Structural variations of prion proteins
Lead Research Organisation:
Roslin Institute
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
This project aims to use various techniques to probe differences in prion protein properties that may impinge on the ability of these proteins to convert to pathogenic isoforms. It also aims to characterise those sites not typically associated with susceptibility, since they are not naturally polymorphic. The project objectives are to define structural variability in prion protein mutants, to characterise the effect of mutations of PrP conversion and to map the important sites for PrP conversion based on mutation to non-naturally-occurring amino acids.
Planned Impact
unavailable
Organisations
Publications
Bibby DF
(2008)
Application of a novel in vitro selection technique to isolate and characterise high affinity DNA aptamers binding mammalian prion proteins.
in Journal of virological methods
Dear DV
(2007)
Effects of post-translational modifications on prion protein aggregation and the propagation of scrapie-like characteristics in vitro.
in Biochimica et biophysica acta
Gielbert A
(2009)
High-resolution differentiation of transmissible spongiform encephalopathy strains by quantitative N-terminal amino acid profiling (N-TAAP) of PK-digested abnormal prion protein.
in Journal of mass spectrometry : JMS
Graham JF
(2010)
Low density subcellular fractions enhance disease-specific prion protein misfolding.
in The Journal of biological chemistry
Kirby L
(2010)
Inverse correlation of thermal lability and conversion efficiency for five prion protein polymorphic variants.
in Biochemistry
Periz J
(2007)
The microneme proteins EtMIC4 and EtMIC5 of Eimeria tenella form a novel, ultra-high molecular mass protein complex that binds target host cells.
in The Journal of biological chemistry
Sanghera N
(2011)
Deciphering the molecular details for the binding of the prion protein to main ganglioside GM1 of neuronal membranes.
in Chemistry & biology
Young DS
(2009)
Effect of enzymatic deimination on the conformation of recombinant prion protein.
in Biochimica et biophysica acta
| Description | Nil return - this is an expired grant |
| Exploitation Route | Nil return - this is an expired grant |
| Sectors | Agriculture Food and Drink |
| Description | Nil return - this is an expired grant |
| Description | Talk to Haddington U3A |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | Gave a talk entitled "transmissible Alzheimers?" to a local branch of the U3A |
| Year(s) Of Engagement Activity | 2015 |