US Partnering Award: Using bioinformatics to predict and understand virus-host interactions to improve ASFV vaccines, therapeutics and diagnostics.

Lead Research Organisation: The Pirbright Institute
Department Name: Vector-borne Viral Diseases

Abstract

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Publications

10 25 50
 
Description The codon usage in genes encoded by African swine fever virus has been compared with those encoded by different chromosomes in the pig genome and by the Ornithodoros tick by Prof Gary Olsen, University of Illinois. The aim was to try and identify virus genes that may have been acquired more recently from either host. The results showed that the codon usage of ASFV genes is distinct from that of the pig and tick but clusters more closely with some of the pig chromosomes. Some ASFV genes were outliers from the others possibly due a more recent acquisition but other factors may be involved (eg gene length). The scatter in codon usage for the tick genes may be due to limited quantity and quality of the gene sequences available. RNA has been prepared from an Ornithodoros cell line for analysis by sequencing to obtain a better data set.
To discuss progress meetings have been held between the Ps and Prof Olsen in June 2014 and between the PIs Dixon and Shisler in June 2015. Telephone conferences have been held in December 2015 and March 2016. Further visits of Prof Joanna Shisler and Dr Lauren Gates-Tanzer to the Pirbright Insttute took place in October 2017 and February 2018. Dr Gates-Tanzer completed a placement at the Pirbright Institute between January and March 2018. During these visits experiments were planned to identify novel African swine fever virus inhibitors of innate immunity including of interferon induction and NFkB transcription factor. Dr Gates-Taylor transferred technology and reagents to Pirbright during her placement Jan -March 2018. As a result of the collaboration 15 new ASFV inhibitors of innate immunity were identified.
Further work has been carried out to understand the mechanism by which the African swine fever virus proteins identified in the initial screen inhibit the induction of type I Interferon and the activation of NF-kappa B transcription factor. This work involved identifying the stage in the activation pathways that are inhibited by the ASFV proteins. This provides further data for publication and applications for future funding. Dr Linda Dixon and Samuel Connell from Pirbright visited University of Illinois in June 2018 to discuss results and plan future experiments.
Exploitation Route By publication of data in open access in peer-reviewed journals. The inhibitors of innate immunity identified will be targets for deletion from ASFV genomes to construct candidate live attenuated vaccines. These are urgently needed since ASF is spreading through Africa, Europe and China with devastating economic impacts.
Sectors Agriculture, Food and Drink

 
Description Science Director Global Alliance for African swine fever virus research
Geographic Reach Multiple continents/international 
Policy Influence Type Influenced training of practitioners or researchers
Impact I was Science Director of the Global Alliance for Research on African swine fever virus (GARA). This involved being Chair of the Scientific Organizing Committee for 2 International Meetings and preparing reports and GAP analysis to direct research.
URL https://www.ars.usda.gov/GARA/
 
Description Collaboration on Structural Biology of African swine fever virus apoptosis inhibitors 
Organisation La Trobe University
Country Australia 
Sector Academic/University 
PI Contribution My research team provided expertise on African swine fever virus and identified host proteins that interact with virus inhibitors of apoptosis
Collaborator Contribution My partners (Drs Marc Kvansakul and Hinds La Trobe, Melbourne) determined the X-ray crystal structure of the African swine fever apoptosis inhibitor A179L and its binding affinity with interacting BH3 domain containing proteins
Impact Publication in J Virology. Grant Applications to Australian Research Council. Structural Biology, Virology, Cell Biology
Start Year 2014
 
Description Collaboration with Prof Joanna Shisler, University of Illinois 
Organisation University of Illinois
Department Department of Microbiology
Country United States 
Sector Academic/University 
PI Contribution We collaborated to identify new African swine fever virus immune evasion genes. We provided reagents and expertise.
Collaborator Contribution Our collaborator provided expertise and reagents
Impact Outputs are a review article and results for future publication
Start Year 2014
 
Description Invited Keynote Speaker International Society of Veterinary Epidemiology and Ecology, China Mai Thailand 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Keynote talk on progress towards African swin efever virus vaccine development at ISVEE meeting Thailand
Year(s) Of Engagement Activity 2018
 
Description Scientific Chair Vaccine Session Poxvirus, Asfarvirus, Iridovirus International Workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I was chairperson of the scientific session on Vaccines at the International Poxvirus, Asfarvirus and Iridovirus Workshop held in Strasbourg France June 2016. I selected abstracts for oral and poster presentations and acted as chair during the session.
Year(s) Of Engagement Activity 2016
 
Description Seminar on African swine fever virus 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Presentation on African swine fever virus vaccine development to Yebio Bioengineering and China Animal Health and Epidemiology Centre, Qingdao, PR China
Year(s) Of Engagement Activity 2018
 
Description Seminar on African swine fever virus 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Seminar on African swine fever virus host interactions and vaccine development. Yangzhou University Veterinary Faculty PR China
Year(s) Of Engagement Activity 2018
 
Description Seminar on African swine fever virus 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Seminar at Beijing Institute of Animal Sciences, Chinese Academy of Agricultural Sciences 12 Zhongguanchan South Stree, Haifing District Beijing
Year(s) Of Engagement Activity 2018
 
Description Seminar on African swine fever virus virus host interaction and vaccine development 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Seminar Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences PR China
Year(s) Of Engagement Activity 2018
 
Description Seminar on African swine fever virus virus host interaction and vaccine development 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Seminar on African swine fever virus evasion of host defences and vaccine development Institute of Molecular and Cellular Biology, UCL London
Year(s) Of Engagement Activity 2018
 
Description Seminar on prospects for African swine fever virus vaccine development 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Seminar at Harbin Institute for Veterinary Research, PR China
Year(s) Of Engagement Activity 2018
 
Description Seminar on virus host interactions and prospects for African swine fever virus vaccine development 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Seminar on African swine fever virus host interactions and vaccine development
Year(s) Of Engagement Activity 2018