Enabling catalytic and quantitative N- and C-terminal protein labelling

Lead Research Organisation: University of Leeds
Department Name: Sch of Chemistry


Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.


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Witkowska D (2018) Analysis of substrate binding in individual active sites of bifunctional human ATIC. in Biochimica et biophysica acta. Proteins and proteomics

Description In this short (3 month) pathfinder project we aimed to develop a protein labelling technology which we had developed within the group. Catalytic and quantitative ways to modify proteins are of interest both for commercial applications and for use in academia. Within the project we carried out both a market analysis for our proposed technology and key proof-of-principle experiments. The proof-of-principle experiments were successful and supported continued investigation of the approach. This is now continuing through a subsequent BBSRC follow-on fund award (BB/R005540/1) which is ongoing. The initial commercial analysis enabled us to identify to identify a potential SME partner for the technology with whom we are now working.
Exploitation Route We have obtained further funding (BB/R005540/1) to develop the initial findings from this pathfinder award into a commercialisable technology.
Sectors Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology

Description The technology developed through this proposal is now being used in industrial collaborations with existing and prospective non-academic research partners. Details of these collaborations are currently confidential.
First Year Of Impact 2020
Sector Pharmaceuticals and Medical Biotechnology
Impact Types Economic

Description BBSRC follow-on fund
Amount £250,000 (GBP)
Funding ID BB/R005540/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2018 
End 09/2019
Description Multivalent display of sugar antigens via site selective enzymatic modification of cholera toxin
Amount £112,000 (GBP)
Organisation GlaxoSmithKline (GSK) 
Sector Private
Country Global
Start 10/2020 
End 09/2024
Description Sortase conjugate vaccines
Amount £39,288 (GBP)
Organisation University of Leeds 
Sector Academic/University
Country United Kingdom
Start 10/2019 
End 03/2020