Controlling dormancy and sprouting in potato and onion

Lead Research Organisation: Imperial College London
Department Name: Life Sciences

Abstract

Potato and onion are major UK and worldwide crops required year-round by consumers and processors. Due to seasonal production, long term storage is necessary, during which produce must be maintained with good quality for fresh consumption and processing, and in a nutritious state. Potato tubers and onion bulbs are natural over-wintering structures with a tendency to resume growth during storage, resulting in sprouted produce that is unattractive and unsaleable, or unsuited to processing due to compositional changes such as increased sugar levels. Multiple strategies are used to extend dormancy and minimise sprouting and waste, including low temperature storage and application of sprout suppressants such as chlorpropham, maleic hydrazide or ethylene. Such treatments are not fully effective as quality deterioration may occur even if sprouting is inhibited and legislation increasingly limits use of many of these chemicals. In addition, long-term cold storage is a major economic cost with a substantial carbon footprint.

Development of alternative strategies to maintain tubers and bulbs in a dormant state and long-term suppression of sprouting are top industry priorities. Genetic studies in potato have shown that inheritance of tuber dormancy characteristics is affected by several genes acting alone or in combination, but the identity of these genes is unknown. Despite substantial progress, a full understanding of the biology of dormancy and sprouting has not yet emerged, and this substantially hampers development of new strategies for storage, and breeding of new varieties with better dormancy and sprouting behaviours. Fortunately recent advances in the field of molecular biology allow us to make major advances to address these issues.

Scientific studies have revealed common roles in potato and onion for several plant hormones including abscisic acid, ethylene, gibberellins and cytokinins, in regulation of dormancy, and sprout growth, suggesting that knowledge of one commodity further our understanding of another. This project will benefit from major advances in potato genetics, especially publication of the genome sequence, as well as huge developments in DNA sequencing technologies which now enable in-depth analysis of the relatively unexplored but highly complex onion genome. New, powerful potato genetic resources will allow us to pinpoint the position and identity of genes that exert the greatest control of dormancy and sprouting. These resources include large mapping populations, developed by crossing highly divergent parents. Preliminary studies have already revealed genomic regions containing key genes that can drive crop improvement and new management methods.

The assembled research consortium brings together James Hutton Institute, Cranfield University, Imperial College London and Greenwich University, providing a wealth of experience in genomics, genetics, molecular biology, physiology, agronomy and storage of potato and onion.

Project outcomes will include (1) identification of key genes in potato and onion, their variant forms and regulatory mechanisms that underpin potato tuber dormancy, (2) development of genome-wide data on major genes in onion bulb dormancy and sprouting, and (3) comparison of shared and distinctive elements of dormancy and sprouting control in potato and onion, leading to elucidation of key physiological and molecular control steps.

Through involvement of industry representative bodies and companies, information generated can readily be translated towards enhanced, variety-specific storage regimes, enabling reduced chemical usage and less reliance on expensive low temperature storage. Knowledge of key regulatory genes can in the longer term be adopted by breeders to develop potatoes with better dormancy characteristics.

Technical Summary

This science-led project addresses a major commercial problem in potato and onion industries, namely loss of crop value and quality due to untimely postharvest sprouting from tubers and bulbs. Chemical controls are widely deployed but many of these may be withdrawn and others are not universally effective. Alternative routes to preventing sprouting are therefore urgently needed. Duration of meristem endodormancy and subsequent rate of sprout extension are key traits for which substantial genotypic variation exists, and some of this has been associated with QTLs in potato. However, the underlying genes are not yet defined, nor is the substantial influence of pre- and post-harvest environment fully understood.

We have therefore assembled a team with complementary expertise across genetics, molecular biology, physiology and storage of both species. We will take advantage of unique potato genetic resources, especially substantial mapping populations with diversity in dormancy, and the recent availability of a high quality genome sequence. Onion genomics are much less advanced, but we will access the latest resources and generate novel RNAseq data. This will allow innovative species and genotype comparisons

Our starting point is a working model of hormone signalling in regulation of dormancy and post-dormancy growth; this is based on our data for cytokinins, strigolactones, ethylene and ABA. We will advance and test this model by sampling extreme genotypes from both narrow genetic bases (individuals from mapping populations) and broader potato and onion diversity. By accurately defining duration of endodormancy and tracing transcript and hormone profiles in tuber and meristem tissues throughout crop development and post-harvest storage, we will extract the strongest variables that robustly correlate with, predict and/or regulate dormancy status. Close alignment with industry will enable translation and further testing of models under commercial conditions.

Planned Impact

Who might benefit from this research?
This proposal has been developed in consultation with key UK industrial beneficiaries. Enhanced knowledge and tools relating to potato storage will benefit all industry sectors, reflected by the financial support of the Potato Council, who include this topic as a priority area in their R&D strategy. Direct beneficiaries include PepsiCo PLC, a global company with substantial activity in the UK processing potato market, and Albert Bartlett, UK's leading grower and packer of potatoes (25% market share of UK fresh & seed potato production) Collectively the levy board and industrial partners have committed significant funding to the project (10% cash).

Ultimately project outcomes will impact on sustainable food production. UK food self-sufficiency will result in reduced imports and costs to consumers. In the global economy, improved human nutrition anywhere will improve global security and thereby benefit the UK. Outcomes of this research will also impact on breeding in developing countries. For example the International Potato Centre (CIP) operates several breeding programmes for Asia and Africa, where crop storage facilities are limited. A unified model of dormancy control across crops included here will provide impetus for research on less studied staples (yam, sweet potato) vital for food security in some of world's poorest regions.

How might they benefit from this research?
Currently in the UK, storage waste for potatoes and onions is between 3-25%. Much of this waste is related to breaking of endormancy and premature sprouting. Chemical inhibitors of sprouting (e.g. chlorpropham (CIPC) and maleic hydrazide (MH)) are UK industry standards; however they are under severe threat of being withdrawn under EU regulation, since they are increasingly considered undesirable by consumers and regulators. Currently, of the 4.05 million tonnes of potatoes stored annually in the UK, 44% is treated with CIPC, so there is an urgent need to develop new storage strategies for potato and onion, less reliant on CIPC (please see letter of support from CIPC stewardship group). This new consortium will employ cutting-edge technologies to develop genetic, molecular, biochemical markers in potato and onion for evaluation of dormancy status in existing and emerging cultivars and to understand the impact of agronomic/storage practices on the development of dormancy.

For onion, our proposed research and assemblies will feed directly into the current international efforts on transcriptome analysis for onion and other alliums. With the assistance of Prof. Havey of the University of Wisconsin we will ensure that we integrate and make public onion transcriptome data.

Longer term, plant breeding is the route through which almost all genetic advances in crop production will benefit the wider community. The commercial arm of JHI, Mylnefield Research Services, runs potato breeding programmes for all major UK potato producers, enabling ready routes to translate research outcomes to industry.

Wide dissemination will ensure full benefit of project outcomes. Participation of the Potato Council in the consortium is pivotal through its links across the UK potato industry. The Potato Council will also provide feedback from industry on the project and the uptake of its outcomes. Similarly, established links between CU and the British Onion Producers Association will provide a route to interaction with the onion industry.

NRI-UoG works closely with international research organisations including CGIAR centres such as CIP, IITA and CIAT to support research on root crops in order to improve food security worldwide. It is therefore well placed to ensure that project outputs are fully exploited through breeding and technology development to improve storage and reduce losses for potato, onion and other root crops.

Publications

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Description This research was conducted in close collaboration with our academic and industry partners within the consortium, with funding period completed in 2017. The Imperial College component has focussed extensively on molecular and biochemical changes that occur during potato dormancy break, and that also are found in comparisons between high dormancy and low dormancy potato types. By sampling from potatoes grown across multiple seasons, we built up a detailed picture of the changes that are consistently associated with dormancy. These include genes that appear to be switched on or off, and signalling molecules (hormones) that either increase or decrease depending on dormancy status. In the final research phase, we demonstrated that what we have learned from test populations of potato that exhibit high variation in dormancy, can be translated to commercial potato varieties. There may be potential for applications in developing markers for aiding selection during breeding and/or markers as tools to aid crop management pre- and/or post-harvest.
Exploitation Route Aimed at potato and onion industry issue of improving storage life through better genetics and crop managements, ideally using fewer or no chemicals.
Sectors Agriculture, Food and Drink

 
Description The first part of the project tested dormancy variation in controlled (non-commercial) potato populations developed by James Hutton Institute. In the final 2 years of the project, we ran comparisons with commercial materials (named potato varieties) provided by our industry partners. We conclude that the model and data based on non-commercial test populations from James Hutton Institute show very similar molecular profiles to the tested commercial potato varieties. Industry partners on the project are presently considering options for take-up.
First Year Of Impact 2017
Sector Agriculture, Food and Drink
 
Description Cranfield 
Organisation Cranfield University
Department Plant Science Group
Country United Kingdom 
Sector Academic/University 
PI Contribution Partner organisation in BBSRC HAPI funded collaboration.
Collaborator Contribution We work closely with all partners in this BBSRC funded collaboration
Impact none to date
Start Year 2013
 
Description Greenwich 
Organisation University of Greenwich
Country United Kingdom 
Sector Academic/University 
PI Contribution Partner in BBSRC HAPI funded collaboration
Collaborator Contribution We work closely with all partners in this BBSRC funded collaboration
Impact none to date
Start Year 2013
 
Description James Hutton Institute 
Organisation James Hutton Institute
Country United Kingdom 
Sector Public 
PI Contribution JHI is lead partner in BBSRC HAPI collaborative project "Controlling dormancy and sprouting in potato and onion
Collaborator Contribution We work closely together on all aspects of the funded research programs, aiming at defining the molecular basis of dormancy regulation in potato.
Impact none to date
Start Year 2013