An International UK-Brazil Collaboration using Synchrotron Radiation Circular Dichroism Spectroscopy to Study Protein Structure and Function
Lead Research Organisation:
Birkbeck, University of London
Department Name: Biological Sciences
Abstract
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Publications
Felizatti AP
(2020)
Interactions of amphipathic a-helical MEG proteins from Schistosomamansoni with membranes.
in Biochimica et biophysica acta. Biomembranes
Kumagai PS
(2018)
Characterization of esterase activity from an Acetomicrobium hydrogeniformans enzyme with high structural stability in extreme conditions.
in Extremophiles : life under extreme conditions
Kumagai PS
(2019)
Unveiling the binding and orientation of the antimicrobial peptide Plantaricin 149 in zwitterionic and negatively charged membranes.
in European biophysics journal : EBJ
Lopes JL
(2013)
Folding factors and partners for the intrinsically disordered protein micro-exon gene 14 (MEG-14).
in Biophysical journal
Lopes JL
(2014)
Deconstructing the DGAT1 enzyme: Binding sites and substrate interactions.
in Biochimica et biophysica acta
Lopes JL
(2015)
Deconstructing the DGAT1 enzyme: membrane interactions at substrate binding sites.
in PloS one
Lopes JL
(2016)
Environmental Factors Modulating the Stability and Enzymatic Activity of the Petrotoga mobilis Esterase (PmEst).
in PloS one
Lopes JL
(2014)
Distinct circular dichroism spectroscopic signatures of polyproline II and unordered secondary structures: applications in secondary structure analyses.
in Protein science : a publication of the Protein Society
Lopes Jose Luiz S.
(2016)
Spectral Properties of "Disordered'' and Polyproline II Structures Defined by Circular Dichroism Spectroscopy
in BIOPHYSICAL JOURNAL
O'Reilly AO
(2014)
Chaperone-mediated native folding of a ß-scorpion toxin in the periplasm of Escherichia coli.
in Biochimica et biophysica acta
| Description | publications on collaborative studies and continued international collaboration |
| Exploitation Route | Brazil may develop their own SRCD beamline, with the advice of UK partners |
| Sectors | Agriculture, Food and Drink,Education,Pharmaceuticals and Medical Biotechnology |
| Description | this has directly lead to the design and building of an SRCD beamline at the new Brazil Synchrotron, and the Brazilian postdoc on this project (Yoneda) has recently been hired as its Beamline Scientist |
| First Year Of Impact | 2020 |
| Sector | Agriculture, Food and Drink,Chemicals,Education,Pharmaceuticals and Medical Biotechnology |
| Impact Types | Societal,Economic |
| Description | BBSRC-FAPSP partnering grant |
| Amount | £35,000 (GBP) |
| Funding ID | BB/N012763/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2016 |
| End | 12/2018 |
| Description | Binding and interactions of Brazilian toxins to voltage-gated sodium channels: The Rational Search for New Drugs |
| Amount | £12,648 (GBP) |
| Funding ID | BB/T01153X/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2020 |
| End | 01/2022 |
| Description | Biophysical studies of the structure/function of antimicrobial peptides and enzymes isolated from extremophile organisms |
| Amount | £35,426 (GBP) |
| Funding ID | BB/N012763/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2016 |
| End | 10/2018 |
| Description | Brazil Senior Visiting Fellowship |
| Amount | R$ 115,240 (BRL) |
| Organisation | National Council for Scientific and Technological Development (CNPq) |
| Sector | Public |
| Country | Brazil |
| Start | 03/2014 |
| End | 02/2016 |
| Description | Science without Borders Visiting Researcher |
| Amount | R$ 11,524,000 (BRL) |
| Organisation | National Council for Scientific and Technological Development (CNPq) |
| Sector | Public |
| Country | Brazil |
| Start | 03/2014 |
| End | 02/2016 |
| Description | Science without Borders psotdoctoral fellowships (3) |
| Amount | R$ 1 (BRL) |
| Organisation | National Council for Scientific and Technological Development (CNPq) |
| Sector | Public |
| Country | Brazil |
| Start | 09/2012 |
| End | 01/2016 |
| Description | Collaboration with University of Sao Paulo |
| Organisation | Universidade de São Paulo |
| Department | Physics Institute of São Carlos |
| Country | Brazil |
| Sector | Academic/University |
| PI Contribution | i have been involved in helping my brazilian collaborators prepare a proposal for an SRCD beamline at the Brazilian synchrotron |
| Collaborator Contribution | we have worked together on the proposal and planning |
| Impact | several publications, a long term partnership leading to several BBSRC partnering grants, development of a new SRCD beamline at the Brazilian synchrotron, submission and funding of a new BBSRC partnering award |
| Start Year | 2012 |
| Description | collaborations with CD users |
| Organisation | Beijing Synchotron Radiation Facility |
| Country | China |
| Sector | Academic/University |
| PI Contribution | cd data collection and analyses |
| Collaborator Contribution | beamtime made available |
| Impact | publications |
| Start Year | 2013 |
| Description | Running training workshops for students, postdocs and industrial scientists on CD spectroscopy |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Teaching Lectures and Practicals in multi-day workshops to provide new skills training and ideas and information for researchers both nationally and internationally |
| Year(s) Of Engagement Activity | Pre-2006,2006,2007,2008,2009,2010,2011,2012,2013,2014,2015,2016,2017,2018 |
| Description | Talks at UK and international sites and meetings: Brazil Synchrotron Symposium, ), University of Vienna Austria (Chemistry Dept), Biochemical Society Training Course (Aston University, UK) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Talks about Tools and Resources developed in the course of this grant at Universities and International meetings, including the headline talk at the inauguration of the Brazil Synchrotron SRCD beamline (CEDRO) held for the international community of SRCD users and potential users, The Biochemical Society training course on Membrane Proteins at Aston University, the chemistry dept at the University of Vienna. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Teaching workshops for students, faculty and industrial scientists |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | A series of workshops on methods of analysis for CD spectroscopy given at national and international meetings and workshops, usually 2-3 days, including hands-on computing practicals. for phd and masters students, postdocs, professors and other academics and industry |
| Year(s) Of Engagement Activity | 2009,2010,2011,2012,2013,2014,2015,2016,2017,2018 |
| Description | annual talks for the public |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | UCL Science Centre Friday Evening Discourses and Birkbeck SET Talks for the Public talks on science for the public (5th, 6th form, and public) annually and lecture/tour at the Wellcome Collection talks no actual impacts realised to date |
| Year(s) Of Engagement Activity | 2006,2007,2008,2009,2010,2011,2012,2013,2014,2015,2016,2017,2018,2019,2020,2021 |
| Description | talks at national and international meetings |
| Form Of Engagement Activity | Scientific meeting (conference/symposium etc.) |
| Part Of Official Scheme? | No |
| Type Of Presentation | paper presentation |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | in UK, USA, Australia, Austria, Belgium, Taiwan, Denmark, France, Germany, China, Brazil, Japan: talks at international meetings and workshops established new collaborations |
| Year(s) Of Engagement Activity | Pre-2006,2006,2007,2008,2009,2010,2011,2012,2013,2014 |