Biotechnology applications of novel chemo-enzymatic approaches to synthesise nucleoside triphosphates (NTPs)
Lead Research Organisation:
Durham University
Department Name: Chemistry
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
People |
ORCID iD |
| David Hodgson (Principal Investigator) |
| Description | Market research was performed and clear markets for NTPs were identified. A patent application was submitted in July 2018 and is pending. Further funding was sought from BBSRC under the Follow-on Fund scheme, and this was awarded in September 2019. An advert is currently out to hire a PDRA to continue this work. |
| Exploitation Route | The original work upon which this Pathfinder funding was sought is currently patent pending. The Pathfinder award allowed an IP company to seek out potential users in industry, and facilitated discussion with these parties. Significant interest was garnered and future work seeks to 'industrialise' the technology for potential licensing and application in biocatalytic processes. |
| Sectors | Chemicals,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
| Description | A patent filing was made in July 2018, published 9 January 2020 WO 2020/008209A1. Market research (Pathfinder) has proven that there is significant potential value in our technology. This evidence was used to support an additional funding bid, which was successful. A PDRA worker was in post for 17 months until November 2021. The new findings are nearing the point for submission for publication |
| First Year Of Impact | 2021 |
| Sector | Chemicals,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
| Impact Types | Economic |
| Description | Development of a Nucleoside Diphosphate Platform for the Chemo-Enzymatic Synthesis of Nucleoside Triphosphates |
| Amount | £201,759 (GBP) |
| Funding ID | BB/T004134/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2020 |
| End | 08/2021 |
| Title | Chemo-enzymatic NTP synthesis using phosphoryl Imidazole |
| Description | We have demonstrated that a His->Gly mutant NDPK can utilise phosphoryl imidazole and NDP substrate to make an NTP. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | These preliminary results are being expanded by two PhD students. |
| Description | Academic visitor from Turku, Finland (Satu Mikkola) |
| Organisation | University of Turku |
| Country | Finland |
| Sector | Academic/University |
| PI Contribution | The visitor received training in protein production |
| Collaborator Contribution | The visitor has provided capillary electrophoresis expertise that will be used in an upcoming publication of the work |
| Impact | No outputs |
| Start Year | 2018 |
| Title | TRIPHOSPHORYLATION REACTION |
| Description | The disclosure provides a method of producing a triphosphate compound. The method comprises (a) dissolving imidazole in an aqueous alkaline solution to make an imidazole solution; (b) dissolving phosphoryl halide in an organic solvent to make an organic solution; (c) contacting the imidazole solution and the organic solution to produce a solution comprising phosphorylated imidazole (PIm); (d) removing the organic solvent from the solution comprising PIm; and (e) contacting the PIm with a mutant of nucleoside diphosphate kinase (NDPK) and a diphosphate compound, and thereby producing a triphosphate compound. |
| IP Reference | WO2020008209 |
| Protection | Patent application published |
| Year Protection Granted | 2020 |
| Licensed | No |
| Impact | Interaction with potential industrial users to identify key additional works that must be performed to explore financial viability of the approach. These findings were gained through Pathfinder funding, which also supported a successful Follow-on Funding bid. This additional funding will seek to make the system more robust and translate to a solution for industrial biocatalysts. |
| Description | Invited Departmental Seminar, Department of Chemistry, Helsinki |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | I delievered a research lecture containing material related to several projects. This may lead to the development of new collaborations. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited lecture, Department of Chemistry, Texas Christian University, USA |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | I delivered a lecture. Future collaboration may result. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Level 4 undergraduate lecture |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Undergraduate students |
| Results and Impact | I incorporated the NDPK story into an undergraduate lecture focused on bio organic chemistry of phosphorus. |
| Year(s) Of Engagement Activity | 2019 |