Development of a Nucleoside Diphosphate Platform for the Chemo-Enzymatic Synthesis of Nucleoside Triphosphates

Lead Research Organisation: Durham University
Department Name: Chemistry


Nucleoside triphosphates (NTPs) are Nature's raw materials for the assembly of the nucleic acids that store genetic material. We have developed an enzyme-based method that allows us to prepare NTPs. We use inexpensive materials in our processes, however, we are not sure how efficient or broadly-applicable our system is or how it compares to other known enzyme systems. We have gathered independent market research to show that our system could have broad interest to industrialists. Through this consultation, however, it was clear that further information on the breadth of scope and the limitations of our system were required before industry could consider adopting it. Furthermore, we also need to convert our system from something that works in a research laboratory into something that can be employed robustly on a chemical plant. The proposed work aims to broaden our understanding and translate our research towards industrial applications


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Description We set out to develop more robust enzymes and systems for exploiting out idea for the preparation of nucleoside triphosphates. We successfully achieved most of the goals we set out to achieve. Firstly, we generated a more robust enzyme that stores well without degrading. We also showed that is is able to work across a range of nucleotide substrates. We developed simple approaches towards making the substrate we use to provide a phosphate group too.
Exploitation Route We have developed more robust methods that now may offer more interest to potential partners for further developments.
Sectors Chemicals,Manufacturing, including Industrial Biotechology