DERIVE:Dev. of Riboflavin biomarkers to relate dietary sources with status, gene-nutrient interactions and validated health effects in adult cohorts

Lead Research Organisation: University of Ulster
Department Name: Sch of Biomedical Sciences

Abstract

Riboflavin (vitamin B2) is essential energy metabolism, but biomarker
status is rarely measured. The UK and Ireland are the only countries worldwide to have included a riboflavin
biomarker in national dietary surveys. Some concern exists in both countries regarding the large proportion of
adults showing low riboflavin status, measured using erythrocyte glutathione reductase activation coefficient
(EGRac) (the gold-standard marker), but the functional significance of such findings is unclear since in general,
with the exception of younger women, dietary intakes of British and Irish adults are within dietary reference
ranges. Elsewhere in the world (including Canada and US), riboflavin biomarkers are not measured in nutrition
surveys because the existing biomarker EGRac requires very specific pre-analysis processing, unfeasible in most
settings. DERIVE will develop accessible riboflavin biomarkers in plasma (FMN, FAD, riboflavin) and identify
which most sensitively reflect dietary intakes and food sources of riboflavin in biobanked population based
cohorts from Ireland (n=1136) and Canada (n=1200), and are in best agreement with EGRac. The functional
significance of riboflavin will be evaluated using samples from previous placebo-controlled riboflavin
interventions (n=537; UK cohorts) to test whether correcting low status is associated with a functional response
related to a known metabolic role of riboflavin, the FMN-dependent generation of active vitamin B6 (i.e. PLP) in
tissues. Finally we will demonstrate an important health effect of riboflavin by investigating its role in modulating
blood pressure via a novel gene-nutrient interactive effect mediated through the FAD-dependent folate
metabolising enzyme methylenetetrahydrofolate reductase (MTHFR).

Technical Summary

Riboflavin (vitamin B2) is essential for oxidation-reduction reactions, but biomarker
status is rarely measured. The UK and Ireland are the only countries worldwide to have included a riboflavin
biomarker in national dietary surveys. Some concern exists in both countries regarding the large proportion of
adults showing low riboflavin status, measured using erythrocyte glutathione reductase activation coefficient
(EGRac) (the gold-standard marker), but the functional significance of such findings is unclear since in general,
with the exception of younger women, dietary intakes of British and Irish adults are within dietary reference
ranges. Elsewhere in the world (including Canada and US), riboflavin biomarkers are not measured in nutrition
surveys because the existing biomarker EGRac requires very specific pre-analysis processing, unfeasible in most
settings. DERIVE will develop accessible riboflavin biomarkers in plasma (FMN, FAD, riboflavin) and identify
which most sensitively reflect dietary intakes and food sources of riboflavin in biobanked population based
cohorts from Ireland (n=1136) and Canada (n=1200), and are in best agreement with EGRac. The functional
significance of riboflavin will be evaluated using samples from previous placebo-controlled riboflavin
interventions (n=537; UK cohorts) to test whether correcting low status is associated with a functional response
related to a known metabolic role of riboflavin, the FMN-dependent generation of active vitamin B6 (i.e. PLP) in
tissues. Finally we will demonstrate an important health effect of riboflavin by investigating its role in modulating
blood pressure via a novel gene-nutrient interactive effect mediated through the FAD-dependent folate
metabolising enzyme methylenetetrahydrofolate reductase (MTHFR).

Planned Impact

Our proposal also aligns with the overall vision of the JPI HDHL initiative that by 2030 all citizens will have accessibility to a healthy diet and that the incidence of non-communicable diseases will have decreased significantly. The project focusses on riboflavin, its interaction with two closely related vitamins - vitamin B6 and folate - and a related gene-nutrient interaction, with important implications for health. In the context of the overall diet, milk and dairy foods - the only rich sources of dietary riboflavin - will be of particular relevance.

Health Impact
The proposed research is well aligned with the goal of promoting health and wellness in adults in Europe and Associated countries. Recent evidence suggests that low riboflavin status may be a much greater public health issue globally than is generally recognised. There are considerable implications for those most at risk of low intake and status of riboflavin, including adults generally who do not consume adequate intakes of milk and dairy foods, and particularly young women of reproductive age. Of note, riboflavin has an important role in one-carbon metabolism where it interacts with other relevant nutrients (i.e. folate and vitamin B6) and is required for normal functioning of the essential pathways dependent on these nutrients. Thus better riboflavin status is likely to have important roles in maintaining health throughout the lifecycle and in preventing disease including cancer, age-related cataracts and metabolic disorders leading to increased risk of cardiovascular disease. The contribution of riboflavin to health is however very much under-recognised at this time, and the evidence greatly lacking, due to the lack of an accessible biomarker to measure riboflavin status. The proposed project will addresses this gap, by validating riboflavin biomarkers for use in the current proposal and in future nutrition research internationally.

Personalized nutrition: a novel role of riboflavin in health
The project will bring together cross-sectional data on blood pressure with genetic, nutrient, blood biomarker and relevant clinical information in well-characterized cohorts. This new knowledge will
provide important evidence to support a personalized nutritional strategy for preventing hypertension in genetically at-risk adults, with potential impacts on risk of disease in ageing (especially stroke),
pregnancy outcomes, healthcare economics, and quality of life throughout the lifecycle.

Societal and other impacts
Leadership within Europe and Associated countries. The proposal brings together researchers with complementary expertise to engage in diet and health research at a pan-European and
international level.
Improving scientific capacity and capability within Europe and Internationally: The proposed project will establish a critical mass of expertise in this research area, allowing for excellent
science and sharing of innovative approaches and infrastructures, and the data gathered willbe made available to the wider research community. It will also offer excellent experience and
training to early career scientists and PhD students in nutrition, and thus contribute to strengthening the research base in the health and agri-food sectors.
The scientific publications arising from this research will also provide important benefits to the wider research community by providing comprehensive data reporting important nutrition and
health relationships in very well characterised observational cohorts, along with evidence of the potential benefits of improving micronutrient status for better health outcomes as demonstrated
in intervention cohorts.
Collaboration with industry and Enhanced competitiveness in the Agri-food sector: The new biomarkers of dietary riboflavin intake validated in the current project will be essential for substantiating Article 14 health claims for evaluation by the European Food Safety Authority.

Publications

10 25 50
 
Description Early findings from the DERiVE Project were presented at the 13th European Nutrition Conference of the Federation of European Nutrition Societies (FENS) 2019 conference in Dublin, Ireland. The conference took place from 15 - 18 October 2019 in the Convention Centre Dublin, Ireland. The early results from the DERiVE Project were presented by Prof Mary Ward and Prof Yvonne Lamers at the DERiVE-VALID Symposium 'Validating novel biomarkers of dietary exposure to Riboflavin and Polyphenols and associated health impacts'.

Prof Lamers described newly validated methods for quantifying riboflavin status in human plasma that have been developed using liquid chromatography - tandem mass spectrometry (LC-MS/MS) as part of the DERiVE project. The validity of these methods were tested in samples previously collected from adult cohorts in the UK, Ireland and Canada. In addition, the sensitivity of these new methods is on-going and will be determined by assessing the response to riboflavin intervention at different doses.

Further achievements by the DERiVE project include increased research capabilities through the training of PhD students and early career researchers, namely training in laboratory techniques required for the project, technology transfer between collaborating research groups, experience in the transfer of human samples and training in public speaking received at the JPI HDHL international conference.
Exploitation Route Newly validated riboflavin biomarkers have been developed through the DERiVE project and will provide accessible high-throughput biomarkers for use in population based studies and RCTs, will enable further understanding of the role of riboflavin status in promoting health and preventing disease across the globe.

This project can contribute to the area of personalised nutrition using a novel role of riboflavin in health. The DERiVE project brought together data from three international cohorts (from the UK, Ireland and Canada) and analysed blood pressure in relation to genetic, nutrient, blood biomarker and relevant clinical information in these well-characterised cohorts. This new knowledge will provide important evidence to support a personalised nutritional strategy for preventing hypertension in genetically at-risk adults, with potential impacts on risk of disease in ageing (especially stroke), pregnancy outcomes, healthcare economics, and quality of life throughout the lifecycle.

The newly validated biomarkers of dietary riboflavin intake, in the current project, will be essential for substantiating Article 14 health claims for evaluation by the European Food Safety Authority. This will in turn be required for developing novel functional foods enhanced with riboflavin designed to meet the needs of those consumers who cannot, or choose not to, consume milk and dairy foods (the only rich natural sources of riboflavin).

The scientific publications arising from this research will also provide important benefits to the wider research community by providing comprehensive data reporting important nutrition and health relationships in very well characterised observational cohorts, along with evidence of the potential benefits of improving micronutrient status for better health outcomes as demonstrated in intervention cohorts.
Sectors Agriculture, Food and Drink,Education,Healthcare,Pharmaceuticals and Medical Biotechnology

URL http://derive-riboflavin.com/research-project
 
Description Early findings from the DERiVE Project were presented at the 13th European Nutrition Conference of the Federation of European Nutrition Societies (FENS) 2019 conference in Dublin, Ireland. The conference took place from 15 - 18 October 2019 in the Convention Centre Dublin, Ireland. Prof Mary Ward and Prof Yvonne Lamers represented the DERiVE team at the DERiVE-VALID Symposium 'Validating novel biomarkers of dietary exposure to Riboflavin and Polyphenols and associated health impacts'. This symposium was a unique opportunity to highlight and disseminate results from the DERiVE project. Arising from the transnational collaboration of the DERiVE project (Canada and the UK), further funding has been awarded from JPI ERA-HDHL JFA2 "Nutrition & the Epigenome" scheme for the EpiBrain Project: Epigenetic effects of B-vitamins on brain health throughout life: scientific substantiation and translation of evidence for health-improvement strategies. EpiBrain project coordinator: Prof Yvonne Lamers, UBC, Canada. Impact article on nutritional biomarkers for health 'Polyphenols for healthier ageing' Authors: Ward, Mary; McNulty, Helene Source: Impact, Volume 2018, Number 10, December 2018, pp. 83-85(3) Publisher: Science Impact Ltd DOI: https://doi.org/10.21820/23987073.2018.10.83 Impact article targeted at international research & scientific community, stakeholders & policymakers. The article was produced to create awareness of the DERIVE project and outline the impact objectives: *Gain a better understanding of the role of nutrition in health by developing and validating novel biomarkers of dietary exposure in transnational research studies *Grow capacity, capability and sustainable transnational partnerships in nutrition and health research, led by UK partner in collaboration with partners in the Republic of Ireland and Canada *Demonstrate the functional and metabolic effects of optimal riboflavin status on cardiovascular health. The DERIVE project is funded under the JPI co-funded ERA-HDHL joint call 'Biomarker for nutrition and health'. In line with the JPI's 'A healthy diet for a healthy life' vision to support quality, transnational, collaborative research, the goal of this project is to promote scientific excellence through international collaborations. Go to www.healthydietforhealthylife.eu/index.php/joint-actions/era-hdhl-ja
Sector Agriculture, Food and Drink,Education,Healthcare,Pharmaceuticals and Medical Biotechnology
Impact Types Societal,Policy & public services

 
Description Citation in DSM Whitepaper on 'The role of riboflavin in resistant hypertension'
Geographic Reach Multiple continents/international 
Policy Influence Type Citation in clinical reviews
Impact Research from Ulster University, in relation to this project, was included as a citation in a DSM Whitepaper on 'The role of riboflavin in resistant hypertension', published in December 2019.
URL https://www.dsm.com/markets/pharma/en/education/whitepapers.html
 
Description Comment provided for EFSA dietary reference values for riboflavin
Geographic Reach Europe 
Policy Influence Type Gave evidence to a government review
URL https://efsa.onlinelibrary.wiley.com/doi/epdf/10.2903/sp.efsa.2017.EN-1268
 
Description Prof Kristina Pentieva: Member of the Working Group on Dietary Reference Values for vitamins (Riboflavin)
Geographic Reach Europe 
Policy Influence Type Membership of a guideline committee
URL https://efsa.onlinelibrary.wiley.com/doi/epdf/10.2903/sp.efsa.2017.EN-1268
 
Description Prof Mary Ward; Deputy Director of The Wellcome Trust-Wolfson Northern Ireland Clinical Research Facility (NICRF)
Geographic Reach National 
Policy Influence Type Membership of a guideline committee
URL https://www.qub.ac.uk/research-centres/TheWellcomeTrust-WolfsonNorthernIrelandClinicalResearchFacili...
 
Description HDHL EpiBrain: Epigenetic effects of B-vitamins on brain health throughout life
Amount £266,880 (GBP)
Funding ID BB/S020330/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 03/2019 
End 02/2022
 
Description OptiPreg
Amount £164,850 (GBP)
Organisation DSM 
Sector Private
Country Netherlands
Start 02/2018 
 
Title Altering sample volume and concentration of reagents on the EGRac assay results 
Description We aimed to alter volume / concentrations of sample and reagents in order to achieve stronger signal on our new DAYTONA+ clinical analyser. 
Type Of Material Biological samples 
Year Produced 2019 
Provided To Others? No  
Impact The improved version of the method gives analytical results identical to those from the previous version, but the new method is more robust because it is not susceptible to noise that arises from small fluctuations in machine performance. 
 
Title Retrospectively washing red blood cells after preparation and results of the EGRac assay 
Description Erythrocytes from the BC Generations project are frozen without the thrice-washing step which is a usual part of our sample preparation. We sought to determine whether it is possible to apply this step retrospectively, to thawed samples. 
Type Of Material Biological samples 
Year Produced 2019 
Provided To Others? No  
Impact We concluded that it is not possible to wash freeze-thawed erythrocytes because they are subject to some lysis during the freeze-thaw. 
 
Title The impact of different methods of sample preparation on the EGRac assay results 
Description Since it was impossible to retrospectively wash freeze-thawed erythrocytes, we sought instead to assess the effect of removing this step, which is a laborious part of sample processing implemented prior to the EGRac assay. We prepared 2 samples from each participant, 1. Samples processed routinely (thrice washing) and 2. Unwashed erythrocytes. The EGRac values resulting from the 2 separate procedures were then compared, to determine the level of agreement. 
Type Of Material Biological samples 
Year Produced 2019 
Provided To Others? No  
Impact The results from our experiment indicated that although unwashed samples did result in slightly lower EGRac ratios (better riboflavin status), overall, the washed and unwashed erythrocytes provided an acceptable level of agreement in regards to the resulting EGRac values. 
 
Description Randox 
Organisation Randox Laboratories
Country Global 
Sector Private 
PI Contribution Daytona+ analyser for biomarker analysis of riboflavin status (EGRac)
Collaborator Contribution Daytona+ analyser for biomarker analysis of riboflavin status (EGRac)
Impact N/A
Start Year 2017
 
Description The DERIVE Project 
Organisation DSM
Department DSM Nutritional Products
Country Switzerland 
Sector Private 
PI Contribution Ulster is responsible for the overall co-ordination of the projected and for contributing samples from a series of randomised controlled riboflavin intervention trials in healthy and CVD cohorts to facilitate the development and validation of new riboflavin biomarkers by the Canadian partner. Ulster were the first to report the role of a riboflavin as a modulator of blood pressure in Northern Irish adults with a genetic pre-disposition to hypertension. To date this association has not been investigated in other population cohorts thus the DERIVE project will address this question. As overall co-ordinators Ulster are responsible for arranging regular partner meetings via Skype / Tele-call and face-to-face where possible and recording and ensuring delivery of actions arising from such meetings.
Collaborator Contribution Canadian partners will be responsible for developing more accessible riboflavin biomarkers to enable a more comprehensive picture of riboflavin status globally to be reported. This will be achieved using a newly developed high-throughput automated Mass Spec method. The validity and sensitivity of the method will be established using RCT samples from Ulster (UK partner). In addition riboflavin status (as measured by the new Mass Spec method) will be determined in Irish and Canadian population cohorts. The samples from the Irish population cohort have previously been analysed for riboflavin status using the gold-standard semi-functional method EGRac and will be made available for comparison. Irish partners will also contribute significant expertise in relation to the collection and analysis of accurate dietary information.
Impact No outputs yet
Start Year 2017
 
Description The DERIVE Project 
Organisation University College Cork
Country Ireland 
Sector Academic/University 
PI Contribution Ulster is responsible for the overall co-ordination of the projected and for contributing samples from a series of randomised controlled riboflavin intervention trials in healthy and CVD cohorts to facilitate the development and validation of new riboflavin biomarkers by the Canadian partner. Ulster were the first to report the role of a riboflavin as a modulator of blood pressure in Northern Irish adults with a genetic pre-disposition to hypertension. To date this association has not been investigated in other population cohorts thus the DERIVE project will address this question. As overall co-ordinators Ulster are responsible for arranging regular partner meetings via Skype / Tele-call and face-to-face where possible and recording and ensuring delivery of actions arising from such meetings.
Collaborator Contribution Canadian partners will be responsible for developing more accessible riboflavin biomarkers to enable a more comprehensive picture of riboflavin status globally to be reported. This will be achieved using a newly developed high-throughput automated Mass Spec method. The validity and sensitivity of the method will be established using RCT samples from Ulster (UK partner). In addition riboflavin status (as measured by the new Mass Spec method) will be determined in Irish and Canadian population cohorts. The samples from the Irish population cohort have previously been analysed for riboflavin status using the gold-standard semi-functional method EGRac and will be made available for comparison. Irish partners will also contribute significant expertise in relation to the collection and analysis of accurate dietary information.
Impact No outputs yet
Start Year 2017
 
Description The DERIVE Project 
Organisation University of British Columbia
Country Canada 
Sector Academic/University 
PI Contribution Ulster is responsible for the overall co-ordination of the projected and for contributing samples from a series of randomised controlled riboflavin intervention trials in healthy and CVD cohorts to facilitate the development and validation of new riboflavin biomarkers by the Canadian partner. Ulster were the first to report the role of a riboflavin as a modulator of blood pressure in Northern Irish adults with a genetic pre-disposition to hypertension. To date this association has not been investigated in other population cohorts thus the DERIVE project will address this question. As overall co-ordinators Ulster are responsible for arranging regular partner meetings via Skype / Tele-call and face-to-face where possible and recording and ensuring delivery of actions arising from such meetings.
Collaborator Contribution Canadian partners will be responsible for developing more accessible riboflavin biomarkers to enable a more comprehensive picture of riboflavin status globally to be reported. This will be achieved using a newly developed high-throughput automated Mass Spec method. The validity and sensitivity of the method will be established using RCT samples from Ulster (UK partner). In addition riboflavin status (as measured by the new Mass Spec method) will be determined in Irish and Canadian population cohorts. The samples from the Irish population cohort have previously been analysed for riboflavin status using the gold-standard semi-functional method EGRac and will be made available for comparison. Irish partners will also contribute significant expertise in relation to the collection and analysis of accurate dietary information.
Impact No outputs yet
Start Year 2017
 
Description The VALID Project 
Organisation Trinity College Dublin
Country Ireland 
Sector Academic/University 
PI Contribution Valerolactones and healthy Ageing: LInking Dietary factors, nutrient biomarkers, metabolic status and inflammation with cognition in older adults
Collaborator Contribution The VALID project is a sister project of DERiVE. They were both awarded under the ERA-HDHL Joint Transnational Research Proposal: "Biomarkers for Nutrition and Health" and funded by the BBSRC. The VALID project will validate novel plasma biomarkers of procyanidin-rich foods and link them with inflammation, metabolic health and cognition in an ageing European population. Polyphenols, particularly procyanidins (abundant in foods such as tea, cocoa, grapes, nuts and berries), may be beneficial in maintaining better cognitive function in ageing, but investigating their role in relation to health is hampered by the lack of robust biomarkers of dietary intake. VALID draws on a unique cohort of 5200 adults aged 60-102 years recruited from the UK and Ireland, providing a range of biomarkers and health measures. Apart from performing new analysis on bio-banked TUDA samples, the VALID project will access 'TUDA 5+', a follow-up study of 1000 participants from the original cohort 5 years after initial investigation, to determine the role of procyanidin-rich foods in preventing cognitive decline over a 5-year follow-up period.
Impact IMPACT article: DERIVE - Development of Riboflavin biomarkers to relate dietary sources with status, gene-nutrient Interactions and Validated health Effects in adult cohorts - & VALID - Valerolactones and healthy Ageing: LInking Dietary factors, nutrient biomarkers, metabolic status and inflammation with cognition in older adults DOI:10.21820/23987073.2018.10.83 Federation of European Nutrition Societies (FENS) 13th European Nutrition Conference; DERIVE-VALID Symposium (S2-2C-3) Validating novel biomarkers of dietary exposure to Riboflavin and Polyphenols and associated health impacts: Transnational DERiVE and VALID projects. Wednesday 16th October 2019 15:00-16:30; Wicklow Meeting Room 1 (Level 2)
Start Year 2017
 
Description The VALID Project 
Organisation Ulster University
Country United Kingdom 
Sector Academic/University 
PI Contribution Valerolactones and healthy Ageing: LInking Dietary factors, nutrient biomarkers, metabolic status and inflammation with cognition in older adults
Collaborator Contribution The VALID project is a sister project of DERiVE. They were both awarded under the ERA-HDHL Joint Transnational Research Proposal: "Biomarkers for Nutrition and Health" and funded by the BBSRC. The VALID project will validate novel plasma biomarkers of procyanidin-rich foods and link them with inflammation, metabolic health and cognition in an ageing European population. Polyphenols, particularly procyanidins (abundant in foods such as tea, cocoa, grapes, nuts and berries), may be beneficial in maintaining better cognitive function in ageing, but investigating their role in relation to health is hampered by the lack of robust biomarkers of dietary intake. VALID draws on a unique cohort of 5200 adults aged 60-102 years recruited from the UK and Ireland, providing a range of biomarkers and health measures. Apart from performing new analysis on bio-banked TUDA samples, the VALID project will access 'TUDA 5+', a follow-up study of 1000 participants from the original cohort 5 years after initial investigation, to determine the role of procyanidin-rich foods in preventing cognitive decline over a 5-year follow-up period.
Impact IMPACT article: DERIVE - Development of Riboflavin biomarkers to relate dietary sources with status, gene-nutrient Interactions and Validated health Effects in adult cohorts - & VALID - Valerolactones and healthy Ageing: LInking Dietary factors, nutrient biomarkers, metabolic status and inflammation with cognition in older adults DOI:10.21820/23987073.2018.10.83 Federation of European Nutrition Societies (FENS) 13th European Nutrition Conference; DERIVE-VALID Symposium (S2-2C-3) Validating novel biomarkers of dietary exposure to Riboflavin and Polyphenols and associated health impacts: Transnational DERiVE and VALID projects. Wednesday 16th October 2019 15:00-16:30; Wicklow Meeting Room 1 (Level 2)
Start Year 2017
 
Description The VALID Project 
Organisation University of Parma
Country Italy 
Sector Academic/University 
PI Contribution Valerolactones and healthy Ageing: LInking Dietary factors, nutrient biomarkers, metabolic status and inflammation with cognition in older adults
Collaborator Contribution The VALID project is a sister project of DERiVE. They were both awarded under the ERA-HDHL Joint Transnational Research Proposal: "Biomarkers for Nutrition and Health" and funded by the BBSRC. The VALID project will validate novel plasma biomarkers of procyanidin-rich foods and link them with inflammation, metabolic health and cognition in an ageing European population. Polyphenols, particularly procyanidins (abundant in foods such as tea, cocoa, grapes, nuts and berries), may be beneficial in maintaining better cognitive function in ageing, but investigating their role in relation to health is hampered by the lack of robust biomarkers of dietary intake. VALID draws on a unique cohort of 5200 adults aged 60-102 years recruited from the UK and Ireland, providing a range of biomarkers and health measures. Apart from performing new analysis on bio-banked TUDA samples, the VALID project will access 'TUDA 5+', a follow-up study of 1000 participants from the original cohort 5 years after initial investigation, to determine the role of procyanidin-rich foods in preventing cognitive decline over a 5-year follow-up period.
Impact IMPACT article: DERIVE - Development of Riboflavin biomarkers to relate dietary sources with status, gene-nutrient Interactions and Validated health Effects in adult cohorts - & VALID - Valerolactones and healthy Ageing: LInking Dietary factors, nutrient biomarkers, metabolic status and inflammation with cognition in older adults DOI:10.21820/23987073.2018.10.83 Federation of European Nutrition Societies (FENS) 13th European Nutrition Conference; DERIVE-VALID Symposium (S2-2C-3) Validating novel biomarkers of dietary exposure to Riboflavin and Polyphenols and associated health impacts: Transnational DERiVE and VALID projects. Wednesday 16th October 2019 15:00-16:30; Wicklow Meeting Room 1 (Level 2)
Start Year 2017
 
Description University of Exeter 
Organisation University of Exeter
Country United Kingdom 
Sector Academic/University 
PI Contribution UU student training on vascular techniques and nitric oxide analysis for mechanisms underpinning gene-nutrient interaction in BP.
Collaborator Contribution UU student training on vascular techniques and nitric oxide analysis for mechanisms underpinning gene-nutrient interaction in BP.
Impact N/A
Start Year 2019
 
Description Waters 
Organisation Waters Corporation
Department Waters Corporation
Country United Kingdom 
Sector Private 
PI Contribution HPLC equipment and methodology for biomarker analysis of vitamin B6
Collaborator Contribution HPLC equipment and methodology for biomarker analysis of vitamin B6
Impact N/A
Start Year 2017
 
Description 5th International Vitamin Conference, Sydney, Australia: Relationship between riboflavin MTHFR and hypertension. Invited talk by Prof Helene McNulty 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Vitamins and Wellbeing from Tomb to Womb: Relationship between riboflavin MTHFR and hypertension: Opportunity to highlight the role of this novel gene-nutrient interaction to an international audience, including representatives from Pharma
Year(s) Of Engagement Activity 2018
 
Description ERA-HDL: Biomarkers in Nutrition and Health, The Hague, Netherlands July 2018. DERIVE update by co-investigator Prof JJ Strain 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Project update to relevant stakeholders at the ERA-HDHL: Biomarkers in Nutrition and Health conference: The Hague, 4-5th July by Professor Sean Strain
Year(s) Of Engagement Activity 2018
 
Description A Novel role for Dairy in Healthy Blood Pressure: up close and personal; World Dairy Summit satellite event, Ulster University, Coleraine 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact A delegate of international representatives from the global Dairy Industry attended this workshop to hear about the studies we have conduced demonstrating a role for riboflavin as a targeted treatment for hypertension in genetically at-risk adults. The importance of dairy as a source of riboflavin in our diet was discussed and the challenges of estimating riboflavin status in global populations was highlighted, some of which we hope to address in the DERIVE project.
Year(s) Of Engagement Activity 2017
 
Description Amy Tan (UBC): FENS 2019 conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Amy Tan (Prof Lamers' Team in collaboration with Prof McNulty and Prof Ward) 'Plasma riboflavin concentration as novel indicator for vitamin-B2 status assessment: suggested cutoffs and its association with vitamin-B6 status in women' Poster presentation at the Federation of European Nutrition Societies (FENS) 2019, 13th European Nutrition Conference, Dublin, Ireland; 15-18 October 2019
Year(s) Of Engagement Activity 2019
 
Description Association of key sources of riboflavin with EGRac status in a representative sample of Irish adults aged 18-90 years. Laura Kehoe, DERiVE PhD researcher, UCC 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Opportunity to disseminate findings at annual Nutrition society winter meeting
Year(s) Of Engagement Activity 2018
 
Description Blood pressure and hypertension in relation to cognitive performance in older Irish adults from the TUDA cohort: Preliminary analysis. Harry Jarrett, DERiVE PhD researcher, Ulster 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Opportunity to highlight the role of a novel gene-nutrient interaction and its role in blood pressure to a specialist audience of experts in the area of Hypertension.
Year(s) Of Engagement Activity 2018
URL https://www.nature.com/articles/s41371-018-0109-3
 
Description Blood pressure update for RIBOGENE participants 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Study participants or study members
Results and Impact 90 adults (participants and their family/friends) attended one of three public events held at venues across Northern Ireland (Belfast, Coleraine and Derry) to receive an update on research being conducted at the Nutrition Innovation Centre for Food and Health investigating the association between B-vitamins, blood pressure and a common genetic risk factor affecting 12% of the population. The event included 2 short presentations followed by a lively question and answer session. All those who attended provided very positive feedback following the event.
Year(s) Of Engagement Activity 2018
URL https://twitter.com/NICHE_ULSTER/status/956298382703816706
 
Description DERIVE project elevator pitch by Harry Jarrett, PhD researcher on DERIVE. Joint Programming Initiative "A Healthy Diet for a Healthy Life" (JPI HDHL) workshop 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact Harry Jarrett, PhD researcher was invited to give a 'pitch' on the DERIVE project and received public speaking 'Speak like Obama' training. This provided an opportunity to highlight the need to develop accessible riboflavin biomarkers for use in global population cohorts.
Year(s) Of Engagement Activity 2017
URL https://www.youtube.com/watch?v=C00DjajiSiI
 
Description DERiVE Twitter account 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact https://twitter.com/derive_1
Year(s) Of Engagement Activity 2018,2019,2020
 
Description DERiVE project webpage 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Webpage providing information regarding this Transnational collaborative project as well as latest updates / publications.
Year(s) Of Engagement Activity 2018,2019,2020
URL http://derive-riboflavin.com/research-project
 
Description DSM symposium, Switzerland: Role of Nutrition to Support Health throughout the Lifespan: How to translate science into public health interventions at critical stages of the lifespan, by Prof Helene McNulty, Ulster 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact DSM Special symposium in Kaisergaust, Switzerland. Prof Helene McNulty Invited Speaker: Role of Nutrition to Support Health throughout the Lifespan: How to translate science into public health interventions at critical stages of the lifespan.

Opportunity to engage with Industry and present latest developments arising from the ongoing research
Year(s) Of Engagement Activity 2018
 
Description Dr Emma O'Sullivan (UCC): FENS 2019 conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dr Emma O'Sullivan 'Intakes and status of riboflavin in a representative sample of Irish adults aged 18-90 years screened for MTHFR C677T polymorphism' Federation of European Nutrition Societies 13th European Nutrition Conference, Dublin, Ireland; 15-18 October 2019
Year(s) Of Engagement Activity 2019
 
Description Dr Laura Kehoe (UCC): FENS 2019 conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dr Laura Kehoe 'The role of fortified foods and nutritional supplements in the diets of older Irish adults' Federation of European Nutrition Societies 13th European Nutrition Conference, Dublin, Ireland; 15-18 October 2019
Year(s) Of Engagement Activity 2019
 
Description Dr Laura Kehoe (UCC): FENS 2019 conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dr Laura Kehoe 'Nutritional challenges for older adults in Europe: current status and future directions' Federation of European Nutrition Societies 13th European Nutrition Conference, Dublin, Ireland; 15-18 October 2019
Year(s) Of Engagement Activity 2019
 
Description Dr Liadhan McAnena - poster presentation at the 12th International Conference on One Carbon Metabolism, B Vitamins and Homocysteine, Spain, 9-13 June 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dr Liadhan McAnena 'Is riboflavin deficiency in women of reproductive age a global problem?' 12th International Conference on One Carbon Metabolism, B Vitamins and Homocysteine: Understanding their role in health and disease from the womb to old age, Southern Catalonia, Spain 9th-13th June 2019
Year(s) Of Engagement Activity 2019
 
Description Dr Liadhan McAnena: presentation at DSM-Ulster Symposium, Ulster University, Coleraine, 20 May 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Dr Liadhan McAnena 'Is riboflavin deficiency in women of reproductive age a global problem?' DSM-Ulster Symposium, Coleraine, Northern Ireland, 20th May 2019
Year(s) Of Engagement Activity 2019
 
Description FASEB, Nova Scotia 2018: Riboflavin status, MTHFR and blood pressure. Invited talk by DERiVE co-investigator Prof Helene McNulty: 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Federation of American Societies for Experimental Biology (FASEB) Conference, Nova Scotia, Canada
Invited talk by Professor Helene McNulty: Riboflavin status and MTHFR and blood pressure
Year(s) Of Engagement Activity 2018
 
Description FASEB. Novia Scotia, August 2018; Riboflavin, vitamin B6, and related metabolites detected in plasma in a single LC-MS/MS platform Nadia Moran-Garcia, DERIVE post-doctoral researcher. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact An opportunity to discuss early findings from the newly developed method to assess riboflavin status with international experts and to highlight the DERiVE project.
Year(s) Of Engagement Activity 2018
 
Description Impact article on nutritional biomarkers for health 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Impact article targeted at international research & scientific community, stakeholders & policymakers. The article was produced to create awareness of the VALID project and outline the impact objectives:
*Gain a better understanding of the role of nutrition in health by developing and validating novel biomarkers of dietary exposure in transnational research studies
*Grow capacity, capability and sustainable transnational partnerships in nutrition and health research, led by UK partner in collaboration with partners in the Republic of Ireland and Italy.
*Demonstrate the functional and metabolic effects of dietary polyphenols on the ageing brain

The VALID project is funded under the JPI co-funded ERA-HDHL joint call 'Biomarker for nutrition and health'. In line with the JPI's 'A healthy diet for a healthy life' vision to support quality, transnational, collaborative research, the goal of this project is to promote scientific excellence through international collaborations. Go to www.healthydietforhealthylife.eu/index.php/joint-actions/era-hdhl-ja

Published in Science Impact, December 2018, pp. 83-85; https://doi.org/10.21820/23987073.2018.10.83
Year(s) Of Engagement Activity 2018
URL https://doi.org/10.21820/23987073.2018.10.83
 
Description Intake, status and dietary sources of riboflavin in a representative sample of Irish adults aged 18-90 years. Laura Kehoe, DERiVE PhD researcher, UCC 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Opportunity to disseminate findings at the 2018 Nutrition Society Summer Meeting in a poster presentation.
Year(s) Of Engagement Activity 2018
 
Description Irish Nutrition Society conference 2018: Approaches to improving micronutrient status assessment at a population level by Prof Yvonne Lamers, UBC 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited speaker at Nutrition Society (Irish Section) Conference 2018: Targeted approaches to tackling current nutritional issues. 20-22nd June 2018
Year(s) Of Engagement Activity 2017,2018
URL https://www.nutritionsociety.org/node/392/programme/day-two
 
Description Larisse Melo (UBC): FENS 2019 conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Larisse Melo (Prof Lamers' Team) 'Development of novel vitamin B12 fortified yogurts using isolated and microencapsulated vitamin B12', Poster presentation at the Federation of European Nutrition Societies (FENS) 2019, 13th European Nutrition Conference, Dublin, Ireland; 15-18 October 2019
Year(s) Of Engagement Activity 2019
 
Description Mr Harry Jarrett: presentation at B-vitamin research group workshop, Ulster University, 18-19th November 2019 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Mr Harry Jarrett (PhD Researcher) 'The metabolic interaction between riboflavin & vitamin B6 in European adults'. Dr Amanda McFarlane and B-vitamin Research group workshop, Coleraine, Northern Ireland, 18-19th November 2019
Year(s) Of Engagement Activity 2019
 
Description Mr Harry Jarrett: presentation at The Nutrition Society Irish Section Postgraduate Conference, Dublin, 12th - 14th February 2020 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Mr Harry Jarrett (PhD Researcher) 'The metabolic interaction between riboflavin & vitamin B6 in European adults'. The Nutrition Society Irish Section Postgraduate Conference, Dublin, 12th - 14th February 2020
Year(s) Of Engagement Activity 2020
 
Description Nutrition Society conference 2018: Folate and related B vitamins through the lifecyle: public health priorities and challenges by Prof Helene McNulty, Ulster University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Irish Nutrition Society (Irish Section) Conference 2018: Targeted approaches to tackling current nutritional issues. 20-22nd June 2018
Year(s) Of Engagement Activity 2018
URL https://www.nutritionsociety.org/node/392/programme
 
Description Presentation of DERIVE scientific video log at the 5th international l JPI Healthy Diet for a Healthy Life conference by Dr Nadia Moran-Garcia 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Video log to communicate the nature of the transnational collaboration and study objectives
Year(s) Of Engagement Activity 2019
URL https://www.youtube.com/watch?v=YMPmjX4oh4o
 
Description Prof Albert Flynn & Dr Janette Walton (UCC): National Children's Food Survey II: Launch of summary findings' Dublin, Ireland 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Prof Albert Flynn & Dr Janette Walton presented 'National Children's Food Survey II: Launch of summary findings' Dublin, Ireland 11 September 2019
Year(s) Of Engagement Activity 2019
 
Description Prof Helene McNulty: Co-chair of the Executive Committee, 13th FENS European Nutrition Conference, Dublin, Oct 2019 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Prof Helene McNulty was co-chair of the Executive Committee, Federation of European Nutrition Societies 13th European Nutrition Conference, Dublin, Ireland; 15-18 October 2019.
Year(s) Of Engagement Activity 2019
URL http://www.fens2019.org/welcome/letter-from-co-chairs/
 
Description Prof Helene McNulty: presentation at the EuroPrevent Congress, European Association of Preventative Cardiology, Ljubljana, Slovenia, April 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited speaker presentation at the EuroPrevent Congress, European Association of Preventative Cardiology, Ljubljana, Slovenia, 19 April 2018.
Presentation: 'Role of riboflavin in reducing blood pressure in genetically at-risk adults'
Year(s) Of Engagement Activity 2018
URL http://spo.escardio.org/
 
Description Prof Helene McNulty: presentation at the Nutrition Society Irish Section conference, Coleraine, Northern Ireland, June 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited speaker presentation at the Nutrition Society Irish Section conference - Tackling Current Nutritional Issues, Coleraine, Northern Ireland, 20 June 2018
Presentation: 'Folate and related B-vitamins through the lifecycle - public health priorities and challenges'
Year(s) Of Engagement Activity 2018
URL https://www.nutritionsociety.org/events/irish-section-conference-2018-targeted-approaches-tackling-c...
 
Description Prof Mary Ward and Prof Yvonne Lamers: presentation at the DERiVE-VALID Symposium at the FENS 2019 conference 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact At the DERiVE-VALID Symposium, FENS conference, Dublin, Ireland, Prof Mary Ward (symposium chairpersion) presented "The metabolic interaction of the B-vitamins riboflavin and vitamin B6 within one-carbon metabolism in European adult cohorts - The DERiVE Project". Furthermore, Prof Yvonne Lamers presented "Validation of biomarkers to assess riboflavin status in Irish and Canadian population cohorts: The DERiVE Project".
The symposium was well received with great participation from the FENS conference scientific audience.
Year(s) Of Engagement Activity 2019
 
Description Prof Mary Ward: presentation at The Dairy Council for NI 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Prof Mary Ward 'Dairy foods and blood pressure: a review of current evidence', The Dairy Council for NI - Nutrition & Health What's New Conference, Belfast, Northern Ireland, April 2019
Year(s) Of Engagement Activity 2019
 
Description Prof Mary Ward; Deputy Director of The Wellcome Trust-Wolfson Northern Ireland Clinical Research Facility (NICRF) 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Strategic role with the aim of facilitating research partnerships and collaborative between Ulster University, Queens University Belfast and The Belfast Trust. This also involves working closely with a range of other stakeholders including service users.
Year(s) Of Engagement Activity 2017,2018,2019
URL https://www.qub.ac.uk/research-centres/TheWellcomeTrust-WolfsonNorthernIrelandClinicalResearchFacili...
 
Description Profs Mary Ward and JJ Strain: Members of the Organising Committee at the FENS 2019 conference 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Numerous members of the team played key roles in the organisation of the FENS 2019 conference: Prof Mary Ward, Prof JJ Strain were members of the Organising Committee.
Year(s) Of Engagement Activity 2019
 
Description Riboflavin (Vitamin B2): a novel treatment for Hypertension in - Latest trends of vitamins and lipids in therapeutic applications. Frankfurt, October 2017 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact This presentation to a Pharma audience highlighted the potential for riboflavin to modulate blood pressure in adults with a specific genetic risk factor for hypertension, affecting up to 32% of some populations globally. The audience consisted of approximately 100 representatives from the Pharma industry who were in Frankfurt to attend a major European Pharma conference. The event was organised by DSM..
Year(s) Of Engagement Activity 2017
 
Description The role of riboflavin in reducing blood pressure in genetically atrisk hypertensive adults by Prof Helene McNulty at EuroPrevent 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Opportunity to present to policymakers and healthcare professionals as well as patient groups on role of a novel gene-nutrient interaction in blood presssure.. EuroPrevent Satellite Symposium, Ljubljana, Slovenia. 19 April 2018
Year(s) Of Engagement Activity 2018