Bilateral BBSRC-FAPESP Research Grant: Dissecting Essential Roles of Chicken Interferon Stimulated Genes in the Pathobiology of Poultry Viruses

Lead Research Organisation: Lancaster University
Department Name: Division of Biomedical and Life Sciences

Abstract

Poultry sectors of the UK and Brazil are contributing significantly to the cheapest and the most accessible source of meat protein. The UK poultry industry is contributing around £4 billion to the national economy whereas Brazil is the largest producer of poultry and poultry products, after United States and China. Despite this high production, infectious diseases are a major hurdle in achieving increasing demands of poultry products, around the globe. The total losses caused by infectious disease are enormous. For example, only Marek's disease virus (MDV), a cancer-causing virus of poultry, accounts for £2 billion losses worldwide. Massive vaccines application remained major disease-controlling tool against viruses. Despite widespread vaccinations against viruses (e.g. 22 billion vaccine doses for MDV only per year), the threat from poultry diseases is on rise and highlights the gaps in our fundamental understanding of poultry responses against virus natural infections or in immunization (vaccines). Such gaps has left us indebted on the molecular mechanism of chicken's involvement in influenza zoonoses and as a result quarantine and slaughtering of infected birds, as only choice, cost the poultry industry billions of dollars. Interferons, produced following viral infection of avian cells, stimulate the expression of hundreds of genes (more than 350 in humans) called interferon stimulated genes (ISGs). Exploitation of these ISGs could potentially be applied in favour of vaccine production, better vaccination protocols and controlling infections. In this proposal, we will initially identify the nature and magnitude of chicken ISGs (chISGs) and study their effects against five avian viruses causing infections in poultry (chicken, duck, turkey) ranging from mild to severe in terms of clinical manifestation. These viruses are of immense commercial and zoonotic importance not only for UK and Brazil but also throughout the world. Using broad-range profiling systems, we ll identify those chISGs in the chicken cells that inhibit or enhance virus infections. Once identified, the factors that enhance virus infection will be overexpressed in established chicken cell line and the ones that inhibit virus infection will be removed or silenced within chicken cells. This modified cell line will be used to increase the replication of viruses for both industrial vaccine production and laboratory scale virus isolation. Given the fact that the current global poultry vaccine market is high, even a ten-fold increase in virus yield, which is potentially achievable, would have significant impact on the cost for vaccine. Beside industrial relevance, this new proposal, to be carried out jointly between The Pirbright Institute, UK and the State University of Campinas, Brazil, is crucial to understand the mechanism by which viruses induce interferon system of the chicken, some of which will be applicable to other species such as duck or turkeys. Finally, the findings from the project will be very valuable in examining the ability of the chISGs to protect the chickens against viruses of both poultry importance and those that can switch from poultry to human.

Technical Summary

To initiate successful virus replication cycle, viruses have to breach a cascade of host cellular responses. Among these barriers, innate immune responses are most potent and independent to nature of pathogens. The objectives of this work are to define the interactions between chicken interferon stimulated genes (chISGs) and diverse poultry viruses that are of economic importance both in Brazil and UK. Our preliminary transcriptomic and expression analysis indicate that chISGs are the most prominent immune system genes expressed in Marek's disease virus (MDV) infected chickens, indicating that chISGs essentially mediate pathobiology of viruses. In this proposal, we will dissect the significance and breadths of chISGs against poultry viruses using large-scale, genome-wide and high throughput screen platforms. Specifically, we will measure the temporal expression of ISGs that synergistically or antagonistically regulate specific virus replication using a pre-established lentivirus-based ISG library. We will combine these data with results gain through shRNA mediated gene silencing to catalogue chISGs with broad-spectrum or virus specific actions. These data will be integrated within mechanistic studies to provide an incredible rich data on how viruses interact with host immune responses, essentially contributed by ISGs, and the ways viruses have adapted to circumvent these responses. These experimentations will be performed with avian influenza virus, avian paramyxovirus and MDV at The Pirbright Institute, UK and with avian metapneumoviruses and infectious bursal disease virus at the State University of Campinas, Brazil using homogeneous system in two laboratory settings. Finally, the gained information will be utilized in determining markers of enhanced viral resistance and modifying a previously characterized cell line (DF-1) to increase the growth kinetics of avian viruses which otherwise are not growing in cell culture or grow at lower titer.

Planned Impact

Several factors such as climate changes, decline in agriculture land use and deforestation are seriously threatening global food security. In this scenario, poultry industry continues to expand at a dramatic rate in many parts of the worlds including the UK and Brazil, providing an economical source of proteins compared to other sectors of livestock. The poultry production in the UK fulfills 90% of the national demands and contributes annually around £4 billion to the UK economy supporting 60,000 associated jobs. Similarly, Brazil is an emerging economy with a large and increasing poultry industry for both egg (31.5 billion dozens annually) and poultry meat production (13.058 million tons, annually), which places Brazil among the three largest producers worldwide, along with United States and China. However, one of the major challenges to sustainable poultry production is the growth in infectious diseases, particularly caused by avian viruses. Therefore, understanding the complex interplay in host-pathogen interactions is crucial not only to devise control strategies but also to ascertain the role of chicken in the emergence of zoonotic pathogens. One of the aims of this proposal will be the academic work necessary to underpin and facilitate a range of benefits for different stakeholders including poultry industry, vaccine companies and scientific communities. The discovery of the role of chicken interferon stimulated genes (chISGs) will provide new insights into the avian innate immunity, which can be utilized to counter viral pathogens of poultry. There have been significant advancements in developing broad-scale and genome-wide platforms to understand the pathobiology of several pathogens in mammals, however, such state-of-the-art techniques in avian species are currently lacking. Knowledge with respect to molecular bases of host responses to virus infections, as the current proposal aims to achieve, will benefit avian research community to map chISGs expressionagainst wide range of environmental stress that compromise the poultry production. Due to current importance of global poultry vaccine market (currently $1,128 million and is expected to rise to $2,040 million by 2018) and lack of model cell line for poultry viruses (e.g. for MDV), the ultimate aim of this proposal is to utilize the information gained for the modification of a previously characterized cell lines which would have superior properties for enhanced virus replication both for vaccine production and vector delivery. These findings would have significant impact on vaccine doses per cell-passage, reduce the cost of vaccine and subsequently create opportunities to engage vaccine industry in the project. Outreach activities will be conducted to efficiently disseminate the information directly to stakeholders. The proposed studies will provide opportunities for the professional developments of scientists, and staff employed on this project through cutting edge scientific methods and technological advances both at The Pirbright Institute, UK and the State University of Campinas, Brazil. We will engage with the general public and scientific communities through continuous updating the information on websites of the consortium and partner's institutions, presentation of results in scientific meetings, and provision of press releases to media outlets. Scientists employed during the project will also be encouraged to take part in public engagement. In conclusions, improved understanding of the impact of viral infections and how the host responses contribute to the viral pathobiology will build bases to be used for devising better control measures, vaccine design and to underpin the crucial role of chicken in emergence of zoonotic influenza viruses. Collectively, the gained information, as described in this project, will have positive knock-on benefit both socially and for the national economies.

Related Projects

Project Reference Relationship Related To Start End Award Value
BB/M008681/1 01/09/2015 01/03/2018 £383,644
BB/M008681/2 Transfer BB/M008681/1 02/03/2018 01/03/2019 £133,084
 
Description Identification of the molecular mechanisms of IFIT5 protein in chicken
Mechanisms of action of this protein
Roles of ISGs against avian viruses.
Exploitation Route Availability of ISGs library
Optimized methodologies
Data to explore further
Sectors Agriculture, Food and Drink,Healthcare

 
Description Bat flu can spread to humans: should we be worried?
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a national consultation
URL https://theconversation.com/bat-flu-can-spread-to-humans-should-we-be-worried-112325
 
Description Newton Fund, Institutional Links
Amount £230,000 (GBP)
Funding ID 172710323 
Organisation British Council 
Sector Charity/Non Profit
Country United Kingdom
Start 04/2015 
End 09/2017
 
Description Responsive Mode and Institutional Link
Amount £383,644 (GBP)
Funding ID BB/M008681/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2015 
End 10/2018
 
Description BBSRC-FAPESP Joint Pump-Priming Awards for AMR and Insect Pest Resistance in Livestock and Agriculture 
Organisation University of Sao Paulo
Department Department of Virology
Country Brazil 
Sector Academic/University 
PI Contribution The PI from UK was invited by the BBSRC to take part in Partnership Building Workshop for upcoming call entitled: BBSRC-FAPESP Joint Pump-Priming Awards for AMR and Insect Pest Resistance in Livestock and Agriculture. The PI Muhammad Munir gave a talk on our current progress with BBSRC-FAPESP project and highlighted areas of common interests and expertise that can be exploited for collaboration in the future.
Collaborator Contribution All partners from CAPES in Institutional Links project were invited to discuss and plan the project application in this BBSRC-FAPESP Joint Pump-Priming Awards for AMR and Insect Pest Resistance in Livestock and Agriculture call. This include contribution by not only people within current Institutional Links project but patterns from other institutions were invited and collaborated.
Impact Following measurable outcomes can be outlined: Progress of Institutional Links programme, establishment of a new collaboration to further the findings of Institutional Links to applied aspects, strengthening of the existing partnership with researchers from Sao Paulo, CAMPINAS, and Kent (UK).
Start Year 2017
 
Description Collaboration with researchers working on the bats and wild birds 
Organisation Public Health England
Country United Kingdom 
Sector Public 
PI Contribution During the organization of Workshop at the Surrey University, we have invited more than 10 world-leading researchers that have made seminal contributions in the field. Initiation of collaboration is expected from this Workshop to plan and address fundamental question on animal and human health perspectives.
Collaborator Contribution All partners contributed in deciding the contents and selection of the speakers.
Impact It is expected to initiate a good and high level collaboration in the field of virology and public health
Start Year 2016
 
Description Collaboration with researchers working on the bats and wild birds 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution During the organization of Workshop at the Surrey University, we have invited more than 10 world-leading researchers that have made seminal contributions in the field. Initiation of collaboration is expected from this Workshop to plan and address fundamental question on animal and human health perspectives.
Collaborator Contribution All partners contributed in deciding the contents and selection of the speakers.
Impact It is expected to initiate a good and high level collaboration in the field of virology and public health
Start Year 2016
 
Description Collaboration with researchers working on the bats and wild birds 
Organisation Zoological Society of London
Country United Kingdom 
Sector Learned Society 
PI Contribution During the organization of Workshop at the Surrey University, we have invited more than 10 world-leading researchers that have made seminal contributions in the field. Initiation of collaboration is expected from this Workshop to plan and address fundamental question on animal and human health perspectives.
Collaborator Contribution All partners contributed in deciding the contents and selection of the speakers.
Impact It is expected to initiate a good and high level collaboration in the field of virology and public health
Start Year 2016
 
Description Funding from Royal Society and British Council 
Organisation Veterinary Laboratories Agency
Country United Kingdom 
Sector Public 
PI Contribution Within this BBRSC grant, we have establish links that led to a grant from Royal Society Research Grants RG2014 R2 and one from British Council
Collaborator Contribution We have held a meeting to discuss, plan and execute the application strategy.
Impact We have generated mini-ISGs library and are investigating to interogate their roles in the pathobiology of bats viruses Within BC grant, we are applying NGS pipeline to further our understanding on the virus population in Brazilian wildbirds and bats.
Start Year 2015
 
Description Meeting to Assess the Workshop and Plan Head 
Organisation Animal and Plant Health Agency
Country United Kingdom 
Sector Public 
PI Contribution During this meeting, representative of all UK partners have contributed their feedback and we evaluated the Workshop held at Surrey University. We also streamlined the next year plans for hosting the Poultry Health Course and field visit in Brazil.
Collaborator Contribution All partners contributed significantly in the Workshop and ensured that Brazilian participants learnt and interacted actively throughout the workshop. All partners are motivated to organise the Poultry Health Course in 2017 with same expected outcomes.
Impact Birds and Bats are important species of animals that can spread zoonotic pathogens to general public. Understanding the way they can pass viruses to human would help to establish control measures to safeguard human.
Start Year 2016
 
Description Meeting to Assess the Workshop and Plan Head 
Organisation St George's University of London
Country United Kingdom 
Sector Academic/University 
PI Contribution During this meeting, representative of all UK partners have contributed their feedback and we evaluated the Workshop held at Surrey University. We also streamlined the next year plans for hosting the Poultry Health Course and field visit in Brazil.
Collaborator Contribution All partners contributed significantly in the Workshop and ensured that Brazilian participants learnt and interacted actively throughout the workshop. All partners are motivated to organise the Poultry Health Course in 2017 with same expected outcomes.
Impact Birds and Bats are important species of animals that can spread zoonotic pathogens to general public. Understanding the way they can pass viruses to human would help to establish control measures to safeguard human.
Start Year 2016
 
Description Meeting to Assess the Workshop and Plan Head 
Organisation The Pirbright Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution During this meeting, representative of all UK partners have contributed their feedback and we evaluated the Workshop held at Surrey University. We also streamlined the next year plans for hosting the Poultry Health Course and field visit in Brazil.
Collaborator Contribution All partners contributed significantly in the Workshop and ensured that Brazilian participants learnt and interacted actively throughout the workshop. All partners are motivated to organise the Poultry Health Course in 2017 with same expected outcomes.
Impact Birds and Bats are important species of animals that can spread zoonotic pathogens to general public. Understanding the way they can pass viruses to human would help to establish control measures to safeguard human.
Start Year 2016
 
Description Meeting to Assess the Workshop and Plan Head 
Organisation University of Surrey
Country United Kingdom 
Sector Academic/University 
PI Contribution During this meeting, representative of all UK partners have contributed their feedback and we evaluated the Workshop held at Surrey University. We also streamlined the next year plans for hosting the Poultry Health Course and field visit in Brazil.
Collaborator Contribution All partners contributed significantly in the Workshop and ensured that Brazilian participants learnt and interacted actively throughout the workshop. All partners are motivated to organise the Poultry Health Course in 2017 with same expected outcomes.
Impact Birds and Bats are important species of animals that can spread zoonotic pathogens to general public. Understanding the way they can pass viruses to human would help to establish control measures to safeguard human.
Start Year 2016
 
Description Poultry Health Course from 3-7th April 2017 
Organisation University of Sao Paulo
Department Department of Virology
Country Brazil 
Sector Academic/University 
PI Contribution Researchers, students and professionals shown below participated in the Poultry Health Course organised by The Pirbright Institute and British Partners of the Institutional Links Ana Caroline de Souza Barnabé Gabriela Mansano do Nascimento Matheus Cavalheiro Martini Michael Edward Miller Paulo Anselmo Nunes Felippe Raissa Marques Beck Renato Luís Luciano Raphael Mausbach Simao Izabelle Mariane Cordeiro Helena Lage Ferreira Laís Santos Rizotto Nelson Rodrigo da Silva Martins Carla Meneguin Barbosa Stella Rezende Melo Fernando Gomes Buchala
Collaborator Contribution All British and Brazilian collaborators interacted and participated to discuss diseases of poultry and the areas that need future research and collaboration.
Impact Following benefits can be extracted: 1. Overview of the current disease statuses in UK and Brazil 2. Crucial areas of common research 3. Training and networking on avian diseases 4. Possibility of future collaborations on these crucial points
Start Year 2017
 
Description This Brazil Partnering Award was used for developing research collaboration that led to the winning a BBSRC grant (BB/M008681/1) application under BBSRC FAPESP call.The links established through this award have also led to the grant from British Council (172710323). 
Organisation St George's University
Country Grenada 
Sector Academic/University 
PI Contribution Both the concept generation, background data, and drafting of the proposal.
Collaborator Contribution Facilitated in finding key issues in the birds and bats populations in Brazil. Partners from Brazil were funded to participate in the First Global Alliance for Research on Avian Diseases (GARAD) meeting in London in July 2015 attended by over 120 participants from around the world.
Impact This Brazil Partnering Award was used for developing research collaboration that led to the winning a BBSRC grant (BB/M008681/1) application under BBSRC FAPESP call. The links established through this award have also led to the grant from British Council (172710323). Partners from Brazil were funded to participate in the First Global Alliance for Research on Avian Diseases (GARAD) meeting in London in July 2015 attended by over 120 participants from around the world.
Start Year 2015
 
Description This Brazil Partnering Award was used for developing research collaboration that led to the winning a BBSRC grant (BB/M008681/1) application under BBSRC FAPESP call.The links established through this award have also led to the grant from British Council (172710323). 
Organisation University of Surrey
Country United Kingdom 
Sector Academic/University 
PI Contribution Both the concept generation, background data, and drafting of the proposal.
Collaborator Contribution Facilitated in finding key issues in the birds and bats populations in Brazil. Partners from Brazil were funded to participate in the First Global Alliance for Research on Avian Diseases (GARAD) meeting in London in July 2015 attended by over 120 participants from around the world.
Impact This Brazil Partnering Award was used for developing research collaboration that led to the winning a BBSRC grant (BB/M008681/1) application under BBSRC FAPESP call. The links established through this award have also led to the grant from British Council (172710323). Partners from Brazil were funded to participate in the First Global Alliance for Research on Avian Diseases (GARAD) meeting in London in July 2015 attended by over 120 participants from around the world.
Start Year 2015
 
Description This Brazil Partnering Award was used for developing research collaboration that led to the winning a BBSRC grant (BB/M008681/1) application under BBSRC FAPESP call.The links established through this award have also led to the grant from British Council (172710323). 
Organisation Veterinary Laboratories Agency
Country United Kingdom 
Sector Public 
PI Contribution Both the concept generation, background data, and drafting of the proposal.
Collaborator Contribution Facilitated in finding key issues in the birds and bats populations in Brazil. Partners from Brazil were funded to participate in the First Global Alliance for Research on Avian Diseases (GARAD) meeting in London in July 2015 attended by over 120 participants from around the world.
Impact This Brazil Partnering Award was used for developing research collaboration that led to the winning a BBSRC grant (BB/M008681/1) application under BBSRC FAPESP call. The links established through this award have also led to the grant from British Council (172710323). Partners from Brazil were funded to participate in the First Global Alliance for Research on Avian Diseases (GARAD) meeting in London in July 2015 attended by over 120 participants from around the world.
Start Year 2015
 
Description 2nd Meeting Institutional Link 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Introduction:
• After a brief introduction, Muhammad Munir briefly updated about the previous meeting and current standing. The funding released and activities conducted so far were briefly discussed.
• The major object of the meeting was to organize the course "Diagnosis and Surveillance of Avian and Bat Viruses".
• After a direct discussion between the partners, we agreed upon:

o Budget: £30,000 (all inclusive) (being transferred to APHA)
o Participants: 15 (all Brazilian) 2k/participant
o Duration: 1 week
o Venue: Surrey University
o Host: Tony, Dan and Alex
o Participants selection: Brazilian partners
o Criteria for selection: Diverse disciplines including field veterinarians, English language, age (early career and senior).
o Dates of the course: Week commencing from 27th June.
o Potential speakers were identified

Actions to take:

1. Course contents, programme, and Speakers (Tony, Dan, Alex, Muhammad)
2. Course organization (Tony, Dan, Alex, Muhammad, Andrew Breed)
3. Selection of course participants (Edison, Clarice and Helena)
4. October was agreed to be good time for visit of the British Partners to Brazil

Next Meeting

To further fine tune the planning of the course and to deal bits and pieces, a focused meeting will be organized and will be opened for all to participate.
Year(s) Of Engagement Activity 2016
 
Description 3rd Meeting Institutional Link 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Venue: APHA
Date: 15th March 2016
Time: 10:00 to 15:00
Attendees: Steve Goodbourn, Muhammad Munir, Tony Tooks, Dan Horton, Andrew Breed

Following tentative course contents were decided and agreed upon.

Mon 27th 0900-1000 Atrium (registration) 1000-1300 Lecture theatre and three small group rooms 13:00-17:00 Pathology laboratory, 1800-22:00 Atrium (wine reception/poster session)
Tues 28th 0900-17:00 Lecture theatre and three small group rooms 1300-1700 computer rooms in case we want to run an Epi/Surveillance exercise (this could be on the main campus)
Wed 29th 0900-1300 Lecture theatre and three small group rooms 1300-1700 Molecular teaching laboratory
Thurs 30th 0900-1300 Lecture theatre and three small group rooms 1300- 1700 computer rooms
Friday 1st 0900-1300 Lecture theatre and three small group rooms

I believe there were also actions for Andrew to contact Ruth Cromie about a potential social/field visit to WWT

One thing I noted from the minutes of the previous meeting, is that the funds will be transferred to APHA for this workshop- that is fine but I have been informed that VAT will get added to the costs if we have to invoice.
Year(s) Of Engagement Activity 2016
 
Description Avian Genetics and Immunity 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact We are gathering people working on avian genetics and immunity to share experiences, discuss future plans and to identify research gaps.
Year(s) Of Engagement Activity 2017
URL http://geneticsandimmunity.com
 
Description Institutional Links Meeting Friday 17th February 2017 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact We discussed that all Brazilian visitors will be furnished with all expenses except tuition fee/research fee. Thus research costs have to be borne by hosting institutions/researchers. Copy of vaccination history against Rabies would be required from these visitors. 2ml blood samples from visitors would be needed before visit. Filling of the forms would be needed by these visitors
Year(s) Of Engagement Activity 2016
 
Description Invited Talk at Rochester Medical University New York, USA: Antiviral Potential of Chicken IFIT5 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact During the talk, several questions were asked and possibilities for the future collaboration was assessed. Taking on from there, we are planning a joint USA-UK application in coming call from different councils.
Year(s) Of Engagement Activity 2017
 
Description Meeting of all British and Brazilian partners on 12 October to streamline the workplan 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach International
Primary Audience Participants in your research and patient groups
Results and Impact We aim to identify the role of each partners, out of seven in total, and will make a practical layout for execution.

This activity will prepare the partner to initiate the work proposed in the project and to take active role in their dedicated tasks.
Year(s) Of Engagement Activity 2015
 
Description Meeting with Brazilian Delegate in Guildford 3rd April 2017 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact 15 Participants from Brazil joined the team of British partners at Guildford Harbour Hotel. Delegate participated in the Poultry Health Course from 3rd to 7th April where lectures from national and international scientists were presented. We discussed the training scope within this collaboration and planned the mission in Brazil.
Year(s) Of Engagement Activity 2017
 
Description Participation in the Global Alliance for Research on Avian Diseases 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The award funds were used to participate in the First Global Alliance for Research on Avian Diseases (GARAD) meeting in London in July 2015 attended by over 120 participants from around the world.
Year(s) Of Engagement Activity 2015
URL http://garad.org/
 
Description Participation in the Global Alliance for Research on Avian Diseases 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact GARAD is a freshly launched forum, led by The Pirbright Institute, UK, to establish networking among avian researchers from all continents. Primary aim of this initiative is to share and exchange latest research advances, promote collaborations and to maximize the use of resources and expertise to progressively control of avian diseases, around the world. The second International Conference of the Global Alliance for Research on Avian Diseases (GARAD) was held on 17-19 January 2018, in Hanoi, Vietnam. The conference, attended by over 150 delegates from academia, poultry breeding/farming and the pharmaceutical industry, discussed the major challenges and research advances in control of poultry diseases. Amongst the topics were, the continued threat from avian influenza, antigenic shifts/drifts and the risks of disease transmission within and from live bird markets, the challenges from antigenic diversity of other avian viruses, innovative approaches for poultry vaccine development and the potential opportunities to introduce genetic resistance to infectious agents through novel gene editing approaches. In separate interactive sessions, delegates actively debated the challenges, priorities and opportunities for academia in driving avian disease research, importance of developing an improved disease measures by industry and the contribution by the farming sector in the low and middle-income countries.
Year(s) Of Engagement Activity 2018
URL http://garad.org/
 
Description Partners from University visited to The Pirbright Institute 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? Yes
Geographic Reach National
Primary Audience Participants in your research and patient groups
Results and Impact Decision on the holding workshop and possible expansion on student exchange between Brazilian and British institutions.

Planed the use facilities in the University of Surrey for the holding the poultry health course.
Year(s) Of Engagement Activity 2015
 
Description Presentation in GARAD-2018 in Vietnam: Potential of chicken IFIT5 in Establishing Antiviral State 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact In the Global Alliance for Research on Avian Diseases 2018, I have presented our work on current understanding of IFIT5 mediated antiviral potential in avian species.
Year(s) Of Engagement Activity 2018
URL http://www.theeventsportal.com/GARAD-2018/3/Home
 
Description The 15th Meeting of the Avian Immunology Research Group 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The AIRG 2018 programme will include both fundamental science and practical application of avian immunology. We welcome presentation of work relating to key commercial poultry species as well as work presented in the context of the comparative biology or evolution of immune responses in a broader context including those relating to wild birds. We are delighted to invite all stakeholders, including senior scientists and young researchers from academia, industry and government organisations, to join us in Oxford in the autumn of 2018. More than 150 participants representing 15 countries participated.
Year(s) Of Engagement Activity 2018
URL http://airg.zoo.ox.ac.uk/
 
Description UK-Philippines Workshop 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact The organizers headed by Dr. Muhammad Munir of Lancaster University and Dr. Dennis V. Umali of the University of the Philippines Los Baños (UPLB), successfully conducted the "Newton Agham Researcher Links Workshop: Novel Vaccines and Diagnostic Technologies Against Emerging and Re-Emerging Veterinary Pathogens" held from 4 to 7 February 2019 at the Rizal Park Hotel Manila, Philippines. In the opening ceremonies, Dr. Jovencio Hubert A. Abalos, Assistant to the Dean and representing the dean of the College of Veterinary Medicine (CVM), UPLB welcomed the participants and wished them a fruitful workshop. He also mentioned that the topics to be covered in the workshop could bring out new topics for research for the dual Doctor of Philosophy programme being planned between UK universities and UPLB. Undersecretary Carol M. Yorobe representing the Department of Science and Technology (DOST), Philippines said that she hoped that the joint discussions will result in a collaborative network among UK and Philippine scientists. Mr. Norly Villar, Programme Manager, Newton Agham, British Council of the Philippines, also graced the occasion and welcomed the participants as well as inspiring and challenging them for a 4-day workshop.

Dr. Muhammad Munir, Lecturer, Lancaster University and one of the organizers, presented the mechanics of the activities ahead. He stated the objectives and expected outcomes, which are identification of research gaps, establishment of collaborations, identify potential threats to livestock and poultry and production of a white paper to be presented to policy makers and funding bodies.

The workshop was conducted for four days. On the first day, there were 9 papers presented, divided into 3 sessions. Each paper was read for 30 to 40 minutes, then a 10-minute open forum followed after each session. On the second day, there were 6 papers in 2 sessions, followed by two interactive sessions (workshops). On the third day, there were 6 papers in 2 sessions, followed by an interactive session (field trip), which included a cultural dinner. On the fourth day, there were 4 papers in one session. In summary, there were 25 technical papers and 3 interactive sessions. In general, the papers and workshops had a common goal, that of controlling and preventing infectious diseases of farm animals. Most of the papers focused on molecular techniques for the production of diagnostic kits and vaccines.

The abstracts of all the papers were published as proceedings and distributed to all participants before the workshop while the highlights of each paper, workshop outputs of Interactive Sessions 1 and 2, and the powerpoint presentations are attached as Appendices A, B, C and D, respectively. In Interactive Session 3 (Networking and Outdoor Activities), the participants had a field trip at the Philippine Genome Center (PGC) situated at the University of the Philippines Diliman, Quezon City. Selected staff of the facility welcomed them warmly and immediately proceeded in conducting a guided tour. The group visited the Shared Genomics Core Laboratory (SGCL) of the PGC, which primarily aims to build the capacity of local researchers to perform top-of-the-line genomics research. Also visited was the DNA Sequencing and Bioinformatics Facility (DSBF) of the PGC, which offers training packages in Basic Molecular Biology, Sanger Sequencing, Next Generation Sequencing and Bioinformatics. The activity provided everyone a glimpse of the capability of the center as well as giving an opportunity for potential collaborations, networking and information exchanges. Part of the interactive session was the cultural dinner at the Barbara's Heritage Restaurant in historic Intramuros, Manila. The cultural show composed of songs and dances featured the rich Filipino culture while providing a venue for strengthening British-Filipino camaraderie among the participants.

In the Closing Ceremony, Mr. Norly Villar, Programme Manager of the Newton Agham, British Council of the Philippines, presented the current programs and research opportunities offered by the British Council. He reported that there are 21 ongoing scholars and the first batch is expected to return in 2019 or 2020. He encouraged everyone to visit the Newton Agham Programme website for more details. Dr. Synan S. Baguio, Supervising Science Research Specialist and Officer-in-Charge, Livestock Research Division, Philippine Council for Agriculture, Aquatic and Natural Resources Research Development (PCAARRD), DOST delivered an inspirational message. He thanked everyone for a successful workshop and hoped that the shared information would be put to practical use and continue the collaboration among the participants. Dr. Rio John T. Ducusin, a professor of the College of Veterinary Medicine, UPLB who served as rapporteur during the entire workshop, read the agreement (Appendix E), which is one of the outputs of the workshop. He mentioned that hopefully, in the not-so-distant future, the items listed in the above document would be realized. The agreement was duly signed by the participants. The closing remarks were delivered by Dr. Jovencio Hubert A. Abalos, Assistant to the Dean of the College of Veterinary Medicine, UPLB. He thanked the organizers, sponsors, participants and everyone who contributed to the success of the workshop. He wished everyone a safe travel going back home.
Year(s) Of Engagement Activity 2019
URL http://uk-philippines-workshop.com/
 
Description Visit of British Partners to Brazilian Collaborators Laboratories 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Three British Collaborators within Institutional Links project including Muhamamd Munir (The Pirbright Institute), Dan Horton (Surrey University), and Denise Marshal (Animal and Plant Health Laboratory Agency) visited University of Sao Paulo and discussed the project progress with Brazilian collaborators. In order to understand the situation of poultry and bats in Brazil, several outreach activities were performed: 1. A visit of the poultry farm and collection of samples for routine screening of viruses, 2. Visit of Quarantine Facility to assess the import and export regulation of live animals, 2. visit of the bats enriched areas to see the diversity of bats populations. As a result of this visit, an interest group was established to pursue funding from BBSRC new call between UK and Brazil: http://www.bbsrc.ac.uk/funding/filter/2017-bbsrc-fapesp-awards-amr-insect-pest-resistance-in-agriculture/
Year(s) Of Engagement Activity 2017
URL http://www.bbsrc.ac.uk/funding/filter/2017-bbsrc-fapesp-awards-amr-insect-pest-resistance-in-agricul...
 
Description Workshop on Epidemiology and Diagnosis of Viruses in Birds and Bats 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact We hold a workshop entitled "Epidemiology and Diagnosis of Viruses in Birds and Bats" at University of Surrey, Surrey, UK to host 21 Brazilian participants having interests in viruses of bats and birds. Among participants, there were students, professionals, decision makers from Brazil Education Ministry, professors and field practitioners. We hold interactive discussion sessions, expert presentations from British and Brazilian researchers, practical sessions and field trips. All participants from Brazil and 6 researchers from UK visited London WWT wetland and participated in the "bats walk". During this evening walk, participants listened bats and identified species of bats most common in the UK. Additionally, day time visit helped them to see the birds population and their possible transmission models in the sate WWT wetland. This interactive participation not only help to identify the current research trends in the field but also to assess the possible transmission of viruses from birds and bats.
Year(s) Of Engagement Activity 2016