Developmental dynamics of the epigenome

Lead Research Organisation: Babraham Institute
Department Name: UNLISTED

Abstract

This Theme examines in detail the mechanisms and genome-wide dynamics of reprogramming and programming events in GCs and early embryos, in tissues during ageing as well as the biology involved. It builds directly upon the resource established in Theme 1, and adds value to it. It also involves analysis of additional epigenomes, e.g. in conditional knockouts or transgenics of epigeetic modifiers. The objectives are to determine the mechanistic basis of: (1) epigenetic reprogramming in PGCs, (2) programng in later stage GCs, (3) reprogramming in early embryos (4), programming during early lineage commitment to ICM, TE, and PE, and derived stem cells (5), imprinting and transgenerational inheritance (6,) Influence of ageing upon the epigenome of the non-replicating genome of cardiac myocytes and effects thereof upon cardiac adaptation.
 
Title ARTE Epigenetics television programme 
Description This is a film on research activities of several european epigenetics labs 
Type Of Art Film/Video/Animation 
Year Produced 2015 
Impact Popular on YouTube 
 
Description Reprogramming of epigenetic information is vital to allow changes in cell potency during major development transitions, but also to mitigate against the possibility of transmission of undesirable acquired epigenetic changes between generations. However, the mechanisms responsible for epigenetic reprogramming were largely unknown. We have elucidated mechanisms by which cytosine methylation is actively removed from the parental genomes very soon after fertilisation. We have also identified that dampening DNA methylation maintenance activity is the major cause of reprogramming in fetal germ cells and, using culture models, we show that similar principles apply in human cells as well as in mouse. We have discovered that global hypomethylation is an invariant hallmark of naive pluripotency in mice and humans.
Our detailed studies of epigenetic change during growth and development has allowed us to develop exciting novel model systems and make new discoveries about the ageing process. For example, we have characterised the mouse epigenetic clock, the importance of placental function in age-linked pregnancy complications, the epigenetic mechanisms differentiating normal and pathological cardiac hypertrophy, the nuclear architecture of senescent cells and the effects of dietary change on health-span in mice and yeast. These studies have formed the basis of the work being proposed in the current epigenetics ISP.
Exploitation Route These findings have been taken up by the community as judged by research publications that build on our results.
Sectors Education,Healthcare,Pharmaceuticals and Medical Biotechnology

URL https://www.babraham.ac.uk/our-research/epigenetics
 
Description As part of the wider Babraham Institute strategy, across the course of this ISP we have developed a much broader public engagement strategy, undertaking large prestigious events including two Royal Society exhibits specifically showcasing our work (Epigenetics: DNA is not your destiny and Race Against the Ageing Clock), as well as presentations at local science fairs and engagement with local schools. We have also contributed substantially to transferable skills development, analytical thinking and training, mentoring and career development. We have also significantly contributed to KEC activities eg helping to spin out the company Cambridge Epigenetix and continuing membership on their SAB.
First Year Of Impact 2013
Sector Healthcare,Pharmaceuticals and Medical Biotechnology
Impact Types Societal,Economic

 
Description Babraham Institute Public Dialogue 2015
Geographic Reach National 
Policy Influence Type Citation in other policy documents
Impact Improved public understanding of biological sciences and the work of the Babraham Institute
URL http://www.babraham.ac.uk/get-involved/partnerships-page/public-dialogue
 
Description CTR Board of Managers (MH)
Geographic Reach Europe 
Policy Influence Type Influenced training of practitioners or researchers
Impact Member, Board of Managers of the Centre for Trophoblast Research, Cambridge assessing funding for targetted PhD studentship and Next Generation fellowship applciations and influencing science strategy within this research area.
 
Description Chair SAB Development and Genetics Unit Institut Curie
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Participation in a advisory committee
Impact Probably the best unit of this type in France
 
Description Chair of SAB Centre for Trophoblast Research University of Cambridge
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
Impact Has changed the perception of placenta and trophoblast in reproduction
 
Description European Research Council committee member
Geographic Reach Europe 
Policy Influence Type Participation in a advisory committee
 
Description Gene editing policy
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description H2020 EU-LIFE project "LIBRA", co-lead work package "Gender Equality in Research"
Geographic Reach Europe 
Policy Influence Type Influenced training of practitioners or researchers
Impact Consideration of gender effects in research, appreciation that there may be sex differences in cell lines, animal models and treatment regimes that ought to be taken into consideration when designing studies.
 
Description Research Advisory Board, Wellbeing of Women
Geographic Reach National 
Policy Influence Type Participation in advisory committee
Impact I served as member of the scientific advisory committee to the charity Wellbeing of Women, which reviews and makes decisions on grant funding in the general field of reproductive medicine. Some of the work funded through WoW has led to improvements in health care, novel diagnostic methods, etc.
 
Description Review panel member, DFG Clinical Research Unit (Germany) (MH)
Geographic Reach Europe 
Policy Influence Type Membership of a guideline committee
Impact Funding assessment for a major research unit on male infertility which includes improvement of patient treatment options and better patient assessment and stratification. Funding was recommended by teh panel and was implemented.
 
Description Steering board Human Cell Atlas
Geographic Reach Multiple continents/international 
Policy Influence Type Participation in a advisory committee
 
Description EU BLUEPRINT
Amount £500,000 (GBP)
Funding ID 282510 
Organisation European Commission 
Department Seventh Framework Programme (FP7)
Sector Public
Country European Union (EU)
Start  
 
Description EU NoE EpiGeneSys
Amount £400,000 (GBP)
Funding ID 257082 
Organisation European Commission 
Sector Public
Country European Union (EU)
Start  
 
Description MRC Collaboration Grant (deep sequencing in Epigenomics)
Amount £960,000 (GBP)
Funding ID G0801156 
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start  
 
Description MRC iCASE training award
Amount £111,236 (GBP)
Funding ID MR/M017427/1 
Organisation Medical Research Council (MRC) 
Sector Academic/University
Country United Kingdom
Start 10/2016 
End 09/2020
 
Description Next Generation Fellowship for postdoctoral researcher Dr Paulina Latos
Amount £167,000 (GBP)
Organisation University of Cambridge 
Department Centre for Trophoblast Research
Sector Academic/University
Country United Kingdom
Start 09/2013 
End 08/2016
 
Description Wellcome Trust Strategic Award
Amount £254,000 (GBP)
Funding ID WT100160MA 
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 04/2013 
End 03/2018
 
Description Wellcome Trust Strategic Award
Amount £2,400,000 (GBP)
Organisation Wellcome Trust 
Sector Charity/Non Profit
Country United Kingdom
Start 01/2015 
End 12/2019
 
Title Single cell triple-omics 
Description Single cell triple-omics combining transcriptome, methylome, and chromatin accessibility from the same single cell. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? Yes  
Impact This method was just published. 
 
Title iTSCs 
Description Novel method that allows for the generation of trophoblast stem cells (TSCs) from fibroblasts to study development and differentiation of the placenta lineage. 
Type Of Material Cell line 
Year Produced 2015 
Provided To Others? Yes  
Impact This method makes the establishment of TSCs tremendously easier, and does not require the isolation of multiple rounds of blastocysts from time-mated females (mice). This in turn reduced the number of required animals. 
 
Title ChIP-seq profiles for Tet1 in TSCs (GSE109545) 
Description Chromatin binding profiles of Tet1 protein in trophoblast stem cells (TSCs) 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
Impact Data allow to determine the functional role of Tet1 in TSCs 
 
Title Chromatin modifications during mouse oogenesis 
Description High quality native ChIP-seq datasets for histone modifications H3K4me3, H3K27ac, H3K27me3 during oocyte growth and fully grown oocytes in the mouse. ChIP-seq datasets for H3K4me3 in oocytes genetically deficient in DNA methylation (Dnmt3a/Dnmt3b nulls). ChIP-seq datasets for oocytes genetically deficient in the major H3K4 methyltransferase MLL2 (KMT2B). Published in Hanna et al. Nat. Struct. Mol. Biol. 2018 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
Impact Recently released datasets, too early to identify impact outside of research group. 
URL https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE93941GSE93941
 
Title DMDD 
Description As part of the Wellcome Trust funded Strategic Award "Deciphering the Mechanisms of Developmental Diseases", we have generated a database in which we collect phenoytping data of the mouse placenta at various gestational stages. 
Type Of Material Database/Collection of data 
Year Produced 2014 
Provided To Others? Yes  
Impact Broad survey of embryonic lethal gene mutations for defects in embryonic and placental development. Efforts will lead, for the first time, to a global overview of the number and identity of genes, and the molecular pathways they regulate, involved in the development of the placenta as an essential organ ensuring reproductive success and health of the baby. 
URL http://www.nimr.mrc.ac.uk/news/deciphering-the-mechanisms-of-developmental-disorders-progress-report...
 
Title DNA methylation in Hira oocytes 
Description Genome-wide, single-cell DNA methylation data from mouse oocytes lacking the histone chaperone HIRA, and associated controls, as reported in Nashun et al. 2015 Molecular Cell 60, 611-625. 
Type Of Material Database/Collection of data 
Year Produced 2015 
Provided To Others? Yes  
Impact Too soon to define notable impacts, as the accompanying paper, Nashun et al. 2015 Molecular Cell, was published in October 2015. 
URL http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE66629
 
Title DNA methylation in oocytes deficient in histone modifier 
Description Genome-wide DNA methylation datasets (PBAT) and single-cell RNA-seq datasets from oocytes genetically deficient in the H3K4me3 methyltransferase MLL2 (KMT2B). Published in Hanna et al. 2018 Nat. Struct. Mol. Biol. 
Type Of Material Database/Collection of data 
Year Produced 2018 
Provided To Others? Yes  
Impact Recently released, too early to identify impact outside of research group. 
URL https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE93941GSE93941
 
Title Oocyte ChIP-seq datasets 
Description ChIP-seq datasets for selected histone modifications in mouse growing oocytes. DNA methylation profiles of oocytes lacking the histone demethylases KDM1A, KDM1B. As reported in Stewart et al., 2015 Genes and Development, 29, 2449-2462 
Type Of Material Database/Collection of data 
Year Produced 2015 
Provided To Others? Yes  
Impact Too soon to define notable impacts, as the associated manuscript was published in Genes and Development in December 2015 
URL http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE74549
 
Title Profiling of epigenetic reprogramming between ESCs, MEFs and TSCs 
Description DNA methylation profiles of various ESC->TSC reprogramming models, as well as MEF->TSC reprogramming. 
Type Of Material Database/Collection of data 
Provided To Others? No  
Impact Datasets are deposited in publicly accessible databases. Findings show that an epigenetic memory is retained when reprogramming from ECSs, but less so when MEFs are used as starting material. 
 
Title Stem Cell methylomes 
Description DNA methylation (meDIP)-seq profiling of ESCs, TSCs, XEN cells and EpiSCs 
Type Of Material Database/Collection of data 
Provided To Others? No  
Impact Data were deposited in publicly accessible databases. Data will be useful to many other researchers for meta-analyses. 
 
Title Webtool for integration of high-throughput datasets and their rapid analysis 
Description Generation of a webtool allowing rapid data analysis of high-througput sequencing datasets. 
Type Of Material Computer model/algorithm 
Year Produced 2018 
Provided To Others? No  
Impact Webtool allows to upload and integrate multiple datasets of different nature (such as ChIP-seq, RNA-seq, DNA methyaltion profiles) and allows to perform rapid multivariate analyses on them to find co-occpuancy, mutual exclusion etc 
 
Description Active Motif DNA modification antibody 
Organisation Active Motif
Country United States 
Sector Private 
PI Contribution Commercialisation of DNA modification antibodies we have made
Collaborator Contribution Commercialisation of DNA modification antibodies we have made
Impact Commercially available antibodies
Start Year 2016
 
Description Analysis of Mll2-deficient oocytes 
Organisation Technical University of Dresden
Department Biotechnology Center
Country Germany 
Sector Academic/University 
PI Contribution We framed the biological question and were responsible for experimental design and data analysis. We performed DNA methylation and histone modification analysis of oocytes conditionally deleted for Mll2 provided by the partner.
Collaborator Contribution Provided mice with conditional knock-out allele of Mll2 and Mll2-deficient oocytes.
Impact Joint publication: Hanna et al., Nat. Struct. Mol. Biol. 2018. Deposited sequence datasets at NCBI GEO: GSE93941.
Start Year 2013
 
Description Analysis of Setd1b-deficient oocytes 
Organisation Technical University of Dresden
Department Biotechnology Center
Country Germany 
Sector Academic/University 
PI Contribution We performed analysis of RNA-seq datasets in oocytes conditionally deleted for Setd1b generated by the partner, with reference to our mouse oocyte transcriptome assemblies (Veselovska et al., 2015 Genome Biol; Gahurova et al., 2017 Epigenetics & Chromatin).
Collaborator Contribution Partner was responsible for framing the project and experimental design and providing data from mice with conditional knock-out allele of Setd1b in oocytes
Impact Joint publication: Brici et al., Development 2017
Start Year 2013
 
Description Bioinformatic analysis of oocyte ChIP-seq data 
Organisation Helmholtz Zentrum München
Department Institute of Computational Biology
Country Germany 
Sector Private 
PI Contribution We provided ChIP-seq datasets for various histone modifications in mouse wild-type and genetic knock-out oocytes to enable partner to test new informatics tools they had developed.
Collaborator Contribution The partner provided new informatics methods, based on multivariate Hidden-Markov Models, to enable analysis of multiple ChIP-seq datasets, with focus on multivariate comparisons.
Impact Joint publication: Hanna et al., Nat. Struct. Mol. Biol. 2018. Deposited sequence datasets at NCBI GEO: GSE93941. Hosted visiting bioinformatics PhD student from collaborating lab. Inter-disciplinarity: partner provides modelling and informatics expertise, our group provides biological datasets.
Start Year 2016
 
Description Bioinformatics analysis of ChIP-seq and RNA-seq data 
Organisation EMBL European Bioinformatics Institute (EMBL - EBI)
Country United Kingdom 
Sector Academic/University 
PI Contribution Provided extensive high-throughput sequencing datasets for analysis.
Collaborator Contribution Expert analysis of ChIP-seq data and data mining of existing datasets
Impact Multi-discipliniary, involving mathematical biology and bioinformatics. Joint manuscript submitted October 2014
Start Year 2014
 
Description Bioinformatics analysis of ChIP-seq and RNA-seq data 
Organisation University of Toronto
Country Canada 
Sector Academic/University 
PI Contribution Provided large-scala datasets for analysis.
Collaborator Contribution Analysed the data.
Impact Latos P.A., Sienerth A.R., Murray A., Senner C.E., Muto M., Ikawa M., Oxley D., Burge S., Cox B. and Hemberger M. (2015). Elf5-centered transcription factor hub controls trophoblast stem cell self-renewal and differentiation through stoichiometry-sensitive shifts in target gene networks. Genes & Development, 29: 2435-2448.
Start Year 2014
 
Description CEGX 
Organisation Cambridge Epigenetix
PI Contribution Data generated by CASE PhD student as preliminary/pilot data for target validation of epigenetic changes in disease.
Collaborator Contribution Industrial CASE PhD studentship partner Cambridge Epigenetix (CEGX) provided help and advice in novel, proprietary technique and enabled student to perform this new method of epigenetic target validation during the placement period.
Impact PhD studentship in progress. Manuscript(s) will be generated from this work, possibly even targets for follow-on work and potential commercialisation as epigenetic biomarkers.
Start Year 2016
 
Description Collaboration with Fredrik Lanner 
Organisation Karolinska Institute
Country Sweden 
Sector Academic/University 
PI Contribution Help and assistance with multi-omics single cell sequencing and computational analysis
Collaborator Contribution Collection of human embryo cells
Impact Paper is in preparation. Embryology, single cell genomics, computation.
Start Year 2017
 
Description Consultancy Cambridge Epigenetix 
Organisation Cambridge Epigenetix
PI Contribution I am a consultant for the company
Collaborator Contribution None at present
Impact Oxidative bisulfite sequencing
Start Year 2012
 
Description DNA methylation analysis in oocytes genetically deficient for Kdm1a (Lsd1) and Kdm1b (Aof1) 
Organisation University of Texas
Department M. D. Anderson Cancer Center
Country United States 
Sector Academic/University 
PI Contribution We were responsible for framing the project and experimental design and performed genome-wide DNA methylation analysis in oocytes genetically deficient in the H3K4me2 demethylases KDM1A or KDM1B, provided by the collaborating group.
Collaborator Contribution The partner provided oocytes from mice genetically deficient in Kdm1a or Kdm1b; provided RNA-seq datasets for these oocytes.
Impact Joint publications Stewart et al., 2015 Genes & Dev; Gahurova et al. 2017 Epigenetics & Chromatin. Deposited sequence datasets at NCBI GEO: GSE73803 and GSE74549.
Start Year 2011
 
Description DNA methylation analysis in oocytes genetically deficient in Hira 
Organisation Medical Research Council (MRC)
Department MRC Clinical Sciences Centre (CSC)
Country United Kingdom 
Sector Academic/University 
PI Contribution We performed single-cell, genome-wide bisulphite sequencing of oocytes deficient in H3.3 chaperone HIRA, provided by the collaborating group; using our newly established single-cell BS-seq method (Smallwood et al., 2014 Nat. Meths).
Collaborator Contribution The partner was responsible for framing the project and experimental design and provided mouse oocytes conditionally deleted in Hira.
Impact Joint publication Nashun et al. 2015 Mol. Cell. Deposited sequence datasets at NCBI GSE66629.
Start Year 2014
 
Description Deciduoma 
Organisation National Institute of Environmental Health Sciences
Department Reproductive and Developmental Biology Laboratory
Country United States 
Sector Public 
PI Contribution Characterisation of reproductive defects in older females
Collaborator Contribution Decidualisation model to assess the effect in the absence of an implanting embryo
Impact Collaborative publication (PMID: 28874785)
Start Year 2016
 
Description Donal O'Carrol 
Organisation University of Edinburgh
Country United Kingdom 
Sector Academic/University 
PI Contribution Helped with methylome sequencing and computational work.
Collaborator Contribution Conceived and conducted the study.
Impact Publication Vasiliauskaite et al 2017. Developmental biologists, epigeneticists, computational biologists.
Start Year 2016
 
Description Gastrulation team 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Conceived of the project and lead partner. Contributing single cell multi-omics and embryology.
Collaborator Contribution Helped to conceive of the project and several experimental and computational contributions.
Impact First publication Mohammed et al 2017. Experimental embryology, single cell biology, computation, modelling, epigenetics.
Start Year 2014
 
Description Human Cell Atlas 
Organisation Broad Institute
Country United States 
Sector Charity/Non Profit 
PI Contribution Contributed to formulating the white paper of the Human Cell Atlas.
Collaborator Contribution Conceived the project and brought the international consortium together.
Impact The white paper was published Regev et al 2017. It involves single cell biologists and computational and modelling scientists.
Start Year 2016
 
Description Jenny Nichols, Austin Smith 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Contributed to conceiving studies. Epigenetic profiling and analysis.
Collaborator Contribution Conceived and conducted studies.
Impact Publications Kalkan et al 2017, Guo et al 2017. Involves embryologists, stem cell biologists, computational biologists, epigeneticists.
Start Year 2016
 
Description Jurkowski 
Organisation University of Stuttgart
Country Germany 
Sector Academic/University 
PI Contribution Helped with reagents and interpretation.
Collaborator Contribution Conceived and conducted study.
Impact Publication Stepper et al 2017. Involves biochemists, epigeneticists.
Start Year 2016
 
Description Miguel Branco for TET paper 
Organisation Queen Mary University of London
Department Blizard Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution RNA analysis of L1 trasposon
Collaborator Contribution This project was run by the Branco lab, they produced the bulk of the data and published the paper.
Impact Research publication PMID 27863519
Start Year 2015
 
Description Millipore DNA modification antibodies 
Organisation Merck
Department MilliporeSigma
Country United States 
Sector Private 
PI Contribution Commercialisation of DNA modification antibodies we have made
Collaborator Contribution Commercialisation of DNA modification antibodies we have made
Impact DNA modification antibodies
Start Year 2011
 
Description Reprogramming of MEFs towards iTSCs and establishment of defined TSC culture conditions 
Organisation University of Bonn
Country Germany 
Sector Academic/University 
PI Contribution Genome-wide DNA methylation analysis of various clones and reprogrammed cells. Bioinformatic analysis of these data. Figures for manuscripts.
Collaborator Contribution Provided DNA for the above analysis. Performed other experiments to bring this project to conclusion.
Impact Kubaczka C., Senner C.E., Araúzo-Bravo M.J., Sharma N., Kuckenberg P., Becker A., Zimmer A., Brüstle O., Peitz M., Hemberger M. and Schorle H. (2014). Derivation and maintenance of murine trophoblast stem cells under defined conditions. Stem Cell Reports, 2: 232-242. Kubaczka C., Senner C.E., Cierlitza M., Araúzo-Bravo M.J., Kuckenberg P., Peitz M., Hemberger M. and Schorle H. (2015). Direct induction of trophoblast stem cells from murine fibroblasts. Cell Stem Cells, 17: 557-568.
Start Year 2012
 
Description Single-cell epigenomic profiling of spermatogonial stem cells 
Organisation Yokohama City University
Country Japan 
Sector Academic/University 
PI Contribution Our group is providing expertise in single-cell methylation and expression profiling methods.
Collaborator Contribution Their group is responsible for framing the project and experimental design and for providing isolated mouse spermatogonial stem cells.
Impact Hosted visit by post-doc from collaborating group to initiate single cell methylation/transcription profiling. Joint grant awarded: Royal Society International Exchange Award IEC\R3\170052 (March 2018 March 2020): Analysis of spermatogonial stem cell maintenance and differentiation system by the state-of-the-art single-cell technology.
Start Year 2016
 
Description Trophoblast Stem Cells 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Generation of locus-specific DNA methylation profiles to confirm lineage identity of derived cells.
Collaborator Contribution Establishment of an organoid system that promotes the maintenance of human trophoblast stem/progenitor cells.
Impact Publication in process. This collaboration is multi-disciplinary between pathologists, physiologists and us as epigeneticists, it also involved bio-engineering helping in the design of microfluidics devices.
Start Year 2017
 
Description XIMBIO DNA modification antibodies 
Organisation Cancer Research Technology (CRT)
Country United Kingdom 
Sector Private 
PI Contribution Commercialisation of DNA modification antibodies we have made
Collaborator Contribution Commercialisation of DNA modification antibodies we have made
Impact DNA modification antibodies
Start Year 2016
 
Description single cell TSCs 
Organisation University of Cambridge
Department Department of Obstetrics & Gynaecology
Country United Kingdom 
Sector Academic/University 
PI Contribution Collaboration with Prof Steve Charnock Jones to form core of Centre of Trophoblast Research single cell transcriptomics profiles of mouse (and human) trophoblast stem cells under various conditions, as well as of placentas. Provided cells and tissues and culture media components to collaborator.
Collaborator Contribution Set up Drop-seq and optimising technique for nuclear RNA sequencing, required for syncytia. Performed sc-RNA-seq.
Impact Manuscript in preparation.
Start Year 2017
 
Description uNK cells 
Organisation University of Cambridge
Country United Kingdom 
Sector Academic/University 
PI Contribution Characterisaiton of reproductive problems in older females.
Collaborator Contribution Analysis of immune repertoire of implantation sites depending on maternal age.
Impact Collaborative publication (PMID: 28874785).
Start Year 2016
 
Title Novel Method 
Description A novel method to manipulate potency of pluripotent cells 
IP Reference US20160186207 
Protection Patent application published
Year Protection Granted 2015
Licensed Commercial In Confidence
Impact In progress
 
Description Babraham Schools Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact As part of the annual Babraham Schools' Day, my group hosted 5 GCSE and 5 A level students for half-day practical sessions in the lab.
Year(s) Of Engagement Activity 2017
 
Description Cambridge Science Festival: Molecular Explorers, Cambridge (UK) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Exhibit at Cambridge Science Festival, engaging with visitors at stall explaining the science and advances made in the area, answering questions and discussing outstanding research goals
Year(s) Of Engagement Activity 2017
 
Description Cold Spring Harbor Laboratory meeting: Stem Cell Biology, New York (USA) (VPG, CS) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Participation in confenrence, poster presentation by both post-doctoral reserachers involved in this activity (VPG and CS) and engagement in discussions and broadening of scientific horizon
Year(s) Of Engagement Activity 2017
 
Description EMBL Mammalian Genetics and Genomics Conference (MH) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Invited speaker at AMBL Conference Mammalian genetic and Genomic, Heidelberg, Germany
Year(s) Of Engagement Activity 2017
 
Description Genetics Society and BSDB Spring Meeting, Warwick, UK (MH) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Invited speaker at annual spring meeting of the Genetics Society and British Society for Developmental Biology (BSDB) in Warwick, UK (2017)
Year(s) Of Engagement Activity 2017
 
Description IFPA Meeting, Manchester, UK (MH) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Invited talk at IFPA Meeting held in Manchester, UK (2017)
Year(s) Of Engagement Activity 2017
 
Description ISSHP Meeting, Berlin, Germany (MH) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Invited talk at ISSHP Meeting held in Berlin, Germany (2017)
Year(s) Of Engagement Activity 2017
 
Description Keynote speaker, CTR Anniversary Meeting, Cambridge, UK (MH) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Keynote lecture at 10th Anniversary Meeting of the Centre for Trophoblast Research, University of Cambridge (2017)
Year(s) Of Engagement Activity 2017
 
Description Member, Board of Managers at Centre for Trophoblast Research 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? Yes
Geographic Reach International
Primary Audience Supporters
Results and Impact As member of the Board of Managers at the CTR in Cambridge we make decisions on the award of PhD scholarships, postdoctoral fellowships and direct the general focal points of local research activity in the field of placental biology.

PhD students admitted to the University of Cambridge; postdoctoral researchers' career path enhanced
Year(s) Of Engagement Activity 2013,2014,2015,2016
 
Description Podcast with Dr Kat Arney on the impact of placentation and maternal age on reproductive outcome 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Podcast as descibed above
Year(s) Of Engagement Activity 2017
 
Description Postgraduate course Epigenetics in Reproductive Biology, University of Murcia, Spain (VPG) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Postgraduate students
Results and Impact Invited talk and workshop participation as part of postgraduate course on reproductive epigenetics
Year(s) Of Engagement Activity 2016,2017
 
Description Press release - Keeping eggs fresh with epigenetics 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Press release from Babraham to accompany publication of paper 'MLL2 conveys transcription-independent H3K4me3 in the oocyte', by Hanna et al. Nat. Struct. Mol. Biol. 2018. Picked up by 9 news outlets and blogs.
Year(s) Of Engagement Activity 2018
URL https://www.babraham.ac.uk/news/2018/01/keeping-egg-cells-fresh-with-epigenetics
 
Description Radio interview DeutschlandFunk 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Radio Interview for DeutschlandFunk on the impact of maternal age on pregnancy success, irrespective of the increased risk of defects in the egg
Year(s) Of Engagement Activity 2017
URL http://www.deutschlandfunk.de/alter-des-uterus-entscheidend-junges-glueck-in-jungen.676.de.html?dram...
 
Description Royal Society Partnering Award - CS + LW, NM, SC 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Successful application for Royal Society Partnering Award. Poster 6 selected 6th form students over the course of 1 week and conducted in-depth research project. Data analysis followed at the pupils' school directly.
Year(s) Of Engagement Activity 2016,2017
 
Description STEM teacher visit 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact As part of a week-long visit to the Institute, my group hosted 3 biology A-level teachers from local schools for involvement in practical work in the lab over a 2-day period.
Year(s) Of Engagement Activity 2016
 
Description School's Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? Yes
Geographic Reach Local
Primary Audience Schools
Results and Impact provided basic explanation of early development and the importance of the placenta for growth of a baby; hands-on experience for students in staining and microscopic analysis of a mouse placenta

rose awareness of research activities and importance of basic research to understand common pregnancy-associated diseases
Year(s) Of Engagement Activity 2006,2007,2008,2009,2010
 
Description Schools Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact Project design, scientific background induction and practical supervision for 6th form students as well as teachers during Annual Schools Day.
Year(s) Of Engagement Activity 2015,2016,2017
 
Description Schools Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Groups of 5-6 6th-form students take part in a one-day visit to 2 research groups at the Babraham Institute and conduct two small research experiments during that day.
Year(s) Of Engagement Activity 2017,2018
 
Description The Ageing Cell Conference, Cambridge, UK (MH) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Invited talk at The Ageing Cell Conference held in Cambridge, UK (2017)
Year(s) Of Engagement Activity 2017
 
Description Twilight teacher training event, Bioscience lite-CRISPR-Cas9 genome editing- technology, applications and implication, Cambridge (UK) (VPG) 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Talk and Q&A session with high school teachers to update and inform them on state-of-the-art genome editing tools
Year(s) Of Engagement Activity 2017