Chemo-enzymatic Production of Specialty Glycans

Lead Research Organisation: University of Leeds
Department Name: Sch of Chemistry

Abstract

This project will investigate the enzymatic glycosylation of modified carbohydrate monosaccharides to achieve larger, biologically relevant glycan structures. This will be achieved by extensive screening of glycosyltransferase enzymes (enzymes that catalyse the addition of a new monosaccharide to a growing sugar 'acceptor'), that are able to use modified sugars as the 'acceptor', but also as 'donor'.
The biological relevance of the synthesised new type of glycan structures will be validated by investigating their binding profile to a range of proteins that are known to be of relevance in the health sciences, with the immediate aim to develop new diagnostics applications. However, depending on the array results, further applications in other areas in the health sciences are envisaged.

Technical Summary

The vision of the project centres around the translation of traditional and novel methodologies to develop sustainable and versatile platforms for the industrial production of glycans on scale. We will demonstrate the technology by targeting a set of novel glycan materials that are anticipated to have significant transformational potential in human health and disease in diagnostics and glycoengineering applications. This project requires an integrated approach featuring: 1) synthetic chemistry (unnatural building blocks) 2) biocatalysis (enzymatic synthesis of larger glycans) 3) showcasing glycan potential in diagnostics (glycoarray based platforms). The significant anticipated progression of both biocatalysis and glycoarray technologies will reduce commercialisation barriers both in terms of commercial glycan production and 'first in the market' glycan array companies.

Planned Impact

As described in proposal submitted to IUK

Publications

10 25 50
 
Description We have identified novel enzymes that can join together unnatural carbohydrates
Exploitation Route For development of novel diagnostic tools for a wide variety of diseases
Sectors Chemicals,Healthcare

 
Description Chemo-enzymatic Production of Specialty Glycans 
Organisation Carbosynth Ltd
Country United Kingdom 
Sector Private 
PI Contribution enzymatic synthesis of carbohydrates
Collaborator Contribution synthetic chemistry; microarray construction and analysis
Impact Outputs ongoing. synthetic chemistry; enzymology; molecular biology; microarray analysis
Start Year 2015
 
Description Chemo-enzymatic Production of Specialty Glycans 
Organisation Dextra Laboratories
Country United Kingdom 
Sector Private 
PI Contribution enzymatic synthesis of carbohydrates
Collaborator Contribution synthetic chemistry; microarray construction and analysis
Impact Outputs ongoing. synthetic chemistry; enzymology; molecular biology; microarray analysis
Start Year 2015
 
Description Chemo-enzymatic Production of Specialty Glycans 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution enzymatic synthesis of carbohydrates
Collaborator Contribution synthetic chemistry; microarray construction and analysis
Impact Outputs ongoing. synthetic chemistry; enzymology; molecular biology; microarray analysis
Start Year 2015
 
Description Chemo-enzymatic Production of Specialty Glycans 
Organisation Ludger Ltd
Country United Kingdom 
Sector Private 
PI Contribution enzymatic synthesis of carbohydrates
Collaborator Contribution synthetic chemistry; microarray construction and analysis
Impact Outputs ongoing. synthetic chemistry; enzymology; molecular biology; microarray analysis
Start Year 2015
 
Description Chemo-enzymatic Production of Specialty Glycans 
Organisation Open University
Country United Kingdom 
Sector Academic/University 
PI Contribution enzymatic synthesis of carbohydrates
Collaborator Contribution synthetic chemistry; microarray construction and analysis
Impact Outputs ongoing. synthetic chemistry; enzymology; molecular biology; microarray analysis
Start Year 2015
 
Description Chemo-enzymatic Production of Specialty Glycans 
Organisation Prozomix Ltd.
Country United Kingdom 
Sector Private 
PI Contribution enzymatic synthesis of carbohydrates
Collaborator Contribution synthetic chemistry; microarray construction and analysis
Impact Outputs ongoing. synthetic chemistry; enzymology; molecular biology; microarray analysis
Start Year 2015
 
Description Chemo-enzymatic Production of Specialty Glycans 
Organisation University of Bristol
Country United Kingdom 
Sector Academic/University 
PI Contribution enzymatic synthesis of carbohydrates
Collaborator Contribution synthetic chemistry; microarray construction and analysis
Impact Outputs ongoing. synthetic chemistry; enzymology; molecular biology; microarray analysis
Start Year 2015
 
Description Chemo-enzymatic Production of Specialty Glycans 
Organisation University of Manchester
Country United Kingdom 
Sector Academic/University 
PI Contribution enzymatic synthesis of carbohydrates
Collaborator Contribution synthetic chemistry; microarray construction and analysis
Impact Outputs ongoing. synthetic chemistry; enzymology; molecular biology; microarray analysis
Start Year 2015
 
Description Chemo-enzymatic Production of Specialty Glycans 
Organisation University of Southampton
Country United Kingdom 
Sector Academic/University 
PI Contribution enzymatic synthesis of carbohydrates
Collaborator Contribution synthetic chemistry; microarray construction and analysis
Impact Outputs ongoing. synthetic chemistry; enzymology; molecular biology; microarray analysis
Start Year 2015
 
Description Chemo-enzymatic Production of Specialty Glycans 
Organisation University of Warwick
Country United Kingdom 
Sector Academic/University 
PI Contribution enzymatic synthesis of carbohydrates
Collaborator Contribution synthetic chemistry; microarray construction and analysis
Impact Outputs ongoing. synthetic chemistry; enzymology; molecular biology; microarray analysis
Start Year 2015
 
Description Chemo-enzymatic Production of Specialty Glycans 
Organisation University of York
Country United Kingdom 
Sector Academic/University 
PI Contribution enzymatic synthesis of carbohydrates
Collaborator Contribution synthetic chemistry; microarray construction and analysis
Impact Outputs ongoing. synthetic chemistry; enzymology; molecular biology; microarray analysis
Start Year 2015
 
Description Pint of Science - Complex Life of Sugars 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Presentation and scientific demonstrations for the general public held in a Leeds pub during Leeds Pint of Science Week
Year(s) Of Engagement Activity 2017