China Partnering Award: Does chloroplast reactive oxygen underpin plant disease resistance?
Lead Research Organisation:
University of Warwick
Department Name: School of Life Sciences
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Publications
Allwood J
(2021)
Unravelling Plant Responses to Stress-The Importance of Targeted and Untargeted Metabolomics
in Metabolites
| Description | We have initiated this work with a Chinese lab who are experts in reactive oxygen generation and signalling in the chloroplast. The access to their mutants and expertise has allowed us to already begin to formulate new hypotheses. This has led to the acceptance of writing a joint review for the prestigious Annual Review of Plant Pathology. |
| Exploitation Route | Too early |
| Sectors | Agriculture Food and Drink Environment |
| Description | Anatomy and functions of LTP interactomes and their relationship to small RNA signals in systemic acquired resistance |
| Amount | £650,866 (GBP) |
| Funding ID | BB/X013049/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 08/2023 |
| End | 10/2027 |
| Title | Cell sorting of chloroplasts using shape and endogeneous reporters |
| Description | We discovered that chloroplasts of phytobacterial infected leaf tissues became more fragile and some changed shape. We developed chloroplast sorting and imaging protocols (and are currently fine tuning) on state-of-the-art ImageStream X MkII and BD Fortessa & BD LSRII flow cytometers. This will provide the opportunity to explore this recently discovered chloroplast heterogenity through proteomics. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2024 |
| Provided To Others? | No |
| Impact | To our knowledge there is no research onto chloroplast heterogenity under stress conditions and this concept was co-developed with our Collaborators at the Shanghai Plant Stress Centre and preliminary tests at Warwicks Cell Sorting Facility have proven the utility of this as a means to look at different cellular chloroplast processes using proteomics. |
| Description | Cross talk between CNLs and TNLs in effector triggered immunity |
| Organisation | Instituto de Hortofruticultura Subtropical y Mediterránea "La Mayora" |
| Country | Spain |
| Sector | Public |
| PI Contribution | We established the novel techniques to monitor activated R protein signalling and looked at CNL and TNL signalling and the contribution of helper (RNLs) in the process. We then approached our Malaga partners who focus on Zar1, an "orphan" CNL to look at combinatorial challenges. |
| Collaborator Contribution | Malaga have generated specific constructs to look at how effectors that activate plant disease resistance signalling cross talk to later chlorophyll dynamics - which is a surrogate readout for activation of, or suppression of plant disease signalling networks. Their contribution has been invaluable in enabling us to decipher activation dynamics and implicated competition for specific components in signal activation. |
| Impact | Paper in preparation Small workshop organised for April 2023. |
| Start Year | 2020 |
| Description | Cross talk between CNLs and TNLs in effector triggered immunity |
| Organisation | University of Malaga |
| Country | Spain |
| Sector | Academic/University |
| PI Contribution | We established the novel techniques to monitor activated R protein signalling and looked at CNL and TNL signalling and the contribution of helper (RNLs) in the process. We then approached our Malaga partners who focus on Zar1, an "orphan" CNL to look at combinatorial challenges. |
| Collaborator Contribution | Malaga have generated specific constructs to look at how effectors that activate plant disease resistance signalling cross talk to later chlorophyll dynamics - which is a surrogate readout for activation of, or suppression of plant disease signalling networks. Their contribution has been invaluable in enabling us to decipher activation dynamics and implicated competition for specific components in signal activation. |
| Impact | Paper in preparation Small workshop organised for April 2023. |
| Start Year | 2020 |
| Description | Keynote speaker at MPMI meeting, Glasgow 2019 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Keynote presentation on the role of the chloroplast in local and systemic signalling and presented unpublished data on the role of endoplasmic reticulum remodelling in plant pathogen interactions. |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://ismpmi.confex.com/ismpmi/2019/meetingapp.cgi/Person/4891 |
| Description | Presentation at the Shanghai Centre for Plant Stress Biology |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Postgraduate students |
| Results and Impact | Presented the concept of chloroplast immunity in both PAMP triggered immunity, effector triggered immunity and as a driver in generating a systemic immune signal to establish systemic acquired resistance. |
| Year(s) Of Engagement Activity | 2019 |