inducing random mutations, we aim to understand the clock mechanism by identifying natural variants, or natural clock alleles that have been evolved in different wild populations, serving as molecular adaptations under different light and temperature conditions. In collaboration with CPK and ER at Leicester and the Costa lab at Padova, we have identified a natural polymorphism in Timeless that involves a single-base insertion/deletion, situated between two alternative translation starts. We have found that this polymorphism follows a robust latitudinal cline and is maintained by directional selection. We subsequently tested natural isolates and transformants flies and found that photo-responsiveness is significantly different between flies with the different alleles. This difference was correlated with the variation we have observed in the photoperiodic response of flies with the different alleles suggesting that this polymorphism represents molecular adaptation to cold environments. Recently, another protein named JETLAG (JET) has been identified as being involved in light-induced degradation of TIM. Interestingly, it turns out that the phenotype of jet mutants is only expressed in strains carrying a specific natural tim allele. These discoveries have demonstrated how natural genetic variation modulates light sensitivity of the circadian clock, and how in turn, could the better characterization of natural adaptations lead to a better understanding of the circadian-clock mechanism. The current proposal is aimed at identifying natural variation in clock genes by testing strains derived from wild-populations, using tools of quantitative genetics combined with molecular techniques. We propose to use various genome-wide screens to identify these variations, including Quantitative Trait Loci (QTL) mapping, artificial selection and global expression analysis. Our preliminary QTL screen indicated four genomic regions (QTLs) that show significant contribution to variation in circadian light-sensitivity. By using various deficiency and mutant strains we will carry complementation tests that will allow us to identify the causal sequence variations that account for these variations. The results of this study will allow a better understanding of light entrainment of the clock, and provide candidate genes for studying in mammals, including human.

Technical Summary

The current proposal is aimed to identify natural genetic variations that affect circadian light sensitivity. These variations have been driven by natural selection, serving as molecular adaptations under various light and temperature conditions. In the lab, we use light-pulse experiments to measure circadian light sensitivity: brief light stimulus is presented to animals maintained in continuous darkness. Most organisms, including Drosophila, will respond by shifting their free-running rhythm to a new phase. The size of phase shift corresponds to the light-sensitivity of the fly. We have used quantitative trait loci (QTL) mapping and identified four significant QTLs that contribute to variation in light sensitivity. To narrow down these QTL intervals, we will carry complementation tests using a series of overlapping deficiency fly strains. Crossing these deficiency strains to the QTL parental lines and testing their light response will allow us to further reduce these QTL intervals. After reducing the QTLs to several kb long, fragments will be cloned and sequenced and nucleotide variations between the QTL parental lines will be identified. Candidate alleles for different variations will be transformed into flies by homologous recombination. Comparing the light sensitivity of these transgenic flies that express different natural alleles will indicate which polymorphic sites are important for circadian light sensitivity. We will also use artificial selection to produce lines that differ in their light sensitivity. The response to selection will provide information about the genetic architecture of this trait, and the selected lines will be tested for divergent gene expression. Differentially expressed genes will be sequenced and polymorphic sites will be identified and tested as above. Finally, we will also exploit variation in light sensitivity between two Drosophila species using custom microarrays and transformant flies.
 
Description The project was aimed at identifying natural polymorphisms in genes that modulate circadian light sensitivity and serve as molecular adaptations in various light/temperature conditions.
We assayed the circadian photosensitivity of 123 recombinant inbred (RI) strains generated at the lab of Sergey Nuzhdin at the University of Southern California. Extensive variation in the light response of these lines indicated a substantial heritability of 30% . Interval mapping has indicated a single significant QTL on chromosome 2. We used quantitative deficiency complementation tests, using fly strain carrying defined chromosomal deletions, and refined this QTL interval into two smaller QTL consisting of 140 candidate genes. Complementation tests to null mutation strains suggested segregating alleles of timeless (tim) (and possible cycle ) contribute to the variation in light response. Further genotyping revealed that the previously reported (European) s/ls polymorphism in tim is segregating in our RI strain collection (derived from North-America population), and as expected, is associated with variation in light response of RI strains.
To identify candidate genes within these QTLs we carried a global gene expression profiling by microarrays. Two recombinant inbred lines that show different photo-sensitivity, 104 (High) and 58 (Low) were used. RNA was extracted from heads of treated (light-pulse at Zt15) and the control flies (no-pulse), and labelled cDNA was generated and hybridised (a two colour design) to Drosophila oligonucleotide microarrays. We identified the top 500 genes that show large fold change in strain 104 (H), compared to 58 (L). Six genes are located in the QTL interval and may serve as candidates for causative genes underlying the light-sensitivity QTL. A manuscript summarising the QTL mapping is in preparation.
In another set of experiments we profiled gene expression in a laboratory wild-type strain (Canton-S) using Affymetrix GeneChips. To date, this is the first dataset in Drosophila showing the whole transcriptome response to light pulse. The analysis revealed 209 differentially expressed genes, with several biological processes such as signal transduction, gene regulation, glutamate receptor activity, cellular communication and chromatin remodelling were statistically over-represented. Targeted knockdown of these genes by dsRNAi in clock neurons has further demonstrated the role of these genes in the light response. In addition, the microarray experiments indicated that histone modifications may also play an important role in light entrainment of the clock. Consistently, an aberrant light response was associated with 6 genes, using mutant and transgenic strains in which histone acetylation, de-acetylation, and methylation (of DNA and histones) is defective.
Exploitation Route Our study has generated a large number of candidate genes that play important role in light entrainment of the circadian clock. This provide ideal starting point for other research team to explore the mechanism by which these gene (or genetic variation which these gene) regulate circadian sensitivity. Given the high level of conservation of the circadian system in flies and in human, these candidate genes are likely to be useful for diagnostic of health issues relate to light entrainment of the clock in human, including seasonal depression and shift-word associated sleep disorders.
Sectors Agriculture, Food and Drink,Healthcare,Pharmaceuticals and Medical Biotechnology

 
Description Genetic dissection of seasonal timing in Drosophila
Amount £483,457 (GBP)
Funding ID BB/K001922/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2013 
End 01/2016
 
Description H2020-MSCA-ITN-2017
Amount € 3,809,005 (EUR)
Funding ID 765937 
Organisation European Commission H2020 
Sector Public
Country Belgium
Start 05/2018 
End 05/2021
 
Description Individual Research Grant
Amount 1,088,100 ש"ח (ILS)
Funding ID 1737/17 
Organisation Israeli Science Foundation 
Sector Charity/Non Profit
Country Israel
Start 10/2017 
End 10/2021
 
Description Light entrainment of the circadian clock: identifying natural molecular adaptations
Amount £293,672 (GBP)
Funding ID BB/G02085X/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 02/2010 
End 01/2013
 
Description Molecular and Cellular Dissection of Morning and Evening Chronotypes in Drosophila melanogaster
Amount £629 (GBP)
Funding ID BB/H018093/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 02/2011 
End 01/2014
 
Description The genetics and genomics of adaptive sex ratio behaviour
Amount £588,581 (GBP)
Funding ID NE/J024481/1 
Organisation Natural Environment Research Council 
Sector Public
Country United Kingdom
Start 04/2012 
End 03/2015
 
Description Timeless and diapause in Drosophila
Amount £491 (GBP)
Funding ID BB/F014082/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2008 
End 12/2012
 
Title Differential expression of microRNA associated with photoperiod in Drosophila 
Description global expression of microRNA 
Type Of Material Database/Collection of data 
Year Produced 2011 
Provided To Others? Yes  
Impact This is the first demonstration of the role of microRNA in seasonal timing, and regulation of insect diapause. 
 
Title Expression analysis of circadian light response in Drosophila 
Description Expression data generated by by global profiling using microarrays. GEO -NCBI; Accession: PRJNA171155; GEO: GSE39578 
Type Of Material Database/Collection of data 
Year Produced 2012 
Provided To Others? Yes  
Impact No actual impacts realised to date 
URL http://www.ncbi.nlm.nih.gov/bioproject/PRJNA171155
 
Title Expression analysis of circadian light response in Drosophila recombinant inbred lines (RIL) 
Description Gene expression profiling by microarrays, comparing two Drosophila recombinant inbred lines, which differed significantly in their circadian light response. GEO accession: GSE77116 
Type Of Material Database/Collection of data 
Year Produced 2015 
Provided To Others? Yes  
Impact NA 
URL http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE77116
 
Title Photoperiodic induction of gene expression in Drosophila 
Description Microarray data 
Type Of Material Database/Collection of data 
Year Produced 2011 
Provided To Others? Yes  
Impact This database provides, for the first time, a list of candidate genes associated with seasonal timing and the photoperiodic response. 
 
Description BBSRC-funded Midlands Integrative Bioscience Training Partnership. 
Organisation University of Birmingham
Country United Kingdom 
Sector Academic/University 
PI Contribution A joint doctoral programme of the universities of Leicester, Birmingham, and Warwick.
Start Year 2012
 
Description BBSRC-funded Midlands Integrative Bioscience Training Partnership. 
Organisation University of Warwick
Country United Kingdom 
Sector Academic/University 
PI Contribution A joint doctoral programme of the universities of Leicester, Birmingham, and Warwick.
Start Year 2012
 
Description CINCHRON 
Organisation Academy of Sciences of the Czech Republic
Country Czech Republic 
Sector Academic/University 
PI Contribution Planning and preparation of the consortium research proposal for a Marie Curie ETN (CINCHRON). Supervision and training of PhD students in our laboratory.
Collaborator Contribution Planning and preparation of the consortium research proposal for a Marie Curie ETN (CINCHRON). Supervision and training of PhD students in their laboratory.
Impact MSCA-ITN-ETN award by the Eurpean Comission (proposal number 765937). 3,809,005 EUR.
Start Year 2016
 
Description CINCHRON 
Organisation Hebrew University of Jerusalem
Country Israel 
Sector Academic/University 
PI Contribution Planning and preparation of the consortium research proposal for a Marie Curie ETN (CINCHRON). Supervision and training of PhD students in our laboratory.
Collaborator Contribution Planning and preparation of the consortium research proposal for a Marie Curie ETN (CINCHRON). Supervision and training of PhD students in their laboratory.
Impact MSCA-ITN-ETN award by the Eurpean Comission (proposal number 765937). 3,809,005 EUR.
Start Year 2016
 
Description CINCHRON 
Organisation Jagiellonian University
Country Poland 
Sector Academic/University 
PI Contribution Planning and preparation of the consortium research proposal for a Marie Curie ETN (CINCHRON). Supervision and training of PhD students in our laboratory.
Collaborator Contribution Planning and preparation of the consortium research proposal for a Marie Curie ETN (CINCHRON). Supervision and training of PhD students in their laboratory.
Impact MSCA-ITN-ETN award by the Eurpean Comission (proposal number 765937). 3,809,005 EUR.
Start Year 2016
 
Description CINCHRON 
Organisation National Center for Scientific Research (Centre National de la Recherche Scientifique CNRS)
Country France 
Sector Academic/University 
PI Contribution Planning and preparation of the consortium research proposal for a Marie Curie ETN (CINCHRON). Supervision and training of PhD students in our laboratory.
Collaborator Contribution Planning and preparation of the consortium research proposal for a Marie Curie ETN (CINCHRON). Supervision and training of PhD students in their laboratory.
Impact MSCA-ITN-ETN award by the Eurpean Comission (proposal number 765937). 3,809,005 EUR.
Start Year 2016
 
Description CINCHRON 
Organisation University of Leicester
Department Department of Genetics
Country United Kingdom 
Sector Academic/University 
PI Contribution Planning and preparation of the consortium research proposal for a Marie Curie ETN (CINCHRON). Supervision and training of PhD students in our laboratory.
Collaborator Contribution Planning and preparation of the consortium research proposal for a Marie Curie ETN (CINCHRON). Supervision and training of PhD students in their laboratory.
Impact MSCA-ITN-ETN award by the Eurpean Comission (proposal number 765937). 3,809,005 EUR.
Start Year 2016
 
Description CINCHRON 
Organisation University of Padova
Country Italy 
Sector Academic/University 
PI Contribution Planning and preparation of the consortium research proposal for a Marie Curie ETN (CINCHRON). Supervision and training of PhD students in our laboratory.
Collaborator Contribution Planning and preparation of the consortium research proposal for a Marie Curie ETN (CINCHRON). Supervision and training of PhD students in their laboratory.
Impact MSCA-ITN-ETN award by the Eurpean Comission (proposal number 765937). 3,809,005 EUR.
Start Year 2016
 
Description CINCHRON 
Organisation University of Wurzburg
Country Germany 
Sector Academic/University 
PI Contribution Planning and preparation of the consortium research proposal for a Marie Curie ETN (CINCHRON). Supervision and training of PhD students in our laboratory.
Collaborator Contribution Planning and preparation of the consortium research proposal for a Marie Curie ETN (CINCHRON). Supervision and training of PhD students in their laboratory.
Impact MSCA-ITN-ETN award by the Eurpean Comission (proposal number 765937). 3,809,005 EUR.
Start Year 2016
 
Description NC3R joint application 
Organisation Medical Research Council (MRC)
Department MRC Human Genetics Unit
Country United Kingdom 
Sector Academic/University 
PI Contribution NC3R joint application
Start Year 2013
 
Description ABC Radio Interview (Melbourne, Australia) 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact Interview to ABC Radio , Melbourne, Australia, covering our research on the genetics of diurnal preference and circadian clocks.
Year(s) Of Engagement Activity 2015
 
Description Interview BBC Radio Leicester 2014 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact t Jonathan Lampon morning show. The interview sparked questions and discussions.

Impact unknown
Year(s) Of Engagement Activity 2014
URL http://www.bbc.co.uk/programmes/p025jkkb
 
Description 'Plasticity in Circadian Rhythms and Sleep' workshop 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact An invited talk at the 'Plasticity in Circadian Rhythms and Sleep' workshop at the Hebrew University orgenised by the Jerusalem Brain Community. The talk was entitled 'Epigenetic regulation of the photoperiodic clock' and sparked questions and discussion afterwards.
Year(s) Of Engagement Activity 2018
URL https://scholars.huji.ac.il/jbc/event/save-date-jbc-organized-workshop-plasticity-circadian-rhythms-...
 
Description Career talk at Beauchamp College open day (MP) 2014 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach Local
Primary Audience Schools
Results and Impact career talk I gave to the Beauchamp College open day. talk sparked questions and discussion afterwards.

Impacts are not known yet.
Year(s) Of Engagement Activity 2014
 
Description Chronobiolgy Biology Summer school (Bangalore) 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Lectures at the SERC Summer School in Chronobiology, Bangalore, India

After my talk, I was approached by several students that were interested in joining my research team.
Year(s) Of Engagement Activity 2009
 
Description Does the Drosophila seasonal timer require a circadian clock? SRBR 2014 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The presentation sparked questions and discussion afterwards

Impact has not been realised yet.
Year(s) Of Engagement Activity 2014
URL http://www.srbr.org/Pages/SRBR_Meeting.aspx
 
Description Dynamic DNA 2011 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Schools
Results and Impact Presentation and hands-on activities on Drosophila as model organism, created and delivered by the Tauber lab, as part of a two-day event organised by GENIE to year 9 students.

no actual impacts realised to date
Year(s) Of Engagement Activity 2011
 
Description Dynamic DNA 2012 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Presentation and hands-on activities delivered by the Tauber lab members on Drosophila as model system, as part of the event organised by GENIE to year 9 students. The activities sparked questions and discussion afterwards.

no actual impacts realised to date
Year(s) Of Engagement Activity 2012
URL http://www2.le.ac.uk/news/blog/2012/september/dynamic-dna-demonstration
 
Description Dynamic DNA 2013 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Hands-on activities for school children demonstrating the use of fruitflies in Genetics, as part of the Dynamic DNA day orgenised by GENIE, at the University of Leicester. The activities sparked questions and discussions afterwards.

no actual impacts realised to date
Year(s) Of Engagement Activity 2013
URL http://www2.le.ac.uk/departments/genetics/genie/outreach/dynamic-dna
 
Description Dynamic DNA 2014 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Lecture and hands-on activities with fruit-flies, demonstrating the importance of the concept of model organism in genetic research. Part of 'Dynamic DNA' two days orgenised by the GENIE CETL at the University of Leicester.

Teacher reported that the activities aid pupils' understanding of basic concepts in Genetics.
Year(s) Of Engagement Activity 2014
 
Description Dynamic DNA 2016 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact During two days 417 pupils from various schools have visited the department of genetics and carried out various hand-on activities on genetics. We have provided information and activities about the importance of the fruit-fly as model system for genetic research
Year(s) Of Engagement Activity 2016
 
Description How fruit flies teach us about Genetics and the Brain! 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Geographic Reach Regional
Primary Audience Schools
Results and Impact talk sparked questions and discussion afterwards.

no actual impacts realised to date
Year(s) Of Engagement Activity 2012
 
Description Interview for Heredity Podocasts (March 2012) 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Interview published as an Internet podcast by the Heredity journal. Podcast (March 2012 episode). Biological clocks - links between daily and seasonal rhythms.

no actual impacts realised to date
Year(s) Of Engagement Activity 2012
URL http://webspace.qmul.ac.uk/ranichols/podcast/
 
Description Interview to The Marker. Daily economic newspaper (Israel) 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact An article discussing our research entitled "Find it difficult to get up in the morning ? The reason lies in the fruit fly" (in Hebrew). http://www.themarker.com/news/1.2735883
Year(s) Of Engagement Activity 2015
URL http://www.themarker.com/news/1.2735883
 
Description Lecture at Oakham School 2012 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach local
Primary Audience Schools
Results and Impact Lecture to sixth form student at Oakham School entitled:"The fruitfly: a key model organism for understanding genetics and the brain".

no actual impacts realised to date
Year(s) Of Engagement Activity 2012
 
Description LiveGene! school visit at Beaumont Leys School 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Schools
Results and Impact LiveGene! is a project run by the Tauber lab promoting using live fruitflies in the classroom for genetic education. The team visited Beaumont Leys School in Leicester and run various experiments with year 10 students (age 14-15)

no actual impacts realised to date
Year(s) Of Engagement Activity 2014
 
Description LiveGene! training January 2014 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact LiveGene! is a project run by the Tauber Lab, promoting the use of live fruit flies in the classroom for genetic education. A training in handling flies was given to school teachers and technicians from 5 different schools in Leicestershire. The training was carried in the GENIE lab at the University of Leicester Kits and live strain of Drosophila for experiments in the classroom

Printed material with experiment and lesson plans.

After the training, teachers adopted the use of live fruitflies in the genetic lessons in the classroom.
Year(s) Of Engagement Activity 2014
URL http://www.tinyURL.com/liveGene
 
Description LiveGene! training July 2013 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach local
Primary Audience Schools
Results and Impact LiveGene! is a project run by the Tauber Lab, promoting the use of live fruit flies in the classroom for genetic education. A training in handling flies was given to school teachers and technicians from 7 different schools in Leicestershire. The training was carried at the GENIE lab at the University of Leicester Kits and live strains of Drosophila for experiments in the classroom.

Printed material with background and lesson plans.

no actual impacts realised to date
Year(s) Of Engagement Activity 2013
 
Description LiveGene! training June 2013 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact LiveGene! is a project run by the Tauber Lab, promoting the use of live fruit flies in the classroom for genetic education. A training in handling flies was given to school teachers from Ashby School. The traning was carried at the GENIE lab at the University of Leicester Kits and live strains of Drosophila for experiments in the classroom.

Printed material with experiment and lesson plans.

After the training, teachers have adopted the suggested activities with fruitflies in their school.
Year(s) Of Engagement Activity 2013
URL http://www.tinyURL.com/liveGene
 
Description LiveGene! training at Southfield School 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact LiveGene! is a project run by the Tauber Lab, promoting the use of live fruit flies in the classroom for genetic education. A training in handling flies was given to school teachers and technicians at Southfield School, Kettering.

no actual impacts realised to date
Year(s) Of Engagement Activity 2013
 
Description Modern genetic techniqes workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Full day of Lectures and practical sessions on molecular biology techniques (cloning, PCR) to six form students from Kettering Buccleuch Academy, run in GENIE lab at the Univeristy of Leicester, by the LiveGene! team.

After the session, the teacher said that students reported better understanding of the thought material in class and felt better prepared to GCSE.
Year(s) Of Engagement Activity 2014
 
Description National Science & Engineering Week 2014 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Geographic Reach local
Primary Audience Schools
Results and Impact A lecture at Beauchamp College (Leicester) during National Science & Engineering Week, explaining the concept of model oragnism and the contribution of Drosophila to research in genetics. Year 12 students (age 16-17).

no actual impacts realised to date
Year(s) Of Engagement Activity 2014
 
Description National Science and Engineering Week 2013 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Geographic Reach Local
Primary Audience Schools
Results and Impact Lecture to Year 13 (age 17 to 18)"The fruitfly: a key model organism for understanding genetics and the brain", Hind Leys College. The talk sparked questions and discussion afterwards

no actual impacts realised to date
Year(s) Of Engagement Activity 2013
 
Description New Insights Into the Genetics of Diurnal/Nocturnal Preference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presentation at the conference of the Society for Research of Biological Rhythms. Palm Harbor, Florida May 2016
Year(s) Of Engagement Activity 2016
URL https://www.google.co.uk/url?sa=t&rct=j&q=&esrc=s&source=web&cd=6&cad=rja&uact=8&ved=0ahUKEwje4dv4pP...
 
Description New Walk Museum & Art Gallery lunch lecture 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact A lecture entitled: "What tiny fruit flies tell us about our genetics and the brain". The talk sparked questions and discussion afterwards.

no actual impacts realised to date
Year(s) Of Engagement Activity 2013
 
Description Oakham School science careers 07/03/14 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Meeting with Form 5-7 students at Oakham Schook and providing information on different possilbe careers in the Biosciences.

After my talk, the school asked if current GCSE pupils could visit my lab
Year(s) Of Engagement Activity 2014
 
Description Radio interview (Perth, Western Australia) 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact interview with 882 6PR Radio (Perth, Western Australia) covering our work on genetic differences associated with morningnenss/eveningnes preference in fruit-flies.
Year(s) Of Engagement Activity 2015
 
Description Role for DNA methylation in insect photoperiodic timing SRBR 2014 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact the talk sparked questions and discussion afterwards.

The impact has not been realised yet.
Year(s) Of Engagement Activity 2014
URL http://www.srbr.org/Pages/SRBR_Meeting.aspx
 
Description School Visit (Haifa) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Giving a lecture on using Drosophila as a model system in genetic research in the local high school. 50 pupils (Year 11) have attended. The school reported increased interest in the related subject area.
Year(s) Of Engagement Activity 2017
 
Description School visit: National Science & Engineering Week 2014 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach Local
Primary Audience Schools
Results and Impact The talk sparked questions afterwards.

After my talk, a few pupils asked for opportunity to work in my lab.
Year(s) Of Engagement Activity 2014
 
Description The diversity, evolution and mechanisms controlling activity patterns (Ein-Gedi, Israel) 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presentation at a workshop entitled: The diversity, evolution and mechanisms controlling activity patterns. Ein-Gedi, Israel

no actual impacts realised to date
Year(s) Of Engagement Activity 2012
URL http://campusteva.tau.ac.il/eng/content/diversity-evolution-and-mechanisms-controlling-activity-patt...