ENGINEERING SYNTHETIC SYMBIOSES BETWEEN PLANTS AND BACTERIA TO DELIVER NITROGEN TO CROPS

Lead Research Organisation: University of Oxford
Department Name: Plant Sciences

Abstract

Nitrogen is an essential element of biological molecules and life on earth. Lack of usable
nitrogen limits growth of microbes, plants, and animals. Lack of nitrogen in agricultural
soils limits plant production of food, feed, fiber and fuel. Nature solved the nitrogen limitation
problem via evolution of biological nitrogen fixation in diazotrophic bacteria that reduce
atmospheric N2 to NH3, which is readily assimilated into biological molecules. Biological
nitrogen fixation is catalyzed by a complex metalloenzyme called nitrogenase whose oxygen-sensitivity
may explain its restricted distribution amongst prokaryotes. Some plants, including most
legumes and a few non-legumes form intimate, nitrogen-fixing symbioses with diazotrophs that
provide the plants with ammonia. As a consequence, legumes have been an integral part of
sustainable agricultural systems for thousands of years. Unfortunately, many important food
species, including the grasses maize/corn, rice, and wheat cannot establish effective nitrogen-fixing
symbioses with diazotrophs, which means that they are dependent on nitrogenous fertilizers
for high yield. Large-scale use of industrially-produced N-fertilizer has doubled the influx
of N into the terrestrial biogeochemical N-cycle, with serious negative consequences for human
health and the natural environment. Therefore, the long-term sustainability of massive N-fertilizer
inputs in agriculture has come into question.
A team of six investigators has come together to solve the dual nitrogen problems of N-fertilizer
over-use in developed countries and soil N-paucity in developing countries by developing effective
endophytic and associative nitrogen-fixing symbioses in a model and a crop plant species.
The team brings together expertise in bacterial and plant genetics, genomics, biochemistry,
molecular and cell biology, physiology and synthetic biology with a deep knowledge of biological
nitrogen fixation. The overarching goal of the project proposed here is to develop effective
N2-fixing symbioses between the model C4-grass, Setaria viridis, or the related crop species,
Zea mays, and the endophytic bacterium, Rhizobium sp. IRBG74, or the associative bacterium,
Pseudomonas fluorescenes Pf5. Successful deployment of biological nitrogen fixation in model
or crop grass species will pave the way for a second Green Revolution that will increase crop
yields for resource-poor farmers and decrease the use and environmental-impact of industrial
N-fertilizers by wealthier farmers.

Technical Summary

We propose to develop a novel model system using the C4-grass Setaria viridis (Setaria), and its interaction with both a model endophyte (Rhizobium sp. IRBG74) and an associative bacterium, (Pseudomonas fluorescens Pf-5). Synthetic biology will be used to genetically alter Setaria and the two bacteria to ensure a lock and key interaction between plant and microbe, while maximizing nitrogen fixation by the bacteria and delivery of ammonium to the plant. A genetic system will be introduced into the bacteria that responds to environmental signals, a plant metabolite, and nutritional needs. These sensors will be integrated by synthetic circuits and used to control the expression of nod genes and the production of ammonia via a heterologous refactored nitrogen fixation pathway. Using a refactored system will enable the induction of this pathway in response to desired signals and will eliminate unwanted regulation, for example by oxygen and ammonia. This will be accomplished by designing assemblies of nif genes based on the nif gene clusters of model free-living nitrogen fixers exploiting the most robust nitrogen-fixing metabolism that allows oxygen sensitive nitrogenase to function during aerobic respiration.
A dual approach using an endophyte and an associative bacterium is proposed because the challenges to optimise nitrogen fixation faced by endophytes and associative bacteria are similar. Both approaches are essential because whether a nitrogen-fixing endophyte or an associative bacterium is ultimately deployed in the field will depend on different circumstances. Finally, while we will develop a model system based around Setaria in parallel we will genetically manipulate the ancient and important crop species, Zea mays (maize). This will allow us to advance from proof-of-concept to practical application in a crop species that is central to agriculture in both the developing and developed world.

Planned Impact

Nitrogen is one of the main constraints on agricultural productivity so its use is essential for high crop yields. In a world where food security is now considered a national priority crop yield is of critical importance. However, the drive for yield alone has led to very high application of nitrogen with consequent nitrate contamination of groundwater and problems of eutrophication. The problem is so serious that reactive nitrogen in the biosphere has doubled from preindustrial levels primarily through massive inputs into agriculture. It also results in the production of N2O which is around 300 times more potent than CO2 as a greenhouse gas. These are problems of regional, national and international scope that require urgent amelioration and are at the forefront of the grand challenges for UK science. By improving our understanding of how rhizobia develop into N2 fixing bacteroids in legume nodule we acquire the understanding to improve the competitive success of desirable strains of Rhizobium. It also lays down a foundation of understanding for the transfer of bacteria to nodules in other plants such as cereals. These aims are long term but ultimately this work has relevance to farming practice as well as government policy in decisions about nitrogen utilization in agriculture. It is also relevant to UK attempts to reduce greenhouse emissions and produce a low carbon economy. Understanding the nitrogen fixation and its role in the nitrogen cycle in agricultural has wider benefits applicable to the UK public because of its importance in food security and meeting international obligations for mitigating the effects of climate change. We propose to reach a wide audience of farmers, the public, national and international policy makers and charitable institutions through active outreach (Friends of John Innes). We also have strong links with "The Nitrous Oxide Focus Group" and the newly formed "Consortium for Legumes in Agriculture, Society and Environment", which is an international consortium to promote understanding on the use of legumes. In addition we have broad links to the environmenral impact of this work through the Earth and Life Systems Alliance between JIC and UEA (ELSA) and to the UK government via the "Living With Environmental Change program (LWEC)" which has its secretariat at UEA.

Publications

10 25 50
 
Description The aim of the whole project is to engineer a close relationship with nitrogen-fixing bacteria and specific (crop) plants, so that the plants benefit from the nitrogen fixed thus removing the need for financially costly and environmentally catastrophic levels of fertilizer. Using synthetic biology to modify both partners (plant and microbe) we have engineered a lock-and-key mechanism of interaction. To date we have achieved synthesis of a specific chemical in plants which can be metabolized only by certain bacteria. Increasing the specificity and efficiency of each of the steps in the process, through engineering of genetic switches and refactoring of genes to remove undesired levels of control is now the priority.
Exploitation Route The results of this project could open up the whole area of nitrogen fixation by non-leguminous crop plants. Improvements in the delivery of nitrogen to crops is hugely important, especially in parts of Africa and India where the soil is poor and nitrogenous fertilizer out of reach for many farmers. Reduction in use of nitrogenous fertilisers will have a positive impact on the environment.
Sectors Agriculture, Food and Drink,Environment

 
Description India-UK Nitrogen Fixation Centre (IUNFC)
Amount £1,680,948 (GBP)
Funding ID BB/N013387/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2016 
End 12/2018
 
Description Understanding and Exploiting Biological Nitrogen Fixation for Improvement of Brazilian Agriculture (Co-I with Prof. Ray Dixon as Lead PI)
Amount £1,624,659 (GBP)
Funding ID BB/N013476/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 02/2016 
End 01/2019
 
Description Understanding and Exploiting Biological nItrogen Fixation for Improvement of Brazilian Agriculture
Amount £1,624,659 (GBP)
Funding ID BB/N013476/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 02/2016 
End 01/2019
 
Description Brazil and nitrogen fixation 
Organisation Aberystwyth University
Department Institute of Biological, Environmental and Rural Sciences (IBERS)
Country United Kingdom 
Sector Academic/University 
PI Contribution Our emphasis is to study the physiology of bacterial growth and survival in the rhizosphere and how they establish symbiotic interactions with plants. A further focus of our work is the physiology and biochemistry of nitrogen fixation in legume nodules. Most recently we have been studying how bacteria attach to and colonise roots and have developed methods to open up the whole area of how plants control the microbial root microbiome.
Collaborator Contribution Our major emphasis is on signal transduction cascades that regulate nitrogen fixation genes in response to oxygen, carbon and fixed nitrogen status. A long-term goal of this research is to exploit and engineer nitrogen fixation genes for agricultural benefit. (JIC) Research in the Farrar lab focuses on increasing Miscanthus yields in order to replace fossil fuel usage, sequester atmospheric carbon, and ultimately contribute to climate change mitigation. In order to achieve this, there are two main research areas: Plant developmental biology & genetics and Plant-soil-microbe interactions. (IBERS) Plant electron microscopy: the ultrastructure of beneficial plant-bacterial interactions. I also specialise in immunolocalisation of both conventionally (chemically)- and cryo-fixed plant material. Expertise in nitrogen fixation by legumes and non-legumes (JHI)
Impact Grant awarded from BBSRC Newton Fund. Grant Ref: BB/N013476/1 Understanding and Exploiting Biological Nitrogen Fixation for Improvement of Brazilian Agriculture. PI: Prof. Ray Dixon
Start Year 2014
 
Description Brazil and nitrogen fixation 
Organisation James Hutton Institute
Country United Kingdom 
Sector Public 
PI Contribution Our emphasis is to study the physiology of bacterial growth and survival in the rhizosphere and how they establish symbiotic interactions with plants. A further focus of our work is the physiology and biochemistry of nitrogen fixation in legume nodules. Most recently we have been studying how bacteria attach to and colonise roots and have developed methods to open up the whole area of how plants control the microbial root microbiome.
Collaborator Contribution Our major emphasis is on signal transduction cascades that regulate nitrogen fixation genes in response to oxygen, carbon and fixed nitrogen status. A long-term goal of this research is to exploit and engineer nitrogen fixation genes for agricultural benefit. (JIC) Research in the Farrar lab focuses on increasing Miscanthus yields in order to replace fossil fuel usage, sequester atmospheric carbon, and ultimately contribute to climate change mitigation. In order to achieve this, there are two main research areas: Plant developmental biology & genetics and Plant-soil-microbe interactions. (IBERS) Plant electron microscopy: the ultrastructure of beneficial plant-bacterial interactions. I also specialise in immunolocalisation of both conventionally (chemically)- and cryo-fixed plant material. Expertise in nitrogen fixation by legumes and non-legumes (JHI)
Impact Grant awarded from BBSRC Newton Fund. Grant Ref: BB/N013476/1 Understanding and Exploiting Biological Nitrogen Fixation for Improvement of Brazilian Agriculture. PI: Prof. Ray Dixon
Start Year 2014
 
Description Brazil and nitrogen fixation 
Organisation John Innes Centre
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution Our emphasis is to study the physiology of bacterial growth and survival in the rhizosphere and how they establish symbiotic interactions with plants. A further focus of our work is the physiology and biochemistry of nitrogen fixation in legume nodules. Most recently we have been studying how bacteria attach to and colonise roots and have developed methods to open up the whole area of how plants control the microbial root microbiome.
Collaborator Contribution Our major emphasis is on signal transduction cascades that regulate nitrogen fixation genes in response to oxygen, carbon and fixed nitrogen status. A long-term goal of this research is to exploit and engineer nitrogen fixation genes for agricultural benefit. (JIC) Research in the Farrar lab focuses on increasing Miscanthus yields in order to replace fossil fuel usage, sequester atmospheric carbon, and ultimately contribute to climate change mitigation. In order to achieve this, there are two main research areas: Plant developmental biology & genetics and Plant-soil-microbe interactions. (IBERS) Plant electron microscopy: the ultrastructure of beneficial plant-bacterial interactions. I also specialise in immunolocalisation of both conventionally (chemically)- and cryo-fixed plant material. Expertise in nitrogen fixation by legumes and non-legumes (JHI)
Impact Grant awarded from BBSRC Newton Fund. Grant Ref: BB/N013476/1 Understanding and Exploiting Biological Nitrogen Fixation for Improvement of Brazilian Agriculture. PI: Prof. Ray Dixon
Start Year 2014
 
Description India and nitrogen fixation 
Organisation Indian Council of Agricultural Research
Department ICAR - Indian Agricultural Research Institute, New Delhi
Country India 
Sector Private 
PI Contribution I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation.
Collaborator Contribution University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system.
Impact A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised.
Start Year 2015
 
Description India and nitrogen fixation 
Organisation Indian Council of Agricultural Research
Department ICAR-Indian Institute of Soil Science
Country India 
Sector Learned Society 
PI Contribution I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation.
Collaborator Contribution University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system.
Impact A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised.
Start Year 2015
 
Description India and nitrogen fixation 
Organisation James Hutton Institute
Country United Kingdom 
Sector Public 
PI Contribution I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation.
Collaborator Contribution University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system.
Impact A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised.
Start Year 2015
 
Description India and nitrogen fixation 
Organisation John Innes Centre
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation.
Collaborator Contribution University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system.
Impact A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised.
Start Year 2015
 
Description India and nitrogen fixation 
Organisation Maharaja Sayajirao University of Baroda
Department Department of Bio-Chemistry
Country India 
Sector Academic/University 
PI Contribution I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation.
Collaborator Contribution University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system.
Impact A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised.
Start Year 2015
 
Description India and nitrogen fixation 
Organisation Oxford India Centre for Sustainable Development (OICSD)
Country United Kingdom 
Sector Public 
PI Contribution I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation.
Collaborator Contribution University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system.
Impact A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised.
Start Year 2015
 
Description India and nitrogen fixation 
Organisation The Energy and Resources Institute
Country India 
Sector Charity/Non Profit 
PI Contribution I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation.
Collaborator Contribution University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system.
Impact A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised.
Start Year 2015
 
Description India and nitrogen fixation 
Organisation University of Calcutta
Department Department of BioChemistry
Country India 
Sector Academic/University 
PI Contribution I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation.
Collaborator Contribution University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system.
Impact A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised.
Start Year 2015
 
Description India and nitrogen fixation 
Organisation University of Hyderabad
Department Department of Plant Sciences
Country India 
Sector Academic/University 
PI Contribution I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation.
Collaborator Contribution University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system.
Impact A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised.
Start Year 2015
 
Description ComMet conference, Microbiomes and Metagenomics, London, March 2015 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact The presentation stimulated the audience to ask broad-reaching questions.

Stimulated discussion amongst academics researching this topic.
Year(s) Of Engagement Activity 2015
URL http://rhizosphere.org/lab-news/
 
Description Plant Sciences Masterclass, Nick Crang and Josh Roworth 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Plant Sciences Masterclass, Nick and Josh were helping talented young biologists, who often assume that the best way to make a difference is to become a doctor. The out of hours masterclass series at the Botanic Garden aims to encourage A level biologists to think about plant science as an important and interesting area of biology and one that would allow them to tackle global changes and apply for courses that have a significant amount of plant science.
Year(s) Of Engagement Activity 2016
URL https://rhizosphere.org/lab-news/
 
Description 1st Plant Microbiome Symposium, Brazil Feb 2016 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact 1st Plant Microbiome Symposium, Brazil attended by approx. 200 people
Year(s) Of Engagement Activity 2016
 
Description Attending UBNFC Annual Meeting in Brazil, Nov 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Study participants or study members
Results and Impact Meeting of all collaborators on an international project or annual meeting
Year(s) Of Engagement Activity 2017
URL https://rhizosphere.org/lab-news/
 
Description BBSRC Senior Executive visits Plant Sciences Dept. Nov 2014 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Supporters
Results and Impact BBSRC visited the lab and heard short presentations from members of the group.

Interest was shown by the visitors in the work that was going on.
Year(s) Of Engagement Activity 2014
URL http://synthsym.org/news/
 
Description Back2Roots Advisor, annual project meeting. Jan 2017 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact International advisor to the Back2Roots research programme in the Netherlands
Year(s) Of Engagement Activity 2016
 
Description Bath Science Café presentation, June 2015 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Discussion followed presentation.

Increased requests for delivering talks at similar science cafe events in other geographical areas.
Year(s) Of Engagement Activity 2015
URL http://rhizosphere.org/lab-news/
 
Description Bayer Crop Science 16th-17th Feb 2016 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Bayer Crop Science 16th-17th Feb 2016. A group of academics advised industry
Year(s) Of Engagement Activity 2016
 
Description Botanic Gardens seminar 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact I talked to the Botanic Gardens about nitrogen and humanity

Lots of questions and feedback. This is widely advertised to the public
Year(s) Of Engagement Activity 2014
 
Description Bring and share pulse lunch 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Bring and share pulse lunch to celebrate world pulse day 18th Jan 2017
Year(s) Of Engagement Activity 2017
URL https://rhizosphere.org/lab-news/
 
Description ChemBioPlants Network 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact Workshop with approx. 100 researchers from three departments at University of Oxford
Year(s) Of Engagement Activity 2016
 
Description Cheney School, Oxford Ancient and Modern Science Festival, Kyle Grant 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Cheney School, Oxford Ancient and Modern Science Festival, talk by Kyle Grant
Year(s) Of Engagement Activity 2016
URL http://www.cheney.oxon.sch.uk/Calendar
 
Description ENFC, Budapest posters and talks 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact An international conference. Group members presented posters and gave talks to approx. 500 scientists
Year(s) Of Engagement Activity 2016
URL https://rhizosphere.org/lab-news/
 
Description Facebook LIVE video by Oxford Sparks 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Oxford Sparks FacebookLIVE podcast on nitrogen and the importance of nitrogen-fixing legumes
Year(s) Of Engagement Activity 2016
URL https://www.facebook.com/OxSparks/videos/1210777428999882/
 
Description FacebookLIVE - the future of food 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Schools
Results and Impact Facebook LIVE podcast in association with Oxford Sparks (latest 6.6k views)
Year(s) Of Engagement Activity 2017
URL https://www.facebook.com/OxSparks/videos/1210777428999882/
 
Description Fascination of Plants Day, University of Oxford, Harcourt Arboretum, May 2014; 2015; 2016 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Interest shown by general public in our displays and conversations concerning the importance of legumes.

Praise from public and interest in plants rose.
Year(s) Of Engagement Activity 2014,2015,2016
URL http://rhizosphere.org/lab-news/
 
Description Give peas a chance 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact We made a film with Oxford sparks on nitrogen fixation

Lots os hits on the web site
Year(s) Of Engagement Activity 2014
 
Description Global Pulse Day lunch, 18th Jan 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact 'Bring and share' lunch to publicise the use of pulses in cooking, their environmental importance and aspects of food security
Year(s) Of Engagement Activity 2016
URL http://pulses.org/global-pulse-day
 
Description Graduate School Speaker, Lunteren, The Netherlands, April 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Graduate School Speaker, Lunteren, The Netherlands, April 2017
Year(s) Of Engagement Activity 2017
 
Description International Conference on Nitrogen Fixation (ICNF) Asilomar, USA, Oct 2015 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Talk led to discussions. Poster sparked interest amongst audience.

Interest and approval of work from respected colleagues. Discussion of new ideas in the field.
Year(s) Of Engagement Activity 2015
URL http://rhizosphere.org/lab-news/
 
Description Martian Crops, publication in BANG! by Kyle Grant 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Undergraduate students
Results and Impact Magazine article on growing crops on Mars
Year(s) Of Engagement Activity 2016
URL https://rhizosphere.org/lab-news/
 
Description Martian Crops, talk in Oxford Town Hall, Science Week by Kyle Grant 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Talk as part of Oxford Science week on Martian crops, talk in Oxford Town Hall by Kyle Grant
Year(s) Of Engagement Activity 2016
URL https://rhizosphere.org/lab-news/
 
Description Meeting minds Oxford Alumni 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact I gave a talk to the Oxford biological sciences Alumni

Enthusiastic response from the audience
Year(s) Of Engagement Activity 2014
 
Description Michigan State University, student workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Student workshop at an American University where I was an international expert.
Year(s) Of Engagement Activity 2016
 
Description Microbiome of Agricultural Plants, Saskatchewan, Canada, June 2015 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Talk stimulated discussion.

Audience appreciation. Increased collaboration with other scientists.
Year(s) Of Engagement Activity 2015
URL http://rhizosphere.org/lab-news/
 
Description NUCLEUS UBNFC (VJC meeting) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact A joint meeting of two VJCs who are working in UK and Brazil, held in Brazil, Nov 2017
Year(s) Of Engagement Activity 2017
URL https://rhizosphere.org/lab-news/
 
Description Organised Nitrogen Network Workshop, June 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Nitrogen Network wa a one-day meeting for researchers interested in common themes
Year(s) Of Engagement Activity 2017
URL https://rhizosphere.org/lab-news/
 
Description Organised OxBacNet meeting - Feb 2018 (Alison East) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact University of Oxford, Bacteriology Symposium and Network meeting. Held three times a year.
Year(s) Of Engagement Activity 2018
URL https://rhizosphere.org/oxbacnet/
 
Description Organised a day of presentations: Tackling the nitrogen crisis; what are the solutions? Oxford, Sept 2015 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Type Of Presentation workshop facilitator
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact We organised a one-day symposium with international experts in the area. Extensive discussions were held throughout the day and new collaborations forged.

New collaborations have been sparked by this day's events, in terms of both existing programmes of work and in the instigation of new proposals and plans for more integrated research.
Year(s) Of Engagement Activity 2015
URL http://rhizosphere.org/nitrogen-crisis-meeting-sept-2015/
 
Description Outreach at Somerville College, Oxford Feb 2015 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Geographic Reach Local
Primary Audience Schools
Results and Impact School students asked questions about the course. They were asked to think about nitrogen and its fixation by legumes.

Local schools were keen to participate next year.
Year(s) Of Engagement Activity 2015
URL http://rhizosphere.org/lab-news/
 
Description Posters at ICNF, Granada, Spain, Sept. 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact International conference. 11 posters from Lab. 2 short presentations
Year(s) Of Engagement Activity 2017
URL https://rhizosphere.org/lab-news/
 
Description Ratan Tata visits Poole Lab, June 2015 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Ratan Tata, the prominent Indian businessman, visited the Poole Lab as part of a visit to Oxford. He expressed interest in the work that we are doing.

Increased awareness of our research amongst businessmen.
Year(s) Of Engagement Activity 2015
URL http://rhizosphere.org/lab-news/
 
Description Research Seminar, Lisbon, March 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Research Seminar, Lisbon, March 2017 at II Semana Bioengenharia, Instuto Superior Tecnico, Lisbon, Portugal
Year(s) Of Engagement Activity 2017
URL https://rhizosphere.org/lab-news/
 
Description Rhizosphere4, Maastricht, the Netherlands, June 2015 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Four posters from the lab were the focus of much interest.

Scientific audience appreciated the ideas behind the work.
Year(s) Of Engagement Activity 2015
URL http://rhizosphere.org/lab-news/
 
Description School of Life Sciences, University of Warwick, UK. Seminar, 29th April 2016 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact School of Life Sciences, University of Warwick, UK. Seminar, 29th April 2016, addressed audience made up of academic colleagues and post-grad students
Year(s) Of Engagement Activity 2016
 
Description Schools one week Intensive course 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact Intensive one week course for A level students in practical plant and microbial science

Students all keen to apply for science based university courses
Year(s) Of Engagement Activity 2014
 
Description Science Cafe, Sherborne, Dorset 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact A science cafe held in a village hall for approx. 60 members of the general public. Many interested participants.
Year(s) Of Engagement Activity 2016
URL https://sites.google.com/site/sherbornesciencecafe/
 
Description Seminar at University of Wisconsin-Madison, Oct 2015 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach Local
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Talk led to discussion. Met with interested parties for discussions over the course of the day.

Improved understanding of the area by colleagues working in closely related fields.
Year(s) Of Engagement Activity 2015
URL http://rhizosphere.org/lab-news/
 
Description Seminar, University of Southampton, Feb 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Seminar, Crop Systems Engineering Seminar Series, University of Southampton, Feb 2017
Year(s) Of Engagement Activity 2017
 
Description Seminars in Frontiers on Genomics, Centro de Ciencias Genomicas (CCG) of the National Autonomous University of Mexico, UNAM (Universidad Nacional Autónoma de México), Mexico, Oct 2015 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Geographic Reach Local
Primary Audience Undergraduate students
Results and Impact I presented three seminars to a group of undergraduates from Mexico in Seminars in Frontiers on Genomics series, organised by Centro de Ciencias Genomicas (CCG) of the National Autonomous University of Mexico. The seminars are broadcast to participating institutions in Mexico. One-to-one discussions were had with the students on a wide-range of topics to stimulate their interest.

Enormous appreciation and interest from the post-grad and under-grad students that I interacted with. Discussions with academics at the National Autonomous University of Mexico.
Year(s) Of Engagement Activity 2015
URL http://rhizosphere.org/lab-news/
 
Description Twitter account Rhizosphere @PooleLabOxford 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Twitter account to publicise the work of the Poole Lab
Year(s) Of Engagement Activity 2017
URL https://twitter.com
 
Description University of Middlesex research seminar (Barney Geddes) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact University of Middlesex research seminar (Barney Geddes) Environmental Sciences Lecture Series
Year(s) Of Engagement Activity 2017
URL https://rhizosphere.org/lab-news/
 
Description VI Annual Meeting of the Plant Genetic and Biotechnology Network, Graduate School, Italy, June 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact VI Annual Meeting of the Plant Genetic and Biotechnology Network, Graduate School, Italy, June 2017
Year(s) Of Engagement Activity 2017
URL https://rhizosphere.org/lab-news/
 
Description Visit to Murdoch University, WA 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Industry/Business
Results and Impact Spent 1 month in Australia as Sir Walter Murdoch Adjunct Professor with Australian colleagues. Liaising, gave a seminar and got involved with their research activities.
Year(s) Of Engagement Activity 2016
 
Description Visit to Murdoch University, Western Australia as Sir Walter Murdoch Visiting Fellow, Dec 2014 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Visit to another laboratory for several weeks. Included giving two seminars and extended discussions with colleagues in the field.

Strong ties formed and fostered with the research groups in universities in Western Australia.
Year(s) Of Engagement Activity 2014
URL http://rhizosphere.org/lab-news/
 
Description Website for Synthetic Symbioses Project (SynthSym.org) 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Website dedicated to publicising and describing the work on the Synthetic Symbioses (SynSym) project.
Year(s) Of Engagement Activity 2014,2015,2016,2017
URL https://synthsym.org
 
Description Wow! How? Family Science Fair, March 2013, 2014, 2015 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Geographic Reach Local
Primary Audience Schools
Results and Impact Demonstrations stimulated discussion about simple science with children aged 5-10yrs and their parents/carers

Favourable comments from the audience on how the stand sparked their interest.
Year(s) Of Engagement Activity 2014,2015
URL http://rhizosphere.org/lab-news/
 
Description Wymondham academy school visit 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Talk to 6th form students to encourage them to consider science as a career

Students keen to apply for university places
Year(s) Of Engagement Activity 2014