Signalling mechanisms that determine the rate of ageing
Lead Research Organisation:
Babraham Institute
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
Ageing can be driven by the accumulation of senescent cells in a tissue causing the emergence of a SASP phenotype (Senescence-Associated Secretory Phenotype) in which senescent cells drive more senescence and loss of tissue function. Ageing can also be driven by loss of cells, including those lost by specific programmed cell death mechanisms. Age related loss of axons and synapses is a major contributor to frailty in old age. Not only does it reduce neuromuscular strength but it impairs vision, memory, cognition and many other functions. Each requires functional innervation and malfunctions when axons are lost. Our work has delineated an ancient axonal survival pathway that appears to detect damage by sensing intracellular NMN levels. If NMN levels rise, something that is normally prevented by NMNAT2-mediated conversion to NAD+, a signalling pathway involving SARM1 is engaged that culminates in axon death. We have provided evidence this pathway, in which NMNAT2 acts as a survival-factor and SARM1 as death-inducer, contributes to neurone loss seen in ageing. We have data indicating neutrophils can sense nerve damage and the emergence of SASP, suggesting their accumulation could be an early biomarker and may shape the progression of these age-related problems. Recent work has indicated there is a DNA-methylation “clock” that marks human age. The Epigenetics ISP has evidence the apparent age of this “clock” in cultured cells can be reset by brief treatment with rapamycin via an unknown mechanism.
Planned Impact
unavailable
Organisations
- Babraham Institute (Lead Research Organisation)
- Francis Crick Institute (Collaboration)
- University College London (Collaboration)
- Marche Polytechnic University (Collaboration)
- PhoreMost (Collaboration)
- The Wellcome Trust Sanger Institute (Collaboration)
- Agricultural University of Athens (AUA) (Collaboration)
- UNIVERSITY OF CAMBRIDGE (Collaboration)
- UNIVERSITY OF EDINBURGH (Collaboration)
- AstraZeneca (Collaboration)
- Max Planck Society (Collaboration)
- Cardiff University (Collaboration)
- University College Cork (Collaboration)
- Vernalis (Collaboration)
- Eli Lilly & Company Ltd (Collaboration)
- Royal Veterinary College (RVC) (Collaboration)
- Takeda Cambridge Ltd (Collaboration)
- Cold Spring Harbor Laboratory (CSHL) (Collaboration)
- UNIVERSITY OF LEEDS (Collaboration)
- UNIVERSITY OF LIVERPOOL (Collaboration)
- UNIVERSITY OF DUNDEE (Collaboration)
Publications
Abeliovich H
(2023)
Where is the field of autophagy research heading?
in Autophagy
Adalbert R
(2018)
Interaction between a MAPT variant causing frontotemporal dementia and mutant APP affects axonal transport.
in Neurobiology of aging
Adalbert R
(2018)
Interaction between a MAPT variant causing frontotemporal dementia and mutant APP affects axonal transport.
in Neurobiology of aging
Al-Mufti Y
(2023)
Molecular, Cellular, and Metabolic Fundamentals of Human Aging
Baig H
(2020)
Synthetic biology open language visual (SBOL visual) version 2.2.
in Journal of integrative bioinformatics
Balmanno K
(2023)
ERK1/2 inhibitors act as monovalent degraders inducing ubiquitylation and proteasome-dependent turnover of ERK2, but not ERK1.
in The Biochemical journal
Bargehr J
(2019)
Epicardial cells derived from human embryonic stem cells augment cardiomyocyte-driven heart regeneration.
in Nature biotechnology
Beal J
(2019)
Communicating Structure and Function in Synthetic Biology Diagrams.
in ACS synthetic biology
Bye-A-Jee H
(2018)
The RNA-binding proteins Zfp36l1 and Zfp36l2 act redundantly in myogenesis.
in Skeletal muscle
| Title | Additional file 1: of The RNA-binding proteins Zfp36l1 and Zfp36l2 act redundantly in myogenesis |
| Description | Confirmation of ablation of ZFP36L1 and ZFP36L2 in satellite cells of Zfp36L1/L2-P mice. Western blot determining the expression of ZFP36L1 and ZFP36L2 in isolated satellite cells from Zfp36L1/L2-P and control mice. (TIF 76 kb) |
| Type Of Art | Film/Video/Animation |
| Year Produced | 2018 |
| URL | https://springernature.figshare.com/articles/Additional_file_1_of_The_RNA-binding_proteins_Zfp36l1_a... |
| Title | Additional file 1: of The RNA-binding proteins Zfp36l1 and Zfp36l2 act redundantly in myogenesis |
| Description | Confirmation of ablation of ZFP36L1 and ZFP36L2 in satellite cells of Zfp36L1/L2-P mice. Western blot determining the expression of ZFP36L1 and ZFP36L2 in isolated satellite cells from Zfp36L1/L2-P and control mice. (TIF 76 kb) |
| Type Of Art | Film/Video/Animation |
| Year Produced | 2018 |
| URL | https://springernature.figshare.com/articles/Additional_file_1_of_The_RNA-binding_proteins_Zfp36l1_a... |
| Title | Additional file 2: of The RNA-binding proteins Zfp36l1 and Zfp36l2 act redundantly in myogenesis |
| Description | Characterisation of mice lacking either Zfp36l1 or Zfp36l2 (referred to as Zfp36L1-P or Zfp36L2-P mice). A. Weights of male and female mice measured from 10 to 45 days. Error bars represent SEM, two-way ANOVA with Tukey's multiple comparison test, n = 10. B. TA and gastrocnemius muscle weights from 7-week-old male and female mice. Significance was measured by unpaired two-tailed Mann Whitney test; n = 6 for Zfp36L1-P and its respective control, and n = 8 for Zfp36L2-P and its control. Red data points indicate female mice and blue data points indicate male mice for the Zfp36L2-P graph. C. Average number of satellite cells in cross-sections of TAs from 7-week-old control, Zfp36L1-P and Zfp36L2-P mice. Error bars represent SEM, significance was measured by unpaired two-tailed Student's t test; n = 5 for Zfp36L1-P and its respective control, and n = 4 for Zfp36L2-P and its control. Controls represent Cre-negative littermates. Calculations based on 10 fields of view per experiment. (TIF 201 kb) |
| Type Of Art | Film/Video/Animation |
| Year Produced | 2018 |
| URL | https://springernature.figshare.com/articles/Additional_file_2_of_The_RNA-binding_proteins_Zfp36l1_a... |
| Title | Additional file 2: of The RNA-binding proteins Zfp36l1 and Zfp36l2 act redundantly in myogenesis |
| Description | Characterisation of mice lacking either Zfp36l1 or Zfp36l2 (referred to as Zfp36L1-P or Zfp36L2-P mice). A. Weights of male and female mice measured from 10 to 45 days. Error bars represent SEM, two-way ANOVA with Tukey's multiple comparison test, n = 10. B. TA and gastrocnemius muscle weights from 7-week-old male and female mice. Significance was measured by unpaired two-tailed Mann Whitney test; n = 6 for Zfp36L1-P and its respective control, and n = 8 for Zfp36L2-P and its control. Red data points indicate female mice and blue data points indicate male mice for the Zfp36L2-P graph. C. Average number of satellite cells in cross-sections of TAs from 7-week-old control, Zfp36L1-P and Zfp36L2-P mice. Error bars represent SEM, significance was measured by unpaired two-tailed Student's t test; n = 5 for Zfp36L1-P and its respective control, and n = 4 for Zfp36L2-P and its control. Controls represent Cre-negative littermates. Calculations based on 10 fields of view per experiment. (TIF 201 kb) |
| Type Of Art | Film/Video/Animation |
| Year Produced | 2018 |
| URL | https://springernature.figshare.com/articles/Additional_file_2_of_The_RNA-binding_proteins_Zfp36l1_a... |
| Title | Additional file 3: of The RNA-binding proteins Zfp36l1 and Zfp36l2 act redundantly in myogenesis |
| Description | Transverse cross sections of the TA muscles from mice lacking either Zfp36l1 or Zfp36l2 (referred to as Zfp36L1-P or Zfp36L2-P mice), and their respective controls, recovered at 1, 5, 10 and 25 days following injection with either CTX or PBS, stained with haematoxylin (H; myofibre; pink) and eosin (E; nuclei; purple) to assess the skeletal muscle architecture. Open arrows identify centrally located nuclei, an indication of muscle regeneration. Controls represent Cre-negative littermates. Scale bars: 100 µm. Representative of n = 3. (TIF 843 kb) |
| Type Of Art | Film/Video/Animation |
| Year Produced | 2018 |
| URL | https://springernature.figshare.com/articles/Additional_file_3_of_The_RNA-binding_proteins_Zfp36l1_a... |
| Title | Additional file 3: of The RNA-binding proteins Zfp36l1 and Zfp36l2 act redundantly in myogenesis |
| Description | Transverse cross sections of the TA muscles from mice lacking either Zfp36l1 or Zfp36l2 (referred to as Zfp36L1-P or Zfp36L2-P mice), and their respective controls, recovered at 1, 5, 10 and 25 days following injection with either CTX or PBS, stained with haematoxylin (H; myofibre; pink) and eosin (E; nuclei; purple) to assess the skeletal muscle architecture. Open arrows identify centrally located nuclei, an indication of muscle regeneration. Controls represent Cre-negative littermates. Scale bars: 100 µm. Representative of n = 3. (TIF 843 kb) |
| Type Of Art | Film/Video/Animation |
| Year Produced | 2018 |
| URL | https://springernature.figshare.com/articles/Additional_file_3_of_The_RNA-binding_proteins_Zfp36l1_a... |
| Description | 1) Neuro-degenerative diseases are progressive and are often fatal and result from the loss of functional nerve cells resulting in a wide range of potential symptoms, depending on the nerves affected. Ageing results in a loss of neurones and this increases the risk of the emergence of a number of age-related illness and is a direct cause of a variety of age-related declines in nervous/cognitive function. There remain very few approaches that can prevent or reverse these processes. We have found that genetic removal of a protein called SARM1 from mice prevents them developing another genetic disease that normally leads to loss / degeneration of axons. This result has very important implications for understanding of degenerative diseases and probably the ageing process. We have also shown that SARM1 regulates the abundance of a critical metabolite nicotinamide adenine dinucleotide (NAD+) during axon degeneration. Remarkably, SARM1 has structural similarity to nucleotide-binding leucine-rich repeat (NLR) immune receptors in plants which trigger localized cell death to restrict pathogen infection. Both processes depend on closely related Toll/interleukin-1 receptor (TIR) domains which feature self-association-dependent NAD+ cleavage activity associated with cell death signaling. These studies reveal new insights into conserved pathways for cell death in animals and plants and may support the development of new therapeutis strategies for neurodegenerative disease. We have also revealed a new approach that can identify suppressors of neurodegeneration. This work has been published most recently in the journals Neurobiology of disease and Science. 2) We have identfied changes in DNA methylation patterns and lipid metabolism in mice that appear to be associated with them experiencing a "reduced food-intake" diet that extends their life-span compared to a traditional mouse diet. It seems possible that the DNA methylation changes represent a memory of dietary exposure that can control gene expression patterns (eg of key lipid metabolising genes/proteins) over long periods of time and impact life span. These important results will lead to further focus on the specifc DNA methylation marks and lipid metabolites and enzymes that are changed (published in Nature Metabolism) 3) We have identified p57KIP2, a cyclin-dependent kinase inhibitor (CDKI), as a novel target of ERK1/2 signalling and used CRISPR/Cas9 gene editing to show that it drives cell cycle arrest and senescence. This identifies new links between a cell fate signalling pathway the cell cycle machinery and may have important implications for development of novel therapeutic strategies by our pharmaceutical sector collaborators at AstraZeneca (published in Nature Communications) 4) Both ERK1/2 and ERK5 signalling pathways are critical regulators of cell fate decisions, senescence and/or are deregulated in diseases of old age including cancer and cardiovascular disease. We have studied the mechanisms of action and cellular responses to highly selective inhibitors of ERK1/2 or ERK5. For ERK1/2 inhibtors we have identified discrete effects on ERK1/2 nuclear localisation and observed that ERK1/2 may exert kinase-independent effects on expression of genes invovled in innate immune signalling. For ERK5, a hybrid protein kinase-transcription factor we have shown that ERK5 kinase inhibitors actually promote the paradoxical activation of ERK5 transcriptional transactivation domain. These results are uncovering new roles for ERK1/2 and ERK5 and providing important new information for the Biotech/Pharma sector (published in Molecular Cancer Therapeutics, Nature Communbications and European Journal of Medicinal Chemistry) 5) Recent years have seen the development of highly selective BH3 mimetic drugs that inhibit different members of the pro-survival BCL2 protein family. These drugs are touted as 'senolytics' that can selectively kill senescent cells. We have shown that different cell lineages exhibit remarkably different dependencies on specific pro-survival BCL2 proteins, assessed by the use of highly selective BH3 mimetic drugs. This lineage dependence will need to be explored further to see if it will limit the application of senolytic BH3 mimetics. These results may also have important implications for development of novel therapeutic strategies by our pharmaceutical sector collaborators at AstraZeneca (published in Nature Communications and further work ongoing) 6) We have used CRISPR/Cas9 gene editing to knockout the mRNA binding proteins ZFP36L1 and ZFP36L2, singly and together; DKO cells exhibit profound proliferative defects associated with increased expression of multiple CDKIs (work ongoing) 7) We have used CRISPR/Cas9 gene editing to knockout IKKa and IKKb, the two protein kinases responsible for activation of the NF-kappaB transcription factors for the first time in human cells. These cells exhibit defects in expression of an array of cytokines and other components of the senescence associated secretory phenotype (SASP) (work ongoing) 8) We have found that autophagy is repressed during mitosis by a switch from mTORC1 to CDK1-dependent regulation. Continuation of autophagy during mitosis (with nuclear envelope breakdown) runs the risk of DNA damage, leading to senescence or death. Since autophagy is an arbiter of senescence the fidelity of this control mechanism may be of relevance to ageing. It may also be of relevance to our pharmaceutical sector collaborators. Published in the journals Molecular Cell and Trends in Cell Biology. 9) Autophagy is an important cellular degradation pathway with a central role in metabolism as well as basic quality control, two processes inextricably linked to ageing. In a multicentre collaboration we have found that systemic autophagy inhibition induces premature ageing and age-related pathologies in mice. Remarkably, autophagy restoration provides a near complete recovery of morbidity and a significant extension of lifespan but autophagy-restored mice still succumb earlier due to an increase in spontaneous tumour formation. Thus, our data suggest that chronic autophagy inhibition confers an irreversible increase in cancer risk and uncovers a biphasic role of autophagy in cancer development, being both tumour suppressive and oncogenic, sequentially (published in Nature Communications) 10) In a collaboration with colleagues at BI we have found that the levels of IRS1 (the primary adaptor of the insulin receptor that activates class IA PI3Ks) are, almost uniquely in the whole genome, down regulated at both transcriptional and protein levels during ageing in the B-lymphocyte compartment and could indicate this is a primary determinant of the ageing phenotype of B-cells (published in Genome Biology). 11) We have collaborated with the Gems lab to reveal that changes in lipid metabolism are key factors in the emergence of ageing phenotypes in C. elegans (published in the journal Current Biology) 12) There is a growing appreciation that reactive oxygen species (ROS) are key players in normal cellular signalling by oxidising redox-sensitive cysteine residues within proteins. While high levels of ROS may be harmful and induce oxidative stress, low levels of ROS may actually be beneficial as mediators of redox signalling. In this context, enhancing ROS production in model organisms can extend lifespan, with biological effects dependent on the site, levels, and specific species of ROS. One enzyme class implicated in ROS-dependent signalling are the protein tyrosine phosphatases (PTPs), key regulators of cell-cell communication through the control of cellular phosphotyrosine levels. We have identified novel roles for PTPs in epithelial cell barrier function, identified new methdios and strategies ot idneifiy PTP substrsates and also shed new light on their redox regulation through oxidation of previously unappreicated cysteine residues. These studies are opeing up the study of PTPs, their regulation and their roles in health and disease. Recent studies in eLife, The FEBS Journal and Nature Communications. 13) Protein misfolding is a major driver of ageing-associated frailty and age-related disease pathology. Although all cells possess multiple, well-characterised protein quality control systems to mitigate the toxicity of misfolded proteins, how they are integrated to maintain protein homeostasis ('proteostasis') in health-and how their disintegration contributes to disease-is still an exciting and fast-paced area of research. We have reviewed this field identifying unifying themes, emerging concepts and unanswered questions. (The FEBS Journal) 14) We have developed new methods (Solvent Precipitation SP3 (SP4)) for enahancing recovery for proteomics sample preparation without magnetic beads 15) We have demonstrated that IKKa makes a major response to cytokine dependent NF-kB signalling, a pathway implicated in SASP. In addition we have contributed to a systems-wide analysis of immune landscapes of inflammation (published in Nature) 16) We have provided new insights into the structure, regulation and role of key protein tyrosine phosphatases (PTPs) that are involve din cell adhesion and barrier integrity 17) We have controbuted to an important study on the role Tfh cells and the germinal center in memory B cell formation &a humoral immunity after ChAdOx1 nCoV-19 vaccination 18) We have helped to define new components of the early cellualr response to Heat Shock Protein 90 Inhibition, and important protein chaperone system for normal health and a potential drug target. 19) In collaboration with the Institutes Epigenetics Programme and AsatraZeneca we have defined escape from G1 arrest during acute MEK inhibition as a criticla event in de novo gene amplifications that drive acquisition of drug resistance 20) We have demonstrated that ERK1/2 inhibitors drive the ubiquitylation and proteasome-dependent degradation of the most abundant ERK isoform - ERK2. This may be relevant to approaches to inhibit ERK1/2 signalling for cancer therapy and senescence therapy. 21) We have developed and valisated cell lines with reporter genes regulated by ERK1/2 signalling. These can be used for identification of novel regulators of ERK1/2 signalling a critical pro-ageing pathway |
| Exploitation Route | These results will be taken forward as detailed by our existing ISPG plans and through our engagement and communication activities which are already impacting thinking and strategy in the commercial sector. See Narrative Impact for examples |
| Sectors | Education Healthcare Pharmaceuticals and Medical Biotechnology |
| Description | Discussions with commercial collaborators at Takeda and Eli Lilly have led to our results changing their therapeutic strategies and planning around targeting of neuro-degenerative diseases. Our results implicating p57KIP2 in ERK-driven cell cycle arrest and defining the lineage dependence of different BH3-mimetics on cell death have also been communicated to our commercial partners in this area including AstraZeneca. Our results on the mechanism of action of different types of ERK1/2 inhibitor have been shared with Astex Pharmaceuticals as well as the wider R&D community Our results on the paradoxical activation of ERK5 by ERK5 kinas einhibtors has been shared with Oppilotech Ltd and we are exploring further collaborations with them in this space Our new method for optimising recovery of proteomics samples will streamline proteomics workflows Our work on immune signalling is identifying potential targets for anti-inflammatory drug discovery (IKKalpha, FGFR) Our work on HSP90 inhibition is relevant to approaches to drug HSP90 as an approach to treat cancer Our work on PTPases is defining new modes of regulation and substrates for these enzymes which will inform new ways to drug them for a variety of indications |
| First Year Of Impact | 2018 |
| Sector | Education,Healthcare,Pharmaceuticals and Medical Biotechnology |
| Impact Types | Economic |
| Description | Babraham Institute Representative on Translational Research at EU Life, a consortium of EU Life Sciences Institutes |
| Geographic Reach | Europe |
| Policy Influence Type | Membership of a guideline committee |
| URL | http://eu-life.eu/ |
| Description | Alzheimer's Research UK Major Project Grant |
| Amount | £342,252 (GBP) |
| Funding ID | ARUK-PG2018B-021 |
| Organisation | Alzheimer's Research UK |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 09/2018 |
| End | 09/2022 |
| Description | BBSRC Industrial Partnership Award |
| Amount | £934,853 (GBP) |
| Organisation | AstraZeneca |
| Department | Astra Zeneca |
| Sector | Private |
| Country | United States |
| Start | 02/2019 |
| End | 02/2022 |
| Description | Babraham Institute SPOC Grant |
| Amount | £99,266 (GBP) |
| Organisation | Babraham Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 11/2020 |
| End | 11/2021 |
| Description | CASE Studentship |
| Amount | £99,034 (GBP) |
| Organisation | Eli Lilly & Company Ltd |
| Sector | Private |
| Country | United Kingdom |
| Start | 01/2019 |
| End | 01/2023 |
| Description | Characterisation of receptor tyrosine phosphatases in stem cells |
| Amount | £100,000 (GBP) |
| Organisation | Babraham Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 09/2020 |
| End | 09/2024 |
| Description | Disintegration of protein clearance pathways during ageing |
| Amount | £100,000 (GBP) |
| Organisation | Babraham Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 09/2020 |
| End | 09/2024 |
| Description | EMBO YIP |
| Amount | € 15,000 (EUR) |
| Organisation | European Molecular Biology Organisation |
| Sector | Charity/Non Profit |
| Country | Germany |
| Start | 01/2021 |
| End | 12/2024 |
| Description | Exploring the mechanism of the key signalling node, SHP2 |
| Amount | £100,000 (GBP) |
| Funding ID | 2502028 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2020 |
| End | 09/2024 |
| Description | Exploring the signalling mechanism of the tyrosine phosphatase SHP2 |
| Amount | £102,026 (GBP) |
| Funding ID | BB/V509310/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 08/2020 |
| End | 09/2024 |
| Description | Horserace Betting Levy Board Project Grant (as Co-PI with Professor Richard Piercy) |
| Amount | £230,535 (GBP) |
| Organisation | Horserace Betting Levy Board |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 05/2018 |
| End | 05/2020 |
| Description | Innovate UK |
| Amount | £770,434 (GBP) |
| Funding ID | Innovate UK |
| Organisation | PhoreMost |
| Sector | Private |
| Country | United Kingdom |
| Start | 04/2017 |
| End | 03/2019 |
| Description | Interrogating the function of deubiquitylases - BBSRC BRC CTP PhD studentship in collaboration with Mission Therapeutics |
| Amount | £1,320,000 (GBP) |
| Funding ID | BRC CTP Studentship award |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2022 |
| End | 09/2026 |
| Description | Investigating the targets and biological roles of the deubiquitylase USP43 |
| Amount | £348,246 (GBP) |
| Funding ID | BB/S017062/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 08/2019 |
| End | 08/2023 |
| Description | Lister Institute of Preventive Medicine Prize Fellowship |
| Amount | £250,000 (GBP) |
| Organisation | Lister Institute of Preventive Medicine |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 09/2020 |
| End | 09/2026 |
| Description | Novel substrates and functions for the DYRK protein kinases - BBSRC BRC CTP PhD studentship in collaboration with Cancer Research Horizons |
| Amount | £120,000 (GBP) |
| Organisation | Babraham Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 01/2024 |
| End | 12/2027 |
| Description | Outreach Award |
| Amount | $150,000 (USD) |
| Organisation | Thompson Family Foundation Initiative |
| Sector | Charity/Non Profit |
| Country | United States |
| Start | 09/2018 |
| End | 09/2020 |
| Description | Proteostasis modulation as a senescence-targeting strategy in ageing-associated diseases |
| Amount | £100,000 (GBP) |
| Organisation | Medical Research Council (MRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2021 |
| End | 09/2025 |
| Description | SPOC Synergy Grant |
| Amount | £158,917 (GBP) |
| Organisation | Babraham Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 01/2022 |
| End | 07/2023 |
| Description | Substrate profiling of the immune receptor CD45 |
| Amount | £85,000 (GBP) |
| Organisation | University of Cambridge |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 08/2019 |
| End | 02/2021 |
| Description | Unpicking the role of cellular stress responses in the efficacy of novel senolytic agents - BBSRC BRC CTP PhD studentship in collaboration with Phoremost |
| Amount | £100,000 (GBP) |
| Funding ID | BRC CTP Studentship Award |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 09/2022 |
| End | 09/2026 |
| Title | Anti-Afadin pY1230 |
| Description | We generated and validated a phosphospecific antibody against Afadin phosphorylated at amino acid Y1230. |
| Type Of Material | Antibody |
| Year Produced | 2022 |
| Provided To Others? | Yes |
| Impact | Unique tool for detection of this site. Enabled us to demonstrate substrate specificity of a phosphatase. |
| Title | BIM KO, BMF KO and BIM/BMF DKO CRISPR cell lines - A375 cells |
| Description | A375 lines with deletions of BIM, BMF or both BIM and BMF generated by CRISPR/Cas9 and validated |
| Type Of Material | Cell line |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | These cell lines have been used to define the mechanism of action of a novel drug combination that effectively kills melanoma cells Targeting melanoma's MCL1 bias unleashes the apoptotic potential of BRAF and ERK1/2 pathway inhibitors. Sale MJ, Minihane E, Monks NR, Gilley R, Richards FM, Schifferli KP, Andersen CL, Davies EJ, Vicente MA, Ozono E, Markovets A, Dry JR, Drew L, Flemington V, Proia T, Jodrell DI, Smith PD, Cook SJ. Nat Commun. 2019 Nov 14;10(1):5167. doi: 10.1038/s41467-019-12409-w |
| URL | https://pubmed.ncbi.nlm.nih.gov/31727888/ |
| Title | BIM KO, BMF KO and BIM/BMF DKO CRISPR cell lines - SW620 cells |
| Description | SW620 cell lines with deletions of BIM, BMF or both BIM and BMF generated by CRISPR/Cas9 and validated |
| Type Of Material | Cell line |
| Year Produced | 2020 |
| Provided To Others? | No |
| Impact | These cells have been used to define the mechanism of action of a novel drug combination that effectively kills colorectal cancer cells |
| Title | BIM KO, BMF KO and BIM/BMF DKO cell lines generated by CRISPR/Cas9 |
| Description | Human cell lines with CRISPR/Cas9-mediated gene deletion for BIM, BMF or both. Fully validated, KO confirmed. Defective for apoptosis arising from ERK1/2 inhibition |
| Type Of Material | Cell line |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | These have been used in a collaboration with AstraZeneca to test the pro-apoptotic efficacy of some of their drugs |
| Title | BIM/BMF DKO CRISPR cell lines - SK-MEL-30 |
| Description | SK-MEL-30 lines with deletions of both BIM and BMF generated by CRISPR/Cas9 and validated |
| Type Of Material | Cell line |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | These cell lines have been used to define the mechanism of action of a novel drug combination that effectively kills melanoma cells Targeting melanoma's MCL1 bias unleashes the apoptotic potential of BRAF and ERK1/2 pathway inhibitors. Sale MJ, Minihane E, Monks NR, Gilley R, Richards FM, Schifferli KP, Andersen CL, Davies EJ, Vicente MA, Ozono E, Markovets A, Dry JR, Drew L, Flemington V, Proia T, Jodrell DI, Smith PD, Cook SJ. Nat Commun. 2019 Nov 14;10(1):5167. doi: 10.1038/s41467-019-12409-w |
| URL | https://pubmed.ncbi.nlm.nih.gov/31727888/ |
| Title | CDKN1C/p57KIP2 KO cell lines |
| Description | We have used CRISPR/Cas9 gene editing to generate COLO205 cell line clones lacking the CDK inhibitor p57KIP2 encoded by CDKN1C |
| Type Of Material | Cell line |
| Provided To Others? | No |
| Impact | We have used these celsl to demonstrate the role of p57KIP2 in ERK-driven cell cycle arrest. This will form part of a future manuscript. Additional impacts will emerge as we undertake further analysis These will be made available to the community in the future |
| Title | Creation of PI3K avi-tag mice |
| Description | We introduced a 15 amino-acid Avi-tag into the C-terminus of endogenous genes encoding regulatory (p85alpha or p85beta) or catalytic (p110alpha, p110beta or p110delta) subunits of the Class IA PI3K family in mice. We also engineered expression of an optimised BirA (prokaryotic biotin ligase) expression cassette into the ROSA locus in mice and interbred these with the above Avi-tag mice. These mouse strains allow isolation of biotinylated Class IA PI3K subunits from mouse tissues and cell lines derived from them. |
| Type Of Material | Model of mechanisms or symptoms - mammalian in vivo |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | First publications: PMID: 30442661 |
| Title | DYRK1B and DYRK2 CRISPR KO cells lines |
| Description | HEK293 cells with CRISPR/Cas9-mediated deletion of DYRK1B or DYRK2 Fully sequenced and characterisation ongoing |
| Type Of Material | Cell line |
| Year Produced | 2019 |
| Provided To Others? | No |
| Impact | Initial characterisation has revealed some striking phenotypes that are currently being investigated. In the interim these cell lines are available for collaborators |
| Title | ERK1 or ERK2 CRISPR KO cells |
| Description | We have generated A375 and HCT116 cells which lack either ERK1 or ERK2 using CRISPR-Cas90 gene editing |
| Type Of Material | Cell line |
| Year Produced | 2022 |
| Provided To Others? | No |
| Impact | None yet. Research ongoing |
| Title | HEK293 cells stably expressing GFP-ATG13 and Histone 2B-mCherry |
| Description | HEK293 cells stably expressing GFP-ATG13 and Histone 2B-mCherry. These cells allow live cell imaging of the earliest stages of autophagosome synthesis (GFP-ATG13) during mitosis or interphase (H2B-mCherry) |
| Type Of Material | Cell line |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | These cells were part of a study that showed defintively that autophagy is repressed during mitosis An mTORC1-to-CDK1 Switch Maintains Autophagy Suppression during Mitosis. Odle RI, Walker SA, Oxley D, Kidger AM, Balmanno K, Gilley R, Okkenhaug H, Florey O, Ktistakis NT, Cook SJ. Mol Cell. 2020 Jan 16;77(2):228-240.e7. doi: 10.1016/j.molcel.2019.10.016 |
| URL | https://pubmed.ncbi.nlm.nih.gov/31733992/ |
| Title | Halo-TEV-TR-TUBE |
| Description | A trypsin-resistant tandem ubiquitin binding entity (TR-TUBE) with a HaloTag (for immobilization onto magnetic beads) and TEV protease cleavage site (for selective elution from beads). This reagent allows for stringent purification for poly-ubiquitylated proteins from biological samples. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2023 |
| Provided To Others? | No |
| Impact | TBD |
| Title | IKKalpha and IKKbeta KO cell lines |
| Description | We have generated HCT116 cell lines lacking one or other or both (DKO) of the critical NFkB activating protein kinases IKKalpha or IKKbeta using CRISPR/Cas9 gene targeting. In addition we have generated SW620 cell lines lacking either IKKalpha or IKKbeta using CRISPR/Cas9 gene targeting |
| Type Of Material | Cell line |
| Year Produced | 2022 |
| Provided To Others? | Yes |
| Impact | Too early for impacts; cells still being charatcerised. These will be made available to the community in the future |
| Title | Inducible DYRK1B cells |
| Description | HEK293 cells lines engineered to exhibit Tet-inducible expression of the DYRK1B protein kinase |
| Type Of Material | Cell line |
| Provided To Others? | No |
| Impact | New knowledge of the biological function of the DYRK1B protein kinase, including new substrates. New research papers New collaborations |
| Title | Inducible DYRK2 cell line |
| Description | HEK293 cells lines engineered to exhibit Tet-inducible expression of the DYRK2 protein kinase |
| Type Of Material | Cell line |
| Provided To Others? | No |
| Impact | New knowledge of DYRK2 Identification of new substrates of DYRK2 |
| Title | Mammalian expression plasmids for BIM mutants |
| Description | Mammalian expression plasmids encoding a variety of splice variants and mutants of the pro-apoptotic protein BIM, including phospho-site mutants |
| Type Of Material | Technology assay or reagent |
| Provided To Others? | Yes |
| Impact | New insights into the post-translational regulation of the pro-apoptptic protein BIM. |
| Title | Prex1 and Prex2 catalytically inactive mouse strains - Elpida Tsonou |
| Description | Elpida Tsonou has used CRISPR to generate knock-in mouse strains that render Prex1 and Prex2 Rac-GEFs catalytically inactive |
| Type Of Material | Model of mechanisms or symptoms - mammalian in vivo |
| Year Produced | 2019 |
| Provided To Others? | No |
| Impact | Publications expected within a couple of years |
| Title | SHP2 KO U2OS |
| Description | We have generated U2OS cells which lack SHP2 using CRISPR-Cas9 gene editing |
| Type Of Material | Cell line |
| Year Produced | 2022 |
| Provided To Others? | No |
| Impact | TBD |
| Title | SP4 Proteomics Sample Preparation Method |
| Description | New method that greatly simplifies proteomics sample preparation without the requirement for any specialist devices or reagents; performs as well as or better than current gold-standard approaches. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2021 |
| Provided To Others? | Yes |
| Impact | Multiple enquiries from both established and first-time proteomics researchers for advice on adapting the method for their experiments. |
| URL | https://www.biorxiv.org/content/10.1101/2021.09.24.461247v1 |
| Title | TagGFP2-NLS A549 cells |
| Description | A549 human lung epithelial cell line expressing green fluorescent protein tagGFP2 with a nuclear localisation sequence (NLS) for nuclear visualisation in live cells. |
| Type Of Material | Cell line |
| Year Produced | 2023 |
| Provided To Others? | No |
| Impact | TBD |
| Title | TagGFP2-NLS HBEC3-KT cells |
| Description | HBEC3-KT human lung epithelial cell line expressing green fluorescent protein tagGFP2 with a nuclear localisation sequence (NLS) for nuclear visualisation in live cells. |
| Type Of Material | Cell line |
| Year Produced | 2023 |
| Provided To Others? | No |
| Impact | TBD |
| Title | Tet-On::GFP-Huntingtin[Q97] A549 cells |
| Description | A549 human lung epithelial cell lines with doxycycline-inducible expression of GFP-tagged poly-glutamine-expanded Huntingtin (Htt[Q97]), a reporter of protein aggregate trafficking and clearance. |
| Type Of Material | Cell line |
| Year Produced | 2023 |
| Provided To Others? | No |
| Impact | TBD |
| Title | Tet-On::HaloTag-Huntingtin[Q25] A549 cells |
| Description | A549 human lung epithelial cell lines with doxycycline-inducible expression of HaloTagged wild-type Huntingtin (Htt[Q25]), a control reporter of protein aggregate trafficking and clearance. |
| Type Of Material | Cell line |
| Year Produced | 2023 |
| Provided To Others? | No |
| Impact | TBD |
| Title | Tet-On::HaloTag-Huntingtin[Q97] A549 cells |
| Description | A549 human lung epithelial cell lines with doxycycline-inducible expression of HaloTagged poly-glutamine-expanded Huntingtin (Htt[Q97]), a reporter of protein aggregate trafficking and clearance. |
| Type Of Material | Cell line |
| Year Produced | 2023 |
| Provided To Others? | No |
| Impact | TBD |
| Title | Tet-On:GFP-Huntingtin[Q25] A549 cells |
| Description | A549 human lung epithelial cell lines with doxycycline-inducible expression of GFP-tagged wild-type Huntingtin (Htt[Q25]), a control reporter of protein aggregate trafficking and clearance. |
| Type Of Material | Cell line |
| Year Produced | 2023 |
| Provided To Others? | No |
| Impact | TBD |
| Title | USP43 KO cell lines |
| Description | HCT116 and A375 cell lines with deletion of USP43 generated by CRISPR/Cas9 gene editing |
| Type Of Material | Cell line |
| Year Produced | 2019 |
| Provided To Others? | No |
| Impact | Cell lines currently being characterised and alreayd revelaing some interesting phenotypes. These will be available for use by collaborators prior to publication |
| Title | ZFP36 KO cell llines |
| Description | We have generated HCT116 cell liens in which we have deleted the RNA binding proteins ZFP36L1 or ZFP36L2 - singly or in combination (DKO) using CRISPR/Cas9 gene editing. |
| Type Of Material | Cell line |
| Provided To Others? | No |
| Impact | ZFP36L1 KO cells featured in our 2016 manuscript - Galloway et al Science Further impacts will arise as we undertake further characterisation of these cell lines These will be made available to the community in the future |
| Title | hTERT-immortalised Human Coronary Artery Endothelial Cells |
| Description | Primary human coronary artery endothelial cells (obtained commercially) immortalised with hTERT (telomerase subunit) |
| Type Of Material | Cell line |
| Year Produced | 2023 |
| Provided To Others? | No |
| Impact | TBD |
| Title | ipsc autophagy deletion |
| Description | Generated ips cells deleted for the autophagy gene ATG13 |
| Type Of Material | Cell line |
| Year Produced | 2023 |
| Provided To Others? | Yes |
| Impact | Useful reagent for screening autophagy inducing compounds |
| Title | mKate2-NLS A549 cells |
| Description | A549 human lung epithelial cell line expressing red fluorescent protein mKate2 with a nuclear localisation sequence (NLS) for nuclear visualisation in live cells. |
| Type Of Material | Cell line |
| Year Produced | 2023 |
| Provided To Others? | No |
| Impact | TBD |
| Title | mKate2-NLS HBEC3-KT cells |
| Description | HBEC3-KT human lung epithelial cell line expressing red fluorescent protein mKate2 with a nuclear localisation sequence (NLS) for nuclear visualisation in live cells. |
| Type Of Material | Cell line |
| Year Produced | 2023 |
| Provided To Others? | No |
| Impact | TBD |
| Title | DUB RNAi screen |
| Description | In collaboration with MISSION Therapeutics we have undertaken an RNAi screen with human deubiquitylating enzymes (DUBs) to identify those DUBs that confer protection against ERK pathway, mTOR inhibition or PERK inhibition Datat is currently being analysed before candidates are selected for further characterisation |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | Too early for impacts This data will be made available to the community in the future |
| Title | DYRK1B phosphoproteomics data set |
| Description | Using HEK293 cells exhibiting inducible expression of the DYRK1B protein kinase (HD1B cells) we have performed Phospho-SILAC mess spectrometry to identify DYRK1B-inducible phosphoproteins. Some of these turn out to be direct DYRK1B substrates |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | This data set has allowed us to identify new DYRK1B substrates and so has provided new insights into the function of this protein kinases in controlling gene expression and autophagy. The dataset will ultimately be released and freely available when the first mansucript is published |
| Title | DYRK1B transcriptome gene data set |
| Description | RNA-seq data set derived from HEK293 cells which exhibit Tet-regulated DYRK1B expression Reports genome wide changes in abundance and splicing patterns in response to DYRK1B expression |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | Too soon for impacts. Data currently being analsyed |
| Title | DYRK2 Transcriptome gene data set |
| Description | RNA-seq data set derived from HEK293 cells which exhibit Tet-regulated DYRK2 expression Reports genome wide changes in abundance and splicing patterns in response to DYRK2 expression |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | Too soon for impacts. Data currently being analysed |
| Title | DYRK2 phosphoproteomics dataset |
| Description | Using HEK293 cells exhibiting inducible expression of the DYRK2 protein kinase (HD2 cells) we have performed Phospho-SILAC mess spectrometry to identify DYRK2-inducible phosphoproteins. Some of these turn out to be direct DYRK2 substrates |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | This dataset has allowed us to identify DYRK2-inducible phosphoproteins, including new substrates, providing new insghts into the function of the DYRK2 protein in gene expression, autophagy/proteostasis and cell motility |
| Title | ERK pathway cell cycle arrest/senescence gene data set |
| Description | Human Illumina bead array describing changes in transcriptome associated with ERK-driven cell cycle arrest/senescence |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | New insights into ERK signalling associated with cell cycle arrest and senescence Identification of new links between ERK signalling the cell cycle Identification of ERK target genes |
| Title | IKKa, IKKb and IKK DKO gene sets |
| Description | RNAseq data sets from human colorectal epithelial cell lines in which IKKa, IKKb or both IKKs have been deleted by CRISPR/Cas9. Both basal and TNFalpha-induced conditions were employed so that we can define the contribution of IKKs to basal and cytokine-induced gene expression. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | The IKKs are the kinases that drive activation of the NFkappaB (NFkB) transcription factor. This is the first instance of knockout of the IKKs in human cells. We have confirmed the expression of known NFkB target genes but also identified an array of novel IKK-dependent genes that are candidate NFkB gene targets. Some of these have roles in autophagy, protein turnover, protein trafficking, cell motility and inflammation. This data will be made available freely when the first manuscript is published. |
| Title | SEC-MS of the HSP90 inhibitor-modulated proteome in HT29 human colon cells |
| Description | Web-based proteomics resource to explore the global native protein complex elution profiles in the HT29 human colon adenocarcinoma cell line, and how this is remodelled by the clinically-relevant HSP90 inhibitor tanespimycin (17-AAG). This point-and-click database allows researchers without experience in proteomics data analysis to explore and visualise the individual protein elution profiles for 4,645 proteins, with and without HSP90 inhibition. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2022 |
| Provided To Others? | Yes |
| Impact | The analysis tools and methodologies used to generate this dataset and database are being used for several multi-dimensional proteomics experiments in our group, and more broadly at Babraham Institute. |
| URL | https://www.bioinformatics.babraham.ac.uk/shiny/HSP90/SEC-MS/ |
| Description | C. O'Neill- PINK |
| Organisation | University College Cork |
| Department | School of Biochemistry and Cell Biology |
| Country | Ireland |
| Sector | Academic/University |
| PI Contribution | We provided advice and measured PIP3 in samples provided by the partner |
| Collaborator Contribution | Access to unpublished data |
| Impact | Furlong etal 2019. |
| Start Year | 2018 |
| Description | Collaboration on mechanism and prevention of Wallerian degeneration |
| Organisation | AstraZeneca |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Basic research into activation mechanism of SARM1 |
| Collaborator Contribution | Industrial partnership as part of BBSRC IPA award. Contributes knowledge, discussion, research collaboration, specialised methods such as Mass Spec and chemical synthesis. |
| Impact | BBSRC IPA Award |
| Start Year | 2017 |
| Description | Collaboration on new targets of neurodegeneration involving autophagy |
| Organisation | University of Cambridge |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Identify targets using cell biological assays |
| Collaborator Contribution | Provide relevant assays |
| Impact | not yet |
| Start Year | 2021 |
| Description | Dario Alessi - SGK |
| Organisation | University of Dundee |
| Department | MRC Protein Phosphorylation and Ubiquitylation Unit |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We provided advice and measured PIP3 in samples provided by partner. |
| Collaborator Contribution | Access to unpublished data relevant to project |
| Impact | Multidisciplinary, mass spectrometry, genetics, cancer, cell biology. |
| Start Year | 2018 |
| Description | David Adams |
| Organisation | The Wellcome Trust Sanger Institute |
| Department | Human Genetics |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Measurements of phosphoinositdes and biochemical analysis of signalling, interpretation of data |
| Collaborator Contribution | Genome wide screen for genes essential for cancer progression |
| Impact | Building collaboration looking at role of cds2 in rare subset of cancers. |
| Start Year | 2021 |
| Description | Developing novel cell based assays to find new inhibitors the RAS-RAF-MEK-ERK pathway |
| Organisation | PhoreMost |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | We have developed cell-based transcriptional reporter assays that allow screening in cells for novel inhibitors of the RAS-RAF-MEK-ERK signalling pathways |
| Collaborator Contribution | Our partners have sued these cell based assays to screen for novel peptide-based inhibitors using their proprietary technology |
| Impact | This collaboration led to a successful Innovate UK funding award between PhoreMost and the Cook lab at the Babraham Institute It has also led to a separate 3-way research collaboration between PhoreMost, the Cook lab at the Babraham Institute and Plexxikon, a structure-based drug discovery SME in California |
| Start Year | 2017 |
| Description | Eli-Lilly |
| Organisation | Eli Lilly & Company Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | We have delivered research on a Case award PhD studentship that is relevant to the objectives of the company. |
| Collaborator Contribution | Funding the research |
| Impact | Publications in the future. |
| Start Year | 2016 |
| Description | Giuseppe Orsomando |
| Organisation | Marche Polytechnic University |
| Country | Italy |
| Sector | Academic/University |
| PI Contribution | Expertise and material from axon degeneration models |
| Collaborator Contribution | Expertise in measurement of NAD-related metabolites and enzyme assays |
| Impact | Carpi, F.M., Cortese, M., Orsomando, G., Polzonetti, V., Vincenzetti, S., Moreschini, B., Coleman, M.P., and Magni, G. (2018). Simultaneous quantification of nicotinamide mononucleotide and related pyridine compounds in mouse tissues by UHPLC-MS/MS. Sep Sci plus. 1(1):22-30. -- Di Stefano, M., Loreto A., Orsomando, G., Mori V., Zamporlini, F., Hulse, R.P., Webster, J., Donaldson, L.F., Gering, M., Raffaelli, N., Coleman, M.P., Gilley, J., and Conforti L. (2017). NMN deamidase delays Wallerian degeneration and rescues axonal defects caused by NMNAT2 deficiency in vivo. Current Biology. 27(6):784-794 |
| Start Year | 2016 |
| Description | Hosted Erasmus students from Greece and Turkey |
| Organisation | Agricultural University of Athens (AUA) |
| Country | Greece |
| Sector | Academic/University |
| PI Contribution | The students visited the lab to gain experience on cell biology techniques and to continue using those techniques in their country as a collaboration |
| Collaborator Contribution | Collaborative work |
| Impact | Thesis work by the students in their country |
| Start Year | 2021 |
| Description | Linda Partridge |
| Organisation | University College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We contributed to the planning of mouse ageing experiments. We have analysed samples generated in the collaborators lab using lipidomic techniques. |
| Collaborator Contribution | They housed ageing mice and generated samples fo various tissues. |
| Impact | Publications, eg Hahn etal 2017, listed in our outputs. |
| Start Year | 2015 |
| Description | Lloyd Trotmann |
| Organisation | Cold Spring Harbor Laboratory (CSHL) |
| Department | Cancer Centre |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | We have performed proteomic and cellular studies of PTEN deficient mouse prostate tissue |
| Collaborator Contribution | They performed PET imaging of glucose uptake into mouse prostate tissues control and cancer models |
| Impact | Shared results and discussion about further work |
| Start Year | 2018 |
| Description | Novel targets of autophagy using structural information and AI |
| Organisation | University of Cambridge |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | A collaboration with two academic labs in the UK and Australia, together with a biotechnology company in Singapore in order to identify and characterize small molecules that activate selective autophagy against specific targets. My contribution thus far is in identifying ways to select targets, and to select the autophagy machinery that needs to be activated. |
| Collaborator Contribution | The academic partners provide additional information on target selection and validation. The commercial partners provide AI support and chemical synthesis capability. |
| Impact | We are in early steps of discussions and target selection |
| Start Year | 2021 |
| Description | Paradoxical effects on RAF inhibitors on cell proliferation and viability |
| Organisation | University of Liverpool |
| Department | Institute of Integrative Biology |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We have dicovered that a range of RAF kinase inhibitors drive cell cycle arrest and even cell death that is independent of their ability to regulate activity of the RAF-MEK-ERK signalling pathway. We are seeking to understand how they do this. Is it an 'on target' effect of RAF inhibtion or does it reflect activity against another target? |
| Collaborator Contribution | Our collaborators are experts in kinase enzymology with unique expertise in biochemsitry and biophysics. They will monitor kinase inhibtor-protein interactions and also interrogate binding to potential candidates by in silico analysis. |
| Impact | Too early for any tangible outcomes |
| Start Year | 2020 |
| Description | Partnerhsip in Ageing Research - BI and MPI-AGE |
| Organisation | Max Planck Society |
| Department | Max Planck Institute for the Biology of Ageing |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | This Global Partnering Award was designed to support a visit by BI staff to the Max Planck Institute for Biology of Ageing (MPI AGE) in Cologne. This was an opportunity for BI colleagues to meet scientists at the MPI AGE, spend two days discussing shared research interest and to identify areas for tangible research collaborations. |
| Collaborator Contribution | The meeting between BI and MPI-AGE identified three research areas where we wanted to support collaborations through the award of pump priming grants. Ina Huppertz (MPI- AGE) & Ian McGough (BI) - examining the impact of age on mRNA modifications (8oxoG and ADP-ribosylation) Jon Houseley (BI) & Ron Jachimowicz (MPI AGE) - Epigenetic ageing clock and cancer Rahul Samant (BI) & Kostas Demetriades (MPI AGE) - Role of Ubiquitination in mTOR function. Each Institute (BI and MPI AGE) contributed funds to support these three projects |
| Impact | Too early to say yet. These are pump-priming projects that have only just started. |
| Start Year | 2023 |
| Description | Peter Parker PKB kinases |
| Organisation | Francis Crick Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We have discussed a project in the Parker lab involving the potential for kinases , other than PDK1 , to be phosphorylating T308 in PKB. We supplied advice over assays and purified recombinant PKB from SF9 cells to enable them to conduct assays based on those we have published. |
| Collaborator Contribution | Provided us with access to their unpublished data on the subject of regulation of PKB. |
| Impact | Multi-disciplinary involving structural biology , biochemistry, cell biology, modelling and biophysics. |
| Start Year | 2020 |
| Description | Prof Richard Piercy |
| Organisation | Royal Veterinary College (RVC) |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Expertise in measuring axonal transport |
| Collaborator Contribution | Expertise in equine veterinary science; nerve samples from horses |
| Impact | Dr Robert Adalbert will start a two-year collaboration project with Prof Richard Piercy's group in RVC. |
| Start Year | 2017 |
| Description | Professor Richard Ribchester |
| Organisation | University of Edinburgh |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Characterisation of Sarm1/Nmnat-2 double knockout mice with axonal and neuromuscular synaptic protection. |
| Collaborator Contribution | Professor Richard Ribchester and his group contributed experimental data derived from muscle tension recordings from knockout mice with axonal and synaptic protection. |
| Impact | Gilley, J., Ribchester, R.R., and Coleman, M.P. (2017). Sarm1 deletion, but not WldS, confers lifelong rescue in a mouse model of severe axonopathy. Cell Reports. 21(1):10-16 |
| Start Year | 2016 |
| Description | Small molecule activators of the ISR - Leeds |
| Organisation | University of Leeds |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We have identified small molecule activators of the ISR, their likely mechanism of action and their likely molecular target |
| Collaborator Contribution | Prof Richard Bayliss has constructed models of the small molecules binding to the proposed target based on available crystal structures |
| Impact | A manuscript is in preparation |
| Start Year | 2023 |
| Description | Small molecule activators of the Integrated Stress Response (ISR) - Liverpool |
| Organisation | University of Liverpool |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We have identified novel small molecule activators of the Integrated Stress Response (ISR), a pathway involved in cellular health and longevity. Patrick Eyers and Niall Kenneth have provided expertise and conducted experiments to identify the mechanism by which these molecules active the ISR |
| Collaborator Contribution | Intellectual - suggesting possible targets Practical - performing experiments |
| Impact | A manuscript is in preparation |
| Start Year | 2023 |
| Description | TAK-CELERATOR |
| Organisation | Takeda Cambridge Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Delivered research on neuronal loss that is in line with company objectives |
| Collaborator Contribution | Funded the research |
| Impact | Leading towards publications |
| Start Year | 2017 |
| Description | Takeda |
| Organisation | Takeda Cambridge Ltd |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Our team has performed research on the pathways controlling neuronal-loss that is relevant to their objectives |
| Collaborator Contribution | Funded work in the lab |
| Impact | Publications reported in outputs. |
| Start Year | 2012 |
| Description | Valerie O Donnell |
| Organisation | Cardiff University |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Analysis and identification of phosphoinositides |
| Collaborator Contribution | Lipidomics and data and analysis |
| Impact | Submission of grant application to support further work at including Babraham, Cardiff |
| Start Year | 2021 |
| Description | Vernalis Research Cambridge |
| Organisation | Vernalis |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Wrote iCase studentship application, recruited joint PhD student Elizabeth Hampson, supervision of student and carrying out of project |
| Collaborator Contribution | Provision of time and expertise and industrial placement, |
| Impact | Recently established collaboration, no outputs yet |
| Start Year | 2017 |
| Description | "What's My Line?" School Careers Event - Yasmeen Al-Mufti |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | 180 sixth form aged students attended this event where staff discussed and answered questions about what a bioscience career is like as well as discussing research interests. Teachers reported that students had increased their knowledge of such careers and especially highlighted the breadth of different roles the event highlighted to them. |
| Year(s) Of Engagement Activity | 2021 |
| Description | 6th Form Conference |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | Further education students present research posters which are judged by researcher staff following conversations around their work. Presentation on research and career journey was given to all students will a Q&A following |
| Year(s) Of Engagement Activity | 2022 |
| URL | https://www.babraham.ac.uk/events/2022/07/sixth-form-conference-2022-healthy-ageing |
| Description | ARUK corporate partnership lay talk - Dr Claire Durrant |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Industry/Business |
| Results and Impact | Visit and presentation to Cambridge Cognition with the ARUK regional fundraiser. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Ageing research working group |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Policymakers/politicians |
| Results and Impact | working group aiming to define the physiological society's policy on research into ageing and the societal consequences. This was used to inform the Department of Health and the public. |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | Babraham Institute Schools Day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | At Schools' Day, students from schools all over Cambridgeshire and beyond gather at the Institute to discover more about our world-leading bioscience research. Led by Institute researchers, secondary and sixth-form students complete hands-on lab projects. Often using equipment not available within schools, Schools' Day aims to enthuse, inspire and motivate students and provide insight into what life is really like in the lab. Schools' Day is for students in Years 10-13. Typically, up to 5 students per school or sixth form may attend, although more places may be offered if capacity allows. There is active outreach done to promote the opportunity to schools and students from areas of high deprivation and an associated travel bursary to enable this targeted audience approach. |
| Year(s) Of Engagement Activity | 2023 |
| URL | https://www.babraham.ac.uk/blog/SchoolsDay-2023 |
| Description | Cambridge Science Festival , David Barneda |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Cambridge science festival presentation/ discussion on the role of science in society |
| Year(s) Of Engagement Activity | 2019 |
| Description | Cambridge Science Festival Escape room |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | We ran an escape room experience as part fo the wider Cambridge Science festival event. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Cell scientist to watch |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Profile of career and research as a 'Cell scientist to watch' by the Journal of Cell Science. |
| Year(s) Of Engagement Activity | 2020 |
| Description | Comberton Village college AA, TC and PTH |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | A carrels in bioscience discussion involving a group leader, a post doc and a PhD student from our lab. They meet with a yr 12 (17yrs old) cohort at Comberton VC a local school. |
| Year(s) Of Engagement Activity | 2022 |
| Description | Comment to daily mail |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Public/other audiences |
| Results and Impact | "As Nicholas Ktistakis explains: 'When we go into periods of fasting, we force the autophagy system to go into overdrive to break down parts of the cell to make energy. But this also starts to burn through all the detritus and eliminate bad things that make the cell less effective. 'Some scientists who work in this area follow a 5:2 diet, where you fast for two days a week. Or for a similar effect, cut back to 40 per cent of what you normally eat for two weeks every once in a while. I try this two-week reduced intake every few months.' Excerpt from the article where Nicholas Ktistakis spoke to Thea Jourdan." |
| Year(s) Of Engagement Activity | 2022 |
| URL | https://www.dailymail.co.uk/health/article-10461405/The-simple-scientific-daily-tweaks-leading-scien... |
| Description | Conference participation, HW lab, Small GTPases meeting Biochem Soc 2018: 1 session chair, 1 invited talk, 4 talks selected from abstracts |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Our lab was heavily represented at this outstanding international meeting. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Escape Room Installation |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | A 'Signalling' Escape Room was designed by students within the Signallign Laboratory, working with the Public Engagement team. This was then presented by studnets and post-docs, including members of the Cook lab at both the Cambridge Science Festival and the Latitude Music Festival. |
| Year(s) Of Engagement Activity | 2019,2020 |
| Description | Evaluation of PhD Programme, Vienna Biocenter |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Evaluated the PhD programme of the Vienna Biomecal to decide on further funding for the next 5 years |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://training.vbc.ac.at/phd-program/ |
| Description | Evaluation of three Biology Departments in Greece (Ioannina, Larissa, Thessaloniki) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Part of a 3 member panel evaluating for one week each of those Departmemts so they can continue to provide Undergraduate and Graduate Degrees |
| Year(s) Of Engagement Activity | 2019,2020 |
| Description | Expert opinion for news article (online) - Harvey Johnston |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Responded to a journalist request for expert opinion on an article on technological advances in the field. Several opinions and quotes included in the published article (alongside more senior experts in the field). |
| Year(s) Of Engagement Activity | 2022 |
| URL | https://www.technologynetworks.com/proteomics/articles/advances-in-proteomics-358809 |
| Description | Expert opinion on DeepMind press release/article |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Provided expert comments and perspective to a journalist writing an article on a specific breaking news story (an Artificial Intelligence system solving a long-standing scientific challenge in my field). Some of these quotes appeared in the released article (on the digital magazine 'UnHerd'). The journalist has requested I keep in touch for other developments in my field. |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://unherd.com/2020/12/how-deepmind-is-transforming-the-future/ |
| Description | Featured in ASBMB conference preview: New kids on the signaling block |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Highlighted as an upcoming researcher in the signaling field. https://www.asbmb.org/asbmb-today/science/092721/new-kids-on-the-signaling-block |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://www.asbmb.org/asbmb-today/science/092721/new-kids-on-the-signaling-block |
| Description | How to read research papers |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | Discussion with Bristol university PhD studetns about reading and analysing research papers. |
| Year(s) Of Engagement Activity | 2018 |
| Description | In conversation with the Babraham Institute - part of Cambridge Science Festival |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | 1:1 dialogue with general public (open invitation but registration required for numbers). Evening reception in which we explain our science and answer questions Part of a programme of events for the annual Cambridge Science Festival |
| Year(s) Of Engagement Activity | 2017 |
| Description | Launchpad filming |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Part of the filming was at Babraham |
| Year(s) Of Engagement Activity | 2018 |
| Description | Leader of discussion about running a successful research team with BBSRC fellows. |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Discussion with BBSRC fellows about issues inovled in running research teams, strengths, weaknesses, mistakes, successes. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Lecture in Cancer Biology and Medicine training course |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Postgraduate students |
| Results and Impact | I presented a lecture on how signalling pathways are remodelled to drive innate or acquired resistance to new targeted anti-cancer agents that are clincially approved or in development. The lecturer reached approx 60 Master and PhD students, clinicians some patient advocates and charity-funded researchers. There was a vibrant follow-up Q&A session and new contacts were established with the prospect of future collaborations. In feedback >80% of the audience found it useful |
| Year(s) Of Engagement Activity | 2020,2021 |
| Description | MRC LMB alumni interview |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Profile of career and research as a former PhD student in MRC LMB alumni magazine. |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://www2.mrc-lmb.cam.ac.uk/about-lmb/lmb-alumni/share-your-memories/hayley-sharpe-looking-back/ |
| Description | Meet a Bioscientist |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Virtual research and career talk with extended Q&A |
| Year(s) Of Engagement Activity | 2022 |
| Description | Meet a Bioscientist |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | Virtual research and career talk with extended Q&A |
| Year(s) Of Engagement Activity | 2022 |
| URL | https://www.babraham.ac.uk/blog/meet-a-bioscientist-2022 |
| Description | Member of the Science Advisry Board of PhoreMost |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Industry/Business |
| Results and Impact | Offer advice and insight into drug discovery prpocess for PhoreMost, an SME based on the Babraham Research Campus |
| Year(s) Of Engagement Activity | 2020,2021 |
| URL | https://www.phoremost.com |
| Description | News article about new proteomics method |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Coverage of a new proteomics method following press release 'New technique recovers more 'hard-to-get' proteins'. |
| Year(s) Of Engagement Activity | 2022 |
| URL | https://www.genengnews.com/topics/omics/proteomics-sample-prep-upgrade-captures-more-proteins-for-le... |
| Description | News article covering HSP90 research |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | News article on Institute website about Samant lab research 'A new approach gives a better view of the proteomic fall-out of HSP90 inhibition'. |
| Year(s) Of Engagement Activity | 2023 |
| URL | https://www.babraham.ac.uk/news/2023/01/new-approach-gives-better-view-proteomic-fall-out-hsp90-inhi... |
| Description | Participated in Babraham Institute exhibit at Royal Society Summer Science Exhibition |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | The Babraham Institute prepared an exhibit - The Ageing Clock - which exemplified aspects of our ageing research portfolio for a public audience. Tjis was selcted tp be part of the prestigious Royal Society Summer Science Exhibition and I was involved in presentign this exhbit to the Public togehter with colleagues. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Participation in Public Engagement event on genome editing - part of the Cambridge Science Festival |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | A debate/dialgoue open to the pulbic entitled 'Genome editing: How far should we go' |
| Year(s) Of Engagement Activity | 2018 |
| Description | Patient and Public Involvement in Research Workshop - Dr Andrea Loreto |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | Training session organised by Parkinson's UK for Parkinson's researchers. The training session is open for all Parkinson's researchers - across every discipline, even those in the laboratory working on animal and cell cultures. We want the researchers we fund to involve the perspective of people affected by Parkinson's in their research as much as possible, and while it is certainly more difficult to see its applications in non-clinical research, we have evidence of the important role it can play. In the training we will be working through examples of involvement in clinical and lab-based research and the content will be applicable to both. Within the context of laboratory based research, the purpose of involvement might be to: • Help researchers communicate the findings and progress of a project - including reviewing presentations, progress reports or press releases and promoting events • Motivate researchers who may have no direct contact with patients and help researchers to take account of patient experience • Help to review findings and identify areas for future study • Legitimise funding decisions, thereby reassuring potential donors |
| Year(s) Of Engagement Activity | 2017 |
| Description | Poster Judge at Sixth-Form Conference |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Acted as a poster judge for Sixth Form Conference that was held at Babraham Institute. Engaged with 10-15 students, as well as their parents and teachers. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Poster presentation at Parkinson's Open-day (Saturday 4th November 2017) |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Patients, carers and/or patient groups |
| Results and Impact | Dr Andrea Loreto and Prof Coleman poster presentation to Parkinson's patients during the open day organised by Prof Roger Barker's Clinic at the John van Geest Centre for Brain Repair. Poster title: Preventing axon loss caused by mitochondrial dysfunction in Parkinson's disease |
| Year(s) Of Engagement Activity | 2017 |
| Description | Production of research video |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Produced an animated video overview of current and ongoing research around PI3K and wider cell signalling. Video published on Institute youtube account and promoted to public audiences as well as other sector stakeholders. |
| Year(s) Of Engagement Activity | 2021 |
| Description | Protein challenge , Simon Rudge |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | hands on lab work. |
| Year(s) Of Engagement Activity | 2021 |
| Description | Proteostasis: From organelles to organisms |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Gave 15 minute talk (selected from submitted abstract) on my research. Sparked 2 new collaborations; invitation to write a review article on my field (since published in peer-reviewed journal); invitation to be an expert peer-reviewer for a journal; several participiants applied to future advertised vacancies in my group. |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://meetings.embo.org/event/19-proteostasis |
| Description | Research Access Programme Student Placement |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Undergraduate students |
| Results and Impact | Higher education student carried out 5 week online summer placement learning a variety of techniques and data analysis methods. They reported an increase in knowledge, skills, and a broadening of their understanding of careers in science as well as how academic research functions |
| Year(s) Of Engagement Activity | 2021 |
| Description | Research Access Programme Student Placement |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Undergraduate students |
| Results and Impact | Higher education student carried out 5 week online summer placement learning a variety of techniques and data analysis methods. They reported an increase in knowledge, skills, and a broadening of their understanding of careers in science as well as how academic research functions. |
| Year(s) Of Engagement Activity | 2021 |
| URL | https://www.babraham.ac.uk/about-us/impact/public/research-access-programme |
| Description | Research Experience |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | A work experience day for an A-level biology student from a local school. The student undertook hands on practicals to gain lab experience and also explored research of the lab. The student also spent some time with the imaging facility to gain insight into how that facility of the Institute functions |
| Year(s) Of Engagement Activity | 2023 |
| Description | Research talk for 6th Form Students |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Gave a research talk to 6th Form students covering research and career route, as well as, introducing the students to the practical experiments they would be undertaking as part of the Protein Challenge project. This was part of the Protein Challenge project where students undertake a 6 week research project themed around Institute research. They learn a number of practical scientific methods as well as gaining valuable transferable skills on how to present and communicate science to audiences |
| Year(s) Of Engagement Activity | 2023 |
| Description | Review of Research Unit "Mechanisms of Lysosomal Homeostasis", Germany |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Evaluated a Research Consortium composed of several laboratories in Germany applying to DFG (the major funder in Germany) for a multiyear grant involving 10 laboratories |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://for2625-lysosomes.de/ |
| Description | Royal society summer exhibition |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Exhibits interactively presenting the work in the signalling programme where presented at the Royal Soc, audience of approx 10000 over 7 days |
| Year(s) Of Engagement Activity | 2018 |
| Description | Samual Ward College Careers Fair |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Discussed science careers and cell signalling research with key stage 3 , 4 and 5 students. Used interactives to spark conversations around research, as well as, pipetting activity to introduce students to lab based skills |
| Year(s) Of Engagement Activity | 2022 |
| Description | Sawston School student work experience |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | One week work experience placement for 6th form aged student. Engaged with lab research, the wider Institute and science careers |
| Year(s) Of Engagement Activity | 2022 |
| Description | School visit |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | "At Schools' Day, students from schools all over Cambridgeshire and beyond gather at the Institute to discover more about our world-leading bioscience research. Led by Institute researchers, secondary and sixth-form students complete hands-on lab projects. Often using equipment not available within schools, Schools' Day aims to enthuse, inspire and motivate students and provide insight into what life is really like in the lab. Schools' Day is for students in Years 10-13. Typically, up to 5 students per school or sixth form may attend, although more places may be offered if capacity allows. There is active outreach done to promote the opportunity to schools and students from areas of high deprivation and an associated travel bursary to enable this targeted audience approach." |
| Year(s) Of Engagement Activity | 2023 |
| URL | https://www.babraham.ac.uk/blog/SchoolsDay-2023 |
| Description | School visits |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | The students were enthused about the topic of my presentation and this led to dialogue and discussion about several issues including new cancer therapies, evolution of drug resistance in cancer, the use of animals in research. Anecdotally, the institute received requests for summer placement students following this visit. |
| Year(s) Of Engagement Activity | 2014,2015,2016,2017,2018,2019 |
| Description | Schools Day for Teachers |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Gave a 15 min talk on my group's research interests to a group of secondary school teachers. |
| Year(s) Of Engagement Activity | 2019 |
| URL | https://www.babraham.ac.uk/news/2019/11/schools-day-for-teachers |
| Description | Schools day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | 125 pupils attended schools day, talks given to all and lab practicals ran for two small groups. |
| Year(s) Of Engagement Activity | 2018,2019 |
| Description | Schools day Feb 2018 Babraham Institute |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Discussion, question/answer and experiment to show how neutrophils contribute to immune defence and a broader dialogue about the societal signifcance of research and the types of jobs that can be involved. |
| Year(s) Of Engagement Activity | 2014,2015,2016,2017,2018 |
| Description | Schools talk and Q&A |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | Virtual research and career talk with extended Q&A |
| Year(s) Of Engagement Activity | 2022 |
| URL | https://www.babraham.ac.uk/blog/meet-a-bioscientist-2022 |
| Description | Science Open Day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | Students visited the lab and undertook small lab-based proejcts supervised by students/post-docs and myself. I explained the research that we do and discussed ethical issues such as the use of animals in research. This precipitated excellent discussion and dialogue. We received excellent feedback from the schools involved and requests for further outreach activities |
| Year(s) Of Engagement Activity | 2013,2014,2015,2016,2017,2018,2019,2020 |
| Description | Social Mobility Foundation Student Mentoring |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | Mentoring provided to a sixth form student from an area of low social mobility through the Social Mobility Foundation's mentoring scheme. Conversations were had around career route and research interests to inform and inspire. |
| Year(s) Of Engagement Activity | 2020,2021 |
| Description | Talk at Hills Road Sixth Form College, Cambridge |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Presentation to 40 6th form students. Title: The nervous system: the life and death of cells inside your head. |
| Year(s) Of Engagement Activity | 2018 |
| Description | University of Michigan Protein Folding Diseases Seminar Series |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | gave a talk on my research |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://vimeo.com/477740122 |
| Description | Visits by Teachers |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | My lab has hosted 6th form Biology teachers who were visiting my Institution during Half Term to update their knowledge as part of their CPD |
| Year(s) Of Engagement Activity | 2016,2017,2018,2019,2020 |
| Description | science spotlight public talk |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | On line presentation targeted to adult community groups in an area of deprivation |
| Year(s) Of Engagement Activity | 2021 |
| Description | student poster |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Facilitated a poster presentation session where students from a local 6th Form college presented scientific posters communicating a research project they had undertaken. This involved discussing research with students and linking to Institute research as well as providing feedback on scientific poster design. This was part of the Protein Challenge project where students undertake a 6 week research project themed around Institute research. They learn a number of practical scientific methods as well as gaining valuable transferable skills on how to present and communicate science to audiences |
| Year(s) Of Engagement Activity | 2023 |