Acute phase protein as biomarkers of disease in livestock and aquaculture
Lead Research Organisation:
University of Glasgow
Department Name: College of Medical, Veterinary, Life Sci
Abstract
Acute phase proteins (APP) are proteins which increase in concentration in blood in response to diseases caused by infection and inflammation. Measuring level of one or more APP in blood provides valuable diagnostic information, especially in human medicine where these tests for APP have been available for several decades. In animals there are significant differences in the proteins involved and in the responses found. Our laboratory is a leading centre for research into APP in animals and has identified the best target protein to measured in each species. This project is designed to develop rapid, robust and economic assays to enable laboratory tests for APP to be available for use in cattle, chicken and salmon production to the benefit of animal health and welfare. Further test systems will be developed, which will allow a veterinarian to test for raised APP levels on a farm visit, assisting in rapid diagnosis and treatment.
Organisations
Publications
Braceland M
(2017)
Technical pre-analytical effects on the clinical biochemistry of Atlantic salmon (Salmo salar L.).
in Journal of fish diseases
Braceland M
(2017)
Selective precipitation reaction: a novel diagnostic test for tissue pathology in Atlantic salmon, Salmo salar, infected with salmonid alphavirus (SAV3).
in Journal of fish diseases
Brady N
(2019)
An immunoturbidimetric assay for bovine haptoglobin.
in Comparative clinical pathology
De Cezaro MC
(2016)
Changes in biochemical analytes in calves infected by nematode parasites in field conditions.
in Veterinary parasitology
Eckersall P
(2019)
Calibration of Novel Protein Biomarkers for Veterinary Clinical Pathology: A Call for International Action
in Frontiers in Veterinary Science
Eckersall PD
(2019)
Review: Proteomic approaches to control lactational parameters in dairy cows.
in Animal : an international journal of animal bioscience
Ferrazza RA
(2017)
Quantitative proteomic profiling of bovine follicular fluid during follicle development.
in Biology of reproduction
Horvatic A
(2019)
Quantitative proteomics using tandem mass tags in relation to the acute phase protein response in chicken challenged with Escherichia coli lipopolysaccharide endotoxin.
in Journal of proteomics
Marcos-López M
(2017)
A proteomic approach to assess the host response in gills of farmed Atlantic salmon Salmo salar L. affected by amoebic gill disease
in Aquaculture
| Description | Antibody has been developed to interact with bovine haptoglobin and chicken alpha 1 acid glycoprotein which are biomarkers of disease in these species. Tests for bovine haptoglobin have been developed for rapid analysis in a laboratory and also for an on-farm test for measuring this protein in milk and could be a potential tool for diagnosis of mastitis the major infectious disease of dairy cows. The use of haptoglobin as a health marker at drying off has been assessed using samples from farms and shown to be better than current test procedures to detect bovine mastitis. The tests for haptoglobin in cattle, ovotransferrin in chicken have been applied to samples from farms and shown that there is diagnostic value in measuring them in samples for health assessments. A novel serum precipitation test for salmon health has been evaluated and assessed in diseases in addition to pancreas disease and has promise for use in diagnosis. |
| Exploitation Route | The findings in dairy cows could be put to use by farmers and vets to identify mastitis and allow early threatment. In chickens the detection of teh biomarker AGP means that carcasses from chicken with fever from infection can be detected. In salmon diseases causing muscle loss and therefore an economic damage to industry can be detected at an early stage. |
| Sectors | Agriculture, Food and Drink |
| Description | Findings on diagnostic testing based on specific protein precipitation have been patented applied for. Project applied for to Scottish Aquaculture Innovation Centre SAIC has funded the project PhD projects funded from other sources have been initiated |
| First Year Of Impact | 2021 |
| Sector | Agriculture, Food and Drink |
| Impact Types | Economic |
| Description | BBSRC IAA University of Glasgow |
| Amount | £300,000 (GBP) |
| Funding ID | BB/S506734/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2018 |
| End | 03/2022 |
| Description | BBSRC Impact Accelerator Award via University of Glasgow |
| Amount | £6,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 11/2015 |
| End | 07/2016 |
| Description | Collaborative research project |
| Amount | £414,546 (GBP) |
| Funding ID | CMS2 |
| Organisation | Scottish Aquaculture Innovation Centre |
| Sector | Multiple |
| Country | United Kingdom |
| Start | 08/2021 |
| End | 11/2022 |
| Description | Rapid, accurate, on-farm diagnosis and monitoring of mastitis in dairy cows |
| Amount | £633,445 (GBP) |
| Funding ID | 104295 |
| Organisation | Innovate UK |
| Sector | Public |
| Country | United Kingdom |
| Start | 05/2018 |
| End | 11/2020 |
| Description | Seeding Catalyst Award |
| Amount | £19,450 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 11/2017 |
| End | 02/2018 |
| Title | Effect of pre-analytical treatments on bovine milk acute phase proteins |
| Description | |
| Type Of Material | Database/Collection of data |
| Year Produced | 2016 |
| Provided To Others? | Yes |
| Title | METHOD FOR DETERMINING PATHOLOGICAL TISSUE DAMAGE AND DIAGNOSING INFECTIOUS DISEASE IN FISH |
| Description | The present invention relates to methods for determining pathological tissue damage in fish. Also the present invention relates to methods for diagnosing infectious disease in fish. In particular, the present invention is based on the surprising finding that mixing fish serum with an aqueous protein precipitation solution according to the present invention will result in a protein precipitation reaction if the fish is suffering from pathological tissue damage. Moreover it was surprisingly observed that the degree of turbidity of the precipitate in the mix of sample and aqueous protein precipitation solution was dependent on the state of infectious disease in fish. |
| IP Reference | WO2016102592 |
| Protection | Patent application published |
| Year Protection Granted | 2016 |
| Licensed | Commercial In Confidence |
| Impact | Diagnostic test for pancreas disease in salmon |
| Title | METHODS FOR DETERMINING PATHOLOGICAL TISSUE DAMAGE IN HIGHER VERTEBRATES |
| Description | The present invention relates to methods for determining and/or identifying and/or screening and/or monitoring pathological tissue damage in a higher vertebrate or a population of higher vertebrates. Also disclosed are a method for determining the stage and/or the severity of pathological tissue damage in a higher vertebrate or a population of higher vertebrates and/or for monitoring pathological tissue damage in a higher vertebrate or a population of higher vertebrates. |
| IP Reference | WO2017109086 |
| Protection | Patent application published |
| Year Protection Granted | 2017 |
| Licensed | Commercial In Confidence |
| Impact | Diagnostic test for muscle damage in aniamls |
| Description | Radio interview on Good Morning Scotland, |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Radio interview on Good Morning Scotland on the discovery of a novel test for pancreas disease in fish |
| Year(s) Of Engagement Activity | 2017 |