RNA FISH to determine bacterial RNA polymerase functionalities required for sigma factor specific escape from antibiotic action
Lead Research Organisation:
Imperial College London
Department Name: Life Sciences
Abstract
USA
People |
ORCID iD |
| Martin Buck (Principal Investigator) |
Publications
Bashir T
(2022)
Molecular Origins of Transcriptional Heterogeneity in Diazotrophic Klebsiella oxytoca.
in mSystems
Buck M
(2015)
In vitro and in vivo methodologies for studying the Sigma 54-dependent transcription.
in Methods in molecular biology (Clifton, N.J.)
Engl C
(2020)
The route to transcription initiation determines the mode of transcriptional bursting in E. coli.
in Nature communications
Lawton E
(2014)
Determination of the self-association residues within a homomeric and a heteromeric AAA+ enhancer binding protein.
in Journal of molecular biology
Schaefer J
(2015)
Genome wide interactions of wild-type and activator bypass forms of s54.
in Nucleic acids research
Yang Y
(2015)
TRANSCRIPTION. Structures of the RNA polymerase-s54 reveal new and conserved regulatory strategies.
in Science (New York, N.Y.)
Zhang N
(2015)
Mutations in RNA Polymerase Bridge Helix and Switch Regions Affect Active-Site Networks and Transcript-Assisted Hydrolysis.
in Journal of molecular biology
Zhang N
(2016)
Transcription Regulation and Membrane Stress Management in Enterobacterial Pathogens.
in Advances in experimental medicine and biology
Zhang N
(2014)
Subunit dynamics and nucleotide-dependent asymmetry of an AAA(+) transcription complex.
in Journal of molecular biology
| Description | That signalling leads to noise in stress gene expression |
| Exploitation Route | In grant applications and in actual bench work-via training afforded |
| Sectors | Agriculture, Food and Drink,Healthcare,Pharmaceuticals and Medical Biotechnology |
| Description | We have employed the methods deveoped across a range of projects, including for our work on nitroigen fixation and the physiology of bacterial biofilms, known for their drug resistance. |
| Sector | Chemicals,Manufacturing, including Industrial Biotechology |
| Impact Types | Societal |
| Description | MRC AMR initiative |
| Amount | £250,000 (GBP) |
| Organisation | Medical Research Council (MRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 10/2015 |
| End | 09/2017 |