India Partnering Award: ICRISAT-Rothamsted Partnership for Enabling Gene Editing in Pulses
Lead Research Organisation:
Rothamsted Research
Department Name: Plant Sciences and the Bioeconomy
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Publications
Hahn F
(2022)
Accelerated Breeding of Cereal Crops
Hahn F
(2019)
CRISPR/Cas precision: do we need to worry about off-targeting in plants?
in Plant cell reports
Hahn F
(2020)
A modular cloning toolkit for genome editing in plants.
in BMC plant biology
Kumar S
(2021)
Editorial: CRISPR-Cas in Agriculture: Opportunities and Challenges.
in Frontiers in plant science
Mullan S
(2020)
Welfare Challenges of Dairy Cows in India Identified Through On-Farm Observations.
in Animals : an open access journal from MDPI
Nekrasov V
(2019)
Sequence-specific nucleases as tools for enhancing disease resistance in crops.
in Transgenic research
| Description | We have developed a genome editing protocol for chickpea. |
| Exploitation Route | The developed procedure could facilitate improvement of agriculturally important traits in chickpea. |
| Sectors | Agriculture, Food and Drink |
| Description | The award created opportunities for engagement and dialogue with researchers, policymakers and politicians regarding acceptance of genome edited crops. |
| First Year Of Impact | 2020 |
| Sector | Agriculture, Food and Drink,Government, Democracy and Justice |
| Impact Types | Policy & public services |
| Title | A modular cloning toolkit for genome editing in plants |
| Description | We have created an expanded cloning toolkit that contains ninety-nine modules encoding a variety of CRISPR/Cas-based nucleases and their corresponding guide RNA backbones. Among other components, the toolkit includes a number of promoters that allow expression of CRISPR/Cas nucleases (or any other coding sequences) and their guide RNAs in monocots and dicots. As part of the toolkit, we present a set of modules that enable quick and facile assembly of tRNA-sgRNA polycistronic units without a PCR step involved. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | Expansion of the range of genome editing reagents available as standardised Golden Gate cloning modules. |
| URL | https://www.biorxiv.org/content/10.1101/738021v3 |
| Description | Partnership for enabling gene editing in pulses |
| Organisation | International Crops Research Institute for the Semi-Arid Tropics |
| Country | India |
| Sector | Charity/Non Profit |
| PI Contribution | We have developed Golden Gate cloning modules for assembling CRISPR/Cas constructs for gene editing in pulse crops. |
| Collaborator Contribution | ICRISAT is providing expertise in chickpea transformation. |
| Impact | Internships by Laura Sanjurjo Loures (RREs) at ICRISAT (learning the chickpea transformation procedure) and Pradeep Bommineni (ICRISAT) at RRes (learning genome editing techniques). |
| Start Year | 2019 |
| Description | 8th Plant Genomics & Gene Editing Congress: Europe 2020 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | I gave a talk entitled "Genome editing as a tool for gene function studies and enhancement of disease resistance in crops" at the 8th Plant Genomics & Gene Editing Congress: Europe 2020 conference in Rotterdam, Netherlands. |
| Year(s) Of Engagement Activity | 2020 |
| Description | A talk at the "Plant Genomes in a Changing Environment" conference (Cambridge, UK) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Gave a talk entitled "A molecular toolkit for gene function studies using CRISPR/Cas in monocots and dicots". |
| Year(s) Of Engagement Activity | 2019 |
| Description | APPG Science & Technology in Agriculture meeting |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Participated in the APPG Science & Technology in Agriculture meeting (online). |
| Year(s) Of Engagement Activity | 2020 |
| URL | http://www.appg-agscience.org.uk/meetings.html |
| Description | Genetic Technologies Workshop organised by the Department for Business, Energy & Industrial Strategy |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | Participated in the Genetic Technologies Workshop (online) organised by the Department for Business, Energy & Industrial Strategy. |
| Year(s) Of Engagement Activity | 2020 |
| Description | ICRISAT workshop on genome editing in crops |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | ICRISAT workshop on genome editing in crops |
| Year(s) Of Engagement Activity | 2022 |
| Description | Keystone Symposium "Plant Genome Engineering: From Lab to Field" (virtual) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Presented a poster. |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://virtual.keystonesymposia.org/ks/live/596/page/4756 |
| Description | One CGIAR online conference: "Genome Editing in Agriculture: Innovations for Sustainable Production and Food Systems" |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Participated in the One CGIAR online conference: "Genome Editing in Agriculture: Innovations for Sustainable Production and Food Systems" |
| Year(s) Of Engagement Activity | 2020 |
| Description | Plant Biology 2021 Worldwide Summit (virtual) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Participated in the conference (submitted an abstract). |
| Year(s) Of Engagement Activity | 2021 |
| Description | Plant Genomics and Gene Editing Congress: Europe |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Plant Genomics and Gene Editing Congress: Europe |
| Year(s) Of Engagement Activity | 2022 |
| URL | https://www.global-engage.com/event/plant-genomics-europe/ |
| Description | Plant Science for Climate Emergency (virtual edition) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Participated in the conference (submitted an abstract). |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://www.vibconferences.be/events/plant-science-for-climate-emergency |
| Description | Plant and Animal Genomes meeting |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Plant and Animal Genomes meeting |
| Year(s) Of Engagement Activity | 2023 |
| URL | https://www.intlpag.org/30/ |
| Description | Poster presentation at the "Plant and Animal Genomes 2020" conference (San Diego, USA) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Presented a poster entitled "A molecular toolkit for gene function studies using CRISPR/Cas in monocots and dicots" |
| Year(s) Of Engagement Activity | 2020 |
| Description | Poster presentation at the MPMI (2019) conference (Glasgow, UK) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Presented a poster entitled "A molecular toolkit for gene function studies using CRISPR/Cas in monocots and dicots". |
| Year(s) Of Engagement Activity | 2019 |
| Description | Royal Society UK-Tunisia meeting for early career researchers |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Royal Society UK-Tunisia meeting for early career researchers |
| Year(s) Of Engagement Activity | 2022 |
| Description | Visit to ICRISAT |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Other audiences |
| Results and Impact | Gave a talk on genome editing in plants at ICRISAT (India) |
| Year(s) Of Engagement Activity | 2019 |
| Description | Workshop on legumes and cereals at ICRISAT |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | A one day workshop focused on legumes and cereals involved presentations by 10 speakers from ICRISAT and RRes and discussions on topics related to genomics, stress tolerance and genome engineering. |
| Year(s) Of Engagement Activity | 2020 |