a state of the art facility for the study of protein trafficking in vivo
Lead Research Organisation:
University of Leeds
Department Name: Institute of Membrane & Systems Biology
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
Determining protein localisation and dynamics is important for answering many questions in biology. To understand how proteins function and are regulated in vivo, we need approaches by which we can determine where proteins go and when, as well as when and where two proteins interact. New and emerging technologies will go a long way towards helping us answer these questions. The first is the development of photo-activatable GFP (PA-GFP). By tagging proteins with PA-GFP, and then using photo-activation to observe a subset of fluorescently labelled molecules on a low fluorescent background, their fate can be accurately determined. The second is the recent developments in the GFP and RFP fluorophores that have improved behaviour in FRET, enabling us to use this approach to investigate protein-protein interactions in vivo. Furthermore, microscopy is now being developed as a tool for high throughput screening approaches, to investigate the effects of mutations, or for screening large numbers of small molecules for ones that have useful effects in cell biology. The major goal of this application is to upgrade our existing bio-imaging facility into a state-of-the-art facility that can exploit these new technologies, with the focus of studying protein trafficking in vivo.
Publications
McHale R
(2010)
Prussian blue coordination polymer nanobox synthesis using miniemulsion periphery polymerization (MEPP).
in Chemical communications (Cambridge, England)
James NJ
(2010)
The role of Cajal bodies in the expression of late phase adenovirus proteins.
in Virology
Smith AJ
(2010)
Direct endosomal acidification by the outwardly rectifying CLC-5 Cl(-)/H(+) exchanger.
in The Journal of physiology
Boyne J
(2010)
Kaposi's sarcoma-associated herpesvirus ORF57 protein interacts with PYM to enhance translation of viral intronless mRNAs
in The EMBO Journal
Mankouri J
(2010)
Optineurin negatively regulates the induction of IFNbeta in response to RNA virus infection.
in PLoS pathogens
Li J
(2011)
Orai1 and CRAC channel dependence of VEGF-activated Ca2+ entry and endothelial tube formation.
in Circulation research
Rose K
(2011)
Transcriptional repression of the M channel subunit Kv7.2 in chronic nerve injury.
in Pain
Hong Y
(2011)
Herpesvirus saimiri-based endothelin-converting enzyme-1 shRNA expression decreases prostate cancer cell invasion and migration.
in International journal of cancer
Prandovszky E
(2011)
The neurotropic parasite Toxoplasma gondii increases dopamine metabolism.
in PloS one
Scott DJ
(2011)
Clot architecture is altered in abdominal aortic aneurysms and correlates with aneurysm size.
in Arteriosclerosis, thrombosis, and vascular biology
Smith KA
(2011)
Interactions between factor XIII and the alphaC region of fibrinogen.
in Blood
Bradley H
(2011)
Residues 155 and 348 Contribute to the Determination of P2X7 Receptor Function via Distinct Mechanisms Revealed by Single-nucleotide Polymorphisms
in Journal of Biological Chemistry
Brown LA
(2011)
A small molecule with differential effects on the PTS1 and PTS2 peroxisome matrix import pathways.
in The Plant journal : for cell and molecular biology
Jackson BR
(2011)
An interaction between KSHV ORF57 and UIF provides mRNA-adaptor redundancy in herpesvirus intronless mRNA export.
in PLoS pathogens
Bottanelli F
(2011)
Vacuolar transport in tobacco leaf epidermis cells involves a single route for soluble cargo and multiple routes for membrane cargo.
in The Plant cell
Taylor A
(2011)
Mutation of a C-terminal motif affects Kaposi's sarcoma-associated herpesvirus ORF57 RNA binding, nuclear trafficking, and multimerization.
in Journal of virology
Turrell SJ
(2011)
Mutation of herpesvirus Saimiri ORF51 glycoprotein specifically targets infectivity to hepatocellular carcinoma cell lines.
in Journal of biomedicine & biotechnology
Macnab SA
(2011)
Herpesvirus saimiri-mediated delivery of the adenomatous polyposis coli tumour suppressor gene reduces proliferation of colorectal cancer cells.
in International journal of oncology
Turrell S
(2012)
Cellular uptake of highly-functionalized ruthenium(II) tris-bipyridine protein-surface mimetics
in Bioorganic & Medicinal Chemistry Letters
Hong Y
(2012)
The impact of amyloid precursor protein signalling and histone deacetylase inhibition on neprilysin expression in human prostate cells.
in International journal of cancer
Wu W
(2012)
Different NF-?B activation characteristics of human respiratory syncytial virus subgroups A and B.
in Microbial pathogenesis
Bottanelli F
(2012)
Evidence for sequential action of Rab5 and Rab7 GTPases in prevacuolar organelle partitioning.
in Traffic (Copenhagen, Denmark)
Hess K
(2012)
A novel mechanism for hypofibrinolysis in diabetes: the role of complement C3.
in Diabetologia
Description | The aim of this project was to improve our ability to use light microscopy to image cells, and within cells. The funding allowed us to buy additional equipment to upgrade our existing confocal microscopes, so that we could improve our imaging. These microscopes are used by over 20 different research groups within the Faculty of Biological Sciences at the University, and have supported a wide range of research, from imaging organelles and how they move in living plants, to imaging receptors in mammalian cells. |
Exploitation Route | The research can be used by those interested in developing treatments for infections and disease (e.g. pharma companies, clinicians). Imaging is central to understanding the healthy human organism, plants and animals. Without knowledge of how things work, it is very difficult to understand what goes wrong in disease states. The new microscopes are essential for using imaging to understand cellular processes, and in detecting what goes wrong in diseases from virus infections, to inherited mutant |
Sectors | Healthcare Pharmaceuticals and Medical Biotechnology |
URL | http://www.fbs.leeds.ac.uk/facilities/bioimaging/ |
Description | This funding provided an upgrade to our bio-imaging facility which is used by over 40 different research groups across biological sciences and medicine. It has had impact in a broad range of healthcare and biological sciences. |
First Year Of Impact | 2007 |
Sector | Healthcare,Other |
Impact Types | Economic |