Studentship: Investigation into the effect of spike glycoprotein glycosylation for rational vaccine development

Lead Research Organisation: The Pirbright Institute
Department Name: UNLISTED

Abstract

Infectious bronchitis virus (IBV) has four structural proteins; spike (S) glycoprotein, membrane (M) and envelope (E) proteins which are found in the lipid membrane surrounding the virus particle and the nucleocapsid (N) protein binds the positive-sense RNA genome. The S glycoprotein is highly glycosylated with N-linked high-mannose type oligosaccharides (Cavanagh et al., 1983) and mediates attachment of the virus to host cells and fusion of virus-cell and cell-cell. Previous work has shown that exchanging the spike of a strain of IBV for that from another strain can change the tropism of the recombinant virus.We wish to investigate the role of glycosylation of the S glycoprotein on infectivity and tropism of IBV for different cell types, both in vitro and in ovo for the development of novel vaccines.

Publications

10 25 50

publication icon
Bickerton E (2014) Cellular tropism of the infectious bronchitis virus spike glycoprotein in 8th International Symposium on Avian Corona- and Pneumoviruses and complicating pathogens 2nd Annual Meeting of Cost Action FA1207, Rauischholzhausen, Germany

publication icon
Keep S (2016) Animal Coronaviruses

publication icon
Maier HJ (2015) Preface. Coronaviruses. in Methods in molecular biology (Clifton, N.J.)

 
Description We have investigated the role of the spike glycoprotein of infectious bronchitis virus (IBV) in the ability of IBV to grow in different cell types and have developed our understanding of the spike's role in virus entry under different physiological conditions, including temperature and presence of different enzymes.

We have generated a new vaccine candidate for IBV with a modified spike that can be grown in cell culture rather than viable hen's eggs.

We have identified certain regions of the virus proteins that are modified by host cells and are important for antibody recognition. We have identified the glycans present on the spike glycoprotein of IBV.
Exploitation Route These findings may be used for rational vaccine design. In particular, the new vaccine candidate will be investigated in a recently-started Follow-on Fund grant. If the candidate is deemed successful, it will remove the need for growing such a vaccine in hen's eggs, which will be advantageous for the vaccine industry.
Sectors Agriculture, Food and Drink,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology

 
Description The findings have contributed to a successful BBSRC link grant application and have been presented at national and international conferences. We have been awarded BBSRC Follow-on Funding to continue this research into the development of vaccines against infectious bronchitis virus that can be produced in cell culture rather than eggs.
First Year Of Impact 2016
Sector Agriculture, Food and Drink,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology
Impact Types Societal

 
Description Contribution to a POSTnote on 'Reducing UK Antibiotic Use in Animals'
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description Training MSc students
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Influenced training of practitioners or researchers
Impact Delivered a new training session to MSc students teaching viral quantification assays. This lead to a greater understanding of laboratory techniques and academic research.
 
Description BBSRC responsive mode link award
Amount £1,382,000 (GBP)
Funding ID BB/P019137/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 02/2018 
End 01/2021
 
Description Further development of infectious bronchitis virus vaccines adapted for production in cell culture
Amount £262,701 (GBP)
Funding ID BB/R019576/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2018 
End 03/2020
 
Description Characterisation of IBV spike protein glycosylation using mass spectrometry 
Organisation University of Liverpool
Country United Kingdom 
Sector Academic/University 
PI Contribution Preparation of purified IBV S protein samples for mass spectrometry analysis.
Collaborator Contribution Analysis of samples by mass spectrometry to characterize glycans on the surface of the IBV S protein.
Impact Identification and structural analysis of glycans on the surface of the IBV S protein.
Start Year 2017
 
Description Partnering with National Taiwan University 
Organisation National Taiwan University
Country Taiwan, Province of China 
Sector Academic/University 
PI Contribution We have prepared and submitted an application for a BBSRC-Taiwan Partnering Award to study "Assembly of Recombinant Infectious Bronchitis Virus and Determination of Antigenic Sites that Confer Hemagglutination Activity".
Collaborator Contribution The partners conceived the project and got in contact about submitting an application for funding together.
Impact An application has been made for a BBSRC-Taiwan Partnering Award in November 2018.
Start Year 2018
 
Description Attendance of South Wiltshire Virology Group Meeting (University of Surrey) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact Scientists attended the meeting in Surrey and heard oral presentations from experts in the field as well as students and early career researchers. The event encouraged networking between colleagues and potential collaborators from other institutes and universities.
Year(s) Of Engagement Activity 2017
 
Description Avian Immunity and Genetics Meeting 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Industry/Business
Results and Impact The meeting consisted of oral and poster presentations from researchers in the field of avian immunity and genetics. I presented a poster which encouraged discussion around my project.
Year(s) Of Engagement Activity 2017
 
Description Careers fair 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Students from several schools in the region attended the careers fair where we had a stall. Several students requested information about apprenticeships and were interested in possible careers in science.
Year(s) Of Engagement Activity 2011,2012,2013,2014,2015,2016,2017
 
Description Centre of excellence for research on avian diseases (CERAD) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I was invited to present my work at the UK-China Centre of excellence for research on avian diseases (CERAD) meeting attended by researchers from UK, China and Thailand. There was lots of discussion about the research and future directions, including potential collaborations.
Year(s) Of Engagement Activity 2017
 
Description European COST Action FA1207 Early Stage Researchers Meeting (Uppsala, Sweden) 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact 15 postgraduate students from different European laboratories (all researching avian coronaviruses) attended a small focus group meeting to discuss the creation of a network for the PhD students in these research groups. This was designed to encourage inter-group collaboration and to facilitate knowledge exchange between different groups with different expertise. The visit consisted of several forums where the expertise of each group were identified and opportunities for collaboration were discussed.
Year(s) Of Engagement Activity 2015
 
Description International Day of Women and Girls in Science 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact I gave a quote as a STEM ambassador about why I think it is important to make sure women and girls have the same opportunities in STEM. This was shared on social media to celebrate International Day of Women and Girls in Science, a UN-led initiative.
Year(s) Of Engagement Activity 2019
 
Description International Women's Day video for social media 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Three team members were interviewed about their careers in science for International Women's Day. The videos were posted on Pirbright's website, Facebook and Twitter to celebrate what we love about working in science.
Year(s) Of Engagement Activity 2018
 
Description Interview for national newspaper 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact Interview with a journalist from The Telegraph about coronavirus replication and transmission.
Year(s) Of Engagement Activity 2020
 
Description Interview with Associated Press 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Interview with a journalist from Associated Press about coronavirus research at The Pirbright Institute.
Year(s) Of Engagement Activity 2020
 
Description Interview with BBC Radio Surrey 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Media (as a channel to the public)
Results and Impact Interview with journalists from BBC Radio Surrey on the breakfast show about coronavirus research at The Pirbright Institute.
Year(s) Of Engagement Activity 2020
 
Description Interview with BuzzFeed News 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact Interview with a journalist from BuzzFeed News about coronavirus research at The Pirbright Institute.
Year(s) Of Engagement Activity 2020
 
Description Invited seminar speaker 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Other audiences
Results and Impact Erica Bickerton was invited to give a seminar at Imperial College London, which sparked questions and discussion afterwards.
Year(s) Of Engagement Activity 2017
 
Description MSc student visit 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact I hosted several MSc students from University of Surrey, discussed avian research with them and demonstrated some laboratory techniques. The students gained an understanding of academic research and laboratory work.
Year(s) Of Engagement Activity 2018,2019
 
Description Microbiology Society Annual conference (UK) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Poster or oral presentation at Microbiology Society annual conference, a major international event. The participants included researchers from many different areas of science. This provides a platform to promote research to a wider audience as well as discuss the key findings with experts in my own field.
Year(s) Of Engagement Activity 2010,2011,2012,2013,2014,2015,2016,2017,2018,2019
 
Description Microbiology Society Annual conference (UK) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact At each conference I presented a poster describing a particular aspect of my project. The poster sessions were open to all participants at the conference and so the audience included researchers from many different areas of science. This provides a platform to promote my research to a wider audience as well as discuss the key findings with experts in my own field.
Year(s) Of Engagement Activity 2013,2015,2016
 
Description Microbiology Society Avian Focus Meeting (London, UK) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact I gave a 15 minute oral presentation about a part of my PhD project to an audience of around 80 people. The audience consisted of researchers from many different scientific institutions from different areas of avian research. This was a great opportunity to present my work to experts in this field and gain novel insights into the project and the possible applications of my work.
Year(s) Of Engagement Activity 2016
 
Description Microbiology Society Avian Focus Meeting (UK) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact I gave a 15 minute oral presentation to an audience of around 80 people in 2016 and 2018. The audience consisted of researchers from many different scientific institutions from different areas of avian research. This was a great opportunity to present to experts in this field and gain novel insights into the project and the possible applications of my work.
Year(s) Of Engagement Activity 2016,2018
 
Description Nidovirus symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I presented my work at the Nidovirus Symposium, which is held every three years and brings together researchers from industry and academia from many different countries. I had some interesting discussions with other researchers and built my network.
Year(s) Of Engagement Activity 2017
 
Description Pirbright Institute Student Day 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact I presented a poster describing my work to the student body and supervisors. This generated discussion and collaboration between different groups within the Institute.
Year(s) Of Engagement Activity 2015,2016
 
Description Positive Strand Viruses Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact This was a poster presentation. There were several different poster sessions at the conference allowing a large selection of people to view each poster. The audience included world leaders in my research field and important names from groups all over the world. This gave me the opportunity to discuss my work with other scientists on a one to one basis, leading to generation of new ideas for future experiments and collaborations.
Year(s) Of Engagement Activity 2010,2013,2016,2019
 
Description Positive Strand Viruses Conference (Austin, Texas, USA) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact This was a poster presentation about the work involved in my PhD project. There were several different poster sessions at the conference allowing a large selection of people to view each poster. The audience included world leaders in my research field and important names from groups all over the world. This gave me the opportunity to discuss my work with other scientists on a one to one basis, leading to generation of new ideas for future experiments and collaborations.
Year(s) Of Engagement Activity 2016
 
Description Poster presentation at Global Alliance for Research on Avian Diseases international conference (Hanoi, Vietnam) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Approximately 80 people attended the event held over 3 days. The poster encouraged discussion about the project and potential collaborations between experts in the field.
Year(s) Of Engagement Activity 2018
 
Description Poster presentation at the International Nidovirus Symposium (Kansas City, MO, USA) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Presentation of a poster to the conference delegate including experts in the fields of nidovirus and glycosylation research. The presentation sparked discussion with numerous participants and led to collaboration with one.
Year(s) Of Engagement Activity 2017
 
Description Presentation at Global Alliance for Research on Avian Diseases Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Presentation of my research at the Global Alliance for Research on Avian Diseases Conference in Vietnam to approximately 80 conference participants resulted in discussions about future research directions.
Year(s) Of Engagement Activity 2018
 
Description Presentation at RIVR meetings 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Presentation of my research at Recently Independent Virology Researchers (RIVR) meetings in 2016, 2017, 2018, 2019 and 2020. I discussed my work and future collaborations with other virology researchers working in the UK.
Year(s) Of Engagement Activity 2016,2017,2018,2019,2020
 
Description Presentation to BBSRC Executive Board 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact I presented our research to the BBSRC Executive Board when they visited The Pirbright Institute in September 2018 and discussed the impacts of our work..
Year(s) Of Engagement Activity 2018
 
Description Reverse genetics seminar (University of Surrey) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact A seminar was given based upon the reverse genetics system of infectious bronchitis virus. The audience was a group of 10 -15 MSc students from University of Surrey. The students were engaged, asked questions about the research and careers in science. We were asked to repeat the seminar for the next year's intake of students.
Year(s) Of Engagement Activity 2013,2014,2015,2016,2017,2018
 
Description School visit (Compton, West Berkshire) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact The school visit included working with small groups of a-level biology students demonstrating a technique in the curriculum. They were encouraged to ask us questions about our work and how we use the technique as well as trying it out for themselves.
Year(s) Of Engagement Activity 2013,2015
 
Description School visit (Compton, West Berkshire) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact The school visits included working with small groups of a-level biology students demonstrating a technique in the curriculum. They were encouraged to ask us questions about our work and how we use the technique as well as trying it out for themselves.
Year(s) Of Engagement Activity 2012,2013,2014,2015
 
Description Teentech 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact The annual Teentech student science event in Guildford aims to encourage teenagers to consider science subjects and science careers. Our stand had considerable interest from the schoolchildren who were very enthusiastic. The event stimulated an increased interest in science and research.
Year(s) Of Engagement Activity 2016,2019
 
Description organised workshops at Microbiology Society annual conferences 2014 - 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I co-organised virology division workshops on viral evolution and diversity and RNA viruses with colleagues from the virology division. The workshops were held at the annual Microbiology Society conferences in spring each year. Organisation involved selecting abstracts for oral or poster presentation, ordering the presentations and chairing the sessions.
Year(s) Of Engagement Activity 2015,2016,2017,2018