AUTOMATIC CONTROL OF MAMMALIAN GENE EXPRESSION USING A MICROFLUIDIC PLATFORM

Lead Research Organisation: University of Bristol
Department Name: Engineering Mathematics

Abstract

ITALY
ITALY

Publications

10 25 50
 
Description This funding allowed getting relevant training in mammalian cell external control, which resulted in the generation of data about the control of stem cells using microfluidics devices. The objectives of the grant were met; this award facilitated preparation of further grant proposals, and results obtained with the platform we set-up have been recently published.
Exploitation Route The RAs in my group, thanks to the training received, have set-up a microfluidics/microscopy platform in Bristol. My group is currently performing experiments using the platform, and other researchers at Bristol University have been also trained to use it.
Sectors Education,Pharmaceuticals and Medical Biotechnology

 
Description The platform we set-up, thanks to training received through this award, was used in public engagement activities facilitated by Bristol University.
First Year Of Impact 2018
Sector Education
Impact Types Cultural

 
Description Engineering for a prosperous nation
Amount £250,704 (GBP)
Funding ID EP/R041695/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Public
Country United Kingdom
Start 02/2018 
End 05/2019
 
Description Horizon 2020 FET-Open action "Control Engineering of Biological Systems for Reliable Synthetic Biology Applications"
Amount € 3,000,000 (EUR)
Funding ID Grant agreement ID: 766840 
Organisation European Commission 
Department Horizon 2020
Sector Public
Country European Union (EU)
Start 10/2017 
End 09/2020
 
Title Automatic feedback control of gene expression in mammalian cells 
Description We developed a microfluidics/microscopy platform to measure and control gene expression in mammalian cells. This, in turns, required specialised software for online cell segmentation, control and actuation. 
Type Of Material Model of mechanisms or symptoms - in vitro 
Year Produced 2018 
Provided To Others? Yes  
Impact The methodology can be used to track dynamics in living mammalian cells, and to automatically infer improved protocols. Results published in Postiglione et al, ACS Synth. Biol. 2018, 7, 11, 2558-2565. 
URL https://pubs.acs.org/doi/abs/10.1021/acssynbio.8b00235
 
Description Collaboration on mammalian cell control 
Organisation Telethon Foundation
Department Telethon Institute of Genetics and Medicine (TIGEM)
Country Italy 
Sector Charity/Non Profit 
PI Contribution My team has contributed to this collaboration by generating preliminary feedback control experiments in mouse embryonic stem cells, using a microfluidics device, an actuation platform and control algorithms.
Collaborator Contribution The partner has provided segmentation/control algorithm code, and hosted 2 RAs in my group in his lab for 10 days.
Impact The collaboration is multidisciplinary (e.g. involves combination of experimental work, and modelling/programming work). Results from this collaboration are included in a journal paper, currently in preparation.
Start Year 2017
 
Description Open Days Engineering, Bristol University (UK) 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact Open Days for A level students, which involved presentations by PhD students and PDRAs, and demos.
Year(s) Of Engagement Activity 2017,2018,2019,2020
 
Description Oral Presentation, Research Conference, 18th European Control Conference (ECC) 2019, Naples (Italy). 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Interdisciplinary conference about principles and applications of control engineering.
Year(s) Of Engagement Activity 2019
URL https://ecc19.eu/
 
Description Oral Presentation, Research Conference, Foundations of Systems Biology in Engineering (FOSBE) 2019 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Research conference on applications of control engineering in Systems and Synthetic Biology; interdisciplinary audience.
Year(s) Of Engagement Activity 2019
URL http://fosbe2019.ai2.upv.es/
 
Description Oral Presentation, Synthetic Biology UK (SBUK), Bristol 2018 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Most relevant Synthetic Biology conference in UK; interdisciplinary audience.
Year(s) Of Engagement Activity 2018
URL https://www.eventsforce.net/biochemsoc/frontend/reg/thome.csp?pageID=18045&eventID=43&traceRedir=2
 
Description Oral Presentation, Synthetic Biology: Engineering, Evolution & Design (SEED), 2019, New York (US) 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Interdisciplinary conference on Synthetic Biology.
Year(s) Of Engagement Activity 2019
URL http://synbioconference.org/2019