Japan Partnering Award -Engineering novel transgenic zebrafish with CRISPR/Cas9 technology
Lead Research Organisation:
University of Exeter
Department Name: Biosciences
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Publications
Aya Takesono
(2021)
Estrogen regulates development of the olfactory sensory system in the zebrafish embryo
in E-life
Brown AR
(2019)
Cardiovascular Effects and Molecular Mechanisms of Bisphenol A and Its Metabolite MBP in Zebrafish.
in Environmental science & technology
Gould SL
(2021)
The potential for adverse effects in fish exposed to antidepressants in the aquatic environment.
in Environmental science & technology
Matthew J. Winter
(2021)
Functional imaging in larval zebrafish for characterising the effects of proconvulsant compounds acting via a range of pharmacological mechanisms
in British Journal of Pharmacology
Miyaoku K
(2021)
Characterization of G protein-coupled estrogen receptors in Japanese medaka, Oryzias latipes.
in Journal of applied toxicology : JAT
Moreman J
(2018)
Estrogenic Mechanisms and Cardiac Responses Following Early Life Exposure to Bisphenol A (BPA) and Its Metabolite 4-Methyl-2,4-bis( p-hydroxyphenyl)pent-1-ene (MBP) in Zebrafish.
in Environmental science & technology
Mourabit S
(2019)
New insights into organ-specific oxidative stress mechanisms using a novel biosensor zebrafish.
in Environment international
Ogino Y
(2018)
Functional distinctions associated with the diversity of sex steroid hormone receptors ESR and AR.
in The Journal of steroid biochemistry and molecular biology
| Description | We have developed several different oestrogen biosensor transgenic zebrafish lines which have great utility for advancing our understandings on adverse impacts of oestrogen on brain development. Newly generated transgenic zebrafish lines are listed under "animal use". We have also developed imaging systems for establishing 4D map of neuronal activity in a whole brain of a live zebrafish embryo using LSM as well as zeiss LSM810 Airyscan and for olfaction-mediated behaviour assay systems. These systems are a valuable resource for use in assessing for adverse effects of environmental pollutants on brain development. - Significant new resources through the development of a series of new transgenic fish models for studies into chemical, disease and developmental processes and mechanisms ( see Research Tools and Methods). |
| Exploitation Route | Significant new resources through the development of a series of new transgenic fish models for studies into chemical, disease and developmental processes and mechanisms ( see Research Tools and Methods). By using the CRISPR-mediated gene knock-in method, it is possible to generate more specific genetic mutations in the genome. All of our new transgenic zebrafish are available for use in the wider research community |
| Sectors | Environment,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology,Other |
| Description | (see also Key Findings) - Widespread dissemination of information on the development and application of transgenic fish models via conference presentations and workshops - Exeter provision of transgenic fish models to other research laboratories - UK-Japan partner engagement including academics and government agencies ( Min of Env Japan) through workshops and laboratory visits We have developed several different oestrogen biosensor transgenic zebrafish lines which have great utility for advancing our understandings on adverse impacts of oestrogen on brain development. Newly generated transgenic zebrafish lines are listed under "animal use". We have also developed imaging systems for establishing 4D map of neuronal activity in a whole brain of a live zebrafish embryo using LSM as well as zeiss LSM810 Airyscan and for olfaction-mediated behaviour assay systems. These systems are a valuable resource for use in assessing for adverse effects of environmental pollutants on brain development. Major new grant funding. Pandemic has prevented travel to Japan to link with our partners there. This will result in an underspend on this grant. |
| First Year Of Impact | 2017 |
| Sector | Environment,Healthcare |
| Description | Government report |
| Geographic Reach | Asia |
| Policy Influence Type | Contribution to a national consultation/review |
| Impact | Government report: • Taisen Iguchi and Charles R. Tyler (2020) Defra , UK, Min of Evn, Japan Workshop Report. |
| Description | Government report |
| Geographic Reach | Multiple continents/international |
| Policy Influence Type | Participation in a guidance/advisory committee |
| Description | AZ Discovery Science - Discovery Biology team, leading to a pilot study |
| Amount | £45,000 (GBP) |
| Organisation | AstraZeneca |
| Sector | Private |
| Country | United Kingdom |
| Start | 12/2019 |
| End | 03/2020 |
| Description | Advancing understanding of anaesthesia and analgesia in the zebrafish |
| Amount | £566,940 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 02/2021 |
| End | 01/2022 |
| Description | AstraZeneca Contract Research: zebrafish development assays |
| Amount | £80,000 (GBP) |
| Organisation | AstraZeneca |
| Sector | Private |
| Country | United Kingdom |
| Start | 01/2018 |
| End | 12/2018 |
| Description | BBSRC China - UK Partnering Award: Breeding and health for improving robustness in aquaculture. |
| Amount | £42,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 02/2019 |
| End | 01/2020 |
| Description | Building confidence in non-protected zebrafish embryo-larvae as a viable alternative to mammalian DART assessment |
| Amount | £120,000 (GBP) |
| Funding ID | NC/X001407/1 |
| Organisation | National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2023 |
| End | 03/2027 |
| Description | Characterising the functional homology of drug targets in aquatic animals |
| Amount | £80,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 02/2021 |
| End | 01/2025 |
| Description | Characterising the homology of phamaceutical targets in aquatic life |
| Amount | £80,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 10/2020 |
| End | 09/2024 |
| Description | Development of CRISPR-Cas and its applications in zebrafish |
| Amount | £612,000 (GBP) |
| Organisation | AstraZeneca |
| Sector | Private |
| Country | United Kingdom |
| Start | 04/2020 |
| End | 03/2022 |
| Description | Development of precise genome editing technology in zebrafish |
| Amount | £675,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2023 |
| End | 12/2026 |
| Description | Ecotoxicology of Pharmaceuticals in Fish |
| Amount | € 100,000 (EUR) |
| Organisation | Roche Pharmaceuticals |
| Sector | Private |
| Country | Global |
| Start | 01/2023 |
| End | 12/2025 |
| Description | Effects of water chemistry on the exposure risk for pharmaceutical drugs, including antibiotics |
| Amount | £80,000 (GBP) |
| Organisation | AstraZeneca |
| Sector | Private |
| Country | United Kingdom |
| Start | 10/2018 |
| End | 09/2022 |
| Description | Industry CASE (PhD) - Advancing transgenic zebrafish bioassays for drug analysis using imaging |
| Amount | £100,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2018 |
| End | 12/2021 |
| Description | Investigating the action of AZD9833 -SERD in the estrogen responsive element (ERE) transgenic zebrafish |
| Amount | £41,939 (GBP) |
| Organisation | AstraZeneca |
| Sector | Private |
| Country | United Kingdom |
| Start | 07/2021 |
| End | 06/2022 |
| Description | MRC Confidence in Concept funding/AstraZeneca. The zebrafish as a fast and reliable vertebrate model to study gene function to support target validation in the search for new human drug therapies. |
| Amount | £220,000 (GBP) |
| Organisation | Medical Research Council (MRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2021 |
| End | 08/2023 |
| Description | Purchase of the Acquifer advanced plate imager sponsored by support from UoE, Astrazeneca and Ditabis |
| Amount | £90,000 (GBP) |
| Organisation | AstraZeneca |
| Sector | Private |
| Country | United Kingdom |
| Start | |
| Description | Understanding phenotypic plasticity and relevance of behavioural responses to chronic toxicityu in aquatic life |
| Amount | £0 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 10/2020 |
| End | 09/2024 |
| Description | Understanding plasticity and the ecological relevance of behavioural responses in fish to neuroactive pharmaceuticals |
| Amount | £80,000 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2021 |
| End | 09/2025 |
| Description | • EU/IMI PREMIER |
| Amount | € 8,000,000 (EUR) |
| Organisation | EU-T0 |
| Sector | Public |
| Country | European Union (EU) |
| Start | 01/2020 |
| End | 12/2025 |
| Title | Application and significant advancement of gene targeting in zebrafish model using CRISPR technology |
| Description | Application of CRISPR technology for the gene targeting of a brain specific estrogen synthesising enzyme, Cyp19a1b. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2021 |
| Provided To Others? | Yes |
| Impact | Significant advancement of gene targeting in zebrafish model using CRISPR technology. Application of CRISPR technology in zebrafish has resulted in evidence supporting roles of specific genes being involved in heart disease in humans and providing new transgenic models for studying effect mechanisms of endocrine active chemicals in fish. Application of CRISPR technology for the gene targeting of a brain specific estrogen synthesising enzyme, Cyp19a1b. Application of CRISPR technology has been used to help identify the roles of a new neural cell type - estrogen receptor olfactory bulb - in the sense of olfaction. |
| Title | CRISPR-mediated gene knock-in method |
| Description | Recently CRISPR/Cas9 technology has became a very powerful technology by which genomic DNA sequence can be edited to induce genetic mutations (gene knock-out). However, applying this technique to insert an external DNA fragment into a specific genome locus (gene knock-in) is still technically challenging. By using the CRISPR-mediated gene knock-in method, it is possible to generate more specific genetic mutations in the genome (e.g. tissue and stage specific gene mutation, labelling of gene expression using fluorescent protein). To facilitate such technique, we visited Kawahara lab which is one of the world leading research group in developing CRISPR/Cas9 technologies in zebrafish. In Kawahara lab, we tested a few crRNAs to target genomic DNA in zebrafish (targeting GFP gene in fli-GFP transgenic fish, and FGF2 gene in the wild type zebrafish to optimise the experimental conditions) and attempted to knock down the gene as well as to insert mCherry gene into the same targeted locus in the genome. We quantitatively assessed the activities of crRNAs using genomic PCR and confirmed which sequences of crRNAs effectively edited the host genome. Subsequently, the efficiency of gene knock-in was also assessed by PCR and confirmed that the gene insertion of mCherry transgene at in the Cas9 targeted genomic locus of GFP gene in the fli1-GFP transgenic line. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | By using the CRISPR-mediated gene knock-in method, it is possible to generate more specific genetic mutations in the genome (e.g. tissue and stage specific gene mutation, labelling of gene expression using fluorescent protein). |
| Title | Gene targeting in zebrafish model using CRISPR technology |
| Description | Significant advancement of gene targeting in zebrafish model using CRISPR technology. Application of CRISPR technology for the gene targeting of a brain specific estrogen synthesising enzyme, Cyp19a1b. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2021 |
| Provided To Others? | Yes |
| Impact | - Exeter provision of transgenic fish models to other research laboratories - UK-Japan partner engagement including academics and government agencies ( Min of Env Japan) through workshops and laboratory visits |
| Title | New transgenic fish models |
| Description | • Novel use of transgenic zebrafish we have developed have been applied to a wide series of projects and support major research income from the EU, Industry and RCUK projects ( for work on nanomaterials and pharmaceuticals; see new funding). • Our work on the elav1a transgenic zebrafish has been applied for functional brain imaging in zebrafish for profiling the pharmacology of neuroactive drugs. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | A series of new funded projects co sponsored by EU and various pharmaceutical industry partners |
| Title | Novel use of transgenic zebrafish |
| Description | Ongoing use of previous transgenic lines |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | Novel use of transgenic zebrafish we have developed have continued to be applied to a wide series of projects and support major research income from the EU, Industry and RCUK projects |
| Title | Transgenic Zebrafish models (x3) |
| Description | 1..) Developed a new transgenic zebrafish model [ERE_Gal4ff x UAS_Cre x Ubi_zebrabow] 2..) created a further transgenic zebrafish [ERE_Gal4ff x UAS_ mCherry x UAS _GCaMP6m] 3..) applied our ERE_Gal4ff x UAS_GFP transgenic model |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | 1) Follows the fate and behaviour of oestrogen responsive RGCs in single cell level in larvae and juvenile zebrafish fish brains, investigating also for differences between the sexes. 2) Identified neuronal activity in oestrogen responsive neuronal cells. 3) Investigated effects of exposure to a series of endocrine disrupting chemicals (EDCs) on oestrogen responding neuronal progenitors. |
| Title | Transgenic zebrafish model developed. |
| Description | Line name Origin Usage ERE_GFP Casper University of Exeter (UoE) to establish the role of oestrogens in differentiation and function of neuronal progenitors in the forebrain ERE_NRT_mCherry (ERE_mCherry) UoE, generated to establish the role of oestrogens in differentiation and function of neuronal progenitors in the forebrain, and to induce cell ablation of the oestrogen responsive forebrain cells. ERE_NRT_mCherry x Ubi_Zebrabow UoE, generated to follow the fate and behaviour of oestrogen responsive RGCs in single cell level in larvae and juvenile zebrafish fish brains, investigating also for differences between the sexes. ERE_NRT_mCherry x UAS_GCamp6m UoE, generated to identify neuronal activity in oestrogen responsive neuronal cells. ERE_Kaede UoE generated to establish the role of oestrogens in differentiation and function of neuronal progenitors in the forebrain ERE_NRT_mCherry x Cyp19_GFP UoE generated to establish the role of oestrogens in differentiation and function of neuronal progenitors in the forebrain and to reveal the stage dependency and heterogeneity in oestrogen responsive forebrain cells. EpRE UoE generated To elucidate oxidative stress mechanisms |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | - Widespread dissemination of information on the development and application of transgenic fish models via conference presentations and workshops - Exeter provision of transgenic fish models to other research laboratories - UK-Japan partner engagement including academics and government agencies ( Min of Env Japan) through workshops and laboratory visits - Significant new resources through the development of a series of new transgenic fish models for studies into chemical, disease and developmental processes and mechanisms ( see Research Tools and Methods). |
| Description | Japan Partnering Award |
| Organisation | University of Yamanashi |
| Country | Japan |
| Sector | Academic/University |
| PI Contribution | This project has helped to obtain a Japan Partnering Award: Engineering novel transgenic zebrafish with CRISPR/Cas9 technology. At Exeter, we have applied an oestrogen responsive transgenic zebrafish model and also developed new transgenic models to investigate the roles of oestrogen in neurogenesis and brain development, investigating for sex related differences, including in intact animals in real time using advanced imaging methods. Aya Takesono visited our Japanese partner, Professor Atsuo Kawahara (Medical School, Yamanashi University) from 30/July/2017 to 11/Aug/2017. Tetsu Kudoh also visited Kawahara lab from 21st to 31st August 2017. During the visit, Takesono and Kudoh applied novel CRISPR/Cas9 genome editing technology to zebrafish embryo |
| Collaborator Contribution | Through UK-Japan partnerships we are applying CRISPR/Cas9 to produce novel biosensor transgenic fish and gene knock-outs for studies on hormone receptors in zebrafish. This has lead to a new international research partnership with Professor Atsuo Kawahara (Medical School, Yamanashi University). |
| Impact | We have established new transgenic zebrafish lines to elucidate a novel mechanism of action of oestrogen on the forebrain development, in particular, olfactory development. Through this work, we have discovered a new and earliest target of oestrogen in developing zebrafish; glial progenitor cells in the forebrain that exclusively respond to oestrogen (named ER-forebrain cells) and revealed the roles of these ER-forebrain cells in forebrain development and the associated neuronal networks. Further, we have developed several functional assays to assess the developmental roles of ER-forebrain cells, including immunohistochemical analyses for neuronal activation markers, a live imaging for neuronal activation using a genetically encoded calcium indicator GCaMP system and odour-mediated behaviour assays. |
| Start Year | 2018 |
| Description | Partnership with AstraZeneca |
| Organisation | AstraZeneca |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | CRISPR Cas technology in zebrafish for drug safety analysis /development. |
| Collaborator Contribution | See 2018 return. The project has served to further enable a partnership with AstraZeneca with a major new funded grant from them using CRISPR Cas technology in zebrafish for drug safety analysis /development. |
| Impact | See 2018 return. The project has served to further enable a partnership with AstraZeneca |
| Start Year | 2019 |
| Description | 24th UK-Japan Annual Scientific Workshop |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Prof Charles Tyler was Chair of this workshop and gave the opening talk |
| Year(s) Of Engagement Activity | 2022 |
| Description | Annual Scientific Workshop on Research into Environmental Endocrine Disrupting Chemicals |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Progress in our development and applications of transgenic zebrafish. The 20th UK-Japan Annual Scientific Workshop on Research into Environmental Endocrine Disrupting Chemicals, Downing College, Cambridge. International conference to peers, collaborators, industry, policy making |
| Year(s) Of Engagement Activity | 2019 |
| Description | Aya Takesono: Oral presentation at South West Zebrafish Meeting 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Oral presentation at South West Zebrafish Meeting 2019, 9th September, 2019, University of Bristol, UK. Title: Novel oestrogen-responding glia that regulates olfactory development and function. Authors: Aya Takesono*, Paula Schirrmacher, Aaron Scott, Jon M. Green, Okhyun Lee, Matthew J. Winter, Tetsuhiro Kudoh and Charles R. Tyler |
| Year(s) Of Engagement Activity | 2019 |
| Description | Development and Application of Transgenic Zebrafish for Understanding Chemical and Nanoparticle Exposure Effects The 23rd UK-Japan Annual Scientific Workshop on Research into Environmental Endocrine Disrupting Chemicals & Chemicals of Emerging Concern Virtual meeting |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Annual international research conference/workshop |
| Year(s) Of Engagement Activity | 2021 |
| Description | Government report: The 20th UK-Japan Annual Scientific Workshop on Research into Environmental Endocrine Disrupting Chemicals |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | GOVERNMENT REPORT (DEFRA) |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | In2Science: host a student for a week during summer |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | In2Science (https://in2scienceuk.org/) student host for a week in the summer ~ scheme is aimed at improving social mobility and diversity in science |
| Year(s) Of Engagement Activity | Pre-2006,2019 |
| Description | Invited Opening Keynote Speech for international society for the Developmental Origins of Health and Disease, Melbourne , Australia |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The Chemical Environment and Health. International Society for the Developmental Origins of Health and Disease, Melbourne , Australia, Invited Opening Keynote 22nd October 2019 |
| Year(s) Of Engagement Activity | 2019 |
| Description | Japanese Zebrafish and Medaka conference August 2017 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Tetsu Kudoh gave a key note lecture in the Japanese Zebrafish and Medaka conference on the 29th Aug 2017 (Yamanashi, Japan). Title: 'Molecular mechanisms of embryonic tail development in the self-fertilizing mangrove killifish, Kryptolebias marmoratus' |
| Year(s) Of Engagement Activity | 2017 |
| Description | Opening talk for the UK-Japan Partnership for studies into Endocrine Disrupting Chemicals and Chemicals of Environmental Concern |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | The 22nd UK-Japan Annual Scientific Workshop on Research into Environmental Endocrine Disrupting Chemicals |
| Year(s) Of Engagement Activity | 2020 |
| Description | Oral presentation at 25th Japanese Society for Environmental Toxicology, 25th-27th September 2019, Tsukuba, Japan |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Examining the oxidative toxicity potential of metal based engineered nanomaterials (ENMs) in embryos of an oxidative-stress biosensor transgenic zebrafish. Aya Takesono, Paula Schirrmacher, Sulayman Mourabit, Tetsuhiro Kudoh and Charles R. Tyler (2019) Oral presentation at 25th Japanese Society for Environmental Toxicology, 25th-27th September 2019, Tsukuba, Japan |
| Year(s) Of Engagement Activity | 2019 |
| Description | PRESENTATION: Endocrine Disruption in Aquatic Wildlife - Reproductive Toxicity an Endocrine Disruption. CRU Symposium, Uppsala University, Sweden October 18-19 2018 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | CRU Symposium, Uppsala University, Sweden October 18-19 2018 |
| Year(s) Of Engagement Activity | 2018 |
| Description | Poster presentation at Society of Neuroscience 2019 annual meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Poster presentation at Society of Neuroscience 2019 annual meeting, October 19-23, 2019, Chicago, IL, USA.Title: Estrogen regulates olfactory glomerular development and neuronal activity of the olfactory bulb via specific glial progenitor cells Authors: *A. TAKESONO, P. SCHIRRMACHER, M. J. WINTER, A. SCOTT, J. M. GREEN, O. LEE, H. WAI, T. KUDOH, C. R. TYLER; |
| Year(s) Of Engagement Activity | 2019 |
| Description | Presentation at 23rd UK-Japan Annual Scientific Workshop on Research into Environmental Endocrine Disrupting Chemicals & Chemicals of Emerging Concern - Virtual meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Presentation at 23rd UK-Japan Annual Scientific Workshop on Research into Environmental Endocrine Disrupting Chemicals & Chemicals of Emerging Concern - Virtual meeting |
| Year(s) Of Engagement Activity | 2021 |
| Description | South-west Zebrafish Meeting 2020: Chemicals, Environmental Health and Zebrafish Models, Exeter |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Other audiences |
| Results and Impact | Talks, research findings shared |
| Year(s) Of Engagement Activity | 2020 |
| Description | The Centre for Biomedical Modelling and Analysis Outreach sessions: Using zebrafish to aid the development of safer and more effective human drugs (invited speaker Matt Winter) 2020. |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Other audiences |
| Results and Impact | Dr Matt Winter - invited speaker at an outreach session |
| Year(s) Of Engagement Activity | 2020 |
| Description | The Royal Society Industry Fellows Networking Event 2020 talk by Dr Matt Winter: Exploiting the power of CRISPR/Cas9 gene editing for industry-standard drug target validation |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Talk by Dr Matt Winter: Exploiting the power of CRISPR/Cas9 gene editing for industry-standard drug target validation. Increasing/sharing knowledge |
| Year(s) Of Engagement Activity | 2020 |
| Description | The UK-Japan Partnership for studies into Endocrine Disrupting Chemicals |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Policymakers/politicians |
| Results and Impact | The UK-Japan Partnership for studies into Endocrine Disrupting Chemicals. The 21st UK-Japan Annual Scientific Workshop on Research into Environmental Endocrine Disrupting Chemicals, Otsu, Japan. Opening Address. 12 November 2019 |
| Year(s) Of Engagement Activity | 2019 |
| Description | Waking up to Planetary Health 2020: Chemicals and Environmental Health conference Royal Devon and Exeter Hospital and the University of Exeter Medical School |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Other audiences |
| Results and Impact | Keynote speech 30/04/2020 |
| Year(s) Of Engagement Activity | 2020 |
| Description | kEYNOTE SPEECH: The Feminisation of Freshwater Fish - From Individuals to Population Consequences |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | The Feminisation of Freshwater Fish - From Individuals to Population Consequences. International Symposium on the Reproductive Physiology of Fish, Manaus Brazil. Keynote 3-8th June 2018 |
| Year(s) Of Engagement Activity | 2018 |