Integrated enzyme discovery and pathway optimisation for bagase valorisation
Lead Research Organisation:
University of Manchester
Department Name: School of Medical Sciences
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Organisations
- University of Manchester (Lead Research Organisation)
- Natura Brasil (Collaboration)
- University College London (Collaboration)
- Bioethanol Science and Technology Centre (CTBE) (Collaboration)
- Universidade de São Paulo (Project Partner)
- Brazilian Center for Research in Energy and Materials (Project Partner)
Publications
Berepiki A
(2020)
Development of High-Performance Whole Cell Biosensors Aided by Statistical Modeling.
in ACS synthetic biology
Machado LF
(2016)
Development and substrate specificity screening of an in vivo biosensor for the detection of biomass derived aromatic chemical building blocks.
in Chemical communications (Cambridge, England)
Machado LFM
(2022)
Directed Evolution of Transcription Factor-Based Biosensors for Altered Effector Specificity.
in Methods in molecular biology (Clifton, N.J.)
Moraes EC
(2018)
Lignolytic-consortium omics analyses reveal novel genomes and pathways involved in lignin modification and valorization.
in Biotechnology for biofuels
Tramontina R
(2020)
Consolidated production of coniferol and other high-value aromatic alcohols directly from lignocellulosic biomass
in Green Chemistry
| Description | The activity within this pump-priming grant was primarily focused on building an international collaboration team (UK-Brazil), to that end a number of visits and meetings were held between the UK and Brazilian project partners. This activity led to a successful large grant application looking to take the proposed research forward. In addition researcher exchange (UK to Brazil) took place, we developed a biosensor for chemical building block derived from biomass waste (lignin bagasse) which has now been published. |
| Exploitation Route | The research performed is now published in an Open Access journal, and allows researchers more efficient ways to screen for enzymes utilised in bio-mass valorisation. |
| Sectors | Chemicals Manufacturing including Industrial Biotechology |
| URL | http://pubs.rsc.org/en/content/articlelanding/2016/cc/c6cc04559f#!divAbstract |
| Description | BBSRC-FAPESP joint call in advanced biofuels |
| Amount | £2,082,439 (GBP) |
| Funding ID | BB/P01738X/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2017 |
| End | 02/2022 |
| Description | Cellulosomes - a versatile enzymatic scaffold platform for bioconversion of lignocellulosic biomass into value-added fine chemicals |
| Amount | £50,000 (GBP) |
| Organisation | São Paulo Research Foundation (FAPESP) |
| Sector | Public |
| Country | Brazil |
| Start | 03/2019 |
| End | 04/2020 |
| Description | Engineering Novel Allosteric Transcription Factor Recognition: Applications in Bio-catalysis and Synthetic Biology |
| Amount | £80,000 (GBP) |
| Funding ID | 2110033 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2018 |
| End | 09/2022 |
| Description | Proof of Concept - CMBNet |
| Amount | £35,032 (GBP) |
| Funding ID | BIOCATNET PoC-012 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Department | Networks in Industrial Biotechnology and Bioenergy (NIBB) |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 06/2015 |
| End | 10/2015 |
| Description | Rutherford Fund Strategic Partner Grants |
| Amount | £149,500 (GBP) |
| Funding ID | RF-2018-41 |
| Organisation | Department for Business, Energy & Industrial Strategy |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2018 |
| End | 03/2019 |
| Description | Science Without Boarders |
| Amount | £120,000 (GBP) |
| Organisation | National Council for Scientific and Technological Development (CNPq) |
| Sector | Public |
| Country | Brazil |
| Start | 09/2015 |
| End | 08/2020 |
| Description | UK Catalysis Hub |
| Amount | £172,000 (GBP) |
| Funding ID | EP/M013219/1 |
| Organisation | Engineering and Physical Sciences Research Council (EPSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2018 |
| End | 04/2020 |
| Description | Collaboration with Brazil Flavour and Fragrance Company |
| Organisation | Natura Brasil |
| Country | France |
| Sector | Private |
| PI Contribution | This Flavour and Fragrance Company are collaborators on a 5 year UK-Brazil Academia-Industry collaboration |
| Collaborator Contribution | Guidance on target molecules and markets being developing through the research program |
| Impact | Multi-discplainry 5 year collaboration, too early in the project for outputs |
| Start Year | 2017 |
| Description | Collaboration with CTBE, Brazil |
| Organisation | Bioethanol Science and Technology Centre (CTBE) |
| Country | Brazil |
| Sector | Private |
| PI Contribution | During a Faculty-led Research Development visit to Brazil, we visited the Brazilian National Laboratory for Bioethanol (CTBE). Following this visit, myself and Fabio Squina (CTBE) have been in communication about applying ours interests in synthetic biology to facilitate enzyme discovery associated with lignin-biomass valorisation. We hosted visitors from the CTBE and other research institutes in Brazil at Industrial Biotechnology Bioenergy Meeting that I co-organised. This led to a successful funding application to the BBSRC/FAPESP (FAPPA) pump-priming call, to allow us to explore these preliminary ideas and facilitate further networking and research consortia building activities. Most recently we re-visited the CTBE to further develop collaborators links and build a research proposal. This has led to exchange of materials, a proposed exchange of personnel between my laboratory and the CTBE, and most recently to a large funding application to the BBSRC/FAPESP (>£2.5 UK). |
| Collaborator Contribution | During a Faculty-led Research Development visit to Brazil, we visited the Brazilian National Laboratory for Bioethanol (CTBE). Following this visit, myself and Fabio Squina (CTBE) have been in communication about applying ours interests in synthetic biology to facilitate enzyme discovery associated with lignin-biomass valorisation. We hosted visitors from the CTBE and other research institutes in Brazil at Industrial Biotechnology Bioenergy Meeting that I co-organised. This led to a successful funding application to the BBSRC/FAPESP (FAPPA) pump-priming call, to allow us to explore these preliminary ideas and facilitate further networking and research consortia building activities. Most recently we re-visited the CTBE to further develop collaborators links and build a research proposal. This has led to exchange of materials, a proposed exchange of personnel between my laboratory and the CTBE, and most recently to a large funding application to the BBSRC/FAPESP (>£2.5 UK). |
| Impact | Outcomes: A proposed funding application to the BBSRC/FAPESP (>£2.5 UK). Multi-discplianry collaboration: Synthetic Biology, Meta-transcriptomics, Enzyme discovery, biocatalysis, bioprocess development, life-cycle analysis. |
| Start Year | 2013 |
| Description | Collaboration with UCL |
| Organisation | University College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We have developed whole cell biocatalysts for the biotransformation of waste/low value biomass residues into high-value fragrance and flavour compounds |
| Collaborator Contribution | Scale up of the biotransformation process converting waste/low value biomass residues into high-value fragrance and flavour compounds |
| Impact | Pending, outputs in preparation |
| Start Year | 2016 |
| Description | BBSRC Enterprise Fellowships Networking Event |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | BBSRC Enterprise Fellowships Networking Event |
| Year(s) Of Engagement Activity | 2017 |
| Description | Croda Open Innovation Day |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | New project ideas discussed. |
| Year(s) Of Engagement Activity | 2017 |
| Description | EuroScience Open Forum (ESOF) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | EuroScience Open Forum (ESOF): +provide an open forum for debate on science and technology and research policies in Europe +strengthen the links between science and society +contribute to the creation of an integrated space for science and technology in Europe, linking research organisations and policies at national and EU levels; strive for a greater role of the EU in research and +influence science and technology policies. |
| Year(s) Of Engagement Activity | 2016 |
| URL | http://manchester2016.esof.eu/en/ |
| Description | FLS Community Open Day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | An outreach stand about biotechnology for the production of fragrance compounds and public opinion of synthetic biology |
| Year(s) Of Engagement Activity | 2016 |
| Description | Institute Open Day (Outreach) |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Synthetic Biology stand at MIB open-day attended by >200 A levels students |
| Year(s) Of Engagement Activity | 2014,2015,2016 |
| Description | Invited speaker at CTBE and UNICAMP |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Participants in your research and patient groups |
| Results and Impact | Talks sparked Q&A, and supported subsequent discussions around research proposal Further funding applications |
| Year(s) Of Engagement Activity | 2014 |
| Description | SMART-Map Industrial Dialogue on Synthetic Biology |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Study participants or study members |
| Results and Impact | Workshop on the responsible development of Synthetic Biology which forms part of the EU H2020 SMART-map project. |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://projectsmartmap.eu/ |
| Description | SynBioChem Workshop on Responsible Research and Innovation |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Study participants or study members |
| Results and Impact | Roundtable discussant at SynBioChem Workshop on Responsible Research and Innovation |
| Year(s) Of Engagement Activity | 2015 |
| Description | iGEM Giant Jamboree |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Synthetic Biology iGEM Giant Jamboree |
| Year(s) Of Engagement Activity | 2016 |
| URL | http://2016.igem.org/Giant_Jamboree |