14TSB_ATC_IR Control of Botrytis in fresh produce pre- and post-harvest environments by integrated UV-B, -C and ethylene removal treatment

Lead Research Organisation: University of Nottingham
Department Name: Sch of Biosciences

Abstract

The fungal plant pathogen, Botrytis cinerea, is estimated to cause losses of over £88 million per year to the tomato and cut flower industries in the UK, industries worth over £2.2 billion per year to the UK economy. This is in the form of reduced shelf life for fruits, reduced vase life for flowers, and the costs of current control methods by the growers, which are largely based on the use of fungicides. With the desire to reduce pesticide usage, the industry is working hard to secure zero residue production techniques. Ultraviolet light UVB and UVC treatments of plants pre and post-harvest are two such strategies with great potential. UVC works mainly preharvest by inducing resistance in plants against the fungus, whilst UVB works postharvest by removal of ethylene and retardation of plant senescence and fungal development. In this project we will be optimising regimes for UVB and UVC treatments of tomato and cut flowers pre and post harvest to obtain maximal disease control and maximal enhancement of post-harvest commercial properties of the produce (increased shelf-life/vase life). This will also include piloting automated methods for applications of the optimal doses in commercial settings. In addition, we will be using advanced molecular biology techniques to formulate a deeper understanding of the mechanisms through which the UVB and UVC treatments work to control the diseases. The resultant approaches are predicted to result in savings to the industry of up to £220 million over a five year period.

Technical Summary

The fungal plant pathogen, Botrytis cinerea, is estimated to cause losses of over £88 million per year to the tomato and cut flower industries in the UK, industries worth over £2.2 billion per year to the UK economy. This is in the form of reduced shelf life for fruits, reduced vase life for flowers, and the costs of current control methods by the growers, which are largely based on the use of fungicides. With the desire to reduce pesticide usage, the industry is working hard to secure zero residue production techniques. Ultraviolet light UVB and UVC treatments of plants pre and post-harvest are two such strategies with great potential. UVC works mainly preharvest by inducing resistance in plants against the fungus, whilst UVB works postharvest by removal of ethylene and retardation of plant senescence and fungal development. In this project we will be optimising regimes for UVB and UVC treatments of tomato and cut flowers pre and post harvest to obtain maximal disease control and maximal enhancement of post-harvest commercial properties of the produce (increased shelf-life/vase life). This will also include piloting automated methods for applications of the optimal doses in commercial settings. In addition, we will be using advanced molecular biology techniques to formulate a deeper understanding of the mechanisms through which the UVB and UVC treatments work to control the diseases. The resultant approaches are predicted to result in savings to the industry of up to £220 million over a five year period.

Planned Impact

The initial beneficiaries of this research will include UK tomato and cut flower retailers, British tomato growers and British UK and overseas cut flower producers. Through the involvement of these industries in this project, and through the activities of May Barn Consultancy as an interface between the science outputs and grower practice, the outcomes on efficacies of control regimes, combined with the feasibility of commercialisation of fully automated delivery systems will be delivered to commercial concerns and other relevant stakeholders. The potential commercial impact of reducing losses caused by botrytis has been estimated at up to £220 million over 5 years in the UK alone, with the added impact that the extended shelf-life of produce could allow producers to expand and consider export opportunities in appreciable quantities. The pathway to impact beyond the commercial partners is also likely to involve commercialisation of the automated UVC delivery system for glasshouse use. The aim of the project is to provide a blueprint/model for UVB and UVC delivery that will then be an opportunity for a commercial concern to produce and market the equipment as a commercial business for UK and overseas markets.

Publications

10 25 50
 
Description • Ethylene removal had no effect on Botrytis control but did control other diseases (for example, Colletotrichum gloeosporioides and Penicillium expansum). In low ethylene concentrations, disease occurred 2 days later and sporulation was reduced.

• Work on UV-C showed that the pulsed light treatment induced a broad stress response. This reaction was similar to the response to a plant fungal infection and pulsed light may act as a preparation for subsequent infection.

• Mechanisms identified included enhanced perception and response to pathogens, upregulated stress receptors, kinases, Ca2+ and reactive oxygen (ROS) signalling components, ethylene and jasmonic acid response genes.

• Cell wall reinforcement - lignin and secondary metabolite biosynthesis.

• Synthesis of defence proteins, such as chitinases, glucanases, PR1, protease inhibitors, osmotin, peroxidases.

• In the absence of disease, the plants are being prepared to resist infection by Botrytis, for example.

• ROS quenching, specifically glutathione and thioredoxin systems.
Exploitation Route The findings, including details of optimum treatment regimes are currently being compiled into a Growers guide by the consortium partners for distribution to the UK horticultural industry. The ethylene treatment systems are being taken forward by the project partner that markets this system, Nutricyle, for further uses in the horticultural industry based in part on the outcomes form this work, whilst the UV-C equipment partners, Lambda, are also exploring ways to market their equipment based on the findings.
Sectors Agriculture, Food and Drink

 
Description The industrial partners that were involved in this project are currently exploring potential markets for ethylene scrubber equipment, as a means of reducing postharvest losses in horticultural products and extending shelf life.
First Year Of Impact 2018
Sector Agriculture, Food and Drink
Impact Types Economic

 
Description End of Project Meeting 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact The meeting was with Industry representatives and Innovate UK / DBEIS representation to explain the results of the project and to explore the possibilities for further exploitation of the outcomes of the work with industry.
Year(s) Of Engagement Activity 2018