Linking c-di-GMP signalling and the Gac/Rsm signal transduction pathway
Lead Research Organisation:
Imperial College London
Department Name: Life Sciences
Abstract
Pseudomonas aeruginosa is a gram-negative bacterium that thrives in a multitude of environmental niches including soil, moist surfaces or plants. It is also an opportunistic human pathogen causing infections associated with high levels of morbidity and mortality. Epidemiological studies have shown that no significant differences in the virulence potential of clinical or environmental isolates could be observed, which suggests that the available arsenal of virulence factors might also be useful to fight eukaryotic predators that may be encountered in the environment (e.g. protozoans and metazoans). As such, P. aeruginosa is a versatile organism and its adaptive potential to fluctuating environmental cues relies on numerous and complex signalling pathways. These pathways determine whether P. aeruginosa adopts a motile/toxic or sessile/biofilm lifestyle. These lifestyles directly relate to acute or chronic infection mode.
The decision making process for choosing in between lifestyles is governed by major regulatory pathways which are antagonistic but also cross-talk via positive and negative feedback loops. These involve Quorum Sensing, or the second messenger molecule c-AMP, which are both master regulators for promoting production of bacterial virulence factors such as toxins. In contrast the so-called, Gac/Rsm pathway, which involves molecular sensors for probing environmental conditions, and the second messenger molecule c-di-GMP are main players to control the formation of biofilm.
A biofilm is a bacterial population attached on a surface and held together by a sticky extracellular matrix made of polysaccharide, DNA or adhesive proteins. Bacteria embedded in such matrix form a very stable population which in the environment can resist various stresses such as the current of a river flow when attached on rocks, UV irradiation, dehydration or nutrient starvation for example. For pathogens, once the bacteria establish as a biofilm in one particular tissue or organ, such as in the lungs of cystic fibrosis patients in case of P. aeruginosa, it is basically impossible to eradicate. The population became highly resistant to attack by the immune system or to antibiotic therapy. This way a chronic and persistent infection establishes and morbidity reaches a very high level.
In our project we will investigate the intimate connections that may exist in between the formation/dispersion of a P. aeruginosa biofilm and the two regulatory pathways Gac/Rsm on the one hand and c-di-GMP on the other hand. We want to build on our expertise and pioneering work in the characterization of the Gac/Rsm pathway and on our recent demonstration that this route intersects with the c-di-GMP signaling pathway. We want to establish the molecular links between these two main players and understand how it may then act on the formation of the biofilm and beyond this how it exerts an antagonistic action on the production of virulence factors such as toxins. Indeed, these regulatory pathways are at the intersection of a Doctor Jekyll and Mister Hyde game, in which bacteria hesitate to balance either towards an aggressive and toxic lifestyle or towards a more silent and insidious lifestyle under the form of a biofilm.
In conclusion, the regulatory network we will investigate is not unique to the human pathogen P. aeruginosa but common to most gram-negative bacteria for c-di-GMP and to most Pseudomonas species for Gac/Rsm including plant growth promoters (Pseudomonas putida and Pseudomonas fluorescens) or plant pathogens (Pseudomonas syringae). We believe that a precise molecular understanding of this circuitry will allow to develop small molecules designed for manipulating the fate of bacteria, subverting and influencing their decision making process to our own benefit. This study will have strong implications in several areas including, Ecology and Agriculture (niche/root colonization), Medical and Therapeutic approaches and Fundamental and Basic Sciences.
The decision making process for choosing in between lifestyles is governed by major regulatory pathways which are antagonistic but also cross-talk via positive and negative feedback loops. These involve Quorum Sensing, or the second messenger molecule c-AMP, which are both master regulators for promoting production of bacterial virulence factors such as toxins. In contrast the so-called, Gac/Rsm pathway, which involves molecular sensors for probing environmental conditions, and the second messenger molecule c-di-GMP are main players to control the formation of biofilm.
A biofilm is a bacterial population attached on a surface and held together by a sticky extracellular matrix made of polysaccharide, DNA or adhesive proteins. Bacteria embedded in such matrix form a very stable population which in the environment can resist various stresses such as the current of a river flow when attached on rocks, UV irradiation, dehydration or nutrient starvation for example. For pathogens, once the bacteria establish as a biofilm in one particular tissue or organ, such as in the lungs of cystic fibrosis patients in case of P. aeruginosa, it is basically impossible to eradicate. The population became highly resistant to attack by the immune system or to antibiotic therapy. This way a chronic and persistent infection establishes and morbidity reaches a very high level.
In our project we will investigate the intimate connections that may exist in between the formation/dispersion of a P. aeruginosa biofilm and the two regulatory pathways Gac/Rsm on the one hand and c-di-GMP on the other hand. We want to build on our expertise and pioneering work in the characterization of the Gac/Rsm pathway and on our recent demonstration that this route intersects with the c-di-GMP signaling pathway. We want to establish the molecular links between these two main players and understand how it may then act on the formation of the biofilm and beyond this how it exerts an antagonistic action on the production of virulence factors such as toxins. Indeed, these regulatory pathways are at the intersection of a Doctor Jekyll and Mister Hyde game, in which bacteria hesitate to balance either towards an aggressive and toxic lifestyle or towards a more silent and insidious lifestyle under the form of a biofilm.
In conclusion, the regulatory network we will investigate is not unique to the human pathogen P. aeruginosa but common to most gram-negative bacteria for c-di-GMP and to most Pseudomonas species for Gac/Rsm including plant growth promoters (Pseudomonas putida and Pseudomonas fluorescens) or plant pathogens (Pseudomonas syringae). We believe that a precise molecular understanding of this circuitry will allow to develop small molecules designed for manipulating the fate of bacteria, subverting and influencing their decision making process to our own benefit. This study will have strong implications in several areas including, Ecology and Agriculture (niche/root colonization), Medical and Therapeutic approaches and Fundamental and Basic Sciences.
Technical Summary
Microorganisms developed strategies to adapt to changes in their surrounding using regulatory cascades. Pseudomonas aeruginosa is a versatile bacterium with high adaptive potential, which is found in most environments and also a nosocomial pathogen.
P. aeruginosa thrives as a motile organism, i.e. planktonic lifestyle, or establish a biofilm, i.e. sessile lifestyle. Biofilm is associated with persistent infection while planktonic lifestyle correlates with systemic dissemination. We contributed the characterization of a network, which is a key switch in P. aeruginosa lifestyles. It involves two sensors, LadS and RetS, which antagonistically influence the Gac/Rsm pathway. Gac/Rsm controls level of small RNAs, which sequester the repressor RsmA. As a consequence biofilm formation is promoted and motility is reduced. RsmA sequestration also causes a switch in proteins secretion systems with an increase in T6SS, whereas the cytotoxic activity of the T3SS is shut down.
In recent years we added complexity by showing that i) another regulatory pathway centred on the phosphorelay HptB runs parallel to RetS/LadS but intersects with Gac/Rsm and ii) that c-di-GMP, a universal signalling molecule for biofilm formation, is connected to Gac/Rsm.
We aim at understanding the molecular connections existing between the signal transduction pathways involving RetS and HptB and c-di-GMP signalling. Signalling cascades involving c-di-GMP are poorly understood and c-di-GMP binding proteins are of different types. It is predicted that the transfer of c-di-GMP from the biosynthetic enzymes to cognate effectors may involve a cascade of protein-protein interactions. We will use genetic and biochemical approaches to elucidate the link between Gac/Rsm and c-di-GMP, which will be our case study. We shall also bring further light on c-di-GMP signalling in general by using a bacterial two-hybrid library of P. aeruginosa and probing protein-protein interactions with c-di-GMP metabolic enzymes.
P. aeruginosa thrives as a motile organism, i.e. planktonic lifestyle, or establish a biofilm, i.e. sessile lifestyle. Biofilm is associated with persistent infection while planktonic lifestyle correlates with systemic dissemination. We contributed the characterization of a network, which is a key switch in P. aeruginosa lifestyles. It involves two sensors, LadS and RetS, which antagonistically influence the Gac/Rsm pathway. Gac/Rsm controls level of small RNAs, which sequester the repressor RsmA. As a consequence biofilm formation is promoted and motility is reduced. RsmA sequestration also causes a switch in proteins secretion systems with an increase in T6SS, whereas the cytotoxic activity of the T3SS is shut down.
In recent years we added complexity by showing that i) another regulatory pathway centred on the phosphorelay HptB runs parallel to RetS/LadS but intersects with Gac/Rsm and ii) that c-di-GMP, a universal signalling molecule for biofilm formation, is connected to Gac/Rsm.
We aim at understanding the molecular connections existing between the signal transduction pathways involving RetS and HptB and c-di-GMP signalling. Signalling cascades involving c-di-GMP are poorly understood and c-di-GMP binding proteins are of different types. It is predicted that the transfer of c-di-GMP from the biosynthetic enzymes to cognate effectors may involve a cascade of protein-protein interactions. We will use genetic and biochemical approaches to elucidate the link between Gac/Rsm and c-di-GMP, which will be our case study. We shall also bring further light on c-di-GMP signalling in general by using a bacterial two-hybrid library of P. aeruginosa and probing protein-protein interactions with c-di-GMP metabolic enzymes.
Planned Impact
Pseudomonas aeruginosa is a major cause of hospital-acquired infection, with an estimated 10,000 cases each year in UK. Infection is severe and life-threatening, leading to pneumonia or septicaemia. P. aeruginosa is also dreaded by cystic fibrosis patients. Up to 80% carry the bacteria in their lungs and infection is proving fatal.
Regulation of P. aeruginosa gene expression in relation with biofilm formation and chronic infection is an intense field of research involving top class laboratories all over the world. Together with Prof Stephen Lory's laboratory (Harvard Medical School), my laboratory has been a major player in this area. Our discovery of bacterial sensors that switch on and off genes involved in virulence and biofilm formation, in an antagonistic manner, has been considered as a seminal finding in this area of research and is of major interest for the academic community as well as for clinicians and pharmaceutical companies. The sensors we discovered, LadS and RetS, influence the activity of the Gac/Rsm signalling pathway, which has been described in several other organisms. Our more recent work demonstrates that the universal c-di-GMP second messenger is tightly connected to the Gac/Rsm pathway to signal and decide on the bacterial behaviour. Although our research is basic research, molecular understanding of infection strategy employed by such an important human pathogen may lead to biomedical applications, new antimicrobials and societal impact. Furthermore the existence of a similar pathway in the plant pathogen Pseudomonas syringae will not restrict applications to human pathogens and may thus have impact in agriculture as well. Finally the pathway is crucial in typically environmental organisms such as Pseudomonas putida or Pseudomonas fluorescens and certainly influences their ability to colonize their environmental niches which suggest that understanding this regulatory network will have broader impact on understanding the fate of microbial population in nature and thus on ecology.
Based on the visibility of my laboratory in the field and a broad network of collaborators, it will be another achievement to organize a small size (50 participants) meeting on the site of Imperial College (near the end of the project). The topic will be "Regulation of gene expression in P. aeruginosa, biofilm and chronic infection". It will attract the main UK players in this field (Nottingham University, Cambridge University, Southampton University) together with other groups from France (CNRS Marseille, Institut Pasteur) and US (Harvard Medical School, University of Washington, Binghamton University). This way we will be in control of disseminating our findings not only by attending regular conferences and by publishing in high impact factor journals. Pharmaceutical companies representative (Sanofi-Aventis), and journal editors (Nature) will also be invited to attend the conference. Alain Filloux has a good expertise in conference organization, having been the organizer of the Pseudomonas meeting (Marseille 2005) and chair of the Gordon conference on Microbial adhesion and cell signalling (Newport 2009).
Regulation of P. aeruginosa gene expression in relation with biofilm formation and chronic infection is an intense field of research involving top class laboratories all over the world. Together with Prof Stephen Lory's laboratory (Harvard Medical School), my laboratory has been a major player in this area. Our discovery of bacterial sensors that switch on and off genes involved in virulence and biofilm formation, in an antagonistic manner, has been considered as a seminal finding in this area of research and is of major interest for the academic community as well as for clinicians and pharmaceutical companies. The sensors we discovered, LadS and RetS, influence the activity of the Gac/Rsm signalling pathway, which has been described in several other organisms. Our more recent work demonstrates that the universal c-di-GMP second messenger is tightly connected to the Gac/Rsm pathway to signal and decide on the bacterial behaviour. Although our research is basic research, molecular understanding of infection strategy employed by such an important human pathogen may lead to biomedical applications, new antimicrobials and societal impact. Furthermore the existence of a similar pathway in the plant pathogen Pseudomonas syringae will not restrict applications to human pathogens and may thus have impact in agriculture as well. Finally the pathway is crucial in typically environmental organisms such as Pseudomonas putida or Pseudomonas fluorescens and certainly influences their ability to colonize their environmental niches which suggest that understanding this regulatory network will have broader impact on understanding the fate of microbial population in nature and thus on ecology.
Based on the visibility of my laboratory in the field and a broad network of collaborators, it will be another achievement to organize a small size (50 participants) meeting on the site of Imperial College (near the end of the project). The topic will be "Regulation of gene expression in P. aeruginosa, biofilm and chronic infection". It will attract the main UK players in this field (Nottingham University, Cambridge University, Southampton University) together with other groups from France (CNRS Marseille, Institut Pasteur) and US (Harvard Medical School, University of Washington, Binghamton University). This way we will be in control of disseminating our findings not only by attending regular conferences and by publishing in high impact factor journals. Pharmaceutical companies representative (Sanofi-Aventis), and journal editors (Nature) will also be invited to attend the conference. Alain Filloux has a good expertise in conference organization, having been the organizer of the Pseudomonas meeting (Marseille 2005) and chair of the Gordon conference on Microbial adhesion and cell signalling (Newport 2009).
People |
ORCID iD |
Alain Filloux (Principal Investigator) |
Publications
Allsopp LP
(2017)
RsmA and AmrZ orchestrate the assembly of all three type VI secretion systems in Pseudomonas aeruginosa.
in Proceedings of the National Academy of Sciences of the United States of America
Aragon IM
(2015)
Diguanylate cyclase DgcP is involved in plant and human Pseudomonas spp. infections.
in Environmental microbiology
Bouffartigues E
(2015)
The absence of the Pseudomonas aeruginosa OprF protein leads to increased biofilm formation through variation in c-di-GMP level.
in Frontiers in microbiology
Evans L
(2023)
Identification and characterisation of G-quadruplex DNA-forming sequences in the Pseudomonas aeruginosa genome.
in RSC chemical biology
Jakobsen TH
(2017)
A broad range quorum sensing inhibitor working through sRNA inhibition.
in Scientific reports
Larrouy-Maumus G
(2016)
Direct detection of lipid A on intact Gram-negative bacteria by MALDI-TOF mass spectrometry.
in Journal of microbiological methods
McCarthy RR
(2019)
Cyclic di-GMP inactivates T6SS and T4SS activity in Agrobacterium tumefaciens.
in Molecular microbiology
McCarthy RR
(2017)
Contribution of Cyclic di-GMP in the Control of Type III and Type VI Secretion in Pseudomonas aeruginosa.
in Methods in molecular biology (Clifton, N.J.)
McCarthy RR
(2017)
Cyclic-di-GMP regulates lipopolysaccharide modification and contributes to Pseudomonas aeruginosa immune evasion.
in Nature microbiology
Moscoso JA
(2014)
The diguanylate cyclase SadC is a central player in Gac/Rsm-mediated biofilm formation in Pseudomonas aeruginosa.
in Journal of bacteriology
Description | - We have identified that the cyclase SadC plays a key role in the Gac/Rsm regulatory network and the P. aeruginosa lifestyle switch (Moscoso JA, Jaeger T, Valentini M, Hui K, Jenal U, Filloux A. J Bacteriol. 2014 Dec 1;196(23):4081). - We have identified that the cyclase PA3343 is central to the HptB signalling pathway and plays a key role in P. aeruginosa motility. This cyclase has been called HsbD and is controlling motility behaviors temporally and spatially. Collaboration with Urs Jenal (basel) allow to visualize the cyclase colaocalisig tha the cell pole together with the flagellum. We have published a paper in PLoS Genetics presenting these data: Martina Valentini, Benoît-Joseph Laventie, Joana Moscoso, Urs Jenal and Alain Filloux. - We have identified interacting partners for the cyclase SadC using a systematic bacterial two-hybrid screen. One of the hit is an enzyme involved in LPS biogenesis and thus involve in modifying surface properties of the bacterium and thus antigenicity. We called the enzyme WarA and demonstrated it has methyltransferase activity. We have collaborated with Serge Mostowy to show how this modification changes the ability of the bacterium to escape the immune system using Zebra fish as infection model. This work has been published in Nature Microbiology (Mc Carthy, R., Mazon-Moya, M. J., Moscoso, J. A., Lam, J., Hao, Y., Bordi, C., Mostowy, S. and Filloux, A. (2017) Cyclic di-GMP regulates lipopolysaccharide modifications and contributes to Pseudomonas aeruginosa immune evasion. Nature Microbiology 2: 17027) - We have identified a connection between c-di-GMP signalling and protein secretion systems (T6SS and T4SS) in A. tulemaciens which adds up to our findings about the connection between c-di-GMP and T6SS/T3SS in pseudomonas aeruginosa (McCarthy, R.R, Yu, M., Eilers, K., Wang, Y-C., Lai, E-M. and Filloux, A. (2019) Cyclic di-GMP inactivates T6SS and T4SS activity in Agrobacterium tumefaciens. Mol. Microbiol. 112(2):632-648) |
Exploitation Route | Understanding c-di-GMP signalling in bacterial pathogens may influence their ability to form biofilm and help to develop therapeutic strategy to prevent persistent and chronic infections. We are currently developing further collaboration on this work in the context of the CF Trust-funded Strategic Research Center (Loren Cameron, PhD and co-supervision Jane Davies) by investigating the role of c-di-GMP in clinical isolates, as well as understanding the link between c-di-GMP and antibiotic resistance in the context of the ARC fellowship obtained by Martina Valentini. We have engaged collaboration with the Drug Discovery Unit in Dundee to identify compounds that will inhibit the methyltransferase activity of WarA and thus favour immune recognition. |
Sectors | Education Healthcare Pharmaceuticals and Medical Biotechnology |
Description | International Centre for Infectiology Research" (CIRI, Lyon, France) |
Geographic Reach | Europe |
Policy Influence Type | Participation in a guidance/advisory committee |
Description | BBSRC DTP Studentship to Marta Rudzite |
Amount | £85,000 (GBP) |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 09/2019 |
End | 10/2022 |
Description | BBSRC Research grant - A bacterial c-di-GMP responsive enzyme modulates LPS structure and triggers immune evasion |
Amount | £486,413 (GBP) |
Funding ID | BB/R00174X/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 02/2018 |
End | 01/2021 |
Description | Personalised Approach to Pseudomonas aeruginosa (PAPA) - CF Trust SRC |
Amount | £750,000 (GBP) |
Funding ID | SRC 014 |
Organisation | Cystic Fibrosis Trust |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 08/2018 |
End | 09/2021 |
Description | Pseudomonal infection in CF: better detection, better understanding, better treatment |
Amount | £745,708 (GBP) |
Funding ID | SRC001 |
Organisation | Cystic Fibrosis Trust |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 02/2015 |
End | 08/2018 |
Description | Swiss National Science Foundation fellowship (SNSF) to Martina Valentini - 01/01/13 to 30/06/15 |
Amount | SFr. 72,000 (CHF) |
Organisation | Swiss National Science Foundation |
Sector | Public |
Country | Switzerland |
Start | 01/2013 |
End | 06/2015 |
Description | Wellcome Trust Institutional strategic support fund - Antimicrobial Research Collaborative (ARC) Early Career Research Fellowships to Martina Valentini |
Amount | £57,832 (GBP) |
Organisation | Imperial College London |
Sector | Academic/University |
Country | United Kingdom |
Start | 09/2015 |
End | 09/2016 |
Title | Antibodies against OSA and CPA LPS from Pseudomonas aeruginosa |
Description | Antibodies against OSA and CPA LPS from Pseudomonas aeruginosa |
Type Of Material | Antibody |
Provided To Others? | Yes |
Impact | Antibodies that specifically detect either type of Pseudomonas aeruginosa LPS |
Description | Antibodies against CPA and OSA LPS types from Pseudomonas aeruginosa |
Organisation | University of Guelph |
Department | Department of Molecular and Cellular Biology |
Country | Canada |
Sector | Academic/University |
PI Contribution | We used provided antibodies to probe LPS modifications in Pseudomonas aeruginosa warA or sadC mutants, as well as in P. aeruginosa strains overexpressing sadC. |
Collaborator Contribution | Professor Joe Lam provide antibodies against Pseudomonas aeruginosa LPS. |
Impact | A paper was published in Nature Microbiology in 2017. Cyclic-di-GMP regulates lipopolysaccharide modification and contributes to Pseudomonas aeruginosa immune evasion. McCarthy RR, Mazon-Moya MJ, Moscoso JA, Hao Y, Lam JS, Bordi C, Mostowy S, Filloux A. Nat Microbiol. 2017 Mar 6;2:17027. doi: 10.1038/nmicrobiol.2017.27. |
Start Year | 2015 |
Description | Collaboration Dr Serge Mostowy (Imperial College London) |
Organisation | Imperial College London |
Department | Faculty of Medicine |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We will provide Pseudomonas aeruginosa mutant strains to assess virulence in the Zebra fish model |
Collaborator Contribution | Dr Mostowy will perform the infections and provide data including live imaging microscopy. |
Impact | Preliminary data have identified some of our mutants (warA and sadC) as being poorly targeted by the Zebra fish macrophages. |
Start Year | 2015 |
Description | Collaboration Professor Aras Kadioglu (Liverpool University) |
Organisation | University of Liverpool |
Department | Institute of Infection and Global Health |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Professor Kadioglu has extensive expertise for working with mice models of infection using Pseudomonas aeruginosa, and we will provide a large set of mutant strains to assess their virulence. |
Collaborator Contribution | Professor Kadioglu will perform the infections and collect the data. |
Impact | Collaboration just started and we have provided the strains |
Start Year | 2015 |
Description | Drug Discovery Unit - University of Dundee |
Organisation | University of Dundee |
Department | Drug Discovery Unit |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We identified a methyltransferase, WarA, which is activated by SadC and c-di-GMP, which in turn modifies the LPS surface composition. The inactivation of warA results in immediate recognaition by neutrophils in the zebra fish infection model. This work has been published in Nature Microbiology and will appera online on March 6th 2017. |
Collaborator Contribution | The collaboration with DDU is to identify compounds that may incativate WarA methyltransferas activity. |
Impact | None yet |
Start Year | 2017 |
Description | Visiting Professor - Nanyang Technical University - SCELSE - Singapore |
Organisation | Nanyang Technological University |
Country | Singapore |
Sector | Academic/University |
PI Contribution | Visiting Professor since 2018 and effective collaboration with the group of Prof Michael Givskov. Laura Nolan visited also the Centre in April 2018 to implement the TraDIS methodology. We have now engineered a collection of mutants affected in all known genes encoding enzymes making or breakinfg c-di-GMP |
Collaborator Contribution | Hosted Laura Nolan for supporting approaches using the TraDIS methodology. Our colaborator at SCELSE is now analysing the biofilm phenotype of all of our c-di-GMP related mutants using conofcal microsopy and biofilm grown in flow cells. |
Impact | None yet |
Start Year | 2018 |
Description | A talk at NTU in Singapore by Prof Alain Filloux |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Talk by the PI Alain Filloux to PI, Postdoctoral fellows and PhD students from the SCELSE Institute at the NTU in Singapore. Role of c--di-GMP in biofilm formation and immune evasion and hos it relates to the central regulatory network RsmA/SadC |
Year(s) Of Engagement Activity | 2016 |
Description | ASM Conference on Biofilm 2015 - Talk Filloux |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Presentation of our work on the link between c-diGMP signalling and Biofilm formation or motility. Work currently submitted at PLoS Genetics. Title: Rewiring c-di-GMP signaling to control bacterial biofilm formation and motility, Place: Chicago (Il, USA), ASM Biofilm conference 0cober 24-29, 2015 |
Year(s) Of Engagement Activity | 2015 |
Description | Cyclic-di-GMP signalling regulating Pseudomonas aeruginosa lifestyles, 2014, EIMID meeting, Berlin, Valentini Poster |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Type Of Presentation | poster presentation |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Valentini M., Moscoso J., Hui K. and Filloux A., "Cyclic-di-GMP signalling regulating Pseudomonas aeruginosa lifestyles", EIMID meeting, 27 - 29 October 2014 Berlin, Germany. Poster stimulated discussion Well received and good feedback |
Year(s) Of Engagement Activity | 2014 |
Description | European Course on Microbial Evolution and Molecular Epidemiology, ENS Lyon |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Undergraduate students |
Results and Impact | Course for for French Microbiologists at the Ecole Normale Supérieure de Lyon |
Year(s) Of Engagement Activity | 2015 |
Description | FEMS2011- Geneva, Switzerland - Poster Moscoso |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Type Of Presentation | poster presentation |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | FEMS2011 (Geneva, Switzerland) - The role of cyclic di-GMP on the regulation of the T3SS/T6SS switch - Poster Moscoso Well received, excellent feedback |
Year(s) Of Engagement Activity | 2011 |
Description | FEMS2013- Leipzig, Germany- Poster Moscoso |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Type Of Presentation | poster presentation |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | FEMS2013- Leipzig, Germany- Poster Moscoso- The role of a protein, SadC, in biofilm formation Well received, excellent feedback |
Year(s) Of Engagement Activity | 2013 |
Description | Hidden Wonders: a journey into the bacterial world: Multimedia outreach -2014 |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Schools |
Results and Impact | 14-15/03/2014 - Hidden Wonders: a journey into the bacterial world: Multimedia outreach event presenting the key features of bacteria to members of the public, at Imperial College. School pupils were taught the importance of molecular studies in bacterial pathogens. We described the T6SS and c-di-GMP signalling in Pseudomonas aeruginosa in lay terms |
Year(s) Of Engagement Activity | 2014 |
Description | Imperial Festival - Super Bugs Zone - 27-29 April 2018 |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Schools |
Results and Impact | Open Day odf the CMBI at the annula Impetrial Festival. Several stands at the Supoer Bugs Zone describing multiple aspects of bacterial pathogens. |
Year(s) Of Engagement Activity | 2018 |
Description | Imperial Festival Outreach - Super Bugs Zone - Educating chirldren to the world of Microbiology |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Schools |
Results and Impact | Several members of the Filloux's group, Laura Nolan (PDRA), Patricia Bernal (PDRA), Lukke Allsopp (PDRA), Thomas Wood (PhD student), Panayiota Pissaridou (PhD student), Sophie Howard (PhD student) and Sara Planamente (PDRA), designed a stand in May 2017 for the Imperial festival. These were describing various aspects of Microbiology from recognizing a bacterium to detailed molecular mechanisms of an antibacterial weapon, the T6SS. |
Year(s) Of Engagement Activity | 2017 |
Description | Nanyang Technological University (NTU), Singapore Center for Environmental Life Sciences Engineering (SCELSE). |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | talk in the context of Networking with future collaborators in Singapore on biofilm, c-di-GMP signalling and T6SS |
Year(s) Of Engagement Activity | 2016 |
Description | National Chung-Hsing University, Taiwan |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Networking with our collaborator Erh-Min Lai in Taiwan |
Year(s) Of Engagement Activity | 2016,2017 |
Description | Presentation Ronan McCarthy International Symposium on c-di-GMP Signaling in Bacteria, Berlin, Germany.22-25th March 2015. |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | First meeting of its kind specifically focused on bacterial second messenger signalling. Ronan McCarthy resented a poster entitled "The Diguanylate Cyclase SadC Is a Central Player in Gac/Rsm-Mediated Biofilm Formation in Pseudomonas aeruginosa". |
Year(s) Of Engagement Activity | 2015 |
Description | Presentations Luke Allsopp and Ronan McCarthy at Antimicrobial Research Collaborative (ARC) launch, Imperial College, London, United Kingdom, September 25th, 2015 |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | Presentation by Luke Allsopp entitled; New insights into the T6SS of Pseudomonas. Presentation by Ronan McCarthy entitled; The Diguanylate Cyclase SadC Is a Central Player in Gac/Rsm-Mediated Biofilm Formation in Pseudomonas aeruginosa This was at the occasion of the kick off meeting of a new network of researchers from across the College and Imperial Healthcare Trust with an interest in the global challenge of AMR. |
Year(s) Of Engagement Activity | 2015 |
URL | http://www.imperial.ac.uk/arc |
Description | Summer School, Bacterial Biofilms : Biological, Mathematical and Physical perspectives, Nice, 2014 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Several aspects of bacterial biofilm formation and regulation have been discussed through different perspectives. I did communicate on the biology of biofilm and particularly on the regulatory mechanisms including c-di-GMP signalling After the talk the school asked the presentation being accessible on their website |
Year(s) Of Engagement Activity | 2014 |
Description | SuperBug Zone, Imperial Festival, Imperial College London |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Schools |
Results and Impact | On May 9-10, schools visited the MRC-CMBI and we had a super bug zone prepared for them. Ronan MacCarthy (PDRA BBSRC grant) was the Social Media Coordinator. He co-ran the social media profiles of the Superbug Zone over the two day public outreach event communicating Bacteriology and what are Microbes to the public (young and old) and also responding to questions online. Luke Allsopp (Marie Curie Fellow) co-ran the first stand on 'What are Bacteria'. Imperial Festival showcases to the public, alumni, staff and students all that Imperial has to offer and contributes. Also counts at the open day for the MRC CMBI. |
Year(s) Of Engagement Activity | 2015 |
Description | The International Graduate school-Molecular interactions of pathogens with biotic and abiotic surfaces |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Undergraduate students |
Results and Impact | Summer School for Microbiologists on Biofilm |
Year(s) Of Engagement Activity | 2014 |
Description | Twincore symposium, Hanover, Germany, 2013, Talk Filloux |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Participants in your research and patient groups |
Results and Impact | 5th Twincore symposium, Hanover, Germany, September 5-6, 2013, Pseudomonas aeruginosa lifestyles and infection strategy : a signalling story. I have discussed the implication of regulatory networks in the control Pseudomonas infection. Twincore is engaged in translational Medical Science. As part of the Scientific advisory board it also allows my laboratory to further establish connection in the field of applied medical research Well-received, excellent feedback |
Year(s) Of Engagement Activity | 2014 |