DNA-directed construction of three-dimensional photosynthetic assemblies

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This research programme seeks to establish a working platform that will assemble photosynthetic proteins within DNA nanostructures. A hallmark of our approach is to use engineered photosynthetic proteins that selectively bind to target DNA sequences - both single-stranded and double-stranded - within a DNA nanostructure. This sequence selectivity directs the assembly of these proteins within a DNA matrix, thus providing spatial and positional control. Additional positional control of the overall nanostructure will then be imparted by directing the immobilization of the DNA-photosynthetic complexes by nanolithography. This bio-inspired platform methodology merges the principles of "bottom up" DNA nanotechnology with "top down" nanolithography and would provide the means to control, for the first time, the location of each photosynthetic protein module, inter-module distance and their relative orientation in both two- (2D) and three-dimensions (3D) along surfaces. Furthermore, this new design lexicon, if successful, will provide a framework to correlate how these parameters influence overall light harvesting efficiency.

Potential Economic Impact of this Research

Synthetic Biology - One of the fundamental drivers of this research programme is the synthesis of modified DNA and DNA-binding molecules. Both synthetic aspects will deliver a battery of new methods to prepare novel compounds that could have wider economic impact on the Synthetic Biology and Diagnostic sectors of Life Science research beyond the lifespan of this 3 year project.

Diagnostics - New methodology to direct the assembly of DNA nanostructures on solid surfaces will pose additional benefits to the medical diagnostic sectors, particularly those directly associated with the identification of nucleic acid and protein biomarkers. It is envisaged that the benefits will arise from the development of new surface modification chemistries, new methods to incorporate functionality into DNA, which in turn could improve the sensitivity and selectivity of new biomolecular analytes and novel techniques to detect analytes using optical methods.

Outputs: Patent protection of Intellectual Property; exploration of licensees.

Mechanisms of delivering Economic Impact: UoS's and UoG's Knowledge Exchange partnership and Impact Acceleration Account (IAA), Scottish Enterprise Proof of Concept or the Technology Strategy Board's Biomedical Catalyst; Collaborative PhD students through Biomolecular Devices CDT (UoS).

Potential Societal Impact of this Research

This research describes a Synthetic Biology-inspired rationale to produce functional DNA nanostructures. Synthetic Biology has been designated a priority research area by the British Government and "has the ability to revolutionize major industries in bio-energy and bio-technology in the UK" (David Willets). At present, the application of Synthetic Biology techniques to create functional DNA-directed devices has not been recognized as a potential area for wider exploitation. Therefore the potential societal impact could be very significant. In order to prepare for this, public engagement with stakeholders associated with the field of UK Synthetic Biology would expedite acceptance of new and emerging applications.

Outputs: Public discussion of the role of Synthetic Biology and Nanotechnology in society; Dissemination of results through the mainstream press, UoG and UoS websites.
Mechanisms of delivering Societal Impact: Delivery of a Coffee House Lecture in Glasgow's City Centre; Involvement in Glasgow Science Festival and Glasgow Science Centre events.