Recognition and control of virus infections

Lead Research Organisation: The Pirbright Institute
Department Name: UNLISTED

Abstract

The activities contributing to this objective examine the underlying basis behind the induction and effector phases of immune responses from pathogen recognition through to virus elimination and the formation of memory responses. These complex and interdependent events can now be explored at a resolution that was previously impossible, owing to recent advances in high throughput screening methods, recombinant technologies and imaging techniques. The immune responses of the three key species studied at Pirbright (cattle, pigs and chickens) will all be examined at the molecular and cellular level during natural viral infection and vaccination studies. This will include the use of inbred and transgenic chickens, as well as our inbred pigs and major histocompatibility complex (MHC) homozygous cattle. As these species are the natural viral hosts, our studies have the potential to immediately inform strategies that improve the duration and broaden the efficacy of current vaccines as well as underpin the rational design of the next generation of vaccines.
Viruses have evolved an array of strategies to favourably influence host responses after infection. After cell entry, viruses influence innate and adaptive responses by interfering with multiple intracellular and intercellular pathways, for example proteins of many viruses inhibit specific immune recognition mechanisms. Our work in the natural hosts using well characterised and recombinant viruses will establish how different viruses can alter both immune activation and regulation pathways.
T and B cells are the major effector cells of the adaptive immune system being able to distinguish between self and pathogens, mount a specific response and reside after the infection to provide immunological memory. Vaccines remain by far the best method to control viral diseases. Consequently T and B cell receptor diversity and their protective roles are a major component of research activities at Pirbright during infection and vaccination.
Decades of existing work with influenza and human immunodeficiency viruses has demonstrated that even antigenically variable viruses possess conserved, broadly neutralising antibody eliciting epitopes, but that they are weakly immunogenic. Many of the viruses studied at Pirbright possess regions of high antigenic variability and dominant epitopes that don’t induce broadly neutralising antibody responses. However, it is now clear that sub dominant epitopes and cross-reactive epitopes exist with enormous potential to inform vaccine antigen engineering to boost immunogenicity. Among potential candidate vaccine antigens for each pathogen only a minority induce protective immunity. Therefore, the identification of protective B and T cell antigens and epitopes is crucial for vaccine development and for evaluation of their immunogenicity and efficacy. We will identify and characterise T and B cell antigens and epitopes derived from a range of veterinary species and their viral pathogens, focussing on identification of conserved antigenic targets that may provide broad cross-protection.
The immunogenicity and efficacy of vaccines is both quantitatively and qualitatively dependent upon the delivery system used. We will evaluate delivery platforms including virus-like particles (VLP), empty capsids, nanocarriers, viral vectors or live attenuated viruses to improve the immunogenicity and efficacy of vaccines. The combination of vaccine vector technology and targeted delivery of antigen-antibody fusions will facilitate delivery of protective antigens and induce robust humoral and cell-mediated immunity against target viruses.

Organisations

Publications

10 25 50
 
Description Key findings:

The current BBSRC-funded Institute Strategic Project "Recognition and control of virus infections" has been underway for just under 12 months. Here we have focused on key findings from research carried out at Pirbright aiming to improve the control of three economically important diseases of pigs - porcine respiratory and reproductive syndrome virus, African swine fever virus, and swine influenza A virus - and how this work has underpinned new areas of porcine research at Pirbright.

Cross-protective antibodies to porcine respiratory and reproductive syndrome virus (PRRSV): We have demonstrated that a 'genetic programming' approach is highly effective at transforming porcine B cells into proliferative antibody-secreting cells. This breakthrough greatly facilitates studies into protective humoral response to PRSV in pigs. An experimental heterologous PRRSV challenge study was conducted in pigs to induce high titres of neutralising antibodies, and we are currently cloning memory B cells from these animals, using the "genetic programming" approach in order to produce a panel of neutralising monoclonal antibodies that may be used in downstream studies to elucidate novel epitopes.
African swine fever virus (ASFV) inhibitors of type I interferon (IFN): In previous work we showed that deletion or interruption of multiple genes from the gene clusters MGF360 and MGF505 in the virulent ASFV isolate Benin 97/1 did not reduce virus replication in macrophages but did result in increased transcript levels for IFN-beta suggesting these genes have a role in suppressing the host anti-viral IFN response to ASFV. In follow on work since April 2017 we have screened for other ASFV genes encoding proteins that may also inhibit IFN induction and could therefore be additional candidates for deletion in order to develop a safe live-attenuated vaccine strain of ASFV. We constructed 60 plasmids each expressing an individual ASFV gene, expressed them in cultured cells, and tested the proteins for their ability to inhibit expression of IFN pathways. 15 proteins were identified that inhibited IFN induction. Future work will define the mode of action of these proteins and their role in host/virus interactions.
Identifying influenza-derived peptide sequences capable of stimulating T-cells: A major roadblock in studies of pig immunology is our inability to analyze antigen-specific CD8+ T-cell responses, which are particularly critical to immunity. This knowledge gap was addressed using in-bred pigs ("Babraham pigs") that have a high degree of genetic identity between individuals, including the MHC (Swine Leukocyte Antigen (SLA)) locus. We developed a toolset including long-term in vitro pig T-cell culture, and used them to clone and identify novel immunodominant T-cell epitopes in pigs challenged with influenza A virus.
Following on from the identification of immunodominant influenza-derived T-cell epitopes, we generated structures of the two SLA class I molecules found in the Babraham pigs that present the immunodominant epitopes. This allowed identification of the primary anchor points for the influenza epitopes in the SLA binding groove, and established SLA binding motifs that were used to successfully predict other influenza-derived peptide sequences capable of stimulating T-cells. Peptide-SLA tetramers were constructed and used to track influenza-specific T-cells ex vivo in blood, the lungs and draining lymph nodes. Aerosol immunization with attenuated single cycle influenza viruses (S-FLU) was found to induce large numbers of CD8+ T-cells specific for conserved NP peptides in the respiratory tract.
Collectively, these data substantially increase the utility of pigs as an effective model for studying protective local cellular immunity. These tools and knowledge will be applied to research into other pig viral pathogens such as ASFV in the near future.

Nipah virus vaccine grant success: ISP supported porcine vaccinology and immunology research was a significant factor in Pirbright leading the successful bid by a large consortium to the Innovate UK Small Business Research Initiative: Vaccines for Global Epidemics competition. "A Nipah virus vaccine to eliminate porcine reservoirs and safeguard human health" is a £2 348 798, three year, multi-partner project that aims to develop an inexpensive, safe and effective vaccine to protect pigs against Nipah virus (NiV). The project involves a number of new collaborations with partners in Australia, Malaysia and India, opening up new avenues for adding further value to the ISP-supported work at Pirbright.
Exploitation Route Partnerships with veterinary vaccine manufacturers will be the key mechanism by which the findings described above will be taken forward.
Sectors Agriculture, Food and Drink

 
Description Chair of BSI Comparative Veterinary Immunology Group
Geographic Reach National 
Policy Influence Type Influenced training of practitioners or researchers
 
Description Contribution to Annual Report of the Chief Medical Officer UK (2017), Genomics Chapter 9.
Geographic Reach Europe 
Policy Influence Type Gave evidence to a government review
 
Description Human Infection Challenge Models for Vaccination
Geographic Reach Europe 
Policy Influence Type Influenced training of practitioners or researchers
 
Description Lumpy Skin Disease Control Strategy for Great Britain
Geographic Reach National 
Policy Influence Type Participation in a advisory committee
 
Description Organised seminar series on Sequencing, Virus Evolution and Molecular Biology at Pirbright (with Luca Ferretti and Graham Freimanis)
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Influenced training of practitioners or researchers
 
Description Training MSc students
Geographic Reach Local/Municipal/Regional 
Policy Influence Type Influenced training of practitioners or researchers
Impact Delivered a new training session to MSc students teaching viral quantification assays. This lead to a greater understanding of laboratory techniques and academic research.
 
Description "Vaccines for Global Epidemics: A Nipah virus vaccine to eliminate porcine reservoirs and safeguard human health"
Amount £2,348,798 (GBP)
Organisation Innovate UK 
Sector Public
Country United Kingdom
Start 09/2017 
End 09/2020
 
Description Active and passive Immunity induced by aerosols
Amount £100,000 (GBP)
Funding ID BB/R506448/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2017 
End 08/2021
 
Description BBSRC Industrial CASE (iCASE) Studentship: Enhancing protective efficacy of avian influenza vaccines through targeted delivery of protective antigens to chicken immune cells.
Amount £98,212 (GBP)
Funding ID BB/R50595X/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2017 
End 10/2021
 
Description BBSRC International Partnering Awards
Amount £49,500 (GBP)
Funding ID BB/P025803/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 08/2017 
End 07/2021
 
Description BBSRC Newton Fund Swine and Poultry Research Initiative
Amount £865,623 (GBP)
Funding ID BB/R01275X/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 04/2018 
End 06/2021
 
Description BBSRC Responsive Mode IPA
Amount £302,086 (GBP)
Funding ID BB/R002606/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2018 
End 06/2019
 
Description BBSRC Responsive Mode funding
Amount £595,574 (GBP)
Funding ID BB/R008833/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 05/2018 
End 04/2021
 
Description BBSRC iCASE) Studentship: Enhancing protective efficacy of avian influenza vaccines through targeted delivery of protective antigens to chicken immune cells.
Amount £98,212 (GBP)
Funding ID BB/R50595X/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 10/2018 
End 10/2022
 
Description Biotechnology and Biological Sciences Research Council (BBSRC) and University of Oxford joint Doctoral Training Project
Amount £100,000 (GBP)
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2018 
End 08/2022
 
Description British Egg Marketing Board PhD studentship
Amount £76,000 (GBP)
Organisation British Egg Marketing Board 
Sector Private
Country United Kingdom
Start 10/2018 
End 09/2022
 
Description Japan Partnering Award
Amount £49,500 (GBP)
Funding ID BB/P025803/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 09/2017 
End 08/2021
 
Description LVIF
Amount $1,500,000 (CAD)
Organisation Bill and Melinda Gates Foundation 
Sector Charity/Non Profit
Country United States
Start 04/2018 
End 09/2020
 
Description NC3Rs Research Project Grant
Amount £436,339 (GBP)
Funding ID NC/R001138/1 
Organisation National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) 
Sector Private
Country United Kingdom
Start 12/2017 
End 11/2019
 
Description Newton Fund: UK-China-Philippines-Thailand Swine and Poultry Research Initiative. Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry.
Amount £497,995 (GBP)
Funding ID BB/R012679/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 01/2018 
End 12/2020
 
Description Newton Institutional Links grant
Amount £269,123 (GBP)
Funding ID 261727271 
Organisation Newton Fund 
Sector Public
Country United Kingdom
Start 09/2017 
End 08/2019
 
Description Pathfinder BB/R012431/1 Grant holder: Dr Mark Fife. Grant title: chIFITM knockdown/knockout technology as a platform technology for increased vaccine yields in SPF Eggs.
Amount £12,500 (GBP)
Funding ID BB/R012431/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom
Start 11/2017 
End 02/2018
 
Description Pirbright Institute Agri-Food Technology Seed Fund: Production of prototype lateral flow device (LFD) for detection/ diagnosis of H7 avian influenza virus infection in birds and humans. Value £30,000.
Amount £30,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 11/2017 
End 11/2018
 
Description Pirbright Institute PhD studentship
Amount £100,000 (GBP)
Organisation The Pirbright Institute 
Sector Academic/University
Country United Kingdom
Start 09/2018 
End 08/2022
 
Title An ex vivo IBDV infection model using a chicken primary B cell culture system 
Description Recently, it has become possible to culture chicken primary B cells in vitro in the presence of a soluble construct of chicken CD40L that was made at The Pirbright Institute. We have demonstrated that these chicken primary B cells can be infected with infectious bursal disease virus (IBDV). This system will be validated and expanded to other B cell tropic viruses in order to replace the use of infected chickens in research, in an NC3Rs funded grant. 
Type Of Material Model of mechanisms or symptoms - in vitro 
Year Produced 2017 
Provided To Others? Yes  
Impact Using this system, we have determined that chicken primary B cells respond to IBDV infection by inducing Type I IFN responses. However, the induction is more pronounced in cells infected with an attenuated strain compared to a very virulent strain. This is consistent with the very virulent strain down-regulating antiviral responses to a greater extent than other strains which may, in part, explain its enhanced virulence. We also observed a reduction in the expression of key genes involved in B cell proliferation and activation following IBDV infection which was only possible by using the primary B cell culture system. 
URL https://www.ncbi.nlm.nih.gov/pubmed/?term=broadbent+dulwich
 
Title De 
Description // 
Type Of Material Model of mechanisms or symptoms - human 
Year Produced 2017 
Provided To Others? No  
Impact // 
 
Title Development of tetramers to study immune responses in pigs 
Description The pig is a natural and important host of influenza viruses and is physiologically more comparable to humans than other animal models in terms of size, respiratory tract biology and volume. It is also an important vector in the birds to human infection cycle. A major drawback of the current pig model is the inability to analyze antigen-specific CD8+ T-cell responses, which are critical to respiratory immunity. We addressed this knowledge gap using an established in-bred pig model with a high degree of genetic identity between individuals, including the MHC (Swine Leukocyte Antigen (SLA)) locus. We developed a toolset that included long-term in vitro pig T-cell culture and cloning and identification of novel immunodominant influenza-derived T-cell epitopes. We also generated structures of the two SLA class I molecules found in these animals presenting the immunodominant epitopes. These structures allowed definition of the primary anchor points for epitopes in the SLA binding groove and established SLA binding motifs that were used to successfully predict other influenza-derived peptide sequences capable of stimulating T-cells. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? No  
Impact Peptide-SLA tetramers were constructed and used to track influenza-specific T-cells ex vivo in blood, the lungs and draining lymph nodes. Aerosol immunization with attenuated single cycle influenza viruses (S-FLU) induced large numbers of CD8+ T-cells specific for conserved NP peptides in the respiratory tract. Collectively, these data substantially increase the utility of pigs as an effective model for studying protective local cellular immunity against respiratory pathogens. 
 
Title Genome enrichement with long read sequencing 
Description We have developed the Nimblegen-Roche SeqCapEZ probes based pull down method to work with genomic DNA fragments up to 6kb in length with PacBio sequencing. This is being used for de novo assembly of variable regions 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact We are now involved with industry to refine this protocol further with less input DNA and high multiplex capability. 
 
Title IBDV Reverse Genetics System 
Description We have developed a reverse genetics system for IBDV. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact We have used the reverse genetics system to generate recombinant IBDVs and chimeric recombinant IBDVs that contain genes from classical and very virulent strains of IBDV in the background of a cell culture adapted attenuated strain. This will enable us to study the effect of individual virus genes on pathogenicity. 
 
Title Optimisation of chicken kidney cell culture preparation 
Description We have optimised the method of extracting kidneys and preparing chicken kidney (CK) cell cultures from chickens. CK cells are used for in vitro assays to study avian viruses, in particular infectious bronchitis virus. 
Type Of Material Cell line 
Year Produced 2017 
Provided To Others? No  
Impact This has increased the yield of viable cells and the quality of the cell cultures. We can now obtain the number of cells we require using fewer chicken kidneys, therefore requiring fewer chickens to be culled. This is an important development for the 3Rs. We hope to publish this method for others' information. 
 
Title Stable chicken cell lines overexpressing chicken IFITM1,2 and 3 
Description In collaboration with Mark Fife, we have produced chicken stable cell lines overexpressing chicken IFITM1,2, 3 and a mutant chicken IFITM3 lacking palmitoylation sites. 
Type Of Material Technology assay or reagent 
Year Produced 2017 
Provided To Others? No  
Impact These cell lines will be used to evaluate the effect of the IFITM proteins on the replication of different viruses, including IBDV. 
 
Title The application of NHEJ-CRISPR/Cas9 and Cre-Lox system in the generation of bivalent duck enteritis virus vaccine against avian influenza virus 
Description We developed integrated non-homologous end-joining (NHEJ)- clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 system and Cre-Lox system for generating recombinant Duck enteritis virus (DEV)as vectored vaccines against the avian influenza virus (AIV). This is an efficient and rapid method for generation of a recombinant DEV-AIV vaccine. The vaccine provide protection of ducks from AIV and DEV and enable differentiation between infected and vaccinated animals (DIVA) approach. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? Yes  
Impact Increased research collaboration and research funding from Industry 
URL http://www.mdpi.com/1999-4915/10/2/81
 
Title method for identification of lung Tissue resident memory cells in pigs 
Description Recent overwhelming evidence indicates the importance of local tissue-resident memory T cells (TRM) in protective immunity. Most work on TRM has been performed in mice and the TRM defined as inaccessible to intravenously administered anti-T cell antibody. However there are very few data on TRM in large animals. For the first time we have defined TRM in the pig influenza model. 
Type Of Material Technology assay or reagent 
Year Produced 2018 
Provided To Others? No  
Impact The identification of TRM and their robust protective capacity in site-specific infection has provided a new paradigm by which to assess T cell-mediated responses and an important new target for vaccine design. Since swine are an economically important species, are used as a large animal model for human infection and play a key role in the emergence of novel and potentially zoonotic influenza viruses, the identification of TRM in pigs will allow us to study their role in immunity to swine influenza.and how best to induce them by immunisation. 
 
Title E-MTAB-5947- Transcription profiling by array of differential gene expression in chicken primary B cells infected ex vivo with attenuated and very virulent strains of infectious bursal disease virus (IBDV) 
Description Microarray data submitted to ArrayExpress from chicken primary B cells infected with a very virulent and attenuated strain of IBDV. 
Type Of Material Database/Collection of data 
Year Produced 2017 
Provided To Others? Yes  
Impact This is the first ever microarray data of chicken primary B cells infected with IBDV and will be of use to other researchers in the area of B cell biology or IBDV virology 
URL https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5947/
 
Title Viral assembly pipeline 
Description The Integrative Biology and Bioinformatics group has developed a data analysis pipeline to obtain sensitive and robust viral assemblies out of high-throughput sequencing data. In spite of the relatively short genomic length of most viruses, assembling of viral sequences can be challenging due to several reasons, such as: low amount of material in the sample, which might require amplification, introducing biases, and/or generates low-quality sequences; uneven coverage due to low-quality material or the genomic material of the virus being RNA; the viral nucleic acid being almost lost in the background of nucleic acid of the host. Our pipeline overcomes most of those problems, and is even able to detect different viral strains being present together in the same sample. It can also be used on metagenomic environmental samples. 
Type Of Material Data analysis technique 
Year Produced 2017 
Provided To Others? No  
Impact The pipeline is being deployed as the tool of choice for the nascent sequencing facility at Pirbright. It has been used by several groups at Pirbright, and the FMDV World Reference Lab, in order to assemble very different kinds of viruses. 
 
Title Viral variant calling pipeline 
Description The Integrative Biology and Bioinformatics group has developed a data analysis pipeline to obtain sensitive and robust variant calling for viral sequences out of high-throughput sequencing data. In spite of the relatively short genomic length of most viruses, calling variant for viral sequences can be challenging due to several reasons, such as: low amount of material in the sample, which might require amplification, introducing biases, and/or generates low-quality sequences; very high genome coverage, which slows down most existing variant callers; the presence of a potentially very high number of different haplotypes for RNA viruses, which is an unusual scenario in variant calling. Our fast and sensitive Bayesian pipeline overcomes most of those problems. 
Type Of Material Data analysis technique 
Year Produced 2017 
Provided To Others? No  
Impact The pipeline is being deployed as the tool of choice for the nascent sequencing facility at Pirbright. It has been used by several groups at Pirbright, and the FMDV World Reference Lab, in order to assemble very different kinds of viruses. 
 
Description Aerosol delivery of vaccines and therapeutics 
Organisation Aerogen
Country Ireland 
Sector Private 
PI Contribution Developed the pig influenza model which is a natural host pathogen system
Collaborator Contribution Provided expertise and equipment for aerosol delivery
Impact Successfully delivered vaccines by aerosol
Start Year 2015
 
Description Anopheles reannotation 
Organisation Centre for Genomic Regulation (CRG)
Country Spain 
Sector Academic/University 
PI Contribution Provided RNA-sequencing data (produced by Jaroslaw Krzywinski and analysed by us)
Collaborator Contribution Using the data to produce a reannotation of Anopheles Gambiae
Impact No outputs yet
Start Year 2017
 
Description Avian influenza H7N9 virus evolution: defining the impact of internal genes on virus infection in avian and mammalian species 
Organisation Francis Crick Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution (1) Exchange of research methods and reagents
Collaborator Contribution (1) Exchange of research methods and reagents. (2) Provision of training to PhD students and postdoctoral Scientist (3) Critical review of experimental data and manuscripts.
Impact (1) Joint funding: BBSRC Newton Fund: UK-China-Philippines-Thailand Swine and Poultry Research Initiative (2018-2021). Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry. Value: £497,995.93. UK Co-Is: Prof John McCauley (Crick Institute), Dr Richard Reeve (University of Glasgow). Collaborators from China: Dr Yipeng Sun, Prof Jinhua Liu and Dr Juan Pu (China Agricultural University), Prof Yongqing Li (Beijing Academy of Agriculture and forestry Sciences), Dr Wenfei Zhu (National Institute for Viral Disease Control and Prevention, China CDC).
Start Year 2007
 
Description Bridget Penman 
Organisation University of Warwick
Department School of Life Sciences
Country United Kingdom 
Sector Academic/University 
PI Contribution We are providing genetic data and diversity measures of MHC and associated receptors to inform the modelling efforts to elucidate which selection pressures are the main drivers for the types of diversity we see in extant species.
Collaborator Contribution They are experts in mathmatical modelling and will provide data to help explain genetic diversity in cattle.
Impact Not yet
Start Year 2017
 
Description Cost effectiveness of interventions for the control of avian Influenza virus infections in poultry in Pakistan. 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution Capacity building, provision of training, sharing research samples and methods
Collaborator Contribution Provision of Field data on prevalence of influenza viruses in poultry production systems .
Impact 1. Mapping of the broiler production industry and trade patterns in Pakistan presented by Hassaan bin Aslam (PhD student)
Start Year 2016
 
Description Cost effectiveness of interventions for the control of avian Influenza virus infections in poultry in Pakistan. 
Organisation University of Veterinary & Animal Sciences
Department Department of Microbiology
PI Contribution Capacity building, provision of training, sharing research samples and methods
Collaborator Contribution Provision of Field data on prevalence of influenza viruses in poultry production systems .
Impact 1. Mapping of the broiler production industry and trade patterns in Pakistan presented by Hassaan bin Aslam (PhD student)
Start Year 2016
 
Description Development of novel diagnostic tools and vaccines 
Organisation Francis Crick Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution The outcomes of research led to incite new collaborations with following partners in UK, Vietnam and Pakistan. Together with partners we apply for the collaborative research grant to BBSRC entitled "Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines". That led to further improvement avian influenza virus vaccines and diagnostics and a number of research publication.
Collaborator Contribution The partners are evaluating the potency and efficacy of vaccines in countries where the disease is enzootic in poultry.
Impact 1. Peacock TP, Benton DJ, Sadeyen J-R, Chang P, Sealy JE.,d, Bryant JE, Martin SR., Shelton H, McCauley JW, Barclay WS, Iqbal M (2017) Variability in H9N2 haemagglutinin receptor binding preference and pH of fusion. Emerging Microbes & Infections 6, e11 2. Peacock T, Reddy K, James J, Adamiak B, Barclay W, Shelton H, Iqbal M (2016). Antigenic mapping of an H9N2 avian influenza virus reveals two discrete antigenic sites and a novel mechanism of immune escape. Scientific Reports 5: 18745. doi: 10.1038/srep18745. 3. Thuy DM, Peacock TP., Bich VTN, . Iqbal M, Juliet E. Bryant (2016). Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014. Infection, Genetics and Evolution 44: 530-540
Start Year 2014
 
Description Development of novel diagnostic tools and vaccines 
Organisation Imperial College London
Department School of Public Health
Country United Kingdom 
Sector Academic/University 
PI Contribution The outcomes of research led to incite new collaborations with following partners in UK, Vietnam and Pakistan. Together with partners we apply for the collaborative research grant to BBSRC entitled "Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines". That led to further improvement avian influenza virus vaccines and diagnostics and a number of research publication.
Collaborator Contribution The partners are evaluating the potency and efficacy of vaccines in countries where the disease is enzootic in poultry.
Impact 1. Peacock TP, Benton DJ, Sadeyen J-R, Chang P, Sealy JE.,d, Bryant JE, Martin SR., Shelton H, McCauley JW, Barclay WS, Iqbal M (2017) Variability in H9N2 haemagglutinin receptor binding preference and pH of fusion. Emerging Microbes & Infections 6, e11 2. Peacock T, Reddy K, James J, Adamiak B, Barclay W, Shelton H, Iqbal M (2016). Antigenic mapping of an H9N2 avian influenza virus reveals two discrete antigenic sites and a novel mechanism of immune escape. Scientific Reports 5: 18745. doi: 10.1038/srep18745. 3. Thuy DM, Peacock TP., Bich VTN, . Iqbal M, Juliet E. Bryant (2016). Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014. Infection, Genetics and Evolution 44: 530-540
Start Year 2014
 
Description Development of novel diagnostic tools and vaccines 
Organisation National Agricultural Research Centre
Country Pakistan 
Sector Public 
PI Contribution The outcomes of research led to incite new collaborations with following partners in UK, Vietnam and Pakistan. Together with partners we apply for the collaborative research grant to BBSRC entitled "Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines". That led to further improvement avian influenza virus vaccines and diagnostics and a number of research publication.
Collaborator Contribution The partners are evaluating the potency and efficacy of vaccines in countries where the disease is enzootic in poultry.
Impact 1. Peacock TP, Benton DJ, Sadeyen J-R, Chang P, Sealy JE.,d, Bryant JE, Martin SR., Shelton H, McCauley JW, Barclay WS, Iqbal M (2017) Variability in H9N2 haemagglutinin receptor binding preference and pH of fusion. Emerging Microbes & Infections 6, e11 2. Peacock T, Reddy K, James J, Adamiak B, Barclay W, Shelton H, Iqbal M (2016). Antigenic mapping of an H9N2 avian influenza virus reveals two discrete antigenic sites and a novel mechanism of immune escape. Scientific Reports 5: 18745. doi: 10.1038/srep18745. 3. Thuy DM, Peacock TP., Bich VTN, . Iqbal M, Juliet E. Bryant (2016). Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014. Infection, Genetics and Evolution 44: 530-540
Start Year 2014
 
Description Development of novel diagnostic tools and vaccines 
Organisation University of Oxford
Department Oxford University Clinical Research Unit Vietnam (OUCRU)
Country Viet Nam 
Sector Academic/University 
PI Contribution The outcomes of research led to incite new collaborations with following partners in UK, Vietnam and Pakistan. Together with partners we apply for the collaborative research grant to BBSRC entitled "Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines". That led to further improvement avian influenza virus vaccines and diagnostics and a number of research publication.
Collaborator Contribution The partners are evaluating the potency and efficacy of vaccines in countries where the disease is enzootic in poultry.
Impact 1. Peacock TP, Benton DJ, Sadeyen J-R, Chang P, Sealy JE.,d, Bryant JE, Martin SR., Shelton H, McCauley JW, Barclay WS, Iqbal M (2017) Variability in H9N2 haemagglutinin receptor binding preference and pH of fusion. Emerging Microbes & Infections 6, e11 2. Peacock T, Reddy K, James J, Adamiak B, Barclay W, Shelton H, Iqbal M (2016). Antigenic mapping of an H9N2 avian influenza virus reveals two discrete antigenic sites and a novel mechanism of immune escape. Scientific Reports 5: 18745. doi: 10.1038/srep18745. 3. Thuy DM, Peacock TP., Bich VTN, . Iqbal M, Juliet E. Bryant (2016). Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014. Infection, Genetics and Evolution 44: 530-540
Start Year 2014
 
Description Development of novel diagnostic tools and vaccines 
Organisation University of Veterinary & Animal Sciences
Country Pakistan 
Sector Academic/University 
PI Contribution The outcomes of research led to incite new collaborations with following partners in UK, Vietnam and Pakistan. Together with partners we apply for the collaborative research grant to BBSRC entitled "Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines". That led to further improvement avian influenza virus vaccines and diagnostics and a number of research publication.
Collaborator Contribution The partners are evaluating the potency and efficacy of vaccines in countries where the disease is enzootic in poultry.
Impact 1. Peacock TP, Benton DJ, Sadeyen J-R, Chang P, Sealy JE.,d, Bryant JE, Martin SR., Shelton H, McCauley JW, Barclay WS, Iqbal M (2017) Variability in H9N2 haemagglutinin receptor binding preference and pH of fusion. Emerging Microbes & Infections 6, e11 2. Peacock T, Reddy K, James J, Adamiak B, Barclay W, Shelton H, Iqbal M (2016). Antigenic mapping of an H9N2 avian influenza virus reveals two discrete antigenic sites and a novel mechanism of immune escape. Scientific Reports 5: 18745. doi: 10.1038/srep18745. 3. Thuy DM, Peacock TP., Bich VTN, . Iqbal M, Juliet E. Bryant (2016). Prevalence and diversity of H9N2 avian influenza in chickens of Northern Vietnam, 2014. Infection, Genetics and Evolution 44: 530-540
Start Year 2014
 
Description Development of tetramers in the Babraham pig model 
Organisation Cardiff University
Country United Kingdom 
Sector Academic/University 
PI Contribution Developed the aerosol delivery method of vaccine and provided material (spleen, BAL and blood) from inbred animals (Babraham pigs) following immunisation with the candidate universal vaccine, S-FLU.
Collaborator Contribution Developed porcine influenza specific class I tetramers and provided the epitope map of Babrahams S-Flu responses to NP, M1, M2, PB1 and PB2 that could be presented by SLA-1 or SLA-2 Grew pig T cell clones for the first time in 30 years.
Impact Multidisciplinary - immunology, virology, protein chemistry. Paper not yet published
Start Year 2014
 
Description EBI Culicoides genome project 
Organisation EMBL European Bioinformatics Institute (EMBL - EBI)
Country United Kingdom 
Sector Academic/University 
PI Contribution Performed experiments and provided material for Sequencing and genome build of the Culicoides genome project
Collaborator Contribution Sequencing and genome build for Culicoides genome project.
Impact Publication and public release of genome data for Culicoides genome project
Start Year 2013
 
Description Efficacy of a candidate universal influenza vaccine, S-FLU. 
Organisation University of Oxford
Department Radcliffe Department of Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution Tested the immunogenicity and efficacy of a candidate universal influenza vaccine, S-FLU, in pigs. For the first time we demonstrated that S-FLU when administered by aerosol can reduce viral load in nasal swabs and lung in pigs after influenza virus challenge. We have shown that the most efficient way to administer this vaccine is by aerosol.
Collaborator Contribution Professor Alain Townsend has developed the S-FLU vaccine.
Impact The most efficient way to induce immune response in the lung is after aerosol delivery of LAIV vaccines in pigs. The most efficient way to induce cross-protective immunity is by aerosol delivery of S-FLU to the lungs of pigs
Start Year 2014
 
Description Enhancing protective efficacy of avian influenza vaccines through targeted delivery of protective antigens to chicken immune cells 
Organisation University of Oxford
Department Department of Zoology
Country United Kingdom 
Sector Academic/University 
PI Contribution Enhancing protective efficacy of avian influenza vaccines through targeted delivery of protective antigens to chicken immune cells ( PhD student training.
Collaborator Contribution Training of next generation of Scientists (PhD student training).
Impact Training of next generation of Scientists (PhD student training).
Start Year 2017
 
Description Evaluation of artificial cell membranes for delivery of PRRSV antigens 
Organisation ACM Biolabs
PI Contribution Ability to evaluate the immunogenicity of artificial cell membranes (ACMs) for delivery of PRRSV antigens
Collaborator Contribution To use their patented platform technology to produce a panel of ACMs embedded with different PRRSV envelope proteins
Impact Not yet
Start Year 2017
 
Description Evaluation of porcine cytomegalovirus as a viral vaccine vector 
Organisation University of Plymouth
Country United Kingdom 
Sector Academic/University 
PI Contribution PRRSV model and vaccine candidate antigens. Expertise in porcine T cell immunology
Collaborator Contribution Expertise in exploiting herpesviruses as vaccine vectors
Impact not yet
Start Year 2015
 
Description Evaluation of the efficacy of antiviral nanoparticles (AVNPs) against PRRSV 
Organisation University of Hertfordshire
Country United Kingdom 
Sector Academic/University 
PI Contribution Expertise in PRRSV and in vitro model to assess efficacy of AVNPs
Collaborator Contribution Expertise in materials science
Impact Not yet
Start Year 2016
 
Description Evaluation of virus-imprinted polymers as synthetic neutralisng antibodies 
Organisation University of Central Lancashire
Country United Kingdom 
Sector Academic/University 
PI Contribution Model viruses and systems to evaluate the ability of molecularly-imprinted polymers to neutralise virus infectivity
Collaborator Contribution Expertise in molecularly-imprinted polymers
Impact Wellcome Trust Seed Award
Start Year 2015
 
Description Genetic mapping of vector competence in Culicoides sonorensis. 
Organisation EMBL European Bioinformatics Institute (EMBL - EBI)
Country United Kingdom 
Sector Academic/University 
PI Contribution The primary deliverable of this project will be the elucidation of the genetic basis of vector competence of C. sonorensis for bluetongue virus (BTV) and African horse sickness virus (AHSV). It will also generate the first accurately annotated genome of a Culicoides species worldwide.
Start Year 2012
 
Description Horizon Discovery Ltd Cambridge Research Park, United Kingdom 
Organisation Horizon Discovery Group plc
PI Contribution Verify IFITM knock-out cell by qPCR and immuno-blotting. Verification of further knock-out cell lines by DNA sequencing, immuno-blotting and qPCR Cell infection with Avian Influenza Viruses Assess the viral titre of cells infected with Influenza Viruses in K/O and wildtype cells. Quantitative analysis of viral infection in IFITM K/O cells Demonstrate increased AIV viral titres at lab scale and estimation of what this may equate to with respect to vaccine dose yields. Analysis of IFITM K/O cell lines permissivity to a range of additional animal viruses (Relevant to the LVIF). Milestone 7: Demonstrate permissivity increased and viral titres for a range of LVIF relevant viruses in the edited cell lines.
Collaborator Contribution Develop a CRISPR/cas9 gene editing system to specifically target the chIFITM locus in avian cell culture
Impact Grant funding
Start Year 2017
 
Description ILRI Research Collaboration (Kenya, March 2017) 
Organisation International Livestock Research Institute (ILRI)
Country Kenya 
Sector Charity/Non Profit 
PI Contribution Exchange of ideas and samples for indigenous breeds of livestock
Collaborator Contribution Contributed samples for analysis.
Impact Analysis of indigenous breed genomic sequences for Immune function genes.
Start Year 2017
 
Description Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design. 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution We have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Collaborator Contribution My collaborators have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Impact Submission of proposal entitled "Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design" to the "One Health Approaches to Accelerate Vaccine Development" call that forms part of the UK government's commitment to Official Development Assistance (ODA) in October 2017.
Start Year 2017
 
Description Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design. 
Organisation University of Kent
Country United Kingdom 
Sector Academic/University 
PI Contribution We have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Collaborator Contribution My collaborators have performed experiments to generate preliminary data for a collaborative grant application, contributed to the writing of the grant proposal and responded to reviewers comments.
Impact Submission of proposal entitled "Identification of conserved B-cell epitopes of highly pathogenic coronaviruses for broadspectrum immunotherapy and vaccine design" to the "One Health Approaches to Accelerate Vaccine Development" call that forms part of the UK government's commitment to Official Development Assistance (ODA) in October 2017.
Start Year 2017
 
Description Identification of genetic markers in the haemagglutinin glycoprotein critical for antigenic activity of H9N2 avian influenza viruses. 
Organisation Francis Crick Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution d provision of reagents and virus samples.
Collaborator Contribution Technical training to PhD students and use of research facilities
Impact (1) Talk: Avian influenza virus evolution: immune escape, increase in zoonotic potential and fitness in poultry. Presented at "Prevention and Control Techniques for Infectious Diseases in Livestock and Poultry", 27th 29th August 2017, Beijing, China. (2) Impact of avian influenza virus evolution on antigenicity and zoonotic infection potential. Presented at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan on 23rd August 2017. (3). Molecular determinants of H9N2 avian influenza virus influencing antigenicity and receptor binding. Presented at CERAD (Centre of Excellence for Research on Avian Diseases), 3rd Symposium Recent Advances in Avian Disease Research. 31st August - 1st September 2017 at the Harbour Hotel, Alexandra Terrace, Guildford, Surrey, UK. (4) Overview of ongoing research on Poultry diseases in United Kingdom. Presented as at International poultry Stakeholders work on poultry biosecurity in Sri Lanka from 26th February to 3rd March 2017. (5). Evolutionary fitness of avian influenza viruses and development of improved vaccines and diagnostics. Presented as invited seminar speaker at RVC (Hawkshead Campus), UK. 19th October 2016.
Start Year 2012
 
Description Identification of genetic markers in the haemagglutinin glycoprotein critical for antigenic activity of H9N2 avian influenza viruses. 
Organisation Imperial College London
Department Section of Virology
Country United Kingdom 
Sector Academic/University 
PI Contribution d provision of reagents and virus samples.
Collaborator Contribution Technical training to PhD students and use of research facilities
Impact (1) Talk: Avian influenza virus evolution: immune escape, increase in zoonotic potential and fitness in poultry. Presented at "Prevention and Control Techniques for Infectious Diseases in Livestock and Poultry", 27th 29th August 2017, Beijing, China. (2) Impact of avian influenza virus evolution on antigenicity and zoonotic infection potential. Presented at University of Veterinary and Animal Sciences (UVAS) Lahore, Pakistan on 23rd August 2017. (3). Molecular determinants of H9N2 avian influenza virus influencing antigenicity and receptor binding. Presented at CERAD (Centre of Excellence for Research on Avian Diseases), 3rd Symposium Recent Advances in Avian Disease Research. 31st August - 1st September 2017 at the Harbour Hotel, Alexandra Terrace, Guildford, Surrey, UK. (4) Overview of ongoing research on Poultry diseases in United Kingdom. Presented as at International poultry Stakeholders work on poultry biosecurity in Sri Lanka from 26th February to 3rd March 2017. (5). Evolutionary fitness of avian influenza viruses and development of improved vaccines and diagnostics. Presented as invited seminar speaker at RVC (Hawkshead Campus), UK. 19th October 2016.
Start Year 2012
 
Description Interferon stimulated genes 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution Hosted meetings and carried out preliminary experiments
Collaborator Contribution Contributed reagents and expertise
Impact Preliminary data was used to support an MSc project
Start Year 2016
 
Description Investigating the role of the SH protein of RSV in the duration of immunity 
Organisation Imperial College London
Department Department of Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution We provided initial data supporting the hypothesis that the small hydrophobic protein of bovine respiratory syncytial virus regulates the host's immune response by modulating the transcription factors NF-kB and STAT1 in bovine cells
Collaborator Contribution Based on our results, Imperial has began investigating the role of the small hydrophobic protein of human respiratory syncytial virus on human cells.
Impact Publication in progress titled "Regulation of the B cell activating factor BAFF by the small hydrophobic protein of respiratory syncytial virus".
Start Year 2017
 
Description JS - Production of monoclonal antibodies that recognise FMDV VP4 from mouse spleens 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution VLPs displaying the N-terminal 15 amino acids of FMDV were designed and produced at The Pirbright Institute and then mice were immunised with them. The spleens of the immunised mice were harvested and splenocytes stored. The response to the VP4 sequence was checked by ELISA and the mouse with the best response was selected to send the splenocytes for monoclonal production.
Collaborator Contribution The collaborators took the splenocytes obtained from the mouse experiments and carried out the fusions. They then screened the supernatants for positive wells that detected the VP4 sequence.
Impact Positive wells have been identified indicating some monoclonals that are specific for VP4 have been generated. These will be further screened for ability to recognise virus and to neutralise infection.
Start Year 2017
 
Description Molecular epidemiology of H9N2 avian influenza viruses in Vietnam and Pakistan. 
Organisation Royal Veterinary College (RVC)
Country United Kingdom 
Sector Academic/University 
PI Contribution Exchange of technical ideas, reagents and samples.
Collaborator Contribution Provision of field samples and data on avian influenza infection in poultry. This diseases is endemic in poultry in Pakistan.
Impact (1) Increased receptor binding avidity of H9N2 avian influenza viruses is associated with escape from antibody based immunity and enhanced zoonotic potential. presented by Joshua Sealy at GARAD and ZELS conferences in Haneoi, Vietnam from 17-27 January 2018.
Start Year 2008
 
Description Molecular epidemiology of H9N2 avian influenza viruses in Vietnam and Pakistan. 
Organisation University of Veterinary & Animal Sciences
Department Department of Microbiology
PI Contribution Exchange of technical ideas, reagents and samples.
Collaborator Contribution Provision of field samples and data on avian influenza infection in poultry. This diseases is endemic in poultry in Pakistan.
Impact (1) Increased receptor binding avidity of H9N2 avian influenza viruses is associated with escape from antibody based immunity and enhanced zoonotic potential. presented by Joshua Sealy at GARAD and ZELS conferences in Haneoi, Vietnam from 17-27 January 2018.
Start Year 2008
 
Description Morphological differences of Influenza A viruses 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution Exchange of research methods and reagents
Collaborator Contribution (1) Exchange of reagents and Research methods.(2) training of PhD students and Postdoctoral Scientists (3) use of research facilities
Impact Research output were presented at Avian Influenza A virus budding morphology: spherical or filamentous? presented at The European Scientific Working group on Influenza (ESWI) Riga, Latvia. 10 to 13 September 2017 (2) .Budding morphology of avian influenza A viruses. Microbiology Society, annual Conference 2017, 306 April, EICC, Edinburggh, UK
Start Year 2016
 
Description Mucosally Associated Invariant T cells (MAIT) in pig influenza model 
Organisation University of Oxford
Country United Kingdom 
Sector Academic/University 
PI Contribution Identified MAIT cells in pig Influenza model. Provide samples from infected and immunised protected animals
Collaborator Contribution Provide expertise in characterising MAIT: development of tetramer; Zell scanner technology allowing high resolution studies of cell phenotype and functions
Impact Joint PhD studentship with Paul Klenerman, University of Oxford
Start Year 2016
 
Description Nanoparticulate antigen delivery systems 
Organisation University College London Hospital
Country United Kingdom 
Sector Public 
PI Contribution Expertise in disease models, vaccine antigens and immune response evaluation
Collaborator Contribution Expertise in nanoparticulate formulations
Impact Several joint publications
Start Year 2012
 
Description Novel PRRS and swine influenza vaccines 
Organisation National Center for Genetic Engineering and Biotechnology (BIOTEC)
Country Thailand 
Sector Public 
PI Contribution Expertise in PRRS immunology and vaccine evaluation.
Collaborator Contribution Novel vaccine strains and SE Asian field strains
Impact Collaborative grant proposal funded under the BBSRC Newton Fund UK-China-Philippines-Thailand Swine and Poultry Research Initiative
Start Year 2017
 
Description Professor Sahyan Sharif, University of Guelph, Canada 
Organisation University of Guelph
Department Department of Pathobiology
Country Canada 
Sector Academic/University 
PI Contribution Collaboration between my team and Professor Sharif lead to new finding in Avian immunology, specifically in understanding the effects of nutrients on antigen presenting cells and T cells of chicken. We took the lead in studying the effects of some nutrients (Vitamin D) on the function of chicken T cells, performed most of the experiments in our laboratory. Meanwhile, we participated in studying the effects of Vitamin D on antigen presenting cells of chicken by performing some key experiments because we had access to some specific reagents (monoclonal antibodies) recognizing molecules expressed on chicken antigen presenting cells.
Collaborator Contribution Professor Sharif team took the lead in studying the effects of Vitamin D on antigen presenting cells of chicken, and contributed to experimental plan for studying the effects on chicken T cells.
Impact Two publications in 2015 and 2016: 1. Nitish Boodhoo, Shayan Sharif, Shahriar Behboudi. 1a,25(OH)2 Vitamin D3 Modulates Avian T Lymphocyte Functions without Inducing CTL Unresponsiveness. PLoS One. 2016 Feb 24;11(2):e0150134 2. Bahram Shojadoost, Shahriar Behboudi, Villanueva AI, Jennifer Brisbin, Ali Ashkar, Shayan Sharif. Vitamin D3 modulates the function of chicken macrophages. Res. Vet. Sci. 2015 June, 100: 45-51
Start Year 2014
 
Description Provision of PRRSV field strains 
Organisation Animal and Plant Health Agency
Country United Kingdom 
Sector Public 
PI Contribution Experimental heterologous PRRSV challenge model to assess the induction of broadly neutralizing antibodies. Model to assess dendritic cell tropism of PRRS viruses
Collaborator Contribution Provision of PRRSV-1 and -2 field strains
Impact Not yet
Start Year 2016
 
Description Provision of PRRSV field strains 
Organisation Kansas State University
Country United States 
Sector Academic/University 
PI Contribution Experimental heterologous PRRSV challenge model to assess the induction of broadly neutralizing antibodies. Model to assess dendritic cell tropism of PRRS viruses
Collaborator Contribution Provision of PRRSV-1 and -2 field strains
Impact Not yet
Start Year 2016
 
Description Re-annotation of Marek's Disease Virus 
Organisation U.S. Department of Agriculture USDA
Department Beltsville Agricultural Research Center
Country United States 
Sector Academic/University 
PI Contribution We are using bioinformatics analysis of high-throughput sequencing data in order to provide a better annotation of the genome of Marek's Disease Virus
Collaborator Contribution Our partners are sharing their extensive expertise of the genomics of MDV, and performing experimental validations
Impact No output yet
Start Year 2015
 
Description Reporter PRRS viruses 
Organisation Kansas State University
Country United States 
Sector Academic/University 
PI Contribution A new application for recombinant reporter PRRS viruses i.e. to use them as a high throughput screen for neutralizing (monoclonal) antibodies. Use as tools to assess the tropism of PRRSV for dendritic cell subsets and the functional consequences.
Collaborator Contribution Provision of plasmids encoding GFP-expressing PRRSV-1 and -2.
Impact Not yet
Start Year 2016
 
Description Research Collaboration with Nigeria (23rd - 28th January 2017) 
Organisation University of Nigeria
Country Nigeria 
Sector Academic/University 
PI Contribution Analysis of rare breed indigenous chicken in Nigeria.
Collaborator Contribution Helped with sampling and background information of the breed.
Impact Analysis is ongoing.
Start Year 2017
 
Description Retroviral-mediated genetic programming of porcine memory B cells 
Organisation AIMM Therapeutics
PI Contribution Provided a novel 'model' system to test their technology to isolate novel monoclonal antibodies
Collaborator Contribution To provide their patented platform technology to genetically program porcine memory B cells to enable the isolation of memory B cells
Impact Not yet
Start Year 2016
 
Description Swine haplotyping 
Organisation Gift Of Life
PI Contribution Prepared and shipped samples
Collaborator Contribution Sample analysis
Impact Data generation. Not multi-disciplinary
Start Year 2016
 
Description T folicullar helper cells in the pig influenza model 
Organisation University of Oxford
Department Nuffield Department of Medicine
Country United Kingdom 
Sector Academic/University 
PI Contribution Provide material from influenza infected/vaccinated protected and unprotected animals.
Collaborator Contribution Seph Borrow from Oxford University provide antibodies and advice as to how to identify and charcterise the TFH cells.
Impact Not yet
Start Year 2018
 
Description Test of broadly neutralising antibodies in pig influenza model 
Organisation Humabs Biomed SA
Country Switzerland 
Sector Private 
PI Contribution Tested the therapeutic use of broadly neutralizing antibodies in pig influenza model
Collaborator Contribution Provided the antibodies
Impact Manuscript submitted
Start Year 2015
 
Description The effect of the microbiota on immunity to swine infleunza 
Organisation University of Surrey
Country United Kingdom 
Sector Academic/University 
PI Contribution We wish to establish the gut and nasal microbiome in normal healthy pigs and how this is affected by influenza infection. We have collected gut samples from pigs at different stages of infection, as well as nasal swabs which we will provide to our collaborators in Surrey.
Collaborator Contribution Our collaborators in Surrey will perform the sequencing and bioinformatics analysis to determine the microbial communities present in the samples.
Impact No outcomes yet - we are in the process of analysing the samples.
Start Year 2017
 
Description The role of segment 3 in H9N2 avian influenza virus pathogenicity 
Organisation University of Edinburgh
Department The Roslin Institute
Country United Kingdom 
Sector Academic/University 
PI Contribution This is a Joint studentship project funded by The Pirbright Institute and The Roslin Institute. We identiifed molecular markers that are responsible for increase in virus pathogenicity and transmission.
Collaborator Contribution The Roslin Institute contributed both intellectually and by proving research training, samples and regents to achieve the prescribed objectives this project.
Impact 1. Oral Presentation Title: Identification of segment 3 sequence polymorphisms which alter shut off activity and pathogenicity of H9N2 avian influenza viruses in vivo. Anabel Clements, Saira Hussain, Holly Shelton, Paul Digard and Munir Iqbal. Global Alliance for Research Into Avian Diseases (GARAD). January 17th to 19th 2018. Fortuna Hotel, Lang Ha Street, Hanoi, VietNam 2. Poster title: What is the role of H9N2 avian influenza virus PA-X within an avian model?. Anabel Clements, Saira Hussain, Holly Shelton, Paul Digard & Munir Iqbal. UK- China CERAD (Centre of Excellence for Research on Avian Diseases), 3rd Symposium Recent Advances in Avian Disease Research. August 31st - 1st September 2017. The Harbour Hotel, Alexandra Terrace, Guildford, Surrey, UK. 3. Poster title: What is the role of H9N2 avian influenza virus PA-X within an avian model?. Anabel Clements, Saira Hussain, Holly Shelton, Paul Digard & Munir Iqbal. Microbiology Society Annual Conference. April 3rd-6th 2017. Edinburgh International Conference Centre (EICC), Edinburgh, UK. 4. Oral Presentation title: Identification of sequence polymorphisms in segment 3 of H9N2 avian influenza viruses that affect PA-X activity. Anabel Clements, Saira Hussain, Holly Shelton, Paul Digard & Munir Iqbal. Focused Meeting 2016: Molecular Biology and Pathogenesis of Avian Viruses. September 27th-29th 2016. Charles Darwin House, London, UK. 5. Oral Presentation title: The role of segment 3 in H9N2 avian influenza virus pathogenicity. Anabel Clements, Saira Hussain, Holly Shelton, Munir Iqbal & Paul Digard. UK -Taiwan BBSRC Partnering Award workshop: Improving Control of influenza viruses. 26th-27th April 2016. Taipei, Taiwan. 6. Oral Presentation title: Identification of sequence polymorphisms in segment 3 of H9N2 avian influenza viruses that affect PA-X activity. Anabel Clements, Saira Hussain, Holly Shelton, Munir Iqbal & Paul Digard. Microbiology Society Annual Conference 2016; virus Workshop: Negative strand RNA Viruses. 21st-24th March 2016. Arena and Convention Centre, Liverpool, UK. 7. Poster title: The role of segment 3 in H9N2 influenza virus pathogenicity. Anabel Clements, Saira Hussain, Holly Shelton, Munir Iqbal & Paul Digard. 7th Orthomyxovirus research conference. 16th-18th September 2015. Ecole Nationale Veterinaire de Toulouse, Toulouse, France. 8. Poster title: The role of segment 3 in H9N2 influenza virus pathogenicity. Anabel Clements, Saira Hussain, Holly Shelton, Munir Iqbal & Paul Digard. Edinburgh Infectious Diseases Annual Symposium. 20th May 2015. Royal College of Physicians, Edinburgh, UK.
Start Year 2014
 
Description USDA Culicoides 
Organisation U.S. Department of Agriculture USDA
Department National Animal Disease Center
Country United States 
Sector Public 
PI Contribution Combined data for Culicoides genome project to enable publication in high impact journal.
Collaborator Contribution Transcriptomics data for vector competence analysis and genome build.
Impact Combined data for Culicoides genome project to enable publication in high impact journal.
Start Year 2014
 
Description Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry 
Organisation Beijing Academy of Agriculture and Forestry Sciences
Country China 
Sector Academic/University 
PI Contribution Undertake joint research work to investigate to identify the molecular determinants that are critical for enhancing potency and efficacy of vaccines, as well as those that are associated with heightened zoonotic risk.
Collaborator Contribution Provision of samples, reagents and research facilities
Impact Published joint paper (1)ian Xu, Xixi Zhang, Shuanghai Zhou, Junjun Shen,Dawei Yang, Jing Wu, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua E. Sealy, Munir Iqbal&Yongqing Li (2017). A DNA aptamer efficiently inhibits the infectivity of Bovine herpesvirus 1 by blocking viral entry. Scientific Reports. 7: 11796. doi:10.1038/s41598-017-10070-1. (2) Jian Xu, Jing Wu, Houjun He, Junjun Shena, Dawei Yang, Xixi Zhang, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua Sealy, Munir Iqbal & Yongqing Li (2017). Bovine single chain Fv antibody inhibits bovine herpesvirus-1 infectivity by targeting viral glycoprotein D. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-017-8566-0. (2)
Start Year 2017
 
Description Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry 
Organisation China Agricultural University (CAU)
Department College of Veterinary Medicine
PI Contribution Undertake joint research work to investigate to identify the molecular determinants that are critical for enhancing potency and efficacy of vaccines, as well as those that are associated with heightened zoonotic risk.
Collaborator Contribution Provision of samples, reagents and research facilities
Impact Published joint paper (1)ian Xu, Xixi Zhang, Shuanghai Zhou, Junjun Shen,Dawei Yang, Jing Wu, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua E. Sealy, Munir Iqbal&Yongqing Li (2017). A DNA aptamer efficiently inhibits the infectivity of Bovine herpesvirus 1 by blocking viral entry. Scientific Reports. 7: 11796. doi:10.1038/s41598-017-10070-1. (2) Jian Xu, Jing Wu, Houjun He, Junjun Shena, Dawei Yang, Xixi Zhang, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua Sealy, Munir Iqbal & Yongqing Li (2017). Bovine single chain Fv antibody inhibits bovine herpesvirus-1 infectivity by targeting viral glycoprotein D. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-017-8566-0. (2)
Start Year 2017
 
Description Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry 
Organisation Chinese Center for Disease Control and Prevention (China CDC)
Country China 
Sector Public 
PI Contribution Undertake joint research work to investigate to identify the molecular determinants that are critical for enhancing potency and efficacy of vaccines, as well as those that are associated with heightened zoonotic risk.
Collaborator Contribution Provision of samples, reagents and research facilities
Impact Published joint paper (1)ian Xu, Xixi Zhang, Shuanghai Zhou, Junjun Shen,Dawei Yang, Jing Wu, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua E. Sealy, Munir Iqbal&Yongqing Li (2017). A DNA aptamer efficiently inhibits the infectivity of Bovine herpesvirus 1 by blocking viral entry. Scientific Reports. 7: 11796. doi:10.1038/s41598-017-10070-1. (2) Jian Xu, Jing Wu, Houjun He, Junjun Shena, Dawei Yang, Xixi Zhang, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua Sealy, Munir Iqbal & Yongqing Li (2017). Bovine single chain Fv antibody inhibits bovine herpesvirus-1 infectivity by targeting viral glycoprotein D. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-017-8566-0. (2)
Start Year 2017
 
Description Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry 
Organisation Francis Crick Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution Undertake joint research work to investigate to identify the molecular determinants that are critical for enhancing potency and efficacy of vaccines, as well as those that are associated with heightened zoonotic risk.
Collaborator Contribution Provision of samples, reagents and research facilities
Impact Published joint paper (1)ian Xu, Xixi Zhang, Shuanghai Zhou, Junjun Shen,Dawei Yang, Jing Wu, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua E. Sealy, Munir Iqbal&Yongqing Li (2017). A DNA aptamer efficiently inhibits the infectivity of Bovine herpesvirus 1 by blocking viral entry. Scientific Reports. 7: 11796. doi:10.1038/s41598-017-10070-1. (2) Jian Xu, Jing Wu, Houjun He, Junjun Shena, Dawei Yang, Xixi Zhang, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua Sealy, Munir Iqbal & Yongqing Li (2017). Bovine single chain Fv antibody inhibits bovine herpesvirus-1 infectivity by targeting viral glycoprotein D. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-017-8566-0. (2)
Start Year 2017
 
Description Understanding antigenic diversity, zoonotic potential and immunological prevention of avian influenza viruses affecting poultry 
Organisation University of Glasgow
Department Institute of Biodiversity, Animal Health and Comparative Medicine
Country United Kingdom 
Sector Multiple 
PI Contribution Undertake joint research work to investigate to identify the molecular determinants that are critical for enhancing potency and efficacy of vaccines, as well as those that are associated with heightened zoonotic risk.
Collaborator Contribution Provision of samples, reagents and research facilities
Impact Published joint paper (1)ian Xu, Xixi Zhang, Shuanghai Zhou, Junjun Shen,Dawei Yang, Jing Wu, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua E. Sealy, Munir Iqbal&Yongqing Li (2017). A DNA aptamer efficiently inhibits the infectivity of Bovine herpesvirus 1 by blocking viral entry. Scientific Reports. 7: 11796. doi:10.1038/s41598-017-10070-1. (2) Jian Xu, Jing Wu, Houjun He, Junjun Shena, Dawei Yang, Xixi Zhang, Xiaoyang Li, Meiling Li, Xiufen Huang, Joshua Sealy, Munir Iqbal & Yongqing Li (2017). Bovine single chain Fv antibody inhibits bovine herpesvirus-1 infectivity by targeting viral glycoprotein D. Applied Microbiology and Biotechnology. https://doi.org/10.1007/s00253-017-8566-0. (2)
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Agricultural Research Centre
Department Veterinary Serum and Vaccine Research Institute
Country Egypt 
Sector Public 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Francis Crick Institute
Country United Kingdom 
Sector Charity/Non Profit 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Imperial College London
Department MSk Lab
Country United Kingdom 
Sector Academic/University 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Imperial College London
Country United Kingdom 
Sector Academic/University 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Suez Canal University
Country Egypt 
Sector Academic/University 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation University of Tokyo
Department International Research Center for Infectious Diseases
Country Japan 
Sector Hospitals 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Understanding diversity of avian influenza viruses and improvement disease control in poultry and humans 
Organisation Zagazig University
Country Egypt 
Sector Academic/University 
PI Contribution This project helped my research group to establish research collboration between the Pirbright Institute and the scientists at the collaborating laboratories listed above and an excellent opportunity for building a joint "ONE HEALTH" platfor. I am sharing knowledge, reagents and techniques to improve efficacious vaccines and diagnostic tools against avian influenza viruses. The easy availability of better control tools against avian influenza viruses will aid in the reduction of poultry production losses and thus be important for global food security and improved animal welfare whilst also reducing zoonotic transmission to humans.
Collaborator Contribution Each collaborating institute and team member has specific expertise and facilities in areas relevant to this research programme. Collaborators in different institutions have extensive experience collecting field samples and implementing vaccination and large scale surveillance programmes. Each partner institutions have unique experience in monitoring the endemic prevalence of many animal diseases including avian influenza in both domestic and wild birds from which UK is under constant threat. The partners are sharing knowledge, expertise and facilities to investigate fundamental biology of influenza viruses and predict their epidemiological relevance, to assess potential risks and to devise appropriate intervention strategies. The knowledge, reagents and field samples will be available through this partnership. which will be essential for development of informed control policies and tools(vaccines and diagnostics) that may contribute to reducing the ever increasing threat from endemic, emerging and re-emerging influenza virus variants posing risk to animals and humans. Also, All researchers involved will benefit through ongoing professional development and advanced training in new technologies and new systems-level approaches to disease management. Exchange visits of researchers from collaborating laboratories to undertake training in a number of disciplines including disease surveillance, molecular virology, epidemiology, immunology, vaccinology and diagnostics will have a tangible impact on capacity building and training of next generation researchers in control of infectious animal and zoonotic diseases.
Impact The ultimate aims of this collaboration are to reduce the impact of influenza virus on poultry production and reduce their zoonotic transmission to humans. The collaboration will improve resources and capacity for disease control systems. Achieving these specific goals will prove a step-change in AIV disease management and increase in poultry productivity directly drives economic prosperity of farmers and allied communities. The will directly impact on socio-economic well-being of partner countries.
Start Year 2017
 
Description Use of fluorescent virions to isolate PRRSV-specific porcine B cells 
Organisation University of Minnesota
Country United States 
Sector Academic/University 
PI Contribution Deployment of a novel technology to facilitate isolation of PRRSV neutralizing monoclonal antibodies
Collaborator Contribution Provision of a method to fluorescently tag purified virions to label PRRSV-specific B cells
Impact None to report to date
Start Year 2016
 
Description Vaccinia 
Organisation Defence Science & Technology Laboratory (DSTL)
Country United Kingdom 
Sector Public 
PI Contribution Generated and analysed DNA- and RNA-sequencing data from vaccinia samples with bespoke in-house pipelines
Collaborator Contribution Generated DNA and RNA vaccinia samples
Impact The collaboration aims to better understand the interaction between vaccina virus and its host(s), through the use of modern high-throughput techniques. No outputs yet
Start Year 2016
 
Description A talk at Surrey University 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Under- and postgraduate students attended a talk I gave at the Department of Biological Sciences in Surrey. There was a lively discussion afterwards and as a result I have established a new collaboration.
Year(s) Of Engagement Activity 2018
 
Description A talk at a symposium entitled: Using the 3Rs to support good science 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I gave a talk at a Symposium held at The Pirbright Institute entitled: "Using the 3Rs to support good science". The talk was entitled: "A chicken primary B cell culture model to study the pathogenesis and improve the control of immunosuppressive viruses of poultry"
Year(s) Of Engagement Activity 2017
 
Description A talk at the British Poultry Diseases Group meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Industry/Business
Results and Impact The Poultry Diseases Group meet quarterly and is comprised of representatives from private veterinary practices, vaccine and pharmaceutical industries, and the Animal and Plant Health Agency (APHA). I gave a talk at one of the meetings outlining my research to date and future directions.
Year(s) Of Engagement Activity 2017
 
Description AB Improving Approaches to Prevent and Control Viral Diseases of Livestock and Poultry 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Batra, A., Maier, H.J., Britton P., Hiscox, J.A., Fife, M.S., 2015. PI3K/AKT signalling during infectious bronchitis virus infection. Food Security: Improving Approaches to Prevent and Control Viral Diseases of Livestock and Poultry, British Council Research Links Workshop, Istanbul, Turkey (Oral presentation)
Year(s) Of Engagement Activity 2015
 
Description Centre of excellence for research on avian diseases (CERAD) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I was invited to present my work at the UK-China Centre of excellence for research on avian diseases (CERAD) meeting attended by researchers from UK, China and Thailand. There was lots of discussion about the research and future directions, including potential collaborations.
Year(s) Of Engagement Activity 2017
 
Description Chair of organising Committee for Wellcome Trust-Animal Genetics and Diseases 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact This meeting brought together specialists working on the interface between genomics, genetic engineering and infectious disease with the aims of improving animal and human health and welfare.

Scientific sessions included:
Genetics of immune responses and disease resistance
Genetically engineered livestock (including genome editing)
Quantitative genetics and epigenetics applied to disease
Epidemiology and pathogen evolution
Bioinformatics, comparative and functional genomics
Precision medicine of animal companions
Year(s) Of Engagement Activity 2017
URL https://coursesandconferences.wellcomegenomecampus.org/events/item.aspx?e=635&dm_i=2SUU,HOGH,4R4AW1,...
 
Description Combating avian influenza through systematic analysis of antigenic drift, genetic variation, and development of novel diagnostic tools and vaccines. Presented at as PI of the ZELS project at ZELS grant holders and Stakeholders meeting in Hanoi, Vietnam 23th-24th January 2017. 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Engagement and dissemination of research outcome to representatives of research funding bodies such as Department for International Development (DFID), Medical Research Council (MRC) and the researchers working on different research projects funded by BBSRC under Zoonoses and Emerging Livestock Systems (ZELS).
Year(s) Of Engagement Activity 2018
 
Description Diamond open day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Over 300 people visited our stand at the open day, in which we described the importance of our work for food security and public health. Children from all ages were involved and learned about viruses and played with the huge virus particle that we had produced. One of the major impact was that we described to several people why we work on some viruses that we do not have them in the UK, and the importance of the work for the UK and UK economy and public health was explained.
Year(s) Of Engagement Activity 2017
URL http://www.diamond.ac.uk/Public/VisitUs.html
 
Description Human Infection Challenge Models Network 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Policymakers/politicians
Results and Impact HIC-Vac is an international network of researchers who are developing human infection challenge (HIC) studies to accelerate the development of vaccines against pathogens of high global impact. The goal is to foster an engaged and interactive community of international researchers to promote open sharing of knowledge and expertise, generate new ideas, support and share best practice, and form new cross-discipline collaborations.
Year(s) Of Engagement Activity 2018
 
Description Immunology workshop University of Surrey 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact 2 hour practical discussion workshop on Immunology of viral infection
Year(s) Of Engagement Activity 2017
 
Description Innovate Guildford Outreach (12th March) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact With amazing exhibits, activities, workshops, talks and competitions, it's FREE to attend. Explore the four zones: Engineering, One Health, Digital Media and Future Living, experience interactive performances, hear inspiring speakers (including leading gaming pioneer Peter Molyneux and animal health pioneer Professor Nick Bacon) and take in an amazing atmosphere. Theatre, music and comedy will feature through the day, with everything from how technology might have affected Shakespeare's plays to risky recycling and photographic secrets.
Year(s) Of Engagement Activity 2017
URL https://www.guildford.gov.uk/innovateguildford
 
Description Innovate Guilford 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Dr Broadbent gave a talk at 'Innovate Guilford' Festival outlining the work we are doing with Avian Virus Vaccines, including those against IBDV. The aim was to raise public awareness on different avian viruses, and genetically modified vaccines.
Year(s) Of Engagement Activity 2017
 
Description International Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Global Alliance for Research on Avian Diseases conference health in Hanoi 17-19 January 2018. Purpose of the conference was to establish global partnerships and links between academic researchers and the poultry industry to improve joined up work in regards to preventing, controlling and
combating avian diseases. The conferences provided a forum for discussion and exchange of ideas around the single theme of exploiting available opportunities to deal with emerging and existing infectious avian diseases in poultry production systems and to safeguard food supplies and human health.
Conference speakers discuused basic, applied and commercial aspects of research on avian diseases including:
• Evolution and Epidemiology of Avian Pathogens
• Host-Pathogen Interactions - Virulence and Pathogenicity
• Pathogen Mechanisms of Immune Evasion
• Next Generation Disease Control Strategies
• Vaccines
• Immunity
• Diagnostics
• Zoonoses and Socio-economic impacts of avian diseases
• Global Research Opportunities and Threats
Year(s) Of Engagement Activity 2018
URL http://www.theeventsportal.com/GARAD-2018/3/Home
 
Description Invited Presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Invited talk titled 'Identification of viral targets of host T cell immunity for development of livestock vaccines' presented at the British Society for Immunology Congress, Brighton, UK.
Year(s) Of Engagement Activity 2010,2017
URL https://www.bsicongress.com/
 
Description Invited Presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Invited talk entitled 'Identification of viral targets of host T cell immunity for development of livestock vaccines' presented at the International Symposium on Human and Veterinary Viruses 2018: From Research to Application, Bangkok, Thailand.
Year(s) Of Engagement Activity 2018
URL http://www.biotec.or.th/virus-symposium2018/index.php
 
Description Invited Presentation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Invited talk titled 'Design and evaluation of biomimetic particulate formulations of viral antigens as veterinary vaccines' presented at VaxInLive 2018 Symposium, Lyon, France.
Year(s) Of Engagement Activity 2018
URL https://clarolineconnect.univ-lyon1.fr/icap_website/view/1228
 
Description Invited Presentation 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Presentations at the European PRRS Research Award ceremonies
Year(s) Of Engagement Activity 2016,2017
 
Description Lumpy Skin Disease D2R2 profile 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/politicians
Results and Impact Provided expert opinion to the assessment (D2R2) of the risk of lumpy skin disease virus (LSDV) to the UK.
Year(s) Of Engagement Activity 2017,2018
 
Description MDV conference Tours 2016 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact During the 11th International Symposium on Marek's Disease and Avian Herpesviruses, which took place from 6 to 9 July 2016, Paolo Ribeca gave a talk on "Attenuation of MDV: an RNA-seq based perspective".
Year(s) Of Engagement Activity 2016
 
Description MSc student visit 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact I hosted ten MSc students from University of Surrey, discussed avian research with them and demonstrated some laboratory techniques. The students gained an understanding of academic research and laboratory work.
Year(s) Of Engagement Activity 2018
 
Description Microbiology Society Annual conference (UK) 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Industry/Business
Results and Impact Poster or oral presentation at Microbiology Society annual conference, a major international event. The participants included researchers from many different areas of science. This provides a platform to promote research to a wider audience as well as discuss the key findings with experts in my own field.
Year(s) Of Engagement Activity 2010,2011,2012,2013,2014
 
Description Networking presentation-INRA, Paris, France 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact A presentation at INRA describing the role of the SH protein of BRSV in modulating immune responses in calves. This presentation resulted in a heated debate on the role of viral proteins in immune evasion and their activities in attenuated vaccines.
Year(s) Of Engagement Activity 2017
 
Description Nidovirus symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I presented my work at the Nidovirus Symposium, which is held every three years and brings together researchers from industry and academia from many different countries. I had some interesting discussions with other researchers and built my network.
Year(s) Of Engagement Activity 2017
 
Description Oral presentation at the Global Alliance for Research on Avian Disease meeting in Hanoi, Vietnam 15th -19th Jan 2018 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact A talk was given on the PB1-F2 function in avian influenza infection of avian hosts at the GARAD meeting. This meeting was attended by approximately 120 researchers and poultry industry members from a wide international geographic region.
Year(s) Of Engagement Activity 2018
 
Description Organisation of Influenza meeting at Roslin Institute 21st-22nd November 2017 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Along with Paul Digard (Edinburgh) and Edward Hutchinson (Glasgow) I helped to organize, plan and run a Influenza update meeting which ran for 2 days at The Roslin Institute in November 2017. The symposium was attended by approximately 60 influenza researchers from the UK. Talks were given by a range of people with student talks and posters being awarded prizes. This was supported by Medimmune and the Microbiology Society.
Year(s) Of Engagement Activity 2017
 
Description Presentation at Global Alliance for Research on Avian Diseases Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Presentation of my research at the Global Alliance for Research on Avian Diseases Conference in Vietnam to approximately 80 conference participants resulted in discussions about future research directions.
Year(s) Of Engagement Activity 2018
 
Description Presentation at RIVR meetings 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Presentation of my research at Recently Independent Virology Researchers (RIVR) meetings in 2016, 2017 and 2018. I discussed my work and future collaborations with other virology researchers working in the UK.
Year(s) Of Engagement Activity 2016,2017,2018
 
Description Presentation by RH at Nidovirus symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact PhD student presented our data at the 14th international Nidovirus symposium. He discussed results and potential for future collaboration with other scientists in the field.
Year(s) Of Engagement Activity 2017
 
Description Presentation: BSI AGM: Immune regulation by the SH protein of BRSV 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Presentation at the BSI's AGM to describe the data generated regarding the role of the SH protein of BRSV in regulating immune responses after natural infections. Initiated a debate about the role of the SH protein of human RSV in immune regulation.
Year(s) Of Engagement Activity 2017
 
Description Reverse genetics seminar (University of Surrey) 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact A seminar was given based upon the reverse genetics system of infectious bronchitis virus. The audience was a group of 10 -15 MSc students from University of Surrey. The students were engaged, asked questions about the research and careers in science. We were asked to repeat the seminar for the next year's intake of students.
Year(s) Of Engagement Activity 2013,2014,2015,2016,2017
 
Description Reverse genetics seminar to MSc students 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact A seminar was presented describing reverse genetics of viruses, including infectious bronchitis virus and the application of reverse genetics in research. The students were interested and engaged, participating in activities and asking questions. We were invited to present the seminar in subsequent years for new student intakes.
Year(s) Of Engagement Activity 2013,2014,2015,2016,2018
 
Description STEM Outreach Event (Farnborough, 9th November) 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact See web link
Year(s) Of Engagement Activity 2016
 
Description Southampton Lecture 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Undergraduate students
Results and Impact Lectures on Genomic Immunology and Food Security as part of an undergraduate course to molecular biologists
Year(s) Of Engagement Activity 2015,2016,2017
 
Description Surrey Open day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Undergraduate students
Results and Impact Described the research activity of the Pirbright Institute for students from school, graduate and postgraduate students, and provided information on research facilities in the institute.
Year(s) Of Engagement Activity 2017
URL https://www.surrey.ac.uk/opendays/vet
 
Description Talk at the Merseyside BSI Affinity group 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact I described the methods we used to identifyTissue resident memory cells in pigs, which sparked a lively discussion and interest in the technique. As a result I have established two collaborations with colleagues from Liverpool.
Year(s) Of Engagement Activity 2018
 
Description Television Interview Ulaanbaatar 
Form Of Engagement Activity A broadcast e.g. TV/radio/film/podcast (other than news/press)
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Media (as a channel to the public)
Results and Impact I gave a television interview to Mongolian national television while attending a workshop in Ulaanbaatar. The interview was focused on the collaboration between the State Central Veterinary Laboratory in Ulaanbaatar and The Pirbright Institute, particularly the work we are undertaking on sheeppox and goatpox.
Year(s) Of Engagement Activity 2017
 
Description Television interview Ulaanbaatar 
Form Of Engagement Activity A press release, press conference or response to a media enquiry/interview
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact I was interviewed by UB television while on a collaborative visit to the State Central Veterinary Laboratory in Mongolia. I spoke about the collaboration between The Pirbright Institute and the SCVL, and how this benefits the Mongolian agricultural sector.
Year(s) Of Engagement Activity 2017
 
Description Tour of Pirbright Research facilities for CLA group 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact The CLA is the membership organisation for owners of land, property and businesses in rural England and Wales. The CLA came for a tour of the Jenner building facilities at The Pirbright Institute. I led a tour of our building for the group and described the work undertaken by the avian research programme here.
Year(s) Of Engagement Activity 2017
 
Description UK-India bioinformatics workshop 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact On February 9 2018 Pirbright organised a "UK-India bioinformatics workshop" in order to foster ties between Indian and UK bioinformaticians working in the field of livestock and crop research. Paolo Ribeca gave a talk on "Bioinformatics and sequencing at Pirbright".
Year(s) Of Engagement Activity 2018
 
Description University of Surrey School of Veterinary Medicine Open Day 
Form Of Engagement Activity Participation in an open day or visit at my research institution
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Presentation of work conducted at The Pirbright Institute
Year(s) Of Engagement Activity 2016,2017
 
Description Veterinary Research Club 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Presentation of the data generated and its importance in understanding immune responses to vaccination and natural infections. About 50 veterinarians and researchers working in veterinary sciences attended the one-day meeting, which initiated discussions about the possibility of generating new vaccines against BRSV.
Year(s) Of Engagement Activity 2017
 
Description Virtual video tour of the animal facilities in Pirbright 
Form Of Engagement Activity Engagement focused website, blog or social media channel
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Contributed to public understanding of animal research by being interviewed on the virtual video tour of the animal facilities in Pirbright - one of only four such initiatives in the UK.
Year(s) Of Engagement Activity 2017
 
Description Workshop with MSc students in Veterinary Microbiology 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Postdoctoral scientists employed on projects participated in practical 1-day workshop with MSc students from the University of Surrey
Year(s) Of Engagement Activity 2017
 
Description a talk at the Recently Independent Virology Investigators meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact I gave a talks outlining my current research and future plans.
Year(s) Of Engagement Activity 2015,2016,2017
 
Description organised workshops at Microbiology Society annual conferences 2014 - 2018 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact I co-organised virology division workshops on viral evolution and diversity and RNA viruses with colleagues from the virology division. The workshops were held at the annual Microbiology Society conferences in spring each year. Organisation involved selecting abstracts for oral or poster presentation, ordering the presentations and chairing the sessions.
Year(s) Of Engagement Activity 2015,2016,2017,2018
 
Description talk at a UK-China symposium on recent advances in avian disease research 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact I gave a talk entitled: Differential gene expression in chicken primary B cells infected ex vivo with attenuated and very virulent strains of infectious bursal disease virus (IBDV)
Year(s) Of Engagement Activity 2017