Canada Partnering Award: A one health approach to understanding and using bacteriophages in the control of enteric pathogens

Lead Research Organisation: Quadram Institute
Department Name: Gut Microbes and Health

Abstract

Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.

Publications

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Anany H (2022) ICTV Virus Taxonomy Profile: Chaseviridae 2022. in The Journal of general virology

 
Title De novo sequencing of phages T4 and T7 
Description Raw data and assemblies of the de novo sequencing of the "model" phages T4 and T7, performed with Illumina NextSeq 2x150 and Oxford Nanopore, generated for the "Phage Annotation Workshop" held online on November 2021: https://github.com/quadram-institute-bioscience/phage-annotation-workshop/ 
Type Of Material Database/Collection of data 
Year Produced 2021 
Provided To Others? Yes  
Impact Datasets used as teaching materials and available for any scientist to use. 
URL https://zenodo.org/record/5704419
 
Description Research visit and partnership with Agriculture and AgriFood Canada 
Organisation Agriculture and Agri-Food Canada
Country Canada 
Sector Public 
PI Contribution Research visit to Agriculture and Agrifood Canada (AAFC), including review of the Bacteriophage lab activities (AAFC). Set of collaborative experiments started, sequencing and data analysis performed at QIB.
Collaborator Contribution Set of collaborative experiments started where lab work is performed as in-kind contribution by AAFC.
Impact Outcomes: 2022 research visit, 2021 phage annotation workshop (see other outputs).
Start Year 2021
 
Title Repository for the Phage Genome Annotation Workshop 
Description Technical repository with the files and instructions for the Phage Genome Annotation workshop 
Type Of Technology Software 
Year Produced 2021 
Open Source License? Yes  
Impact Used by the participants to the workshop, but also widely downloaded afterwards thanks to the sharing on social media. The repository received six stars, 
URL https://github.com/quadram-institute-bioscience/phage-annotation-workshop/
 
Description Oral presentation at the African Phage Symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact The Africa Phage Forum organised a virtual symposium "African Phage Symposium 2021" for all stakeholders (students, academics and NGOs) on the continent, with invited talks from international stakeholders and academics. I presented a seminar titled "Basics of phage genome annotation & classification - How to get started" which addresses one of the key knowledge gaps for African scientists, sequencing and genomics.
Year(s) Of Engagement Activity 2021
URL https://apf.phage.directory/
 
Description Phage Annotation Workshop 2021 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact We organised a 3 half-day workshop on the principles of phage genome annotation. The event was conducted live virtually and attended by researchers from the Quadram Institute, Agriculture and AgriFood Canada and the University of Guelph, Canada. Teaching materials were made available to the general public through GitHub and YouTube.
Year(s) Of Engagement Activity 2021
URL https://github.com/quadram-institute-bioscience/phage-annotation-workshop/wiki
 
Description Stand at Norwich Science Festival: Bacteriophages, can viruses be good? 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Stand at the Norwich Science Festival where school aged children could do a craft activity to learn about bacteriophages, which are good viruses. Excellent engagement and feedback with children and parents from across East Anglia, estimated 200 children attended the stand.
Year(s) Of Engagement Activity 2021
 
Description Youtube playlist: talks and screencasts from the Phage Annotation Workshop 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Video recordings of talks and practicals from the Phage Annotation Workshop.
Persistent media enables the re-use of the produced materials and datasets.
Year(s) Of Engagement Activity 2021
URL https://www.youtube.com/playlist?list=PLTOYPYSmztrdhjh0aVCmPjTKHKV3A5TCa