Multi-national workshop on cereal recombination

Lead Research Organisation: University of Bristol
Department Name: Biological Sciences

Abstract

 
Description What are we certain about, what do we need to know, and, in a reasonable time how can we manipulate the recombination landscape of the cereals? - Keith Edwards
- Temporal and spatial regulation. Checks and balances differ across species
- Mechanistic understanding in yeast/ Arabidopsis is promising but species with complex genomes have different outcomes
- Has increase in genome size had an impact on meiosis?
- Dynamics are different
- SPO11 activity is seen in repeats, which is where a lot of DSB hotspots are found in maize. But this DSB even distribution is not seen in Arabidopsis. What is this selecting procedure for DSBs, then what is the selecting feature for COs?
- Could we alter timing to prevent DSBs near the telomeres becoming COs to shift position?
- Need to understand the basic functions and information which is organism specific before you can manipulate recombination
- More effort needed in the area of gene conversions?
- Overexpression could be the way forward, but perhaps this may have a feedback loop which causes problems?
- Local and global aspect to why the COs are distal in wheat
- Can show Spo11 bound but not necessarily cleaving in yeast, which is likely to be the same in wheat?
- How do we get together again and take this forward for the long term?
- Can the resources available be taken forward to a wider audience?
- From breeders perspective, project and progress has been good and would like to continue to support this work in the future
- Need to get various things established in certain labs and then people speak and network to make the most of the knowledge available and try to prevent repetitive work going on in different labs
Exploitation Route Our proposed workshop brought international groups together to:
1. Exchanges of material, for instance mutant lines and antibodies
2. Exchange of expertise in the practical application of cereal cytogenetics and
genomics (genotyping, quantitative genetics) in studying and manipulating
recombination.
3. Development of joint research programmes with international funding.
Sectors Agriculture, Food and Drink

 
Description The discussions at the workshop have led to the commercial breeders, who were present at the workshop, reconsidering their breeding strategy. In addition the view that recombination is important in breeding has been further boosted due to the developments that our BBSRC slola has generated (see the output for this award), briefly this has shown which genes should be targeted in cereals to change both recombination frequency and the locations where recombination occurs.
First Year Of Impact 2021
Sector Agriculture, Food and Drink,Environment
Impact Types Economic,Policy & public services

 
Description Problems caused by introgessions
Geographic Reach Multiple continents/international 
Policy Influence Type Influenced training of practitioners or researchers
Impact Wheat breeding companies were made aware about the effect that introgressions have on recombination and why this may lead to difficulties in the future, this has caused some companies to re-examine their breeding strategies
 
Description A pipeline for efficient recombination in wheat
Amount £200,364 (GBP)
Funding ID BB/W003317/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2021 
End 03/2023
 
Description BBSRC Flexible Talent Mobility Award (FTMA)
Amount £25,000 (GGP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 07/2019 
End 12/2019
 
Title Introgression plotter added to Cerealsdb 
Description Software to allow users to screen wheat germplasm for potential introgressions from wheat relative 
Type Of Material Data analysis technique 
Year Produced 2019 
Provided To Others? Yes  
Impact At the request of the wheat breeding companies we have made this tool available via Cerealsdb so that breeders can check on the presence of possible introgressed regions in the bread wheat genome 
 
Title QTL database 
Description Upload of QTL database to Cerealsdb 
Type Of Material Data analysis technique 
Year Produced 2019 
Provided To Others? Yes  
Impact Breeders and academics are now able to download/examine various QTL-based datasets 
 
Description Collaboartion with Syngenta to exchnage knowledge on trasformation and double haploid production 
Organisation Syngenta International AG
Department Syngenta Ltd (Bracknell)
Country United Kingdom 
Sector Private 
PI Contribution exchange of personal to improve our technology in double haploid production
Collaborator Contribution Spent time at Syngenta providing input into transformation technolgy
Impact Better transformation and double haploid technology available to Bristol
Start Year 2019
 
Description Crop Genetics workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact A workshop on nitrogen use efficiency and wheat genetics was held in New Delhi from 25th Feb-1st March 2019 for 20 Indian undergraduate and postgraduate students.
Year(s) Of Engagement Activity 2019
 
Description Keynote presentation at 2019 Monogram 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Keynote, plenary lecture at Monogram 2019
Year(s) Of Engagement Activity 2019
 
Description Participation in a Meet The Scientist event at Thinktank, Science Museum, Birmingham. 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Part of MEICOM ITN network public engagement activity co-ordinated by the Sanchez-Moran/Franklin lab demonstrating fun science activities to families visiting Thinktank Science Museum during school half-term week. The emphasis was on the importance of plant meiosis research and issues of food security and climate change. Children and parents were able to participate in activities such as extraction of DNA from strawberries, loading a DNA gel, viewing plant chromosomes down a microscope and games involving learning about DNA sequencing and crop wild relatives & plant breeding. This generated plenty of questions and discussion and even quite young children were interested and able to engage in the activities.
Year(s) Of Engagement Activity 2019
URL https://twitter.com/biobosie/status/1098529947864444929