US partnering on the use of neutron scattering to study aggregation in therapeutic proteins during manufacture and storage
Lead Research Organisation:
University College London
Department Name: Biochemical Engineering
Abstract
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Publications
Codina N
(2019)
An Expanded Conformation of an Antibody Fab Region by X-Ray Scattering, Molecular Dynamics, and smFRET Identifies an Aggregation Mechanism.
in Journal of molecular biology
Mazzer AR
(2015)
Protein A chromatography increases monoclonal antibody aggregation rate during subsequent low pH virus inactivation hold.
in Journal of chromatography. A
Mazzer AR
(2017)
Neutron reflectivity measurement of protein A-antibody complex at the solid-liquid interface.
in Journal of chromatography. A
Papachristodoulou M
(2020)
In situ neutron scattering of antibody adsorption during protein A chromatography
in Journal of Chromatography A
Rayner LE
(2015)
The solution structures of two human IgG1 antibodies show conformational stability and accommodate their C1q and Fc?R ligands.
in The Journal of biological chemistry
| Description | SANS measurements at ISIS Oxford, UK were carried out by a PDRA of Chris Roberts from Delaware, US. SAXS measurements were carried out at NIST, US, by a UCL PDRA. These measurements have revealed structural changes in antibodies as they adhere to chromatographic resin surfaces. |
| Exploitation Route | This work will lead to publications and also to a new grant application to extend the work in more detail. |
| Sectors | Healthcare |
| Description | This work has opened up a dialogue with NIST scientists working also on biopharmaceutical measurements. NIST have now promised to supply materials for a new EPSRC grant application in the UK. The work has also led to a collaborative EPSRC CDT PhD with UCL (Dalby), Chris Roberts in Delaware, and Univ Manchester (Curtis). The work at ISIS has also enabled a jointly funded UCL EPSRC CDT PhD project to launch in Oct 2016, to investigate glycosylation structures in antibodies in solution. Two conferences have also been organised as a result of this collaborative project. 1. Neutron Characterisation in Fundamental and Applied Biotechnology, Abingdon, UK, September 2014 2. Advances in Biophysical Methods for Protein Characterisation, Palermo, Italy, October 2016. |
| First Year Of Impact | 2016 |
| Sector | Healthcare |
| Impact Types | Economic |