15-IWYP -Wider and faster: high-throughout phenotypic exploration of novel genetic variation for breeding high biomass and yield in wheat

Lead Research Organisation: University of Nottingham
Department Name: Sch of Biosciences

Abstract

With the global population set to reach nine billion by 2050, there is an urgent need to increase food production by at least 60%. Wheat production has plateaued in many areas of the world due to a lack of novel genetic variation for agronomically important traits compounded by the effects of climate change. The lack of genetic variation in modern wheat is a direct consequence of significant genetic bottlenecks during its evolution. As a result, the amount of genetic variation for agronomically important traits available in wild ancestors and landraces of wheat is significantly greater than in present day elite varieties. In contrast to modern wheat, its distant relatives provide a vast resource of genetic variation for potentially all agronomically important traits, including photosynthesis. However, only a fraction of the genetic variation available has been exploited in breeding programmes.
There is now recognition that improved grain yields of major crops require enhanced total dry weight production which must arise mostly from an improvement of radiation-use efficiency (RUE). Raising RUE requires a higher leaf and canopy photosynthesis rate and this remains an important target to underpin future yield progress. There is compelling evidence that C3 photosynthesis is not optimized for high biomass and moreover there is unrealized genetic potential.
The fastest way to raise photosynthesis by genetic improvement in wheat is to seek existing variation in relevant traits that can be used for breeding and pre-breeding. This may represent new variation in photosynthesis that was either lost from the wheat genome during breeding or never present. Traits include (1) properties of Rubisco (2) regulation of Rubisco activity, (3) photoprotective processes (4) electron transport/ RuBP regeneration (5) photorespiration (6) canopy structure and distribution of photosynthesis and pigments (7) internal leaf and chloroplast morphology (8) stomatal responses (to soil and atmospheric water) and leaf water use efficiency. objective of the programme at Nottingham is to transfer small chromosome segments from related species which carry a target gene but lack any deleterious genes, into wheat.
In this project we will generate, using wide crosses, landraces and existing cultivars, lines with substantial variation in their photosynthetic properties and use high throughput screening techniques to identify progeny with enhanced photosynthetic capacity and efficiency. We will investigate the genetic basis of the photosynthetic variation and with the IWYP HUB integrate these discoveries into a pre-breeding and breeding strategy.
The programme will bring together a multi-disciplinary research team: UoN (wide crossing, wheat physiology, photosynthesis phenotyping), (UoB: Genetic marker analysis), University of Essex (UoE: novel photosynthesis phenotyping, Calvin cycle engineering), Lancaster University Environment Centre (LEC: whole plant carbon gain and water use, Rubisco engineering), CIMMYT (field phenotyping and genotyping).

Technical Summary

Wheat's distant relatives provide a vast resource of genetic variation for potentially all agronomically important traits, including photosynthesis. However, only a fraction of the genetic variation available has been exploited in breeding programmes. The main reason for this has been the inability to detect and characterise genetic material (introgressions) from a wheat relative. However, in the BBSRC funded Wheat Improvement Strategic Programme (WISP), the Universities of Nottingham (UoN) and Bristol (UoB) in collaboration with Affymetrix, have developed an array (based on SNP markers) composed of 35,000 markers polymorphic between elite wheat and the ancestral and distant relatives we are working with. This technology, in combination with molecular cytogenetics, is for the first time enabling large-scale, high throughput production and detection of introgressed chromosome segments into the wheat genome.

This genetic variation provides a focus for screening for improvement in a vital trait: leaf photosynthesis which in this case means the efficiency with which crop plants convert solar radiation into biomass and yield across a range of key environments. Promising properties have been identified in wild relatives and introgressed lines but the rate of screening for this complex trait must be speeded up. In a UK consortium (Nottingham, Bristol, Sheffield, Lancaster), we will operate a phenotyping cascade to move from high-throughput but low resolution (hundreds of wheat plants per day) to a lower throughput but biologically detailed physiological analysis of selected candidate lines. This will be fed to the field IWYPHUB analysis platform in CIMMYT, Mexico for final confirmation and genetic analysis to inform breeding. To do this we will utilise gas exchange, chlorophyll fluorescence alongside advanced genotyping tools in wild types and back crossed lines.

Planned Impact

Our project clearly meets the International Wheat Yield Partnership's objectives of a transformational programme to enhance biomass production and yield in wheat and thus lead to large breakthroughs in genetic yield potential to improve food production in as short a timeframe as possible. To achieve this, we will coordinate key centres of excellence to create a 'UK virtual crop photosynthesis hub' for high throughput screening and analysis. We will meet all requirements o in particular uncovering new genetic variation in relatives and ancestors of wheat, novel optimized smart screens for photosynthesis. Short term: we will enhance capacity of IWYP hub, donating new introgression material with traits geared toward genetically conferred higher biomass and yield contributing toward the focus on genomic selection. Medium term: we will provide novel high biomass material with both a genetic and a physiological basis that will provide the breakthrough in genetic yield potential required. Long term: The genetic and physiological support provided will enable markers to be used for selection in the prebreeding and breeding phases to move toward farmer delivery.

Publications

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publication icon
Burridge AJ (2017) High-Density SNP Genotyping Array for Hexaploid Wheat and Its Relatives. in Methods in molecular biology (Clifton, N.J.)

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Coulton A (2020) Examining the Effects of Temperature on Recombination in Wheat. in Frontiers in plant science

 
Description The rate of genetic progress in biomass production per unit hectare of wheat must be improved if we are to meet global targets for grain production. However, selection for domestication traits has reduced the genetic variation in modern wheat cultivars, a fact that could severely limit the future rate of genetic gain. Wild relatives and landraces of wheat carry considerably more variation for target traits than modern day wheat varieties including substantial variation in photosynthesis; a direct factor in biomass production. However, only a fraction of the genetic variation available has been exploited in breeding programmes. The main reason for this has been the inability to detect and characterise genetic material (introgressions) from a wheat relative. However current technology being used by the partners in this project and in combination with molecular cytogenetics, is for the first time enabling large-scale, high throughput production and detection of introgressed chromosome segments into the wheat genome.
Photosynthesis is a critical property of crops that urgently needs improvement to underpin higher wheat biomass and drive a higher canopy radiation use efficiency (RUE). This project will significantly increase the photosynthetic efficiency and capacity of wheat, leading to the development of higher yielding elite varieties by exploiting genetic variation present in 1) distant relatives of wheat and 2) landraces no longer available in elite wheat breeding programmes.
The overall aims of this project are to generate, using wide crosses, landraces and existing cultivars, lines with substantial variation in their photosynthetic properties. We will use novel high throughput screening techniques to identify progeny with enhanced photosynthetic capacity and efficiency, robustly expressed across environments, and which increase wheat biomass production and yield. We will investigate the genetic basis of the photosynthetic variation and with the IWYP HUB integrate these discoveries into a pre-breeding and breeding strategy. We will coordinate key centres to create a virtual crop photosynthesis hub for high throughput screening and analysis.
Key Project objectives (O 1-4):
1. Generate, using wide crosses, landraces and existing cultivars, lines with substantial variation in their photosynthetic properties (Work package (WP) 1: germplasm development and exploitation).
2. Use novel high throughput screening techniques to identify progeny with enhanced photosynthetic capacity and efficiency, robustly expressed across environments, and which increases wheat biomass production and yield (WP2: High throughput phenotypic screen and WP3: detailed phenotypic screen).
3. Investigate the genetic basis of the photosynthetic variation and with the IWYP hub integrate these discoveries into a pre-breeding and breeding strategy (WP4 and WP6: Genetic characterization of introgressed lines and WP5:data collection, management).
4. Coordinate key centres of excellence to create a virtual crop photosynthesis hub for high throughput screening and analysis.

The project end date was April 2019. We used a no cost extension from the BBSRC to process samples and lines emerging from the project between April and July 2019 and to prepare further manuscripts for publication.
The following is an executive summary of progress after the final year in 2019:
Project highlights
1. We have identified at least two Lines with wild relative DNA insertions that possess consistent higher photosynthesis (lines from the original 23 'breeder's toolkit' derived from Triticum urartu and Aegilops mutica crosses). These have now been tested in the field at the IWYP hub in 2018 and 2019 and the increased photosynthetic capacity, biomass and grain yield has been confirmed
2. There are now around 150 lines (including the 23 breeder's toolkit lines) available for transfer to CIMMYT for field - testing, awaiting the correct arrangements for transfer. Details below.
3. Nearly 100 wild relatives and accessions have now undergone detailed analysis and we have identified substantial improvement in photosynthetic rate in comparison to elite wheat (up to 30 %). These are the subject of a substantial paper about to be submitted to New Phytologist. Variation in Rubisco enzyme properties from wild relatives were shown to have potential improvement over wheat. Many wild relatives showed a superior (faster) response of stomata to step changes in light in comparison to elite wheat, indicating a potential photosynthetic advantage in field conditions
4. Since 2017 over 300 wheat wild relative accessions and species have now been genotyped. These data have been used to identify priority accessions according to divergence.
5. We have published 3 high profile papers outlining new advances in dynamic photosynthesis phenotyping, funded through this IWYP project. We have one about to submit to New Phytologist (characterizing the wild relatives) and one field paper in preparation (high photosynthesis in the DH lines at the Hub).

2019 Field analysis of DH lines generated at Nottingham transferred to the IWYP Hub

Last year we reported on an incomplete, ongoing data set. Here we complete the data description. Qe must emphasise that the complete data set is substantially greater, with in depth diurnal analysis on gas exchange, leaf morphology, chlorophyll fluorescence. These data will be shared with IWYP. Here we report on the key data as it relates to photosynthesis, biomas and yield.
Previous analyses in the UK indicated that key DH lines generated from T.urartu and T.mutica showed superior photosynthetic capacity in some lines. However, the mild and constant conditions of a glasshouse in the UK are very different to the fluctuating field conditions in Mexico. We therefore considered it important to analyse the responses of these lines in the field and we spent considerable time and resources on field analyses in both 2018 and 2019. Lorna McAusland traveled to Obregon Feb - March 2018 and 2019 to carry out analyses in the field. We shipped a Licor6400XT across from the UK in order to achieve this. 20 DH lines were planted in the field in 2018 and 2019. Data from 2018 indicated one line with higher light saturated photosynthesis (line 20), however there was variable phenology. Data from 2019 (collected in the few days before this report was submitted) used plants grown in the field with less phenological variation due to a more rigorous vernalisation response. These indicate higher rates of photosynthesis and key ACi parameters (Vcmax and Jmax) in line 17 when compared to the wheat parent Pavon76 (fig 2 and 3). Lower dark respiration rates were observed in lines 17 and 20, in comparison with Borlaug100. We observed around 10 % enhancement of biomass and yield in these lines.

Variation in key photosynthesis traits in wheat wild relatives
The wild relatives of modern wheat represent an under-utilised source of genetic and phenotypic diversity. Crossing wild relatives into elite cultivars introduces the potential for wide adaptation to changing environmental conditions, ultimately aiming to improve the stability and capacity for higher productivity. A 2.3-fold variation for flag leaf light and CO2- saturated rates of photosynthesis (Amax) was observed across 86 wild relative accessions, with significant, accession-specific variation in maximum rates of carboxylation (Vcmax), electron transport (Jmax) and rubisco activity. In addition to photosynthetic capacity, dynamic responses were also characterised to identify features associated with efficient carbon acquisition in fluctuating environments; finding significant variation in the timings of non-photochemical quenching, the magnitude and rate of stomatal opening and specific leaf area. All wild relatives presented here were genotyped for future exploitation in genome wide association studies (showing..). We conclude that accession-dependent variation exceeds that of genus or species-dependent variation. The breadth of physiological diversity is discussed and key candidates are identified for the introduction of photosynthetic variation in crossing and pre-breeding programs.
Exploitation Route Our project is a transformational programme to enhance biomass production and yield in wheat and thus lead to large breakthroughs in genetic yield potential to improve food production in as short a timeframe as possible. To achieve this, we will uncover new genetic variation in relatives and ancestors of wheat, novel optimized smart screens for photosynthesis. Short term: we will donate new introgression material with traits geared toward genetically conferred higher biomass and yield contributing toward the focus on genomic selection. Medium term: we will provide novel high biomass material with both a genetic and a physiological basis that will provide the breakthrough in genetic yield potential required. Long term: The genetic and physiological support provided will enable markers to be used for selection in the prebreeding and breeding phases to move toward farmer delivery. This will include data collected from the wild relatives themselves (see above).
Socioeconomic impact and and food security :
1. Impact at the farmer level:
Yields of wheat in developing regions of the world are essential to economic and socioeconomic security for farming communities. The traits we are focussing on here are intended to be effective across different mega environments and so have the greatest chance of improving livelihoods in these regions.
2. Impact on global hunger and food security:
Wheat provides about 20% of all calories consumed by people worldwide and also makes a significant contribution to animal feed. Importantly demand for wheat is predicted to increase in the near future as the global population increases and the area of available land for agriculture decreases. Therefore wheat production will have an immense impact on food security and the global economy in the coming decades.
Sectors Agriculture, Food and Drink,Environment

URL http://www.cerealsdb.uk.net/cerealgenomics/CerealsDB/IWYP_blast.php
 
Description Problems caused by introgessions
Geographic Reach Multiple continents/international 
Policy Influence Type Influenced training of practitioners or researchers
Impact Wheat breeding companies were made aware about the effect that introgressions have on recombination and why this may lead to difficulties in the future, this has caused some companies to re-examine their breeding strategies
 
Description BBSRC Flexible Talent Mobility Award (FTMA)
Amount £25,000 (GGP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 08/2019 
End 12/2019
 
Description International workshops
Amount £10,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 07/2019 
End 08/2019
 
Description iCASE DTP
Amount £71,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2017 
End 10/2021
 
Title High throughput chlorophyll fluorescence dynamics for wheat 
Description We have developed a method for the high throughput analysis of chlorophyll fluorescence (CF) , under fluctuating light, of leaf samples or whole plants. This allows kinetics of induction and relaxation of CF of a large number of leaf samples (typically up to 70) within one or two hours. Previously this would have taken one or two hours per sample. This method is bring written into a paper for submission for peer review : 'Title: A Rapid, high through-put procedure utilising chlorophyll fluorescence imaging to screen/ phenotype cereal leaf dynamic photosynthesis under controlled gaseous conditions' 
Type Of Material Biological samples 
Year Produced 2018 
Provided To Others? Yes  
Impact Method complete, still applying current research. 
 
Title New markers and new genotyping platform for wheat breeding 
Description New Axiom markers for use in wheat breeding 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? Yes  
Impact New markers and new GbyS genotyping platform for screening and genotyping wheat 
 
Title Whole plant gas exchange measurements 
Description Here we describe a whole plant gas exchange system that can rapidly alter evaporative demand when measuring An, Tr and intrinsic WUE (iWUE) and identify genetic variation in this response. An was not limited by VPD under steady-state conditions but some wheat cultivars restricted Tr under high evaporative demand, thereby improving iWUE. These changes may be ABA-dependent, since the barley ABA-deficient mutant (Az34) failed to restrict Tr under high evaporative demand. Despite higher Tr, Az34 showed lower An than wild-type (WT) barley because of limitations in Rubisco carboxylation activity. Tr and An of Az34 were more sensitive than WT barley to exogenous spraying with ABA, which restricted photosynthesis via substrate limitation and decreasing Rubisco activation. 
Type Of Material Physiological assessment or outcome measure 
Year Produced 2018 
Provided To Others? Yes  
Impact Examining whole plant gas exchange responses to altered VPD can identify genetic variation in whole plant iWUE, and facilitate an understanding of the underlying mechanism(s). 
URL https://plantmethods.biomedcentral.com/articles/10.1186/s13007-018-0357-9
 
Title deriving stomatal conductance from thermal imaging in a dynamic environment 
Description In spite of the significant progress made in recent years, the use of thermography to derive biologically relevant traits remains a challenge under fluctuating conditions. The aim of this study was to rethink the current method to process thermograms and derive temporal responses of stomatal conductance (gsw) using dynamic energy balance equations. Time-series thermograms provided the basis for a spatial and temporal characterisation of gsw responses in wheat (Triticum aestivum). A leaf replica with a known conductance was used to validate the approach and to test the ability of our model to be used with any material and under any environmental conditions. The results highlighted the importance of the coordinated stomatal responses that run parallel to the leaf blade despite their patchy distribution. The diversity and asymmetry of the temporal response of gsw observed after a step increase and step decrease in light intensity can be interpreted as a strategy to maximize photosynthesis per unit of water loss and avoid heat stress in response to light- flecks in a natural environment. 
Type Of Material Physiological assessment or outcome measure 
Year Produced 2019 
Provided To Others? Yes  
Impact This study removes a major bottleneck for plant phenotyping platforms and will pave the way to further developments in our understanding of stomatal behaviour. 
URL https://watermark.silverchair.com/erz068.pdf?token=AQECAHi208BE49Ooan9kkhW_Ercy7Dm3ZL_9Cf3qfKAc485ys...
 
Title Axiom® Wheat Relatives Array data 
Description The array was developed in collaboration with the University of Bristol. More information about the SNPs on the array and development of other molecular marker resources in wheat can be found on the CerealsDB website. A number of chromosome-specific KASP™ assays have been developed for rapid detection of wild relative introgressions into wheat. These assays also allow the identification of the site of introgression in wheat and enable us to differentiate between homozygous and heterozygous introgressions. 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact TBA 
URL https://www.nottingham.ac.uk/wrc/germplasm-resources/genotyping.aspx
 
Title CerealsDB 
Description The CerealsDB web-site was created by members of the Functional Genomics Group at the University of Bristol. The site provides a range of facilities for the study of the wheat genome. The site has been designed with breeders in mind, and we hope that is will be easy and straightforward to use. 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact There have been over 1,528,817 unique visits to the websites and our various datasets have been downloaded 48,295 times (35K Wheat breeders Array: 45,445 downloads and 820K High Density Wheat Array; 2,850 downloads). The trend of increased numbers of researchers visiting our web site shows no sign of slowing down; running as it is at greater than 50,000 unique visits per month. 
URL http://www.cerealsdb.uk.net/
 
Title Introgression plotter added to Cerealsdb 
Description Software to allow users to screen wheat germplasm for potential introgressions from wheat relative 
Type Of Material Data analysis technique 
Year Produced 2019 
Provided To Others? Yes  
Impact At the request of the wheat breeding companies we have made this tool available via Cerealsdb so that breeders can check on the presence of possible introgressed regions in the bread wheat genome 
 
Title QTL database 
Description Upload of QTL database to Cerealsdb 
Type Of Material Data analysis technique 
Year Produced 2019 
Provided To Others? Yes  
Impact Breeders and academics are now able to download/examine various QTL-based datasets 
 
Title Upgrade to cerealsdb (cerealsdb3) 
Description We have carried out a significant upgrade of cerealsdb to include new analytical tools and a much larger data set 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact Increased level of data downloads and use of tools to detect introgressions in wheat 
URL http://www.cerealsdb.uk.net/cerealgenomics/CerealsDB/indexNEW.php
 
Description Collaboartion with Syngenta to exchnage knowledge on trasformation and double haploid production 
Organisation Syngenta International AG
Department Syngenta Ltd (Bracknell)
Country United Kingdom 
Sector Private 
PI Contribution exchange of personal to improve our technology in double haploid production
Collaborator Contribution Spent time at Syngenta providing input into transformation technolgy
Impact Better transformation and double haploid technology available to Bristol
Start Year 2019
 
Description Maize and Wheat Improvement Centre (CIMMT) 
Organisation International Centre for Maize and Wheat Improvement (CIMMYT)
Country Mexico 
Sector Charity/Non Profit 
PI Contribution CIMMYT provide scientific expertise and field trial facilities for wheat research. It operates as a scientific collaboration on some projects and a sub contract for field trials on others. We work with Prof Matthew Reynolds and Dr GEmma Molero.
Collaborator Contribution Scientific expertise and projects funded by UKRI on wheat photosynthesis and wheat physiology.
Impact Outcomes expected in 2020.
Start Year 2015
 
Description Academic visitor from the University of Salamanca 3/9/2018 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Academic visit from Javier Bobo Pinilla of the University of Salamanca. Visit was for training, data sharing and discussion of future collaboration opportunities
Year(s) Of Engagement Activity 2018
 
Description Agritech Event (Essex) to show case research activities to the industry 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact To showcase and describe research funding at Essex as well as our expertise and capabilities for interacting with the agriculture sector in order to facilitate collaborative research across the faculty and industry.
Year(s) Of Engagement Activity 2018
URL https://www.agritech-east.co.uk/agri-tech-member/university-of-essex/#
 
Description Annual IWYP Conference attendance and presentation 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact https://iwyp.org
This is the annual meeting of the International Wheat Yield Consortium , held at the John Innes centre in June 2018. Erik Murchie and Keith Edwards presented the findings of this IWYP project.
Year(s) Of Engagement Activity 2018
 
Description Attendance of IWYP annual conference 2017-03-19 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Amanda Burridge attended IWYP annual conference and discussions.
Year(s) Of Engagement Activity 2017
 
Description Bristol Plant Science Workshop, 2018-10-17 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Amanda Burridge attended the Bristol Plant Science Workshop and presented 'Using Ancient Grains to Improve Modern Bread Wheat'
Year(s) Of Engagement Activity 2018
 
Description CIMMYT visit to IWYP material Field Trials 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Amanda Burridge visited the Obregon CIMMYT site, specifically field sites used for IWYP projects.
Year(s) Of Engagement Activity 2017
 
Description Chairing a session at the Annual SEB meeting 2019 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Co-chairing an SEB session on Stomata and photosynthetic regulation of water use efficiency at the annual SEB meeting in Seville.
Year(s) Of Engagement Activity 2019
URL http://www.sebiology.org/events/event/seb-seville-2019/programme/plant-biology#stomatal
 
Description Engagement with industry 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Meeting with technology provider regarding a new genotyping platform and its application
Year(s) Of Engagement Activity 2017
 
Description Engagement with industry 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Meeting with service providers for genotyping technical development
Year(s) Of Engagement Activity 2017
 
Description Engagement with industry 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Germplasm exchange for technical development
Year(s) Of Engagement Activity 2017
 
Description Engagement with industry 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Transfer of markers to technology provider to aid the development of a new genotyping platform
Year(s) Of Engagement Activity 2017
 
Description Engagement with industry 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Discussion with technology provider regarding optimisation of new technology for wheat
Year(s) Of Engagement Activity 2018
 
Description Engagement with industry 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Discussion with breeder regarding marker development for tracking introgressions
Year(s) Of Engagement Activity 2017
 
Description From Seed to Pasta III, Bologna, Italy, 2018-09-19 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Amanda Burridge attended and met delegates from From Seed to Pasta III, a conference that focused on durum wheat breeding and processing.
Year(s) Of Engagement Activity 2018
 
Description Gatsby masterclass 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Practical activity in which A level student extracted DNA from GM plant material and carried out PCR to check for gene of interest. This resulted in questions and discussions regarding the methods and the importance of plant science and "feeding the world".
Year(s) Of Engagement Activity 2019
 
Description IWYP annual meeting and workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact https://iwyp.org This is the annual meeting of the International Wheat Yield Consortium , held at CIMMYT, Oregon, Mexico in 2019. Erik Murchie presented the findings of this IWYP project.
Year(s) Of Engagement Activity 2019
 
Description Keynote presentation at 2019 Monogram 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Keynote, plenary lecture at Monogram 2019
Year(s) Of Engagement Activity 2019
 
Description Life Sciences Symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Other audiences
Results and Impact Scientists from all levels across different fields of Life sciences discussed the possibilities for collaboration. Many discussed a new interest in food security.
Year(s) Of Engagement Activity 2018
 
Description Life Sciences Symposium 2018-02-02 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Amanda Burridge presented work by the Bristol Cereal Genomics team.
Year(s) Of Engagement Activity 2018
 
Description Meeting with South African deligation to discuss meiosis, genotyping and wheat breeding 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Meeting with British Council lead South African delegation to discuss wheat breeding and possible further links
Year(s) Of Engagement Activity 2018
 
Description Meeting with international experts in wheat breeding and recombination 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Meeting of international experts in wheat breeding and recombination/marker development to consider future prospects
Year(s) Of Engagement Activity 2018
 
Description Meeting with representatives from South Africa 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Met with representatives from Industry, Research Institutes, Academia and Policy makers to discuss future collaboration.
Year(s) Of Engagement Activity 2018
 
Description Monogram 2018 - John Innes Centre 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Amanda Burridge presented 'Rapid and Affordable Genotyping by Sequencing Optimised for Hexaploid Wheat' at Monogram 2018
Year(s) Of Engagement Activity 2018
 
Description Nominated and selected as Co-chair of the GRC CO2 assimilation, USA 2019, and Chair of GRC on the next conference in Italy May 2021 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Nominated and selected by peer to be the co-chair for the 2019 conference, and chair for the 2021 GRC conference on CO2 assimilation.

https://www.grc.org/co2-assimilation-in-plants-from-genome-to-biome-conference/2019/
https://www.grc.org/co2-assimilation-in-plants-from-genome-to-biome-conference/2021/
Year(s) Of Engagement Activity 2019
URL https://www.grc.org/co2-assimilation-in-plants-from-genome-to-biome-conference/2019/
 
Description Nuffield Research placement provider 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Provided three summer placement funded by Nuffield for students to gain experience of working in a lab. Students built curiosity in plant sciences and in particular stomatal behavior. They had the opportunity to experience a real research lab environment.
Year(s) Of Engagement Activity 2017
 
Description Organise meeting of the UK Rice Research Community 2019 at University of Nottingham 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact At U. Nottingham, I co organised the second meeting of the UKRRC. This is due to be held on the 2nd and 3rd May 2019.
The UK Rice Research consortium (UKRRC) has been established to highlight the breadth and quality of research in UK's based research institutions on rice, and to provide a focal point for building new networks both within the UK and with international partners.

Analysis shows that in previous years rice has mostly been used as a model organism for basic plant research, but recently this has changed to more applied research. For much of the world's poor, rice (O. sativa) provides the majority of daily calories. Rice productivity has more than doubled in recent decades, resulting from continued breeding efforts. However, to meet the demands imposed by the projected increase in population, rice production has to continue growing rapidly, while meeting challenges imposed by a changing climate. With the recent sequencing of >3000 different varieties, there is a huge genetic resource available for identifying polymorphisms associated with desirable traits e.g. tolerance to biotic or abiotic stress, yield, nutritional content etc., which in due course can be bred into major crop varieties.

The UKRRC is building upon genomic resources and large-scale phenotyping platforms, and works with international partners on fundamental science and applied breeding programmes to tackle food security challenges, as highlighted under Research and Partnerships.
Year(s) Of Engagement Activity 2019
URL http://ukrrc.org
 
Description Organising Monogram 2019 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Erik Murchie cp - organised Monogram 2019 which is too be held in April 2019 . The Monogram Network meeting is the annual get together for the small grain cereal and grass research community. Academics, commercial scientists, and plant breeders gather to share the latest advances in scientific research, exchange ideas, and talk about collaboration. Monogram 2019 provides an exciting opportunity for sharing ideas and networking in a relaxed environment. Everyone from graduate students to established academics, commercial scientists and plant breeders are welcome to attend.
Year(s) Of Engagement Activity 2019
URL http://www.monogram.ac.uk/MgNW2019.php
 
Description Plant Development and Evolution International Workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact An International workshop to encourage meaningful exchanges and collaborations between global institutions. After presentation of data and some of the limitations of research, researchers from Zurich and Kyoto University have offered useful advice and potential future collaboration.
Year(s) Of Engagement Activity 2018
 
Description Plant Development and Evolution International Workshop 2018-02-22 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Amanda Burridge attended the Plant Development and Evolution International Workshop in Zurich with the presentation 'Improving Wheat Photosynthesis using Wild Relatives'
Year(s) Of Engagement Activity 2018
 
Description Plant Ecophysiology group workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact The PEP group is a special interest group of the SEB and BES. In 2012 Lawson organised a techniques workshop to train the next generation of scientists in some of the key physiological techniques. The week long event comprised of morning lecturer/seminar for expert researchers in the field followed by hands on practical sessions supported by the Industrial manufacturers. Lawson organised the 2014 sessions and is co-organiser of the 2016 and 2018 events.
Year(s) Of Engagement Activity 2012,2014,2016,2018
URL https://sites.google.com/prod/view/drrjwebster/pepg-workshop
 
Description Plant and Animal Genomes (PAG) XXVI 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Amanda Burridge attended Plant and Animal Genomes (PAG) XXVI and presented 'CerealsDB Version 4.0: A Review of Tools and Data for Wheat Breeders and Research Scientists'
Year(s) Of Engagement Activity 2018
 
Description Progress meeting at Lancaster 2017-11-22 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Amanda Burridge attended and presented data at an IWYP meeting attended by delegates from Nottingham University, Lancaster University and Essex University
Year(s) Of Engagement Activity 2017
 
Description Progress meeting at Nottingham 2017-03-14 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Amanda Burridge and Keith Edwards attended an IWYP progress meeting at Nottingham University with delegates from Nottingham University, Lancaster University and Essex University
Year(s) Of Engagement Activity 2017
 
Description Progress meeting at via Skype 2017-08-23 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Amanda Burridge attended an IWYP Skype meeting with delegates from Nottingham University, Lancaster University and Essex University
Year(s) Of Engagement Activity 2017
 
Description Progress meeting at via Skype 2017-10-17 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Amanda Burridge and Keith Edwards attended an IWYP Skype meeting with delegates from Nottingham University, Lancaster University and Essex University
Year(s) Of Engagement Activity 2017
 
Description Progress meeting at via Skype 2018-01-05 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Amanda Burridge and Keith Edwards attended an IWYP Skype meeting with delegates from Nottingham University, Lancaster University and Essex University
Year(s) Of Engagement Activity 2018
 
Description Progress meeting at via Skype 2018-08-30 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Amanda Burridge attended an IWYP Skype meeting with delegates from Nottingham University, Lancaster University and Essex University
Year(s) Of Engagement Activity 2018
 
Description Progress meeting at via Skype 2018-12-20 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Amanda Burridge and Keith Edwards attended an IWYP Skype meeting with delegates from Nottingham University, Lancaster University and Essex University
Year(s) Of Engagement Activity 2018
 
Description Public dialog and debate 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact A pint of science debate on the price of bread and wheat breeding
Year(s) Of Engagement Activity 2017
 
Description Public lecture in series 'whats it all about' held at Sutton Bonington 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Public lecture on research into improving crop photosynthesis and its wider importance and interest for society and food security . It was held at the University of Nottingham (Sutton Bonington) in April 2015 and open largely to the local public .
Year(s) Of Engagement Activity 2015
 
Description Radio broadcast Farming today 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact Interview for farming today on wheat breeding and its importance; 14th July 2017
Year(s) Of Engagement Activity 2017
 
Description Rank Prize acceptance speech 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Rank prize (2018) acceptance speech
Year(s) Of Engagement Activity 2017
URL http://www.rankprize.org/index.php/prizes/prizes-2018
 
Description Rank Symposium on "The shape of wheat to come" 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Study participants or study members
Results and Impact The Rank Symposium of "The shape of wheat to come" was a four day event organised by Edwards and Higgins to promote the area of recombination to a wide range of international scientists ranging from PhD students to retired professors
Year(s) Of Engagement Activity 2018
 
Description Royal Society Partnership grant which included a 2.5 day visit to local 6th form school (Suffolk one). 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact The Royal Society partnership grant enabled thermal camera to be purchased by the school. Lawson visited the school to present a seminar about the effect of climate change on stomatal behaviour and the use of thermal cameras to monitor plant performance. This was followed by a practical session involving droughted and water watered plants. As part of the assessment the students had to prepare and present posters to explain their findings.
Year(s) Of Engagement Activity 2018
 
Description Royal Society student conference (London RS) December 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact This event followed the Royal Society partnership award that between myself at the University of Essex and Dr Tanja Hofmann One, Ipswich. This event included poster presentation by the school students as well as a display stand to demonstrate the application and benefits of using thermography to assess plant performance.
Year(s) Of Engagement Activity 2018
 
Description School Seminar (Bristol University) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Lunch time seminar showcase research activities and finding based on funded research within the lab.
Year(s) Of Engagement Activity 2018
 
Description School Seminar (Glasgow University) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Lunch time seminar showcase research activities and finding based on funded research within the lab.
Year(s) Of Engagement Activity 2018
 
Description Stakeholder Workshop: Social Responsibility and Wheat Research 13/9/18 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Stakeholders talked about their own interests (and/or those of their community) in relation to the many uses of wheat. The event also considered stakeholder responses as the basis for a report on social responsibility and wheat research targeted at policy audiences
Year(s) Of Engagement Activity 2018
 
Description Teaching workshop -workshop on phenotyping photosynthetic phoneme at U. Wageningen 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Teaching undergraduates and postgraduates and industry representatives about techniques in photoprotection and canopy structure phenotyping.
This resulted in questions and discussions
Year(s) Of Engagement Activity 2014
 
Description Visited VIT India 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Undergraduate students
Results and Impact Presented some basic information on photosynthesis, followed by our research in the area of trying to improve photosynthetic capacity to increase yield. This raised a number of questions regarding the processes, mechanisms and even the use of GM technology and alternatives.
Year(s) Of Engagement Activity 2019
 
Description Visitors from the University of Florence 2-6 July 2018 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Academic visit from Alberto Masoni and Massimo Gori of the University of Florence. Visit was for training, data sharing and discussion of future collaboration opportunities
Year(s) Of Engagement Activity 2018
 
Description Wheats and Women International Conference, National Research Council, Rome, 2018-06-14 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Amanda Burridge attended and presented at the first Women in Wheat (Carlotta Award) conference in Rome.
Year(s) Of Engagement Activity 2018
 
Description invited speaker : CO2 assimilation in Plants from Genome to Biome 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact he growing concern over global food and energy security results from rising population, while land degradation and climate change continue to limit production. One of society's major challenges is to grow more biomass on less land, using less water, fertilizer, fungicides and pesticides than ever before. To accomplish this, a new "green revolution" that surpasses the rate of current crop and fuel production is required and improving photosynthetic CO2 assimilation is key to achieving this goal. The Gordon Research Conference (GRC), CO2 Assimilation in Plants from Genome to Biome, is among the most critical forums for bringing together the top plant scientists in the world, along with rising early-career scientists, to share and discuss the critical advances on this grand challenge. At this meeting scientists will present and discuss cutting edge, often unpublished research, that is geared toward the new "green revolution" and that will develop ideas and collaborations to guide and support research on carbon aspects of photosynthesis for years to come.
Year(s) Of Engagement Activity 2019
URL https://www.grc.org/co2-assimilation-in-plants-from-genome-to-biome-conference/2019/