Overcoming insecticide resistance using diverse fungal pathogens and variable agricultural landscapes

Lead Research Organisation: University of Stirling
Department Name: Biological and Environmental Sciences

Abstract

Successful agriculture requires crop defence against insects. Brazil's agricultural economy suffers annual yield losses of 14.7 billion US$ from insect pests. Indeed, insects consume 10-20% of all global crops whilst growing or in storage. Agriculture relies on widespread chemical insecticide application. Despite extensive research on insecticide application regimes, insecticidal compounds and genetically modified crops, insects continue to evolve resistance against control agents with predictable regularity. The vast scale of Brazilian agriculture and the levels of insecticide use required for pest control have driven resistance evolution in several significant insect pests. Our pump-priming project assesses how 'biopesticide' fungi that naturally kill insects can be used to sustainably control a major new pest of many Brazilian crops, the invasive cotton bollworm, Helicoverpa armigera.

The logic for our project comes from evolutionary science and the particular features of host-pathogen interactions. Insecticide resistance evolution occurs when a single control agent is applied over a broad area, then consistent evolutionary pressures drive previously-rare resistance genes to spread rapidly through the pest population. To prevent this, our project studies how multiple fungal pathogen strains can be used in a spatial matrix across agricultural landscapes, so that selection for resistance varies in different locations, preventing a uniform evolutionary response. On its own, this approach may not be sufficient because of cross resistance: genes making pests resistant to one fungal strain could also confer resistance to others. However, in host-pathogen systems, the optimum genotype to defend against one pathogen is often highly sensitive to the organism's environment. Simultaneous manipulation of an environmental landscape variable (the type of crop grown by farmers) will substantially decrease the consistency of selection: we predict this will prevent resistance evolution.

To develop a pest control system using these principles, our pump priming project integrates research in Brazil and the UK to:

1. Quantify the extent to which genes making cotton bollworm resistant under one combination of fungal pathogen and crop plant make them susceptible in another pathogen-crop combination. We will measure these genetic relationships in a large lab experiment, taking many pest genotypes, allowing each to feed on one of three crops, then exposing them to different fungal strains.

2. Establish that multiple fungal pathogen strains can kill cotton bollworm larvae and have desirable pest control properties: e.g. rapid kill rate, feeding suppression, and negative effects on adult survivors. We will do this by testing larval responses to many fungal isolates in the lab.

3. Show that fungal pest control qualities are maintained under temperature and humidity conditions commonly occurring in Brazilian fields. Lab infection experiments will manipulate environmental variables and assess larval mortality. Then we will spray spores onto crops in the field and test how viability and pest pathogenicity degrade over time.

After this pump priming phase, our future work will assess: spatial scales for deploying crop types and fungal biopesticides to block resistance evolution; details of potential evolutionary responses in pests; and economics of implementation.

The whole project will generate a suite of fungal pathogens and recommendations for their application in managed agricultural landscapes comprising multiple crop species to block resistance evolution. With these outputs and our industry/NGO partners we will commercialise a cost effective, practical and evolution-proof pest control system for Brazil.

Planned Impact

Our work has several clear beneficiaries. We summarise them below, along with explanations for how each will benefit.

Farmers and the farming economy

Our ambition to develop a sustainable system of pest management using fungal biopesticides will benefit individual farmers and the broad agricultural economy. Farmers will be able to better control insect pests, and consequently harvest higher yields, while the increased stability achieved by decreasing susceptibility to resistant herbivore outbreaks will minimize losses and insurance claims, and reduce overall volatility in the agricultural sector. If our project is successful, the benefits could extend well beyond São Paulo State and be useful for the types of agricultural systems across Brazil (and indeed elsewhere globally).


Industrial partners

A sustainable pest control technology presents advantages to the industrial partner(s) who develop formulations from our initial isolates. Although the work involved in formulating fungal biopesticides is not trivial, companies will be much more likely to recover their investments and secure good profits if the technology is applied in a way that minimizes the chance of pest resistance evolution, and therefore secures the long-term financial rewards of investing in research and development on biopesticides. Because increasing environmental complexity may help expose costs of resistance to other pest-control measures, our system may also incidentally help rescue some technologies that are no longer effective thanks to previous insecticide resistance; such an outcome would help not only farmers but also producers of other pest control products that have suffered rapid evolution of pest resistance (e.g. some Bt cultivars).


The wider public

Being able to manage insect pests more predictably is likely to improve food security in both developed and developing nations. If implemented on a wide scale in the future, we expect that a more heterogeneous worldwide agricultural landscape in combination with an arsenal of appropriate biopesticides, will stabilise food prices and help minimize local food shortages that are a source of suffering, inequality and conflict. In addition, the public will benefit from reduced ecological costs of current pest control practices. For example, chemical pesticides are known to have adverse consequences for nontarget organisms living on or near agricultural land. The benefits to ecosystems will pay dividends to the public, because of the many services that healthy ecosystems provide, including to agriculture, for example, in the form of plant pollination and predation of pests.

Publications

10 25 50
 
Description We now have compelling evidence supporting our framework for sustainable insect pest control using variable fungal biopesticides and crop heterogeneity; our results demonstrate the potential to harness inconsistent selection arising from landscape variability to prevent resistance evolution in pests.
1. We show that changing fungal isolates used in biopesticides and applying these to different crops may provide a powerful tool to prevent resistance evolution by making selection on pests inconsistent;
2. We identified a suite of fungal isolates with powerful toxicity to H. armigera and characterised how isolates vary in their sublethal effects;
3. We verified that these fungi can deliver robust kill rates on H. armigera after spraying crops in the field in Brazil, confirming their potential as biopesticides.
Exploitation Route Our Stage II grant is currently developing this research as a practical option for sustainable agriculture. Along with a large team of partners and collaborators, we seek to overcome three challenges to implementing this solution in the modern agricultural context: 1) We will assess whether heterogeneous environments produced by crop and fungal biopesticide combinations can delay the evolution of resistance in the long term; 2) We will evaluate whether our fungal isolates are suitable for commercial production, the extent to which we can deliver them viably in the field, and quantify their control pests at the field scale; and 3) We will identify social and economic barriers in the agricultural community to uptake of this pest control system, and investigate solutions to overcome these barriers.
Sectors Agriculture, Food and Drink,Environment

 
Description 21ROMITIGATIONFUND Stirling
Amount £238,000 (GBP)
Funding ID BB/W510713/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 07/2021 
End 03/2022
 
Description Assessing the market potential of a novel resistance management framework for fungal biopesticides in the UK glasshouse horticulture
Amount £19,673 (GBP)
Funding ID BB/T010142/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 07/2019 
End 02/2020
 
Description How does fluctuating selection affect pesticide effectiveness?
Amount £80,000 (GBP)
Funding ID 2436397 
Organisation Natural Environment Research Council 
Sector Public
Country United Kingdom
Start 08/2020 
End 03/2024
 
Description Landscape heterogeneity as a sustainable management strategy to reduce evolution of resistance and decrease use of pesticides
Amount 3,600,000 kr (SEK)
Funding ID 2021-05466 
Organisation Swedish Research Council 
Sector Public
Country Sweden
Start 01/2022 
End 12/2025
 
Description Stage 2 BBSRC-FAPESP Newton Fund call on AMR and insect pest resistance in agriculture and livestock
Amount £620,956 (GBP)
Funding ID BB/S018956/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2019 
End 04/2021
 
Description Stirling Spark: Sustainable Futures for Kenyan Crop Protection
Amount £14,803 (GBP)
Organisation University of Stirling 
Sector Academic/University
Country United Kingdom
Start 12/2019 
End 07/2020
 
Description Sustainable futures for Kenyan crop protection - Internal Stirling University GCRF Spark Grant
Amount £14,803 (GBP)
Organisation University of Stirling 
Sector Academic/University
Country United Kingdom
Start 12/2019 
End 05/2020
 
Description The stability of genetic constraints on pesticide resistance evolution
Amount 600,000 kr (SEK)
Funding ID CTS 20:63 
Organisation Carl Tryggers stiftelse för vetenskaplig forskning 
Sector Public
Country Sweden
Start 11/2021 
End 10/2023
 
Description Bid for BBSRC-NERC call - Molecules to Landscapes: building interdisciplinary capabilities 
Organisation Fera Science Limited
Country United Kingdom 
Sector Public 
PI Contribution Building on our track record build up though these existing grants I have assembled a consortium from across academia and industry to put a bid into the above call. Our bid is titled: REMBIO: Resistance management for the new era of biocontrol-based crop protection.
Collaborator Contribution Partners bring expertise, especially in field trials and IPM specialisms, alongside industry contacts.
Impact This application is multi-disciplinary - it involves parasite biologists, genomics, evolutionary biologists, IPM experts, landscape modellers, and industry. We have produced a grant application which will be submitted March 24th 2022.
Start Year 2022
 
Description Bid for BBSRC-NERC call - Molecules to Landscapes: building interdisciplinary capabilities 
Organisation Forest Research
Country United Kingdom 
Sector Public 
PI Contribution Building on our track record build up though these existing grants I have assembled a consortium from across academia and industry to put a bid into the above call. Our bid is titled: REMBIO: Resistance management for the new era of biocontrol-based crop protection.
Collaborator Contribution Partners bring expertise, especially in field trials and IPM specialisms, alongside industry contacts.
Impact This application is multi-disciplinary - it involves parasite biologists, genomics, evolutionary biologists, IPM experts, landscape modellers, and industry. We have produced a grant application which will be submitted March 24th 2022.
Start Year 2022
 
Description Bid for BBSRC-NERC call - Molecules to Landscapes: building interdisciplinary capabilities 
Organisation Harper Adams University
Country United Kingdom 
Sector Academic/University 
PI Contribution Building on our track record build up though these existing grants I have assembled a consortium from across academia and industry to put a bid into the above call. Our bid is titled: REMBIO: Resistance management for the new era of biocontrol-based crop protection.
Collaborator Contribution Partners bring expertise, especially in field trials and IPM specialisms, alongside industry contacts.
Impact This application is multi-disciplinary - it involves parasite biologists, genomics, evolutionary biologists, IPM experts, landscape modellers, and industry. We have produced a grant application which will be submitted March 24th 2022.
Start Year 2022
 
Description Bid for BBSRC-NERC call - Molecules to Landscapes: building interdisciplinary capabilities 
Organisation James Hutton Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution Building on our track record build up though these existing grants I have assembled a consortium from across academia and industry to put a bid into the above call. Our bid is titled: REMBIO: Resistance management for the new era of biocontrol-based crop protection.
Collaborator Contribution Partners bring expertise, especially in field trials and IPM specialisms, alongside industry contacts.
Impact This application is multi-disciplinary - it involves parasite biologists, genomics, evolutionary biologists, IPM experts, landscape modellers, and industry. We have produced a grant application which will be submitted March 24th 2022.
Start Year 2022
 
Description Bid for BBSRC-NERC call - Molecules to Landscapes: building interdisciplinary capabilities 
Organisation Liverpool John Moores University
Country United Kingdom 
Sector Academic/University 
PI Contribution Building on our track record build up though these existing grants I have assembled a consortium from across academia and industry to put a bid into the above call. Our bid is titled: REMBIO: Resistance management for the new era of biocontrol-based crop protection.
Collaborator Contribution Partners bring expertise, especially in field trials and IPM specialisms, alongside industry contacts.
Impact This application is multi-disciplinary - it involves parasite biologists, genomics, evolutionary biologists, IPM experts, landscape modellers, and industry. We have produced a grant application which will be submitted March 24th 2022.
Start Year 2022
 
Description Bid for BBSRC-NERC call - Molecules to Landscapes: building interdisciplinary capabilities 
Organisation Newcastle University
Country United Kingdom 
Sector Academic/University 
PI Contribution Building on our track record build up though these existing grants I have assembled a consortium from across academia and industry to put a bid into the above call. Our bid is titled: REMBIO: Resistance management for the new era of biocontrol-based crop protection.
Collaborator Contribution Partners bring expertise, especially in field trials and IPM specialisms, alongside industry contacts.
Impact This application is multi-disciplinary - it involves parasite biologists, genomics, evolutionary biologists, IPM experts, landscape modellers, and industry. We have produced a grant application which will be submitted March 24th 2022.
Start Year 2022
 
Description Bid for BBSRC-NERC call - Molecules to Landscapes: building interdisciplinary capabilities 
Organisation Rothamsted Research
Country United Kingdom 
Sector Academic/University 
PI Contribution Building on our track record build up though these existing grants I have assembled a consortium from across academia and industry to put a bid into the above call. Our bid is titled: REMBIO: Resistance management for the new era of biocontrol-based crop protection.
Collaborator Contribution Partners bring expertise, especially in field trials and IPM specialisms, alongside industry contacts.
Impact This application is multi-disciplinary - it involves parasite biologists, genomics, evolutionary biologists, IPM experts, landscape modellers, and industry. We have produced a grant application which will be submitted March 24th 2022.
Start Year 2022
 
Description Bid for BBSRC-NERC call - Molecules to Landscapes: building interdisciplinary capabilities 
Organisation Scotland's Rural College
Country United Kingdom 
Sector Academic/University 
PI Contribution Building on our track record build up though these existing grants I have assembled a consortium from across academia and industry to put a bid into the above call. Our bid is titled: REMBIO: Resistance management for the new era of biocontrol-based crop protection.
Collaborator Contribution Partners bring expertise, especially in field trials and IPM specialisms, alongside industry contacts.
Impact This application is multi-disciplinary - it involves parasite biologists, genomics, evolutionary biologists, IPM experts, landscape modellers, and industry. We have produced a grant application which will be submitted March 24th 2022.
Start Year 2022
 
Description Bid for BBSRC-NERC call - Molecules to Landscapes: building interdisciplinary capabilities 
Organisation University of Gothenburg
Country Sweden 
Sector Academic/University 
PI Contribution Building on our track record build up though these existing grants I have assembled a consortium from across academia and industry to put a bid into the above call. Our bid is titled: REMBIO: Resistance management for the new era of biocontrol-based crop protection.
Collaborator Contribution Partners bring expertise, especially in field trials and IPM specialisms, alongside industry contacts.
Impact This application is multi-disciplinary - it involves parasite biologists, genomics, evolutionary biologists, IPM experts, landscape modellers, and industry. We have produced a grant application which will be submitted March 24th 2022.
Start Year 2022
 
Description Bid for BBSRC-NERC call - Molecules to Landscapes: building interdisciplinary capabilities 
Organisation Wageningen University & Research
Country Netherlands 
Sector Academic/University 
PI Contribution Building on our track record build up though these existing grants I have assembled a consortium from across academia and industry to put a bid into the above call. Our bid is titled: REMBIO: Resistance management for the new era of biocontrol-based crop protection.
Collaborator Contribution Partners bring expertise, especially in field trials and IPM specialisms, alongside industry contacts.
Impact This application is multi-disciplinary - it involves parasite biologists, genomics, evolutionary biologists, IPM experts, landscape modellers, and industry. We have produced a grant application which will be submitted March 24th 2022.
Start Year 2022
 
Description Developing ecologically sustainable biopesticides for cocoa smallholders in Ghana - Partnership with Ghana Cocoa Board for GCRF AgriFood Africa Innovation Awards 
Organisation Cocoa Research Institute of Ghana
Country Ghana 
Sector Academic/University 
PI Contribution We have partnered with the Cocoa Research Institute of Ghana, part of the Ghana Cocoa Board - the research wing of this major industrial partner. Ghana Cocoa Board manages the cocoa industry in Ghana. We have held online collaborative meetings and submitted a grant proposal to KTN for a GCRF AgriFood pump priming grant worth ~£40,000. We are still awaiting the outcome, pending GCRF review
Collaborator Contribution CRIG researchers have collaborated with us to share their knowledge of novel pest control interventions for cocoa, which intersect closely with our research activities.
Impact Grant Application. Note that the UKRI ODA cuts meant that this call was cancelled after the submission of proposals and no awards were made.
Start Year 2020
 
Description Exploiting pathogen diversity to develop a next generation of enhanced sustainable biopesticides - Innovate UK grant application 
Organisation Centre for Agriculture and Bioscience International
Department CABI Kenya
Country Kenya 
Sector Charity/Non Profit 
PI Contribution Using the track record I have developed as a result of these recent grants I led development of a consortium to bid for a grant from Innovate UK / GCRF through the Agritech Catalyst Round 10 call. This grant seeks to develop improved horticultural biopesticides for the Kenyan / African market. The grant is worth £999,897 in total FEC. The decision on this application is still awaited. These industry-research links in Kenya will be valuable into the future.
Collaborator Contribution Joint Innovate UK grant application.
Impact Innovate UK / GCRF grant application through the Agritech Catalyst Round 10 call Note that the UKRI ODA cuts meant that this call was cancelled after the submission of proposals and no awards were made.
Start Year 2020
 
Description Exploiting pathogen diversity to develop a next generation of enhanced sustainable biopesticides - Innovate UK grant application 
Organisation Centre for Agriculture and Bioscience International
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution Using the track record I have developed as a result of these recent grants I led development of a consortium to bid for a grant from Innovate UK / GCRF through the Agritech Catalyst Round 10 call. This grant seeks to develop improved horticultural biopesticides for the Kenyan / African market. The grant is worth £999,897 in total FEC. The decision on this application is still awaited. These industry-research links in Kenya will be valuable into the future.
Collaborator Contribution Joint Innovate UK grant application.
Impact Innovate UK / GCRF grant application through the Agritech Catalyst Round 10 call Note that the UKRI ODA cuts meant that this call was cancelled after the submission of proposals and no awards were made.
Start Year 2020
 
Description Exploiting pathogen diversity to develop a next generation of enhanced sustainable biopesticides - Innovate UK grant application 
Organisation Dudutech
Country Kenya 
Sector Private 
PI Contribution Using the track record I have developed as a result of these recent grants I led development of a consortium to bid for a grant from Innovate UK / GCRF through the Agritech Catalyst Round 10 call. This grant seeks to develop improved horticultural biopesticides for the Kenyan / African market. The grant is worth £999,897 in total FEC. The decision on this application is still awaited. These industry-research links in Kenya will be valuable into the future.
Collaborator Contribution Joint Innovate UK grant application.
Impact Innovate UK / GCRF grant application through the Agritech Catalyst Round 10 call Note that the UKRI ODA cuts meant that this call was cancelled after the submission of proposals and no awards were made.
Start Year 2020
 
Description Sustainable biopesticide use in Kenyan horticulture 
Organisation University of Nairobi
Country Kenya 
Sector Academic/University 
PI Contribution We initiated a collaboration with Dr. George Ong'amo last year that brings our expertise in biopesticides, insect pathology and evolutionary biology to Kenya in order to explore applications of the evolutionarily sustainable pest control approach we developed in our BBSRC funded research.
Collaborator Contribution Dr. George Ong'amo and his Kenyan team provide strong skills in the field ecology of insect crop pests, and facilitate interactions with key stakeholders in the Kenyan horticulture research and pest control sectors.
Impact NA; outputs still pending.
Start Year 2019
 
Description A conference presentation by Danielle Mackenzie to Association of Applied Biologists IPM Meeting: Fungal biopesticide impacts on non-target insects 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact This was the first presentation of our new data on how fungal biopesticide isolates affect non-target beneficial insects that may provide important ecosystem services in agricultural landscapes. There was useful discussion afterwards prompting new analysis options and suggesting further work to follow up.
Year(s) Of Engagement Activity 2021
 
Description A conference presentation by Matthew Tinsley: BSP Parasites Online Meeting 2021 - What insect resistance against fungal biopesticides can teach us about G x E in host-pathogen interactions 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact This was a conference talk for an online society meeting (BSP).
Year(s) Of Engagement Activity 2021
 
Description A conference presentation by Rosie Mangan to Association of Applied Biologists IPM Meeting: Fluctuating selection and enhancing diversity to overcome insecticide resistance evolution 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact This was a conference presentation giving an update on our work. The audience provided useful feedback on our study to shape publication options.
Year(s) Of Engagement Activity 2021
 
Description A research talk by Matthew Tinsley to Aberdeen Entomological Club: Managing the potential for insect pests to evolve resistance against pathogens used as agricultural biopesticides 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Policymakers/politicians
Results and Impact I delivered a research seminar on our grant funded outcomes. This led to useful discussions from the audience and has facilitated a new interdisciplinary collaboration (grant application) with one audience member.
Year(s) Of Engagement Activity 2021
URL https://www.facebook.com/AberdeenEntomologicalClub/
 
Description Bussière Zoology Seminar December 2021 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact This was a presentation for the Zoology Seminar Series coordinated by the dept. of Biological and Environmental Sciences at the University of Gothenburg.
Year(s) Of Engagement Activity 2021
 
Description Conference Talk - CABI-IOBC Latin America - Ricardo Polanczyk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact This meeting was a conference bringing a mix of academic and development sector workers together for a research update meeting.
The talk title was: Importance of microbial pest control for sustainable agriculture / Importancia do controle microbiano de pragas para a agricultura sustentavel
Year(s) Of Engagement Activity 2020
 
Description Dr. Rosie Mangan Presentation at the 2019 Congress of the European Society for Evolutionary Biology in Turku, Finland 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dr. Rosie Mangan presented our work at a special Symposium on Human-Induced Evolution at the 2019 Congress of the European Society for Evolutionary Biology in Turku, Finland. The research talk generated much discussion and an engaging series of questions and answers with evolutionary biology researchers in attendance. It also sparked the interest of the editors of scientific journals for possible publication routes.
The included link provides a video archive of the session in which Rosie presented.
Year(s) Of Engagement Activity 2019
URL https://www.rajulive.fi/streams/session6-thursday-morning/
 
Description Dr. Rosie Mangan Presentation at the 2020 Irish Ecological Association Ecology and Evolution Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact The Irish Ecological Association: 3rd Ecology and Evolution Ireland Conference 8th January 2020.
Speaker: Rosie Mangan
Authors: Rosie Mangan, Matthew Tinsley, Luc Bussiere. University of Stirling
Title: Fluctuating selection and enhancing diversity to overcome insecticide resistance evolution
The talk spurred discussion and debate, and audience members approached Rosie for more information.
Year(s) Of Engagement Activity 2020
 
Description Engagement activities and interviews in 5 Brazilian States: São Paulo, Rio Grande do Sul, Goias, Tocantins, Parana 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact The CAB Intl Brazil team lead interaction and workshops with farmers' groups to discuss insecticide resistance and get information about farmers' experience on IPM in 5 Brazilian States: São Paulo, Rio Grande do Sul, Goias, Tocantins, Parana
Year(s) Of Engagement Activity 2019
 
Description GCRF Networking Meeting at Stirling University 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact This was a workshop to promote collaborative research opportunities between Stirling University and researchers in India, specifically those involved in Plant Protection and Plant Breeding. Dr. Matthew Tinsley presented our work, which led to discussions on the potential for on-farm culturing of fungal entomopathogens on Indian farms. Discussions of possible future funding bids are ongoing.
Year(s) Of Engagement Activity 2020
 
Description Hosted and presented a Round Table Symposium at Brazilian Congress of Entomology 2018: Can general evolutionary principles improve sustainable insecticide resistance management? 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact The Brazilian Congress of Entomology is one of the most important international meetings in Latin America, and therefore offered an excellent opportunity to present our ideas and solicit feedback. Our round table included four presentations by members of our research team (detailed below), but also a series of survey questions directed to the audience to assess opinions on the effectiveness and limitations of biopesticides, the most important constraints on increasing heterogeneity in agricultural landscapes, and the most important directions for future work. The audience seemed to enjoy the interactive nature of our workshop, and over 30 members engaged with our survey to provide valuable data on the most critical directions for future research. Two of the central challenges in our Stage 2 bid for funding (submitted in October) arose in part because of discussions during this workshop on the importance of spore persistance and the issue of compatibility between entomopathogens and agriculturally important fungicides.

Round table schedule:
Insect pest resistance and its implications on food security; Presenter: Dr. Yelitza Colmenarez, 10:00 -10:15
Helicoverpa armigera resistance background; Presenter: Dr. Ricardo Polanczyk, 10:15-10:30
Interactive survey and discussion on the current state of affairs in pest resistance management, 10:30 -10:50
Fluctuating selection and the maintenance of genetic variation for susceptibility Presenter: Dr. Luc Bussie`re, 10:30 -10:50
Why insects vary in susceptibility to entomopathogens, such as Beauveria bassiana, and what this can tell us about insecticide resistance evolution
Presenter: Dr. Rosie Mangan, 11:10 -11:30
Discussion of opportunities and challenges in improving resistance management by increasing the diversity of agricultural landscapes, 11:30-12:00
Year(s) Of Engagement Activity 2018
URL https://www.cbe2018.com.br/en/programme/scientific-activities#sala-cipreste-2
 
Description Impact Workshop at Brazilian Congress of Entomology 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact At the 2018 Congress of Entomology, in addition to the Symposium we organised (described elsewhere), we also invited a select stakeholders to discuss our proposal for increasing the diversity of agricultural landscapes to enhance diversity. Attendees included scientists from several agribusiness companies (Bayer, FMC Corp), from Embrapa, and from CABI Brazil. We explained the contours of our project and sought feedback on the most important additional research required to make our work more attractive for future industrial investment. This feedback was instrumental in shaping our Stage 2 bid for funding (submitted to BBSRC and FAPESP in October 2018). We are happy to note that all participants agreed to contribute to future impact events if our project is successful in winning funding.
Year(s) Of Engagement Activity 2018
 
Description Industry online talk - Bionat Company - talk by Ricardo Polanczyk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact Delivery of an online talk entitled - Compatibility of chemical defenses and microbial products / Compatibilidade de defensive quimicos e produtos microbianos
This talk was part of industry liaison work to raise awareness of sustainable pest control practices in IPM.
Year(s) Of Engagement Activity 2020
 
Description Industry online talk - Bionat Company - talk by Ricardo Polanczyk 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact Online talk delivered to industry entitled Application of Bacillus thuringiensis in the control of caterpillars / Aplicacao de Bacillus thuringiensis no controle de lagartas.
The talk aimed to increase awareness of IPM and facilitate further industry - academic collaboration.
Year(s) Of Engagement Activity 2020
 
Description Institutional seminar UNAM by Dr. Ricardo Polanczyk: "Approaches to improve biopesticide use in IPM systems" 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact This seminar was part of a special monthly series and was disseminated electronically to many branch campuses as well as the local audience. Following the seminar, Dr. Polanczyk received many questions and engaged in prolonged discussions about the relevance of our approach for agriculture across Latin America. Ongoing discussions are underway to collaborate with UNAM scientists on future work.
Year(s) Of Engagement Activity 2020
 
Description Interview in Irish Times of Dr. Rosie Mangan 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact The interview concerned Rosie's returning to work in science after a brain injury, which she suffered in Brazil while conducting her postdoctoral research with the ENDORSE project. The piece was intended to raise awareness about brain injuries in general, while also shedding light on the challenges and prospects for recovery. Rosie's story is inspiring, and while obviously this aspect of outreach was never an intended output of our research, it is nice that her perseverance and courage might inspire others who have been affected by traumatic injuries. In addition, the interview ended with a brief comment about resistance evolution, which is likely to have reached a large audience.
Year(s) Of Engagement Activity 2020
URL https://www.irishtimes.com/news/science/i-knew-something-was-desperately-wrong-with-me-because-i-cou...
 
Description Invited seminar presentation, University of Sussex 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Luc Bussiere gave a seminar in the University of Sussex titled, "Exploiting theory on mate choice evolution to make agricultural landscapes more sustainable". The talk was attended by just under 50 participants, and provoked substantial discussion afterwards and in postgraduate discussion groups. Postgrads were also encouraged to comment on Twitter, and many did so.
Year(s) Of Engagement Activity 2020
 
Description Latin American Biological Control Symposium, Chile, October 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presentation by Dr. Yelitza Colmenarez of the Endorse project objectives and main topics at the Latin American Biological Control Symposium- Chile, October 2019. Discussed with key participants the importance of managing insect resistance to pesticides and advocated use of alternative methods of control, such as Biological Control. This is a topic of wide interest in all of Latin America. As a result of the participation in Chile, I got invited to present the project in more detail and to work as part of the Organizer and scientific Committee of the 1st SYMPOSIUM OF RESISTANCE TO INSECTICIDES AND ACARICIDES, which will happen in Brazil, in June 2020.
Year(s) Of Engagement Activity 2019
 
Description Luc Bussière Invited Presentation, GGBC Annual Meeting 2021 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact This was an invited presentation (10 mins) on our ongoing research presented to the Gothenburg Global Biodiversity Centre, a network of scientists in government, industry, non-government organisations and academia that deals with issues concerning biodiversity in a broad context
Year(s) Of Engagement Activity 2021
 
Description Meetings with Embrapa Soy Bean, October 2019 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Dr. Yelitza Colmenarez and CABI Brazil Team lead discussions with target people from Embrapa Soy Bean about the importance of the ENDORSE project, its objective and main activities to be implemented in Brazil, October 2019.
Year(s) Of Engagement Activity 2019
 
Description Participation in Postgraduate Course on the Evolution of Resistance in Agricultural Systems, SLU 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Luc Bussière was invited to participate as an instructor in this postgraduate course, organised by the Swedish University of Agricultural Sciences (SLU). Over two days, participants heard talk from active scientists studying the phenomenon of resistance evolution, and reflected on the state of research and areas for future progress.
Year(s) Of Engagement Activity 2020
 
Description Presentation at Society of Invertebrate Pathology Conference / International Organisation for Biological Control 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dr. Matthew Tinsley presented our work in a symposium on fungi as biocontrol agents. The presentation led to links with new scientific colleagues, and led to networking opportunities in Africa that will be exploited for future research plans and bids.
Year(s) Of Engagement Activity 2019
URL http://www.sipweb.org/docs/abstract-program2019Valencia.pdf
 
Description Presentation by Dr. Ricardo Polanczyk at IUPAC 2019 in Ghent, Belgium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dr. Polanczyk presented work on using encapsulated particles along with fungi as part of integrated pest management, and included some material on the BBSRC/FAPESP-funded work. The audience (primarily nanotechnology experts) reported interest in how technology might be used to further enhance environmental heterogeneity.
Year(s) Of Engagement Activity 2019
URL https://www.iupac2019.be/
 
Description Presentation during the IOBC-NTRS Webinar in July 2020 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Dr. Yelitza Colmenarez presented the project during a web-conference for the International Organisation on Biological Control. The conference was well attended, with a total of 185 participants from different countries in Latin America. There was a good level of interest in the project, and audience members reported changing their views on approaches to pesticide resistance management.
Year(s) Of Engagement Activity 2020
 
Description Presented a talk at the 2nd Ecology and Evolution Ireland Conference: Enhancing diversity to overcome resistance evolution; host-pathogen evolutionary genetics for sustainable insect pest control 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact The IEA conference is for academic and professional ecologists working in higher education and research institutions, government and non-government organisations, ecological consultancies and other professionals in ecology. This conference provided an ideal setting for cross-organisational discussion on topics including ecological intensification in agriculture, sustainable agriculture and how research can be used in policy change.
Year(s) Of Engagement Activity 2019
URL https://www.marine.ie/Home/sites/default/files/MIFiles/Docs_Comms/2nd%20Ecology%20%26%20Evolution%20...
 
Description Rosie Mangan presentation, Royal Entomological Society- Scottish Regional Meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Speaker: Rosie Mangan
Authors: Rosie Mangan, Matthew Tinsley, Luc Bussiere. University of Stirling
Fluctuating selection and enhancing diversity to overcome insecticide resistance evolution
The talk spurred interest in new approaches to manage insecticide resistance evolution.
Year(s) Of Engagement Activity 2020
 
Description Seminar presentation at Rhodes University, South Africa, by Dr. Rosie Mangan in May 2019: Harnessing ecological and evolutionary science to manage resistance evolution in agricultural pests 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Invited by Martin Hill, the director of The Centre for Biological Control and Head of Entomology, Dr. Rosie Mangan presented preliminary findings from our work on evolutionarily sustainable biocontrol, including results of quantitative genetic experiments from Stirling and practical experiments at UNESP. The talk was followed by numerous questions and discussions. Rosie was also invited to speak with PhD and Masters students regarding their research on biological control of South Africa's most significant agricultural pests and advised on medium to long insect immunity experiments the students were conducting. This occasion also lead to meetings with the research staff and research associates of the Centre of Biological Control to discuss future collaborative possibilities involving the application of our principles to South African agricultural systems and possible routes of funding to begin the projects
Year(s) Of Engagement Activity 2019
 
Description Siconbiol 2019 Conference 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presentation by Dr. Yelitza Colmenarez of the Endorse project objectives and main topics at Siconbiol 2019 and Publicity of the Endorse project in the Siconbiol 2019 conference App used by participants of the events. After the presentations, different people were interested to get to know the results at the field level in Brazil. I told them that we will produce a report at the end of the project and that we only have preliminary results.
Year(s) Of Engagement Activity 2019
URL https://siconbiol.com.br/
 
Description Workshop on Evolution of Quantitative Resistance Traits in Pests, UNESP Jaboticabal, Brazil 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact I led a postgraduate course (a "discipline") for MSc and PhD students over three days that covered a review of genetic theory, the nature of selection and response in complex continuous traits, and the implications for this theory on managing resistance to biopesticides. Participants prepared oral presentations on topics related to the subject, and we engaged in discussion on the best ways to improve the evolutionary sustainability of pest control.
Year(s) Of Engagement Activity 2019